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Gene information
General information
TC008511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11939903..11954464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008511
    iBeetle screen iB_01360

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 37.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    ovariole mostly decreased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008511 RA
      MEQKPAPKG RGALLEMLK KHKEARAGG AGEPVEEQA 
      PPKTRGRAM LLQKIQEAK ERKAGGDSG QLSTPGPST 
      VPSETRRGV SGVTKALGE VAITASETC SYRGESGTP 
      IKATANYIL LNVEKDRGV FEYEVRFQP DIDAKSNRI 
      KLVNQALGE LSTTKVYDG DVCLYLPCL AFSPRQEFE 
      SVIPNTETP VTTTLIYKR KRKLSECLH LYNVLFKRI 
      MHILLYQRM GRNYFSPDH KYLVPQHKL EVLPGFCVH 
      VDEMEGGLM VCLDTQHRV IRSQTVYEL FHEIRATNP 
      RNFREEVTK NVIGACVLT KYNNRTYII DDIAWNMNP 
      KDTFEDRSK GPSCFIDYY REHYNIRIE DVDQPLLIT 
      RQVKQSPDG KIERMICLI PELCYLTGL TDAMRNDFK 
      VMKDVAAFT RITPNQRML ALRTYLDRV RQSEKAKQV 
      LSGWGLSLA DDTVDVKAR VLPQEAIYF GGPDAEAHK 
      YTGGTDWNK AISDNKLTG PVNITNWQL YYTRRDQKY 
      AANFAQTIV RLGKGMGCV IQDPRHIVL DDDRTETYM 
      TAIRDNVAN TQVAVFICP TLRADRYSI IKKMCCVNI 
      PVASQVILS KTLSNPQKV RTIIHKIAM QITCKLGGT 
      LWSVKIPVS GWMVCGIDV YHGANNQSV CGFVASING 
      SMTKYFSKA MFQDGEIGD YFKMPFRQM LQAAKDREG 
      AFPSKVIVF RDGVGDGQL EHCRKYEIT QLQEVIKEL 
      NIETTITFV VVQKRINTR IFRTVNETN FENPPSGTV 
      VDNMVTRRQ FYDFFLVPQ SVRQGTVNP THYVVLVDE 
      GNIKPDHLQ RLAYKLCHL YYNWSGTIR VPAPCLYAH 
      KLAAIVGQY IKKTPSTQL DDKLFYL
    CDS sequence
    • >TC008511 RA
      atggaacag aaacctgct cccaagggc agaggggcc 
      ctcctcgag atgctcaag aagcataaa gaggctcgg 
      gctgggggc gctggagag cctgtagag gaacaggca 
      ccccccaaa actcgcgga agggcgatg cttctacag 
      aagattcag gaggctaag gagaggaaa gctgggggc 
      gacagtggg cagctttca acgccgggg ccttcgacg 
      gtgccttcg gagacaagg aggggcgtt tcaggggtc 
      acaaaggcg ttgggcgaa gttgcgatc actgcctcg 
      gagacgtgc tcttacaga ggtgagagt ggaactcca 
      ataaaagcc actgccaat tatattctc ctaaacgtc 
      gaaaaagac cgtggcgtt tttgaatac gaggtccga 
      tttcaaccc gatatcgac gccaaatca aacagaatt 
      aaactagta aatcaagct cttggagaa ttaagcacg 
      acaaaagtg tatgacgga gacgtatgt ctctactta 
      ccatgtctt gcattttct cctagacaa gaattcgaa 
      agtgttata cccaacaca gaaactccc gtcacaaca 
      acactaatt tataaaaga aagagaaaa ttgagcgaa 
      tgtttacat ttgtacaat gtgctattt aagagaatt 
      atgcacatt ttactctac caacgaatg ggacgaaat 
      tacttcagc ccagatcat aaatatctt gtgcctcaa 
      cataagctc gaagtattg cctggattt tgtgtgcat 
      gttgatgag atggaaggt ggtttgatg gtgtgtttg 
      gatacacaa catcgtgta attcgatca caaactgtt 
      tatgagcta tttcacgaa attagagca acgaatcct 
      cgaaatttt cgtgaggaa gttacaaag aatgtaata 
      ggagcttgt gttcttaca aaatataat aataggacg 
      tatattatc gatgatatt gcgtggaat atgaatccg 
      aaagatacg tttgaagat cgaagtaag gggccatct 
      tgttttatc gattactac agggaacat tataacatt 
      aggattgaa gatgtggat cagccattg cttattact 
      cgtcaagtt aaacaatcg cctgatggg aaaattgag 
      cgtatgatt tgtttgatt cctgaattg tgttatctt 
      acgggactt accgacgcg atgaggaac gattttaag 
      gtgatgaaa gatgtagca gctttcact cgtatcact 
      ccaaatcag cgaatgttg gcattgcgc acatatctg 
      gatcgagtt agacaatca gaaaaggct aagcaagta 
      ttgagcggt tggggtttg agtcttgct gacgatact 
      gttgacgta aaagcgcga gttttacct caagaagcg 
      atttatttt ggaggacct gatgctgaa gcacataaa 
      tataccggc ggaaccgat tggaataaa gctatatcc 
      gataacaaa cttactgga cctgttaat ataacaaat 
      tggcaattg tattacaca cgaagagat cagaaatat 
      gcggcaaat tttgcacaa acaattgta cgattggga 
      aaaggtatg ggatgtgtt atccaggat cctcgtcat 
      attgtctta gatgatgat cgaacggag acttatatg 
      actgcaatc agagacaat gtggcaaac acacaggtg 
      gcagtattt atctgtcca accctccgt gcagatcgc 
      tattccatc attaaaaaa atgtgttgc gtaaacatt 
      ccagttgcc agtcaggtc attttaagc aaaaccctt 
      tcaaatcca caaaaagtg cgcacaata attcacaaa 
      atcgcaatg caaatcact tgcaaactt ggcggcact 
      ttatggagt gtaaaaatt ccagtcagt ggttggatg 
      gtttgtgga attgatgtt tatcacggt gcaaacaat 
      caatccgtt tgcggtttc gttgcttca attaacggt 
      tcgatgacc aaatacttc agcaaagca atgtttcaa 
      gacggagaa attggtgat tattttaaa atgccgttt 
      cgtcaaatg ctccaagcg gctaaagat cgagaaggt 
      gcttttccg agtaaagta atcgttttt cgtgatgga 
      gtaggcgat ggtcaattg gaacattgc agaaaatac 
      gaaataaca cagctacag gaagttatc aaagaactg 
      aacatcgaa acgactata acttttgta gttgtgcaa 
      aaacggatc aatactcga atttttcga acggttaat 
      gagacgaat tttgaaaat cctccatct gggacagtg 
      gttgataac atggtcaca cgaaggcaa ttctacgac 
      tttttcctt gtgccgcag tcggttcgg caaggtact 
      gttaaccca actcattat gttgtgcta gtagacgaa 
      ggtaatatc aaacctgat catttacaa agacttgca 
      tataaactg tgtcatttg tattataac tggtcggga 
      actatcaga gtacctgcg ccttgtctt tatgcgcat 
      aagttagct gcgattgtg gggcaatat ataaagaaa 
      acacccagt acacaactg gatgataaa cttttttat 
      ttgtaa
    mRNA sequence
    • >TC008511 RA
      gttcttgta acggttttc tttaagtca ttgatacgc 
      tgttatcag tgtttttac tgcacaatt acaagctac 
      aaggcagta gtgaaatgt tactggtgt tgaaagctg 
      tgcttattt atggtaata ggaaacacg aacaagttt 
      acagaggtt tagtaaatc agtgagaca tttaagagt 
      aaccacgtt tgccttatt tgtcaacat attcaactt 
      cggcagaat ttaatcatc tgcttataa gtttaaaaa 
      tggaacaga aacctgctc ccaagggca gaggggccc 
      tcctcgaga tgctcaaga agcataaag aggctcggg 
      ctgggggcg ctggagagc ctgtagagg aacaggcac 
      cccccaaaa ctcgcggaa gggcgatgc ttctacaga 
      agattcagg aggctaagg agaggaaag ctgggggcg 
      acagtgggc agctttcaa cgccggggc cttcgacgg 
      tgccttcgg agacaagga ggggcgttt caggggtca 
      caaaggcgt tgggcgaag ttgcgatca ctgcctcgg 
      agacgtgct cttacagag gtgagagtg gaactccaa 
      taaaagcca ctgccaatt atattctcc taaacgtcg 
      aaaaagacc gtggcgttt ttgaatacg aggtccgat 
      ttcaacccg atatcgacg ccaaatcaa acagaatta 
      aactagtaa atcaagctc ttggagaat taagcacga 
      caaaagtgt atgacggag acgtatgtc tctacttac 
      catgtcttg cattttctc ctagacaag aattcgaaa 
      gtgttatac ccaacacag aaactcccg tcacaacaa 
      cactaattt ataaaagaa agagaaaat tgagcgaat 
      gtttacatt tgtacaatg tgctattta agagaatta 
      tgcacattt tactctacc aacgaatgg gacgaaatt 
      acttcagcc cagatcata aatatcttg tgcctcaac 
      ataagctcg aagtattgc ctggatttt gtgtgcatg 
      ttgatgaga tggaaggtg gtttgatgg tgtgtttgg 
      atacacaac atcgtgtaa ttcgatcac aaactgttt 
      atgagctat ttcacgaaa ttagagcaa cgaatcctc 
      gaaattttc gtgaggaag ttacaaaga atgtaatag 
      gagcttgtg ttcttacaa aatataata ataggacgt 
      atattatcg atgatattg cgtggaata tgaatccga 
      aagatacgt ttgaagatc gaagtaagg ggccatctt 
      gttttatcg attactaca gggaacatt ataacatta 
      ggattgaag atgtggatc agccattgc ttattactc 
      gtcaagtta aacaatcgc ctgatggga aaattgagc 
      gtatgattt gtttgattc ctgaattgt gttatctta 
      cgggactta ccgacgcga tgaggaacg attttaagg 
      tgatgaaag atgtagcag ctttcactc gtatcactc 
      caaatcagc gaatgttgg cattgcgca catatctgg 
      atcgagtta gacaatcag aaaaggcta agcaagtat 
      tgagcggtt ggggtttga gtcttgctg acgatactg 
      ttgacgtaa aagcgcgag ttttacctc aagaagcga 
      tttattttg gaggacctg atgctgaag cacataaat 
      ataccggcg gaaccgatt ggaataaag ctatatccg 
      ataacaaac ttactggac ctgttaata taacaaatt 
      ggcaattgt attacacac gaagagatc agaaatatg 
      cggcaaatt ttgcacaaa caattgtac gattgggaa 
      aaggtatgg gatgtgtta tccaggatc ctcgtcata 
      ttgtcttag atgatgatc gaacggaga cttatatga 
      ctgcaatca gagacaatg tggcaaaca cacaggtgg 
      cagtattta tctgtccaa ccctccgtg cagatcgct 
      attccatca ttaaaaaaa tgtgttgcg taaacattc 
      cagttgcca gtcaggtca ttttaagca aaacccttt 
      caaatccac aaaaagtgc gcacaataa ttcacaaaa 
      tcgcaatgc aaatcactt gcaaacttg gcggcactt 
      tatggagtg taaaaattc cagtcagtg gttggatgg 
      tttgtggaa ttgatgttt atcacggtg caaacaatc 
      aatccgttt gcggtttcg ttgcttcaa ttaacggtt 
      cgatgacca aatacttca gcaaagcaa tgtttcaag 
      acggagaaa ttggtgatt attttaaaa tgccgtttc 
      gtcaaatgc tccaagcgg ctaaagatc gagaaggtg 
      cttttccga gtaaagtaa tcgtttttc gtgatggag 
      taggcgatg gtcaattgg aacattgca gaaaatacg 
      aaataacac agctacagg aagttatca aagaactga 
      acatcgaaa cgactataa cttttgtag ttgtgcaaa 
      aacggatca atactcgaa tttttcgaa cggttaatg 
      agacgaatt ttgaaaatc ctccatctg ggacagtgg 
      ttgataaca tggtcacac gaaggcaat tctacgact 
      ttttccttg tgccgcagt cggttcggc aaggtactg 
      ttaacccaa ctcattatg ttgtgctag tagacgaag 
      gtaatatca aacctgatc atttacaaa gacttgcat 
      ataaactgt gtcatttgt attataact ggtcgggaa 
      ctatcagag tacctgcgc cttgtcttt atgcgcata 
      agttagctg cgattgtgg ggcaatata taaagaaaa 
      cacccagta cacaactgg atgataaac ttttttatt 
      tgtaaaaaa tacattttt attgattgt gttaggctt 
      atgccagaa ataattctc gtttttttt tttttcgaa 
      tatttttgt taccactca atttttatg attatattt 
      ttatacgga cataataaa gtgccattt attta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY