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Gene information
General information
TC008514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11989494..12009173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008514
    iBeetle screen iB_01361

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008514 RA
      MVEDTSSED NTTTNKGVN ARSESLPVR VHRFSESET 
      SSSNNAPRG SISDIKNEI QNVLDLMGP TTPDLTTSH 
      YTSSDAEND ESVLSALDT RRTRLSSGC DKCSNNCCS 
      SSKSSNKKD SVMADAGTC SLNNFEHFE DIEPSCCDH 
      NHFSVRPEC LCMRPVASS TRCEGYYLM PNKSGSKLT 
      TVDCKKRNL FEWIKNRSH ELIIRKQNY SPCDLYKIN 
      ICGQEDDTG PPKRVVFDL VDGIPRPSS TTVATNTIH 
      NPVEKTTKN NETNSNNNC NCSNEIKPK RTMTMAQST 
      IQKNVYYDS EGNTRVDYY YFDHGNAQF LHTTDTPPV 
      MKCELIAEK TEKYTTKFW AEFFASIHI GVSFCVSFI 
      LQLFKFLLY SVFRPIFVG LMQMWSDYF FKPFLTTLF 
      NGVIQPFLI FLYNIATSV RDLCDPIAE AFGYYCREL 
      AVLLKAIRL CSYKKNGKE DSSCDYRKK YKITTSNHS 
      QLIQRTTNI TLVNTNMLR ITGGTILKT KKIPKLQIH 
      KYSTENQLP NQADVVVIG GGVAGCSLL YNLSKRGVK 
      AVLLETAKI TSGTTWHTS ALCWRLRPC DVDIKLLES 
      TRKLLIDLE QETGLNTGW INNGGLFIA KTEERIQEY 
      KRLHTFGHF FNIESHIIS PQEASKLSP MLDPNKFKG 
      ALYSPGDGF TDPSMYCAA LIKGATTKG GQVIENCSV 
      TNILTDQST NVRKITGVE TSKGTIKTS CVVNAGGVW 
      SKNIADLVG LDLPVVPMK HSYIITESI PEVKNTPNI 
      RDHDGSIYL RIQGDSVYL GGYENNPEI IRDMPSDFQ 
      FSLFELDKS LFEVHLKSA MDIAPVFGN IGIKSDVCG 
      AEAFTPDHK PIMGEDPRL AGYFYSCGY NSLGINLSG 
      GCAEQLGIW ITKGRPEEP LFAYDIRRF TPNQRSDRA 
      WVTETSQEC YAKNYSIVF PHDQPLAGR NHKIGPFHE 
      ALVAGGAVM EQAQGWERP GYFVKDRTA PVRGYDWYG 
      YYDHVDNPD KRYEQELEG DYTFGFSKH HDLIGEEAN 
      AARNNVALF DLSYFTKMY LTGPDAEKA ADWIFTADT 
      DKEPGRVVY TCSLNSRGG VEADVTVTA LEEGVGTLV 
      GPILKGKGY YIVAGGASG YQTVSHFKK ELAKKNFKA 
      VISDITDKL GVLSIQGPK SRELLQSIT ETPITDEKF 
      PPGMSHLIK VNGHVCRAM RVSFIGELG YELHVPVAS 
      CVAVFNKVV DAGRGFDLR HAGFRALYS LSLEKGYHL 
      WNHDLRIDD NPVEANLQG ICRKDGQYL GKQHVEKLK 
      EEGVKKRRV FFTLEDQVA LYGLETIWR DDTIVGYLR 
      RGDYGFNLD CSIGTGYIE HPKGKIVTD EFLKSGNYQ 
      IEVLDKRYP ATVFLKSPF DPKNQRLLG QYDQQFEEQ 
      SHFED
    CDS sequence
    • >TC008514 RA
      atggtggaa gacacaagt agtgaagat aacacaaca 
      actaacaaa ggtgtgaat gcaagatct gaatctttg 
      ccagttcga gttcacaga ttttcggaa agtgaaact 
      tcgtcgtca aacaatgca ccaagaggt tccatatcc 
      gacataaaa aacgaaatt caaaacgta ttagatcta 
      atgggtcca acaactccc gatttgacc acatcacat 
      tacacaagt agtgatgca gaaaacgat gagtctgtt 
      ttgtcagca cttgataca cgtcgcacg agactttct 
      tcgggttgt gacaagtgt tcaaacaat tgttgttct 
      tcgagtaag agttcaaat aaaaaggat tcggtaatg 
      gcagatgct gggacctgt tcattaaac aattttgag 
      cactttgaa gatattgaa ccttcatgt tgtgatcat 
      aatcatttt agtgtgagg cctgagtgc ttgtgtatg 
      agacctgtt gcttcgtcc acaagatgt gaaggttac 
      taccttatg cccaacaaa agtgggtcg aaattaaca 
      acagttgac tgtaaaaaa cgaaattta ttcgaatgg 
      ataaaaaat agatcacac gaacttata ataaggaaa 
      caaaactac agtccttgt gacttgtat aaaattaat 
      atttgtgga caagaagac gacacaggt cctccaaaa 
      cgagtagtt ttcgatttg gttgatggt atccctcgg 
      ccatcttcg accactgtt gcaacaaac acaatccat 
      aatcctgtc gaaaaaaca actaaaaac aacgaaaca 
      aattcgaac aataattgt aactgtagt aacgaaata 
      aaaccgaaa agaacaatg acaatggca caaagtacg 
      attcaaaaa aatgtttac tacgacagt gaaggaaat 
      acaagagtt gattattat tattttgac cacggcaat 
      gcgcagttt ctgcatacg actgatacg cctcctgtg 
      atgaaatgc gaacttata gctgaaaaa actgaaaaa 
      tatacgaca aaattttgg gccgagttt tttgcttca 
      attcacatt ggagtgtct ttttgtgtg tcttttatt 
      ctacagttg tttaaattt ttgttgtat agtgtgttt 
      cgtccaatc tttgtcggt ttaatgcag atgtggtcg 
      gattatttc ttcaaacct tttcttaca acgctgttt 
      aatggagtg attcaaccg tttttaatt tttctttac 
      aatattgct acttcggtt agggatttg tgtgatccg 
      attgcggaa gcttttggg tattattgt cgtgaactg 
      gcggttttg cttaaggct attcggttg tgtagttat 
      aagaagaat ggcaaagaa gatagtagt tgtgattac 
      aggaaaaaa tataaaata accacatcc aatcacagt 
      caattaatc cagcgtacg acgaacatc actcttgta 
      aacacaaac atgctgcga ataaccggc ggaacgatt 
      ctcaagacg aaaaaaata cctaaacta caaattcac 
      aaatactcc accgaaaat cagttgccg aaccaagca 
      gatgttgtg gttatcggg ggtggcgtg gccggttgt 
      agtctcctc tacaactta tccaaaaga ggcgttaag 
      gccgttttg ctagaaaca gccaaaatc acaagtggc 
      accacttgg cacacaagc gctctgtgt tggaggcta 
      agaccttgt gatgttgat atcaaattg ttggaatct 
      accaggaaa cttctcatt gatttggaa caagaaacg 
      ggacttaat acaggatgg attaataat ggggggctt 
      ttcattgct aaaacagag gaacgaatc caagaatac 
      aaacgtctt cacaccttc ggccacttt ttcaacatt 
      gaaagccat ataatctca cctcaagaa gcttcaaaa 
      ctctcacca atgctcgac cctaacaag ttcaaagga 
      gccctttat tcacctggc gatggcttt actgaccct 
      tccatgtac tgtgcagcc cttataaaa ggcgccact 
      acaaaaggg gggcaagta atcgaaaat tgttcagtt 
      acaaatatc ctaactgac caatcaaca aacgtgcga 
      aaaatcaca ggcgtggaa acatcgaaa ggtaccatc 
      aaaactagc tgtgttgtg aacgctggt ggagtttgg 
      tcgaaaaat attgccgat ctagtaggt ctagaccta 
      ccagtggta ccaatgaaa cattcatac atcatcacc 
      gaaagtata cctgaagtg aaaaacaca ccaaatatt 
      cgtgaccac gatgggtcc atttatctc cgaattcag 
      ggtgatagt gtctatcta ggtggctat gaaaacaac 
      ccggaaata atcagagac atgccgtcc gatttccag 
