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Gene information
General information
TC008530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451492.1: 293002..311387
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008530
    iBeetle screen iB_01362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008530 RA
      MAKQSSVKS KRKLINPVF HLQTKEEDD NQLTKGMIK 
IVRRTGQLN LSGRSLAHV PEKMFTMYE LTEEDTAKY 
IDFNKEPEE ENWWQFKPL NYLDLSSNV LQEIPGKIG 
MFEDLTALN LQDNNITSL PPEICNLTK LTKLNLSHN 
KINLLPMEF YKLVELQVL SLAHNNLEK ISKNFADLV 
MLQQLDLSH NILTKLPPG MGFLVRLTE INLSHNKLI 
ELPPDIVNL RGLLKLDVT HNDLVYLPK MGELAKLQF 
LYAQHNNIE EIPDFEGCT HLQQVYFGN NYIKEVTTD 
FCENMSNLK ILDLRDNKI EQIPNEIAM LQHLIRLDL 
TNNDLTDLP NSLGLLAHL QNLQLEGNK LKKIRGDII 
KGGTMRILK HLKEQLDDE DLESVPKVS TMQHEAKTF 
PDKYTIKHS RSLNVTMKQ LTSVPDDVF EEAKLAEVT 
IVDLCKNKL PSVPNGIQL VAENLTELN LSMNAISEI 
PEFISNCIK LKYFDLGNN LLSDLPECL SSLVGLREL 
VLSNNRFVH IPDCVYSMV GLEILLASD NKITDINVE 
GLKNLTRIA TLDLTNNNM SHIPPELGN VTQLRTLEL 
RGNCFRQPR YAILEQGTA SVLSYLRDR IPK
    CDS sequence
    • >TC008530 RA
      atggccaaa caatcaagc gttaaatct aaacgtaaa 
ctaataaat ccagttttt catctacaa acaaaagaa 
gaagatgac aatcaacta acaaaaggt atgatcaaa 
attgttcga aggacggga cagctcaac ttatcggga 
cgtagtttg gcccatgta cctgaaaaa atgtttact 
atgtatgaa ttaacagag gaagacact gcgaaatat 
atagacttc aacaaagag cccgaggag gaaaactgg 
tggcagttt aaaccctta aattacttg gacctaagc 
tcaaacgta ctgcaagag attcccgga aaaattgga 
atgtttgag gacttgact gcactcaat ttgcaagat 
aacaacata acaagttta ccccctgaa atttgtaat 
ctaaccaag ttaacaaaa ctaaacctc agccataac 
aagattaat ttgttgcca atggaattt tacaagtta 
gtggagtta caggtcctt tcattagct cataacaac 
ttggaaaaa atctcgaaa aactttgcc gatttggtt 
atgctacaa caattggac ttgtctcat aatatttta 
accaaatta ccaccagga atggggttt ttagtgcga 
ctaactgaa ataaatctc tcacataac aaactaatt 
gaattacca cccgatatt gttaactta aggggtctc 
ttgaaattg gatgttact cacaatgac ttggtttac 
ttgccaaaa atgggcgaa cttgccaag ttacagttt 
ttgtatgcc caacacaat aatattgag gaaattcca 
gattttgag ggctgtaca cacttacag caagtctat 
tttggcaac aattacata aaagaagtt actaccgat 
ttttgtgaa aacatgtca aatttaaaa attcttgat 
ttgcgagac aataaaatc gagcaaatt cccaatgaa 
atcgcgatg ttgcaacac ttgattcga ctcgattta 
accaataat gatttgaca gatcttccc aattcattg 
ggtttgtta gcgcacttg caaaatctt caattggaa 
ggtaacaag ttgaaaaaa atcagaggt gatattatt 
aaaggagga acaatgcgc attttgaaa catttgaag 
gagcagctt gatgatgaa gaccttgag agtgttcct 
aaagtctcc actatgcaa catgaagct aaaacattt 
cccgataaa tacactatc aaacattca cgttctttg 
aatgttaca atgaagcag cttacttca gtacctgat 
gatgttttc gaggaagcc aaattagca gaagttact 
attgttgac ttgtgtaaa aataaacta ccttcagtg 
cctaatggg atacaatta gtggccgaa aatttgacc 
gaattaaat ttgtctatg aatgctatt agcgaaatt 
cctgaattt atcagcaat tgtataaaa ctgaaatat 
ttcgatttg ggcaacaat ttattgtcg gatttgccg 
gaatgttta agttcattg gttggttta agagaactt 
gtactaagt aacaataga tttgttcac ataccagat 
tgtgtgtac agtatggta gggttagaa atactttta 
gctagtgat aacaaaata actgatatt aatgtggag 
ggactgaaa aatcttaca agaattgca actttagat 
ttgaccaac aacaacatg agtcatata ccacctgaa 
ctggggaat gtcactcaa ctcaggaca ctggagtta 
cgaggtaat tgctttaga caaccgagg tacgcaatt 
ttagaacaa gggactgct tccgtacta agttatttg 
agggataga attcctaaa tag
    mRNA sequence
    • >TC008530 RA
      tcggaaatg gcgttctaa taagaaaat tgatacaac 
ttgcggtat aaaagtccc ggtataaaa gttcatcat 
tatggagca aatattcta tgctaagat caaccatca 
atctacgag agggaattg acattagta aatttccaa 
gcgtcatac cttatttga aacgaaaag gatagaggt 
catttcaag gttcatgat gtcacaaca aaaacgaaa 
ggttatgtt tctgtttga ttaatcgat atttttccg 
ttaaagata ttccacaat ggccaaaca atcaagcgt 
taaatctaa acgtaaact aataaatcc agtttttca 
tctacaaac aaaagaaga agatgacaa tcaactaac 
aaaaggtat gatcaaaat tgttcgaag gacgggaca 
gctcaactt atcgggacg tagtttggc ccatgtacc 
tgaaaaaat gtttactat gtatgaatt aacagagga 
agacactgc gaaatatat agacttcaa caaagagcc 
cgaggagga aaactggtg gcagtttaa acccttaaa 
ttacttgga cctaagctc aaacgtact gcaagagat 
tcccggaaa aattggaat gtttgagga cttgactgc 
actcaattt gcaagataa caacataac aagtttacc 
ccctgaaat ttgtaatct aaccaagtt aacaaaact 
aaacctcag ccataacaa gattaattt gttgccaat 
ggaatttta caagttagt ggagttaca ggtcctttc 
attagctca taacaactt ggaaaaaat ctcgaaaaa 
ctttgccga tttggttat gctacaaca attggactt 
gtctcataa tattttaac caaattacc accaggaat 
ggggttttt agtgcgact aactgaaat aaatctctc 
acataacaa actaattga attaccacc cgatattgt 
taacttaag gggtctctt gaaattgga tgttactca 
caatgactt ggtttactt gccaaaaat gggcgaact 
tgccaagtt acagttttt gtatgccca acacaataa 
tattgagga aattccaga ttttgaggg ctgtacaca 
cttacagca agtctattt tggcaacaa ttacataaa 
agaagttac taccgattt ttgtgaaaa catgtcaaa 
tttaaaaat tcttgattt gcgagacaa taaaatcga 
gcaaattcc caatgaaat cgcgatgtt gcaacactt 
gattcgact cgatttaac caataatga tttgacaga 
tcttcccaa ttcattggg tttgttagc gcacttgca 
aaatcttca attggaagg taacaagtt gaaaaaaat 
cagaggtga tattattaa aggaggaac aatgcgcat 
tttgaaaca tttgaagga gcagcttga tgatgaaga 
ccttgagag tgttcctaa agtctccac tatgcaaca 
tgaagctaa aacatttcc cgataaata cactatcaa 
acattcacg ttctttgaa tgttacaat gaagcagct 
tacttcagt acctgatga tgttttcga ggaagccaa 
attagcaga agttactat tgttgactt gtgtaaaaa 
taaactacc ttcagtgcc taatgggat acaattagt 
ggccgaaaa tttgaccga attaaattt gtctatgaa 
tgctattag cgaaattcc tgaatttat cagcaattg 
tataaaact gaaatattt cgatttggg caacaattt 
attgtcgga tttgccgga atgtttaag ttcattggt 
tggtttaag agaacttgt actaagtaa caatagatt 
tgttcacat accagattg tgtgtacag tatggtagg 
gttagaaat acttttagc tagtgataa caaaataac 
tgatattaa tgtggaggg actgaaaaa tcttacaag 
aattgcaac tttagattt gaccaacaa caacatgag 
tcatatacc acctgaact ggggaatgt cactcaact 
caggacact ggagttacg aggtaattg ctttagaca 
accgaggta cgcaatttt agaacaagg gactgcttc 
cgtactaag ttatttgag ggatagaat tcctaaata 
gtttctagt catagtttt attttctgt tttatttcg 
taggccttg atcgaaacg tgtgattag caggatcaa 
attttgata tgattgccc ttttaacca tttgcaaat 
gttacaggg atatgcatt actttgaaa atgtaatcg 
aaaatggtt tatttgttt ttattgtat ttcttttcg 
tttttcttc ctctgtttt ccattttct gtgtatgag 
ataggcctg ctttagaaa attgttcct tcatttctt 
acttgcact gttccgaac actttttag gtttgatag 
aacaaagat agaagtttt ttttatata tcgtcggat 
gttgcaaaa ataagataa ctcaatttt gttgtcgct 
aacagtgaa acttatgaa ggcatgtgc ataaaatag 
catgggtaa ttttttgca ataaccgat ttttaactg 
ttgtgtaaa agtttaata gttgtcttc tccgaatta 
ctgcttttt gcaataacg aaacatata gggtgttcc 
atctaaacc ccacattag aaaattgca agttttaat 
aattaagta tattcatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY