| General information | |||
| TC008514 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 11989494..12009173 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008514 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01361 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
- >TC008514 RA
MVEDTSSED NTTTNKGVN ARSESLPVR VHRFSESET SSSNNAPRG SISDIKNEI QNVLDLMGP TTPDLTTSH YTSSDAEND ESVLSALDT RRTRLSSGC DKCSNNCCS SSKSSNKKD SVMADAGTC SLNNFEHFE DIEPSCCDH NHFSVRPEC LCMRPVASS TRCEGYYLM PNKSGSKLT TVDCKKRNL FEWIKNRSH ELIIRKQNY SPCDLYKIN ICGQEDDTG PPKRVVFDL VDGIPRPSS TTVATNTIH NPVEKTTKN NETNSNNNC NCSNEIKPK RTMTMAQST IQKNVYYDS EGNTRVDYY YFDHGNAQF LHTTDTPPV MKCELIAEK TEKYTTKFW AEFFASIHI GVSFCVSFI LQLFKFLLY SVFRPIFVG LMQMWSDYF FKPFLTTLF NGVIQPFLI FLYNIATSV RDLCDPIAE AFGYYCREL AVLLKAIRL CSYKKNGKE DSSCDYRKK YKITTSNHS QLIQRTTNI TLVNTNMLR ITGGTILKT KKIPKLQIH KYSTENQLP NQADVVVIG GGVAGCSLL YNLSKRGVK AVLLETAKI TSGTTWHTS ALCWRLRPC DVDIKLLES TRKLLIDLE QETGLNTGW INNGGLFIA KTEERIQEY KRLHTFGHF FNIESHIIS PQEASKLSP MLDPNKFKG ALYSPGDGF TDPSMYCAA LIKGATTKG GQVIENCSV TNILTDQST NVRKITGVE TSKGTIKTS CVVNAGGVW SKNIADLVG LDLPVVPMK HSYIITESI PEVKNTPNI RDHDGSIYL RIQGDSVYL GGYENNPEI IRDMPSDFQ FSLFELDKS LFEVHLKSA MDIAPVFGN IGIKSDVCG AEAFTPDHK PIMGEDPRL AGYFYSCGY NSLGINLSG GCAEQLGIW ITKGRPEEP LFAYDIRRF TPNQRSDRA WVTETSQEC YAKNYSIVF PHDQPLAGR NHKIGPFHE ALVAGGAVM EQAQGWERP GYFVKDRTA PVRGYDWYG YYDHVDNPD KRYEQELEG DYTFGFSKH HDLIGEEAN AARNNVALF DLSYFTKMY LTGPDAEKA ADWIFTADT DKEPGRVVY TCSLNSRGG VEADVTVTA LEEGVGTLV GPILKGKGY YIVAGGASG YQTVSHFKK ELAKKNFKA VISDITDKL GVLSIQGPK SRELLQSIT ETPITDEKF PPGMSHLIK VNGHVCRAM RVSFIGELG YELHVPVAS CVAVFNKVV DAGRGFDLR HAGFRALYS LSLEKGYHL WNHDLRIDD NPVEANLQG ICRKDGQYL GKQHVEKLK EEGVKKRRV FFTLEDQVA LYGLETIWR DDTIVGYLR RGDYGFNLD CSIGTGYIE HPKGKIVTD EFLKSGNYQ IEVLDKRYP ATVFLKSPF DPKNQRLLG QYDQQFEEQ SHFED
- >TC008514 RA
atggtggaa gacacaagt agtgaagat aacacaaca actaacaaa ggtgtgaat gcaagatct gaatctttg ccagttcga gttcacaga ttttcggaa agtgaaact tcgtcgtca aacaatgca ccaagaggt tccatatcc gacataaaa aacgaaatt caaaacgta ttagatcta atgggtcca acaactccc gatttgacc acatcacat tacacaagt agtgatgca gaaaacgat gagtctgtt ttgtcagca cttgataca cgtcgcacg agactttct tcgggttgt gacaagtgt tcaaacaat tgttgttct tcgagtaag agttcaaat aaaaaggat tcggtaatg gcagatgct gggacctgt tcattaaac aattttgag cactttgaa gatattgaa ccttcatgt tgtgatcat aatcatttt agtgtgagg cctgagtgc ttgtgtatg agacctgtt gcttcgtcc acaagatgt gaaggttac taccttatg cccaacaaa agtgggtcg aaattaaca acagttgac tgtaaaaaa cgaaattta ttcgaatgg ataaaaaat agatcacac gaacttata ataaggaaa caaaactac agtccttgt gacttgtat aaaattaat atttgtgga caagaagac gacacaggt cctccaaaa cgagtagtt ttcgatttg gttgatggt atccctcgg ccatcttcg accactgtt gcaacaaac acaatccat aatcctgtc gaaaaaaca actaaaaac aacgaaaca aattcgaac aataattgt aactgtagt aacgaaata aaaccgaaa agaacaatg acaatggca caaagtacg attcaaaaa aatgtttac tacgacagt gaaggaaat acaagagtt gattattat tattttgac cacggcaat gcgcagttt ctgcatacg actgatacg cctcctgtg atgaaatgc gaacttata gctgaaaaa actgaaaaa tatacgaca aaattttgg gccgagttt tttgcttca attcacatt ggagtgtct ttttgtgtg tcttttatt ctacagttg tttaaattt ttgttgtat agtgtgttt cgtccaatc tttgtcggt ttaatgcag atgtggtcg gattatttc ttcaaacct tttcttaca acgctgttt aatggagtg attcaaccg tttttaatt tttctttac aatattgct acttcggtt agggatttg tgtgatccg attgcggaa gcttttggg tattattgt cgtgaactg gcggttttg cttaaggct attcggttg tgtagttat aagaagaat ggcaaagaa gatagtagt tgtgattac aggaaaaaa tataaaata accacatcc aatcacagt caattaatc cagcgtacg acgaacatc actcttgta aacacaaac atgctgcga ataaccggc ggaacgatt ctcaagacg aaaaaaata cctaaacta caaattcac aaatactcc accgaaaat cagttgccg aaccaagca gatgttgtg gttatcggg ggtggcgtg gccggttgt agtctcctc tacaactta tccaaaaga ggcgttaag gccgttttg ctagaaaca gccaaaatc acaagtggc accacttgg cacacaagc gctctgtgt tggaggcta agaccttgt gatgttgat atcaaattg ttggaatct accaggaaa cttctcatt gatttggaa caagaaacg ggacttaat acaggatgg attaataat ggggggctt ttcattgct aaaacagag gaacgaatc caagaatac aaacgtctt cacaccttc ggccacttt ttcaacatt gaaagccat ataatctca cctcaagaa gcttcaaaa ctctcacca atgctcgac cctaacaag ttcaaagga gccctttat tcacctggc gatggcttt actgaccct tccatgtac tgtgcagcc cttataaaa ggcgccact acaaaaggg gggcaagta atcgaaaat tgttcagtt acaaatatc ctaactgac caatcaaca aacgtgcga aaaatcaca ggcgtggaa acatcgaaa ggtaccatc aaaactagc tgtgttgtg aacgctggt ggagtttgg tcgaaaaat attgccgat ctagtaggt ctagaccta ccagtggta ccaatgaaa cattcatac atcatcacc gaaagtata cctgaagtg aaaaacaca ccaaatatt cgtgaccac gatgggtcc atttatctc cgaattcag ggtgatagt gtctatcta ggtggctat gaaaacaac ccggaaata atcagagac atgccgtcc gatttccag tttagtttg ttcgaactg gataaaagt ctgtttgag gtgcattta aaaagtgcc atggatata gccccagtt tttggcaat atagggatc aaatcggat gtttgtggg gctgaggcg ttcacacct gaccataaa cccatcatg ggtgaggat cccaggctt gcaggttat ttctactcg tgtgggtac aattcgttg ggtataaac ttgagtggt ggctgtgcc gaacagttg ggcatatgg ataacgaaa ggtcggccc gaagaacca ttatttgcg tatgatata aggagattt acgcccaac caacgatcg gatcgggct tgggttacc gaaactagt caagaatgc tatgcgaaa aattatagt attgttttt ccacatgat caaccttta gctggacga aatcataaa attggaccg tttcatgag gctttagtc gccggcggt gctgtaatg gagcaggcc caaggctgg gaacgtcca ggttacttc gtcaaagac aggacagcc cccgtgcgg ggctacgac tggtacggc tattacgac cacgtcgac aatcccgac aaaagatac gagcaagaa ctggagggc gattacact ttcggcttc tccaaacat cacgatttg attggggag gaagccaat gccgctcga aacaacgtt gcacttttc gacctctcc tacttcacc aaaatgtac ttaacaggt cccgatgct gaaaaagct gccgattgg attttcaca gctgataca gataaagaa ccaggacgt gttgtttat acttgttct ttgaatagt agaggtggg gtcgaggct gatgtgact gttacagca ttggaggaa ggtgtcgga actctagtt gggcccatt ttgaagggg aaaggttac tatattgtg gctggtggc gcatcagga taccaaaca gtctcacac ttcaagaaa gaactagcc aagaagaat ttcaaagct gttattagt gatattacg gataagtta ggagttctt tcaattcag ggacccaaa agtcgggaa cttttacaa tcaattact gaaacacca atcactgat gagaaattc ccacctggg atgtcgcac cttattaaa gtcaatggt cacgtgtgc cgtgccatg agggttagt tttattgga gaattggga tatgaattg cacgttcct gtggcatcg tgtgttgcc gtttttaac aaagttgtg gatgctgga cgagggttt gacttgaga catgccggt ttccgcgcg ttgtattct ctgagtctt gagaaaggt taccacttg tggaatcac gacctgcga attgatgat aatcccgtt gaagcgaat ttgcaaggg atttgtcgg aaggatgga caataccta ggtaaacaa catgtagag aaattgaag gaagaaggg gtgaagaaa cgtagagtt ttcttcaca ttggaggat caagtcgct ctttatggc ttggagacc atttggagg gacgatacc attgtgggg tatttgaga agaggggat tatggattt aacctcgat tgtagtatt ggcactgga tatattgaa catcccaag ggaaaaatt gtgacggat gaattcctc aaaagtgga aattatcag attgaagtt ttggataaa aggtatcca gcgactgtg tttttgaag agtcccttc gatccgaaa aatcagagg ctgttagga cagtacgac caacagttt gaggaacag tcgcatttt gaggattaa
- >TC008514 RA
tttcttatc atggatttt acactttta caaacgttt atacaatac aaatattta tgaaatggt ggaagacac aagtagtga agataacac aacaactaa caaaggtgt gaatgcaag atctgaatc tttgccagt tcgagttca cagattttc ggaaagtga aacttcgtc gtcaaacaa tgcaccaag aggttccat atccgacat aaaaaacga aattcaaaa cgtattaga tctaatggg tccaacaac tcccgattt gaccacatc acattacac aagtagtga tgcagaaaa cgatgagtc tgttttgtc agcacttga tacacgtcg cacgagact ttcttcggg ttgtgacaa gtgttcaaa caattgttg ttcttcgag taagagttc aaataaaaa ggattcggt aatggcaga tgctgggac ctgttcatt aaacaattt tgagcactt tgaagatat tgaaccttc atgttgtga tcataatca ttttagtgt gaggcctga gtgcttgtg tatgagacc tgttgcttc gtccacaag atgtgaagg ttactacct tatgcccaa caaaagtgg gtcgaaatt aacaacagt tgactgtaa aaaacgaaa tttattcga atggataaa aaatagatc acacgaact tataataag gaaacaaaa ctacagtcc ttgtgactt gtataaaat taatatttg tggacaaga agacgacac aggtcctcc aaaacgagt agttttcga tttggttga tggtatccc tcggccatc ttcgaccac tgttgcaac aaacacaat ccataatcc tgtcgaaaa aacaactaa aaacaacga aacaaattc gaacaataa ttgtaactg tagtaacga aataaaacc gaaaagaac aatgacaat ggcacaaag tacgattca aaaaaatgt ttactacga cagtgaagg aaatacaag agttgatta ttattattt tgaccacgg caatgcgca gtttctgca tacgactga tacgcctcc tgtgatgaa atgcgaact tatagctga aaaaactga aaaatatac gacaaaatt ttgggccga gttttttgc ttcaattca cattggagt gtctttttg tgtgtcttt tattctaca gttgtttaa atttttgtt gtatagtgt gtttcgtcc aatctttgt cggtttaat gcagatgtg gtcggatta tttcttcaa accttttct tacaacgct gtttaatgg agtgattca accgttttt aatttttct ttacaatat tgctacttc ggttaggga tttgtgtga tccgattgc ggaagcttt tgggtatta ttgtcgtga actggcggt tttgcttaa ggctattcg gttgtgtag ttataagaa gaatggcaa agaagatag tagttgtga ttacaggaa aaaatataa aataaccac atccaatca cagtcaatt aatccagcg tacgacgaa catcactct tgtaaacac aaacatgct gcgaataac cggcggaac gattctcaa gacgaaaaa aatacctaa actacaaat tcacaaata ctccaccga aaatcagtt gccgaacca agcagatgt tgtggttat cgggggtgg cgtggccgg ttgtagtct cctctacaa cttatccaa aagaggcgt taaggccgt tttgctaga aacagccaa aatcacaag tggcaccac ttggcacac aagcgctct gtgttggag gctaagacc ttgtgatgt tgatatcaa attgttgga atctaccag gaaacttct cattgattt ggaacaaga aacgggact taatacagg atggattaa taatggggg gcttttcat tgctaaaac agaggaacg aatccaaga atacaaacg tcttcacac cttcggcca ctttttcaa cattgaaag ccatataat ctcacctca agaagcttc aaaactctc accaatgct cgaccctaa caagttcaa aggagccct ttattcacc tggcgatgg ctttactga cccttccat gtactgtgc agcccttat aaaaggcgc cactacaaa aggggggca agtaatcga aaattgttc agttacaaa tatcctaac tgaccaatc aacaaacgt gcgaaaaat cacaggcgt ggaaacatc gaaaggtac catcaaaac tagctgtgt tgtgaacgc tggtggagt ttggtcgaa aaatattgc cgatctagt aggtctaga cctaccagt ggtaccaat gaaacattc atacatcat caccgaaag tatacctga agtgaaaaa cacaccaaa tattcgtga ccacgatgg gtccattta tctccgaat tcagggtga tagtgtcta tctaggtgg ctatgaaaa caacccgga aataatcag agacatgcc gtccgattt ccagtttag tttgttcga actggataa aagtctgtt tgaggtgca tttaaaaag tgccatgga tatagcccc agtttttgg caatatagg gatcaaatc ggatgtttg tggggctga ggcgttcac acctgacca taaacccat catgggtga ggatcccag gcttgcagg ttatttcta ctcgtgtgg gtacaattc gttgggtat aaacttgag tggtggctg tgccgaaca gttgggcat atggataac gaaaggtcg gcccgaaga accattatt tgcgtatga tataaggag atttacgcc caaccaacg atcggatcg ggcttgggt taccgaaac tagtcaaga atgctatgc gaaaaatta tagtattgt ttttccaca tgatcaacc tttagctgg acgaaatca taaaattgg accgtttca tgaggcttt agtcgccgg cggtgctgt aatggagca ggcccaagg ctgggaacg tccaggtta cttcgtcaa agacaggac agcccccgt gcggggcta cgactggta cggctatta cgaccacgt cgacaatcc cgacaaaag atacgagca agaactgga gggcgatta cactttcgg cttctccaa acatcacga tttgattgg ggaggaagc caatgccgc tcgaaacaa cgttgcact tttcgacct ctcctactt caccaaaat gtacttaac aggtcccga tgctgaaaa agctgccga ttggatttt cacagctga tacagataa agaaccagg acgtgttgt ttatacttg ttctttgaa tagtagagg tggggtcga ggctgatgt gactgttac agcattgga ggaaggtgt cggaactct agttgggcc cattttgaa ggggaaagg ttactatat tgtggctgg tggcgcatc aggatacca aacagtctc acacttcaa gaaagaact agccaagaa gaatttcaa agctgttat tagtgatat tacggataa gttaggagt tctttcaat tcagggacc caaaagtcg ggaactttt acaatcaat tactgaaac accaatcac tgatgagaa attcccacc tgggatgtc gcaccttat taaagtcaa tggtcacgt gtgccgtgc catgagggt tagttttat tggagaatt gggatatga attgcacgt tcctgtggc atcgtgtgt tgccgtttt taacaaagt tgtggatgc tggacgagg gtttgactt gagacatgc cggtttccg cgcgttgta ttctctgag tcttgagaa aggttacca cttgtggaa tcacgacct gcgaattga tgataatcc cgttgaagc gaatttgca agggatttg tcggaagga tggacaata cctaggtaa acaacatgt agagaaatt gaaggaaga aggggtgaa gaaacgtag agttttctt cacattgga ggatcaagt cgctcttta tggcttgga gaccatttg gagggacga taccattgt ggggtattt gagaagagg ggattatgg atttaacct cgattgtag tattggcac tggatatat tgaacatcc caagggaaa aattgtgac ggatgaatt cctcaaaag tggaaatta tcagattga agttttgga taaaaggta tccagcgac tgtgttttt gaagagtcc cttcgatcc gaaaaatca gaggctgtt aggacagta cgaccaaca gtttgagga acagtcgca ttttgagga ttaatattg ttgttgttt tataatttt taattgtaa taaaaaata aaaaccata
