Status
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Gene information
General information
TC008511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11939903..11954464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008511
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01360

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 37.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Fertility
    ovariole mostly decreased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008511 RA
      MEQKPAPKG RGALLEMLK KHKEARAGG AGEPVEEQA 
PPKTRGRAM LLQKIQEAK ERKAGGDSG QLSTPGPST 
VPSETRRGV SGVTKALGE VAITASETC SYRGESGTP 
IKATANYIL LNVEKDRGV FEYEVRFQP DIDAKSNRI 
KLVNQALGE LSTTKVYDG DVCLYLPCL AFSPRQEFE 
SVIPNTETP VTTTLIYKR KRKLSECLH LYNVLFKRI 
MHILLYQRM GRNYFSPDH KYLVPQHKL EVLPGFCVH 
VDEMEGGLM VCLDTQHRV IRSQTVYEL FHEIRATNP 
RNFREEVTK NVIGACVLT KYNNRTYII DDIAWNMNP 
KDTFEDRSK GPSCFIDYY REHYNIRIE DVDQPLLIT 
RQVKQSPDG KIERMICLI PELCYLTGL TDAMRNDFK 
VMKDVAAFT RITPNQRML ALRTYLDRV RQSEKAKQV 
LSGWGLSLA DDTVDVKAR VLPQEAIYF GGPDAEAHK 
YTGGTDWNK AISDNKLTG PVNITNWQL YYTRRDQKY 
AANFAQTIV RLGKGMGCV IQDPRHIVL DDDRTETYM 
TAIRDNVAN TQVAVFICP TLRADRYSI IKKMCCVNI 
PVASQVILS KTLSNPQKV RTIIHKIAM QITCKLGGT 
LWSVKIPVS GWMVCGIDV YHGANNQSV CGFVASING 
SMTKYFSKA MFQDGEIGD YFKMPFRQM LQAAKDREG 
AFPSKVIVF RDGVGDGQL EHCRKYEIT QLQEVIKEL 
NIETTITFV VVQKRINTR IFRTVNETN FENPPSGTV 
VDNMVTRRQ FYDFFLVPQ SVRQGTVNP THYVVLVDE 
GNIKPDHLQ RLAYKLCHL YYNWSGTIR VPAPCLYAH 
KLAAIVGQY IKKTPSTQL DDKLFYL
    CDS sequence
    • >TC008511 RA
      atggaacag aaacctgct cccaagggc agaggggcc 
ctcctcgag atgctcaag aagcataaa gaggctcgg 
gctgggggc gctggagag cctgtagag gaacaggca 
ccccccaaa actcgcgga agggcgatg cttctacag 
aagattcag gaggctaag gagaggaaa gctgggggc 
gacagtggg cagctttca acgccgggg ccttcgacg 
gtgccttcg gagacaagg aggggcgtt tcaggggtc 
acaaaggcg ttgggcgaa gttgcgatc actgcctcg 
gagacgtgc tcttacaga ggtgagagt ggaactcca 
ataaaagcc actgccaat tatattctc ctaaacgtc 
gaaaaagac cgtggcgtt tttgaatac gaggtccga 
tttcaaccc gatatcgac gccaaatca aacagaatt 
aaactagta aatcaagct cttggagaa ttaagcacg 
acaaaagtg tatgacgga gacgtatgt ctctactta 
ccatgtctt gcattttct cctagacaa gaattcgaa 
agtgttata cccaacaca gaaactccc gtcacaaca 
acactaatt tataaaaga aagagaaaa ttgagcgaa 
tgtttacat ttgtacaat gtgctattt aagagaatt 
atgcacatt ttactctac caacgaatg ggacgaaat 
tacttcagc ccagatcat aaatatctt gtgcctcaa 
cataagctc gaagtattg cctggattt tgtgtgcat 
gttgatgag atggaaggt ggtttgatg gtgtgtttg 
gatacacaa catcgtgta attcgatca caaactgtt 
tatgagcta tttcacgaa attagagca acgaatcct 
cgaaatttt cgtgaggaa gttacaaag aatgtaata 
ggagcttgt gttcttaca aaatataat aataggacg 
tatattatc gatgatatt gcgtggaat atgaatccg 
aaagatacg tttgaagat cgaagtaag gggccatct 
tgttttatc gattactac agggaacat tataacatt 
aggattgaa gatgtggat cagccattg cttattact 
cgtcaagtt aaacaatcg cctgatggg aaaattgag 
cgtatgatt tgtttgatt cctgaattg tgttatctt 
acgggactt accgacgcg atgaggaac gattttaag 
gtgatgaaa gatgtagca gctttcact cgtatcact 
ccaaatcag cgaatgttg gcattgcgc acatatctg 
gatcgagtt agacaatca gaaaaggct aagcaagta 
ttgagcggt tggggtttg agtcttgct gacgatact 
gttgacgta aaagcgcga gttttacct caagaagcg 
atttatttt ggaggacct gatgctgaa gcacataaa 
tataccggc ggaaccgat tggaataaa gctatatcc 
gataacaaa cttactgga cctgttaat ataacaaat 
tggcaattg tattacaca cgaagagat cagaaatat 
gcggcaaat tttgcacaa acaattgta cgattggga 
aaaggtatg ggatgtgtt atccaggat cctcgtcat 
attgtctta gatgatgat cgaacggag acttatatg 
actgcaatc agagacaat gtggcaaac acacaggtg 
gcagtattt atctgtcca accctccgt gcagatcgc 
tattccatc attaaaaaa atgtgttgc gtaaacatt 
ccagttgcc agtcaggtc attttaagc aaaaccctt 
tcaaatcca caaaaagtg cgcacaata attcacaaa 
atcgcaatg caaatcact tgcaaactt ggcggcact 
ttatggagt gtaaaaatt ccagtcagt ggttggatg 
gtttgtgga attgatgtt tatcacggt gcaaacaat 
caatccgtt tgcggtttc gttgcttca attaacggt 
tcgatgacc aaatacttc agcaaagca atgtttcaa 
gacggagaa attggtgat tattttaaa atgccgttt 
cgtcaaatg ctccaagcg gctaaagat cgagaaggt 
gcttttccg agtaaagta atcgttttt cgtgatgga 
gtaggcgat ggtcaattg gaacattgc agaaaatac 
gaaataaca cagctacag gaagttatc aaagaactg 
aacatcgaa acgactata acttttgta gttgtgcaa 
aaacggatc aatactcga atttttcga acggttaat 
gagacgaat tttgaaaat cctccatct gggacagtg 
gttgataac atggtcaca cgaaggcaa ttctacgac 
tttttcctt gtgccgcag tcggttcgg caaggtact 
gttaaccca actcattat gttgtgcta gtagacgaa 
ggtaatatc aaacctgat catttacaa agacttgca 
tataaactg tgtcatttg tattataac tggtcggga 
actatcaga gtacctgcg ccttgtctt tatgcgcat 
aagttagct gcgattgtg gggcaatat ataaagaaa 
acacccagt acacaactg gatgataaa cttttttat 
ttgtaa
    mRNA sequence
    • >TC008511 RA
      gttcttgta acggttttc tttaagtca ttgatacgc 
tgttatcag tgtttttac tgcacaatt acaagctac 
aaggcagta gtgaaatgt tactggtgt tgaaagctg 
tgcttattt atggtaata ggaaacacg aacaagttt 
acagaggtt tagtaaatc agtgagaca tttaagagt 
aaccacgtt tgccttatt tgtcaacat attcaactt 
cggcagaat ttaatcatc tgcttataa gtttaaaaa 
tggaacaga aacctgctc ccaagggca gaggggccc 
tcctcgaga tgctcaaga agcataaag aggctcggg 
ctgggggcg ctggagagc ctgtagagg aacaggcac 
cccccaaaa ctcgcggaa gggcgatgc ttctacaga 
agattcagg aggctaagg agaggaaag ctgggggcg 
acagtgggc agctttcaa cgccggggc cttcgacgg 
tgccttcgg agacaagga ggggcgttt caggggtca 
caaaggcgt tgggcgaag ttgcgatca ctgcctcgg 
agacgtgct cttacagag gtgagagtg gaactccaa 
taaaagcca ctgccaatt atattctcc taaacgtcg 
aaaaagacc gtggcgttt ttgaatacg aggtccgat 
ttcaacccg atatcgacg ccaaatcaa acagaatta 
aactagtaa atcaagctc ttggagaat taagcacga 
caaaagtgt atgacggag acgtatgtc tctacttac 
catgtcttg cattttctc ctagacaag aattcgaaa 
gtgttatac ccaacacag aaactcccg tcacaacaa 
cactaattt ataaaagaa agagaaaat tgagcgaat 
gtttacatt tgtacaatg tgctattta agagaatta 
tgcacattt tactctacc aacgaatgg gacgaaatt 
acttcagcc cagatcata aatatcttg tgcctcaac 
ataagctcg aagtattgc ctggatttt gtgtgcatg 
ttgatgaga tggaaggtg gtttgatgg tgtgtttgg 
atacacaac atcgtgtaa ttcgatcac aaactgttt 
atgagctat ttcacgaaa ttagagcaa cgaatcctc 
gaaattttc gtgaggaag ttacaaaga atgtaatag 
gagcttgtg ttcttacaa aatataata ataggacgt 
atattatcg atgatattg cgtggaata tgaatccga 
aagatacgt ttgaagatc gaagtaagg ggccatctt 
gttttatcg attactaca gggaacatt ataacatta 
ggattgaag atgtggatc agccattgc ttattactc 
gtcaagtta aacaatcgc ctgatggga aaattgagc 
gtatgattt gtttgattc ctgaattgt gttatctta 
cgggactta ccgacgcga tgaggaacg attttaagg 
tgatgaaag atgtagcag ctttcactc gtatcactc 
caaatcagc gaatgttgg cattgcgca catatctgg 
atcgagtta gacaatcag aaaaggcta agcaagtat 
tgagcggtt ggggtttga gtcttgctg acgatactg 
ttgacgtaa aagcgcgag ttttacctc aagaagcga 
tttattttg gaggacctg atgctgaag cacataaat 
ataccggcg gaaccgatt ggaataaag ctatatccg 
ataacaaac ttactggac ctgttaata taacaaatt 
ggcaattgt attacacac gaagagatc agaaatatg 
cggcaaatt ttgcacaaa caattgtac gattgggaa 
aaggtatgg gatgtgtta tccaggatc ctcgtcata 
ttgtcttag atgatgatc gaacggaga cttatatga 
ctgcaatca gagacaatg tggcaaaca cacaggtgg 
cagtattta tctgtccaa ccctccgtg cagatcgct 
attccatca ttaaaaaaa tgtgttgcg taaacattc 
cagttgcca gtcaggtca ttttaagca aaacccttt 
caaatccac aaaaagtgc gcacaataa ttcacaaaa 
tcgcaatgc aaatcactt gcaaacttg gcggcactt 
tatggagtg taaaaattc cagtcagtg gttggatgg 
tttgtggaa ttgatgttt atcacggtg caaacaatc 
aatccgttt gcggtttcg ttgcttcaa ttaacggtt 
cgatgacca aatacttca gcaaagcaa tgtttcaag 
acggagaaa ttggtgatt attttaaaa tgccgtttc 
gtcaaatgc tccaagcgg ctaaagatc gagaaggtg 
cttttccga gtaaagtaa tcgtttttc gtgatggag 
taggcgatg gtcaattgg aacattgca gaaaatacg 
aaataacac agctacagg aagttatca aagaactga 
acatcgaaa cgactataa cttttgtag ttgtgcaaa 
aacggatca atactcgaa tttttcgaa cggttaatg 
agacgaatt ttgaaaatc ctccatctg ggacagtgg 
ttgataaca tggtcacac gaaggcaat tctacgact 
ttttccttg tgccgcagt cggttcggc aaggtactg 
ttaacccaa ctcattatg ttgtgctag tagacgaag 
gtaatatca aacctgatc atttacaaa gacttgcat 
ataaactgt gtcatttgt attataact ggtcgggaa 
ctatcagag tacctgcgc cttgtcttt atgcgcata 
agttagctg cgattgtgg ggcaatata taaagaaaa 
cacccagta cacaactgg atgataaac ttttttatt 
tgtaaaaaa tacattttt attgattgt gttaggctt 
atgccagaa ataattctc gtttttttt tttttcgaa 
tatttttgt taccactca atttttatg attatattt 
ttatacgga cataataaa gtgccattt attta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY