| General information | |||
| TC008530 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451492.1: 293002..311387 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008530 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01362 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC008530 RA
MAKQSSVKS KRKLINPVF HLQTKEEDD NQLTKGMIK IVRRTGQLN LSGRSLAHV PEKMFTMYE LTEEDTAKY IDFNKEPEE ENWWQFKPL NYLDLSSNV LQEIPGKIG MFEDLTALN LQDNNITSL PPEICNLTK LTKLNLSHN KINLLPMEF YKLVELQVL SLAHNNLEK ISKNFADLV MLQQLDLSH NILTKLPPG MGFLVRLTE INLSHNKLI ELPPDIVNL RGLLKLDVT HNDLVYLPK MGELAKLQF LYAQHNNIE EIPDFEGCT HLQQVYFGN NYIKEVTTD FCENMSNLK ILDLRDNKI EQIPNEIAM LQHLIRLDL TNNDLTDLP NSLGLLAHL QNLQLEGNK LKKIRGDII KGGTMRILK HLKEQLDDE DLESVPKVS TMQHEAKTF PDKYTIKHS RSLNVTMKQ LTSVPDDVF EEAKLAEVT IVDLCKNKL PSVPNGIQL VAENLTELN LSMNAISEI PEFISNCIK LKYFDLGNN LLSDLPECL SSLVGLREL VLSNNRFVH IPDCVYSMV GLEILLASD NKITDINVE GLKNLTRIA TLDLTNNNM SHIPPELGN VTQLRTLEL RGNCFRQPR YAILEQGTA SVLSYLRDR IPK
- >TC008530 RA
atggccaaa caatcaagc gttaaatct aaacgtaaa ctaataaat ccagttttt catctacaa acaaaagaa gaagatgac aatcaacta acaaaaggt atgatcaaa attgttcga aggacggga cagctcaac ttatcggga cgtagtttg gcccatgta cctgaaaaa atgtttact atgtatgaa ttaacagag gaagacact gcgaaatat atagacttc aacaaagag cccgaggag gaaaactgg tggcagttt aaaccctta aattacttg gacctaagc tcaaacgta ctgcaagag attcccgga aaaattgga atgtttgag gacttgact gcactcaat ttgcaagat aacaacata acaagttta ccccctgaa atttgtaat ctaaccaag ttaacaaaa ctaaacctc agccataac aagattaat ttgttgcca atggaattt tacaagtta gtggagtta caggtcctt tcattagct cataacaac ttggaaaaa atctcgaaa aactttgcc gatttggtt atgctacaa caattggac ttgtctcat aatatttta accaaatta ccaccagga atggggttt ttagtgcga ctaactgaa ataaatctc tcacataac aaactaatt gaattacca cccgatatt gttaactta aggggtctc ttgaaattg gatgttact cacaatgac ttggtttac ttgccaaaa atgggcgaa cttgccaag ttacagttt ttgtatgcc caacacaat aatattgag gaaattcca gattttgag ggctgtaca cacttacag caagtctat tttggcaac aattacata aaagaagtt actaccgat ttttgtgaa aacatgtca aatttaaaa attcttgat ttgcgagac aataaaatc gagcaaatt cccaatgaa atcgcgatg ttgcaacac ttgattcga ctcgattta accaataat gatttgaca gatcttccc aattcattg ggtttgtta gcgcacttg caaaatctt caattggaa ggtaacaag ttgaaaaaa atcagaggt gatattatt aaaggagga acaatgcgc attttgaaa catttgaag gagcagctt gatgatgaa gaccttgag agtgttcct aaagtctcc actatgcaa catgaagct aaaacattt cccgataaa tacactatc aaacattca cgttctttg aatgttaca atgaagcag cttacttca gtacctgat gatgttttc gaggaagcc aaattagca gaagttact attgttgac ttgtgtaaa aataaacta ccttcagtg cctaatggg atacaatta gtggccgaa aatttgacc gaattaaat ttgtctatg aatgctatt agcgaaatt cctgaattt atcagcaat tgtataaaa ctgaaatat ttcgatttg ggcaacaat ttattgtcg gatttgccg gaatgttta agttcattg gttggttta agagaactt gtactaagt aacaataga tttgttcac ataccagat tgtgtgtac agtatggta gggttagaa atactttta gctagtgat aacaaaata actgatatt aatgtggag ggactgaaa aatcttaca agaattgca actttagat ttgaccaac aacaacatg agtcatata ccacctgaa ctggggaat gtcactcaa ctcaggaca ctggagtta cgaggtaat tgctttaga caaccgagg tacgcaatt ttagaacaa gggactgct tccgtacta agttatttg agggataga attcctaaa tag
- >TC008530 RA
tcggaaatg gcgttctaa taagaaaat tgatacaac ttgcggtat aaaagtccc ggtataaaa gttcatcat tatggagca aatattcta tgctaagat caaccatca atctacgag agggaattg acattagta aatttccaa gcgtcatac cttatttga aacgaaaag gatagaggt catttcaag gttcatgat gtcacaaca aaaacgaaa ggttatgtt tctgtttga ttaatcgat atttttccg ttaaagata ttccacaat ggccaaaca atcaagcgt taaatctaa acgtaaact aataaatcc agtttttca tctacaaac aaaagaaga agatgacaa tcaactaac aaaaggtat gatcaaaat tgttcgaag gacgggaca gctcaactt atcgggacg tagtttggc ccatgtacc tgaaaaaat gtttactat gtatgaatt aacagagga agacactgc gaaatatat agacttcaa caaagagcc cgaggagga aaactggtg gcagtttaa acccttaaa ttacttgga cctaagctc aaacgtact gcaagagat tcccggaaa aattggaat gtttgagga cttgactgc actcaattt gcaagataa caacataac aagtttacc ccctgaaat ttgtaatct aaccaagtt aacaaaact aaacctcag ccataacaa gattaattt gttgccaat ggaatttta caagttagt ggagttaca ggtcctttc attagctca taacaactt ggaaaaaat ctcgaaaaa ctttgccga tttggttat gctacaaca attggactt gtctcataa tattttaac caaattacc accaggaat ggggttttt agtgcgact aactgaaat aaatctctc acataacaa actaattga attaccacc cgatattgt taacttaag gggtctctt gaaattgga tgttactca caatgactt ggtttactt gccaaaaat gggcgaact tgccaagtt acagttttt gtatgccca acacaataa tattgagga aattccaga ttttgaggg ctgtacaca cttacagca agtctattt tggcaacaa ttacataaa agaagttac taccgattt ttgtgaaaa catgtcaaa tttaaaaat tcttgattt gcgagacaa taaaatcga gcaaattcc caatgaaat cgcgatgtt gcaacactt gattcgact cgatttaac caataatga tttgacaga tcttcccaa ttcattggg tttgttagc gcacttgca aaatcttca attggaagg taacaagtt gaaaaaaat cagaggtga tattattaa aggaggaac aatgcgcat tttgaaaca tttgaagga gcagcttga tgatgaaga ccttgagag tgttcctaa agtctccac tatgcaaca tgaagctaa aacatttcc cgataaata cactatcaa acattcacg ttctttgaa tgttacaat gaagcagct tacttcagt acctgatga tgttttcga ggaagccaa attagcaga agttactat tgttgactt gtgtaaaaa taaactacc ttcagtgcc taatgggat acaattagt ggccgaaaa tttgaccga attaaattt gtctatgaa tgctattag cgaaattcc tgaatttat cagcaattg tataaaact gaaatattt cgatttggg caacaattt attgtcgga tttgccgga atgtttaag ttcattggt tggtttaag agaacttgt actaagtaa caatagatt tgttcacat accagattg tgtgtacag tatggtagg gttagaaat acttttagc tagtgataa caaaataac tgatattaa tgtggaggg actgaaaaa tcttacaag aattgcaac tttagattt gaccaacaa caacatgag tcatatacc acctgaact ggggaatgt cactcaact caggacact ggagttacg aggtaattg ctttagaca accgaggta cgcaatttt agaacaagg gactgcttc cgtactaag ttatttgag ggatagaat tcctaaata gtttctagt catagtttt attttctgt tttatttcg taggccttg atcgaaacg tgtgattag caggatcaa attttgata tgattgccc ttttaacca tttgcaaat gttacaggg atatgcatt actttgaaa atgtaatcg aaaatggtt tatttgttt ttattgtat ttcttttcg tttttcttc ctctgtttt ccattttct gtgtatgag ataggcctg ctttagaaa attgttcct tcatttctt acttgcact gttccgaac actttttag gtttgatag aacaaagat agaagtttt ttttatata tcgtcggat gttgcaaaa ataagataa ctcaatttt gttgtcgct aacagtgaa acttatgaa ggcatgtgc ataaaatag catgggtaa ttttttgca ataaccgat ttttaactg ttgtgtaaa agtttaata gttgtcttc tccgaatta ctgcttttt gcaataacg aaacatata gggtgttcc atctaaacc ccacattag aaaattgca agttttaat aattaagta tattcatc