      tttagtttg ttcgaactg gataaaagt ctgtttgag 
      gtgcattta aaaagtgcc atggatata gccccagtt 
      tttggcaat atagggatc aaatcggat gtttgtggg 
      gctgaggcg ttcacacct gaccataaa cccatcatg 
      ggtgaggat cccaggctt gcaggttat ttctactcg 
      tgtgggtac aattcgttg ggtataaac ttgagtggt 
      ggctgtgcc gaacagttg ggcatatgg ataacgaaa 
      ggtcggccc gaagaacca ttatttgcg tatgatata 
      aggagattt acgcccaac caacgatcg gatcgggct 
      tgggttacc gaaactagt caagaatgc tatgcgaaa 
      aattatagt attgttttt ccacatgat caaccttta 
      gctggacga aatcataaa attggaccg tttcatgag 
      gctttagtc gccggcggt gctgtaatg gagcaggcc 
      caaggctgg gaacgtcca ggttacttc gtcaaagac 
      aggacagcc cccgtgcgg ggctacgac tggtacggc 
      tattacgac cacgtcgac aatcccgac aaaagatac 
      gagcaagaa ctggagggc gattacact ttcggcttc 
      tccaaacat cacgatttg attggggag gaagccaat 
      gccgctcga aacaacgtt gcacttttc gacctctcc 
      tacttcacc aaaatgtac ttaacaggt cccgatgct 
      gaaaaagct gccgattgg attttcaca gctgataca 
      gataaagaa ccaggacgt gttgtttat acttgttct 
      ttgaatagt agaggtggg gtcgaggct gatgtgact 
      gttacagca ttggaggaa ggtgtcgga actctagtt 
      gggcccatt ttgaagggg aaaggttac tatattgtg 
      gctggtggc gcatcagga taccaaaca gtctcacac 
      ttcaagaaa gaactagcc aagaagaat ttcaaagct 
      gttattagt gatattacg gataagtta ggagttctt 
      tcaattcag ggacccaaa agtcgggaa cttttacaa 
      tcaattact gaaacacca atcactgat gagaaattc 
      ccacctggg atgtcgcac cttattaaa gtcaatggt 
      cacgtgtgc cgtgccatg agggttagt tttattgga 
      gaattggga tatgaattg cacgttcct gtggcatcg 
      tgtgttgcc gtttttaac aaagttgtg gatgctgga 
      cgagggttt gacttgaga catgccggt ttccgcgcg 
      ttgtattct ctgagtctt gagaaaggt taccacttg 
      tggaatcac gacctgcga attgatgat aatcccgtt 
      gaagcgaat ttgcaaggg atttgtcgg aaggatgga 
      caataccta ggtaaacaa catgtagag aaattgaag 
      gaagaaggg gtgaagaaa cgtagagtt ttcttcaca 
      ttggaggat caagtcgct ctttatggc ttggagacc 
      atttggagg gacgatacc attgtgggg tatttgaga 
      agaggggat tatggattt aacctcgat tgtagtatt 
      ggcactgga tatattgaa catcccaag ggaaaaatt 
      gtgacggat gaattcctc aaaagtgga aattatcag 
      attgaagtt ttggataaa aggtatcca gcgactgtg 
      tttttgaag agtcccttc gatccgaaa aatcagagg 
      ctgttagga cagtacgac caacagttt gaggaacag 
      tcgcatttt gaggattaa
    mRNA sequence
    • >TC008514 RA
      tttcttatc atggatttt acactttta caaacgttt 
      atacaatac aaatattta tgaaatggt ggaagacac 
      aagtagtga agataacac aacaactaa caaaggtgt 
      gaatgcaag atctgaatc tttgccagt tcgagttca 
      cagattttc ggaaagtga aacttcgtc gtcaaacaa 
      tgcaccaag aggttccat atccgacat aaaaaacga 
      aattcaaaa cgtattaga tctaatggg tccaacaac 
      tcccgattt gaccacatc acattacac aagtagtga 
      tgcagaaaa cgatgagtc tgttttgtc agcacttga 
      tacacgtcg cacgagact ttcttcggg ttgtgacaa 
      gtgttcaaa caattgttg ttcttcgag taagagttc 
      aaataaaaa ggattcggt aatggcaga tgctgggac 
      ctgttcatt aaacaattt tgagcactt tgaagatat 
      tgaaccttc atgttgtga tcataatca ttttagtgt 
      gaggcctga gtgcttgtg tatgagacc tgttgcttc 
      gtccacaag atgtgaagg ttactacct tatgcccaa 
      caaaagtgg gtcgaaatt aacaacagt tgactgtaa 
      aaaacgaaa tttattcga atggataaa aaatagatc 
      acacgaact tataataag gaaacaaaa ctacagtcc 
      ttgtgactt gtataaaat taatatttg tggacaaga 
      agacgacac aggtcctcc aaaacgagt agttttcga 
      tttggttga tggtatccc tcggccatc ttcgaccac 
      tgttgcaac aaacacaat ccataatcc tgtcgaaaa 
      aacaactaa aaacaacga aacaaattc gaacaataa 
      ttgtaactg tagtaacga aataaaacc gaaaagaac 
      aatgacaat ggcacaaag tacgattca aaaaaatgt 
      ttactacga cagtgaagg aaatacaag agttgatta 
      ttattattt tgaccacgg caatgcgca gtttctgca 
      tacgactga tacgcctcc tgtgatgaa atgcgaact 
      tatagctga aaaaactga aaaatatac gacaaaatt 
      ttgggccga gttttttgc ttcaattca cattggagt 
      gtctttttg tgtgtcttt tattctaca gttgtttaa 
      atttttgtt gtatagtgt gtttcgtcc aatctttgt 
      cggtttaat gcagatgtg gtcggatta tttcttcaa 
      accttttct tacaacgct gtttaatgg agtgattca 
      accgttttt aatttttct ttacaatat tgctacttc 
      ggttaggga tttgtgtga tccgattgc ggaagcttt 
      tgggtatta ttgtcgtga actggcggt tttgcttaa 
      ggctattcg gttgtgtag ttataagaa gaatggcaa 
      agaagatag tagttgtga ttacaggaa aaaatataa 
      aataaccac atccaatca cagtcaatt aatccagcg 
      tacgacgaa catcactct tgtaaacac aaacatgct 
      gcgaataac cggcggaac gattctcaa gacgaaaaa 
      aatacctaa actacaaat tcacaaata ctccaccga 
      aaatcagtt gccgaacca agcagatgt tgtggttat 
      cgggggtgg cgtggccgg ttgtagtct cctctacaa 
      cttatccaa aagaggcgt taaggccgt tttgctaga 
      aacagccaa aatcacaag tggcaccac ttggcacac 
      aagcgctct gtgttggag gctaagacc ttgtgatgt 
      tgatatcaa attgttgga atctaccag gaaacttct 
      cattgattt ggaacaaga aacgggact taatacagg 
      atggattaa taatggggg gcttttcat tgctaaaac 
      agaggaacg aatccaaga atacaaacg tcttcacac 
      cttcggcca ctttttcaa cattgaaag ccatataat 
      ctcacctca agaagcttc aaaactctc accaatgct 
      cgaccctaa caagttcaa aggagccct ttattcacc 
      tggcgatgg ctttactga cccttccat gtactgtgc 
      agcccttat aaaaggcgc cactacaaa aggggggca 
      agtaatcga aaattgttc agttacaaa tatcctaac 
      tgaccaatc aacaaacgt gcgaaaaat cacaggcgt 
      ggaaacatc gaaaggtac catcaaaac tagctgtgt 
      tgtgaacgc tggtggagt ttggtcgaa aaatattgc 
      cgatctagt aggtctaga cctaccagt ggtaccaat 
      gaaacattc atacatcat caccgaaag tatacctga 
      agtgaaaaa cacaccaaa tattcgtga ccacgatgg 
      gtccattta tctccgaat tcagggtga tagtgtcta 
      tctaggtgg ctatgaaaa caacccgga aataatcag 
      agacatgcc gtccgattt ccagtttag tttgttcga 
      actggataa aagtctgtt tgaggtgca tttaaaaag 
      tgccatgga tatagcccc agtttttgg caatatagg 
      gatcaaatc ggatgtttg tggggctga ggcgttcac 
      acctgacca taaacccat catgggtga ggatcccag 
      gcttgcagg ttatttcta ctcgtgtgg gtacaattc 
      gttgggtat aaacttgag tggtggctg tgccgaaca 
      gttgggcat atggataac gaaaggtcg gcccgaaga 
      accattatt tgcgtatga tataaggag atttacgcc 
      caaccaacg atcggatcg ggcttgggt taccgaaac 
      tagtcaaga atgctatgc gaaaaatta tagtattgt 
      ttttccaca tgatcaacc tttagctgg acgaaatca 
      taaaattgg accgtttca tgaggcttt agtcgccgg 
      cggtgctgt aatggagca ggcccaagg ctgggaacg 
      tccaggtta cttcgtcaa agacaggac agcccccgt 
      gcggggcta cgactggta cggctatta cgaccacgt 
      cgacaatcc cgacaaaag atacgagca agaactgga 
      gggcgatta cactttcgg cttctccaa acatcacga 
      tttgattgg ggaggaagc caatgccgc tcgaaacaa 
      cgttgcact tttcgacct ctcctactt caccaaaat 
      gtacttaac aggtcccga tgctgaaaa agctgccga 
      ttggatttt cacagctga tacagataa agaaccagg 
      acgtgttgt ttatacttg ttctttgaa tagtagagg 
      tggggtcga ggctgatgt gactgttac agcattgga 
      ggaaggtgt cggaactct agttgggcc cattttgaa 
      ggggaaagg ttactatat tgtggctgg tggcgcatc 
      aggatacca aacagtctc acacttcaa gaaagaact 
      agccaagaa gaatttcaa agctgttat tagtgatat 
      tacggataa gttaggagt tctttcaat tcagggacc 
      caaaagtcg ggaactttt acaatcaat tactgaaac 
      accaatcac tgatgagaa attcccacc tgggatgtc 
      gcaccttat taaagtcaa tggtcacgt gtgccgtgc 
      catgagggt tagttttat tggagaatt gggatatga 
      attgcacgt tcctgtggc atcgtgtgt tgccgtttt 
      taacaaagt tgtggatgc tggacgagg gtttgactt 
      gagacatgc cggtttccg cgcgttgta ttctctgag 
      tcttgagaa aggttacca cttgtggaa tcacgacct 
      gcgaattga tgataatcc cgttgaagc gaatttgca 
      agggatttg tcggaagga tggacaata cctaggtaa 
      acaacatgt agagaaatt gaaggaaga aggggtgaa 
      gaaacgtag agttttctt cacattgga ggatcaagt 
      cgctcttta tggcttgga gaccatttg gagggacga 
      taccattgt ggggtattt gagaagagg ggattatgg 
      atttaacct cgattgtag tattggcac tggatatat 
      tgaacatcc caagggaaa aattgtgac ggatgaatt 
      cctcaaaag tggaaatta tcagattga agttttgga 
      taaaaggta tccagcgac tgtgttttt gaagagtcc 
      cttcgatcc gaaaaatca gaggctgtt aggacagta 
      cgaccaaca gtttgagga acagtcgca ttttgagga 
      ttaatattg ttgttgttt tataatttt taattgtaa 
      taaaaaata aaaaccata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY