Status
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Gene information
General information
TC008530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451492.1: 293002..311387
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008530
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008530 RA
      MAKQSSVKS KRKLINPVF HLQTKEEDD NQLTKGMIK 
      IVRRTGQLN LSGRSLAHV PEKMFTMYE LTEEDTAKY 
      IDFNKEPEE ENWWQFKPL NYLDLSSNV LQEIPGKIG 
      MFEDLTALN LQDNNITSL PPEICNLTK LTKLNLSHN 
      KINLLPMEF YKLVELQVL SLAHNNLEK ISKNFADLV 
      MLQQLDLSH NILTKLPPG MGFLVRLTE INLSHNKLI 
      ELPPDIVNL RGLLKLDVT HNDLVYLPK MGELAKLQF 
      LYAQHNNIE EIPDFEGCT HLQQVYFGN NYIKEVTTD 
      FCENMSNLK ILDLRDNKI EQIPNEIAM LQHLIRLDL 
      TNNDLTDLP NSLGLLAHL QNLQLEGNK LKKIRGDII 
      KGGTMRILK HLKEQLDDE DLESVPKVS TMQHEAKTF 
      PDKYTIKHS RSLNVTMKQ LTSVPDDVF EEAKLAEVT 
      IVDLCKNKL PSVPNGIQL VAENLTELN LSMNAISEI 
      PEFISNCIK LKYFDLGNN LLSDLPECL SSLVGLREL 
      VLSNNRFVH IPDCVYSMV GLEILLASD NKITDINVE 
      GLKNLTRIA TLDLTNNNM SHIPPELGN VTQLRTLEL 
      RGNCFRQPR YAILEQGTA SVLSYLRDR IPK
    CDS sequence
    • >TC008530 RA
      atggccaaa caatcaagc gttaaatct aaacgtaaa 
      ctaataaat ccagttttt catctacaa acaaaagaa 
      gaagatgac aatcaacta acaaaaggt atgatcaaa 
      attgttcga aggacggga cagctcaac ttatcggga 
      cgtagtttg gcccatgta cctgaaaaa atgtttact 
      atgtatgaa ttaacagag gaagacact gcgaaatat 
      atagacttc aacaaagag cccgaggag gaaaactgg 
      tggcagttt aaaccctta aattacttg gacctaagc 
      tcaaacgta ctgcaagag attcccgga aaaattgga 
      atgtttgag gacttgact gcactcaat ttgcaagat 
      aacaacata acaagttta ccccctgaa atttgtaat 
      ctaaccaag ttaacaaaa ctaaacctc agccataac 
      aagattaat ttgttgcca atggaattt tacaagtta 
      gtggagtta caggtcctt tcattagct cataacaac 
      ttggaaaaa atctcgaaa aactttgcc gatttggtt 
      atgctacaa caattggac ttgtctcat aatatttta 
      accaaatta ccaccagga atggggttt ttagtgcga 
      ctaactgaa ataaatctc tcacataac aaactaatt 
      gaattacca cccgatatt gttaactta aggggtctc 
      ttgaaattg gatgttact cacaatgac ttggtttac 
      ttgccaaaa atgggcgaa cttgccaag ttacagttt 
      ttgtatgcc caacacaat aatattgag gaaattcca 
      gattttgag ggctgtaca cacttacag caagtctat 
      tttggcaac aattacata aaagaagtt actaccgat 
      ttttgtgaa aacatgtca aatttaaaa attcttgat 
      ttgcgagac aataaaatc gagcaaatt cccaatgaa 
      atcgcgatg ttgcaacac ttgattcga ctcgattta 
      accaataat gatttgaca gatcttccc aattcattg 
      ggtttgtta gcgcacttg caaaatctt caattggaa 
      ggtaacaag ttgaaaaaa atcagaggt gatattatt 
      aaaggagga acaatgcgc attttgaaa catttgaag 
      gagcagctt gatgatgaa gaccttgag agtgttcct 
      aaagtctcc actatgcaa catgaagct aaaacattt 
      cccgataaa tacactatc aaacattca cgttctttg 
      aatgttaca atgaagcag cttacttca gtacctgat 
      gatgttttc gaggaagcc aaattagca gaagttact 
      attgttgac ttgtgtaaa aataaacta ccttcagtg 
      cctaatggg atacaatta gtggccgaa aatttgacc 
      gaattaaat ttgtctatg aatgctatt agcgaaatt 
      cctgaattt atcagcaat tgtataaaa ctgaaatat 
      ttcgatttg ggcaacaat ttattgtcg gatttgccg 
      gaatgttta agttcattg gttggttta agagaactt 
      gtactaagt aacaataga tttgttcac ataccagat 
      tgtgtgtac agtatggta gggttagaa atactttta 
      gctagtgat aacaaaata actgatatt aatgtggag 
      ggactgaaa aatcttaca agaattgca actttagat 
      ttgaccaac aacaacatg agtcatata ccacctgaa 
      ctggggaat gtcactcaa ctcaggaca ctggagtta 
      cgaggtaat tgctttaga caaccgagg tacgcaatt 
      ttagaacaa gggactgct tccgtacta agttatttg 
      agggataga attcctaaa tag
    mRNA sequence
    • >TC008530 RA
      tcggaaatg gcgttctaa taagaaaat tgatacaac 
      ttgcggtat aaaagtccc ggtataaaa gttcatcat 
      tatggagca aatattcta tgctaagat caaccatca 
      atctacgag agggaattg acattagta aatttccaa 
      gcgtcatac cttatttga aacgaaaag gatagaggt 
      catttcaag gttcatgat gtcacaaca aaaacgaaa 
      ggttatgtt tctgtttga ttaatcgat atttttccg 
      ttaaagata ttccacaat ggccaaaca atcaagcgt 
      taaatctaa acgtaaact aataaatcc agtttttca 
      tctacaaac aaaagaaga agatgacaa tcaactaac 
      aaaaggtat gatcaaaat tgttcgaag gacgggaca 
      gctcaactt atcgggacg tagtttggc ccatgtacc 
      tgaaaaaat gtttactat gtatgaatt aacagagga 
      agacactgc gaaatatat agacttcaa caaagagcc 
      cgaggagga aaactggtg gcagtttaa acccttaaa 
      ttacttgga cctaagctc aaacgtact gcaagagat 
      tcccggaaa aattggaat gtttgagga cttgactgc 
      actcaattt gcaagataa caacataac aagtttacc 
      ccctgaaat ttgtaatct aaccaagtt aacaaaact 
      aaacctcag ccataacaa gattaattt gttgccaat 
      ggaatttta caagttagt ggagttaca ggtcctttc 
      attagctca taacaactt ggaaaaaat ctcgaaaaa 
      ctttgccga tttggttat gctacaaca attggactt 
      gtctcataa tattttaac caaattacc accaggaat 
      ggggttttt agtgcgact aactgaaat aaatctctc 
      acataacaa actaattga attaccacc cgatattgt 
      taacttaag gggtctctt gaaattgga tgttactca 
      caatgactt ggtttactt gccaaaaat gggcgaact 
      tgccaagtt acagttttt gtatgccca acacaataa 
      tattgagga aattccaga ttttgaggg ctgtacaca 
      cttacagca agtctattt tggcaacaa ttacataaa 
      agaagttac taccgattt ttgtgaaaa catgtcaaa 
      tttaaaaat tcttgattt gcgagacaa taaaatcga 
      gcaaattcc caatgaaat cgcgatgtt gcaacactt 
      gattcgact cgatttaac caataatga tttgacaga 
      tcttcccaa ttcattggg tttgttagc gcacttgca 
      aaatcttca attggaagg taacaagtt gaaaaaaat 
      cagaggtga tattattaa aggaggaac aatgcgcat 
      tttgaaaca tttgaagga gcagcttga tgatgaaga 
      ccttgagag tgttcctaa agtctccac tatgcaaca 
      tgaagctaa aacatttcc cgataaata cactatcaa 
      acattcacg ttctttgaa tgttacaat gaagcagct 
      tacttcagt acctgatga tgttttcga ggaagccaa 
      attagcaga agttactat tgttgactt gtgtaaaaa 
      taaactacc ttcagtgcc taatgggat acaattagt 
      ggccgaaaa tttgaccga attaaattt gtctatgaa 
      tgctattag cgaaattcc tgaatttat cagcaattg 
      tataaaact gaaatattt cgatttggg caacaattt 
      attgtcgga tttgccgga atgtttaag ttcattggt 
      tggtttaag agaacttgt actaagtaa caatagatt 
      tgttcacat accagattg tgtgtacag tatggtagg 
      gttagaaat acttttagc tagtgataa caaaataac 
      tgatattaa tgtggaggg actgaaaaa tcttacaag 
      aattgcaac tttagattt gaccaacaa caacatgag 
      tcatatacc acctgaact ggggaatgt cactcaact 
      caggacact ggagttacg aggtaattg ctttagaca 
      accgaggta cgcaatttt agaacaagg gactgcttc 
      cgtactaag ttatttgag ggatagaat tcctaaata 
      gtttctagt catagtttt attttctgt tttatttcg 
      taggccttg atcgaaacg tgtgattag caggatcaa 
      attttgata tgattgccc ttttaacca tttgcaaat 
      gttacaggg atatgcatt actttgaaa atgtaatcg 
      aaaatggtt tatttgttt ttattgtat ttcttttcg 
      tttttcttc ctctgtttt ccattttct gtgtatgag 
      ataggcctg ctttagaaa attgttcct tcatttctt 
      acttgcact gttccgaac actttttag gtttgatag 
      aacaaagat agaagtttt ttttatata tcgtcggat 
      gttgcaaaa ataagataa ctcaatttt gttgtcgct 
      aacagtgaa acttatgaa ggcatgtgc ataaaatag 
      catgggtaa ttttttgca ataaccgat ttttaactg 
      ttgtgtaaa agtttaata gttgtcttc tccgaatta 
      ctgcttttt gcaataacg aaacatata gggtgttcc 
      atctaaacc ccacattag aaaattgca agttttaat 
      aattaagta tattcatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY