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Gene information
General information
TC008507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid34: 165341..186497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008507
    iBeetle screen iB_01359
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi) mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 2
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 6

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008507 RA
      MESEKSTES ELTSTMGNL LSSNTDEKS ESCDSTPTS 
      STDAFTTTS TSHIAADDP ISSTSSDAL DSGKHPSSA 
      NPVINLVRE MSGGGNPSP IASDKDLLD MNITDTSTE 
      KITENVDDI VQDLENLLG EPTTSFDFT IRKDLRGAN 
      KDIAMELDE FSAELQMLQ EKAVEPTTV ESKEPSDSV 
      TQSDIDNKI NDSVEVSTE NDLSELTPH ISSTDPKID 
      ANESTSILN EEKLGSLGI EQNVPQLDN EPKSVEENK 
      NNDNISSKC DKEPEDEKQ ECLDIEQMV PQSGSEEVE 
      KQVIEEEKQ QSLDIEQRV SQIDSDPQS VEIIENKES 
      AEEVEEKEL KEEKQESLE VEQMVPQLY NEPQCVEED 
      VSSKSREQA EKKRMIEGG DSIVNISIA SLAKIEENK 
      ETESDQVDQ MTVVSEKLD EKTMEIGRE VFKTAEDLK 
      EKREGIKTI EEEKNMEIR CEDSKTVED REVYNEGIK 
      AIEEDKSME IGSEDLKTV ADTEVNCPE ENVEGIKTI 
      EGLKIMEIG NRDLRTVDD SEVNSSEEK SEDIKTNDE 
      EKNMEIGRV ENMFKGIED SEVNTSQEK SMEIISEEQ 
      IGSKDLETG EASEAISPD EKSVETIGK RIITVGEEK 
      SMEIIGEGI KTKEIGSKD LKTIEDSEV NSPKEKSLE 
      TIGKAIESV KEEKNMEIR SEDFKTVAS EVNSPKEKS 
      MNIVDEVIN TVEDSEDNN PEVEFVAVA GGESNERTD 
      EKNVEDETE ETDLAPIDF IPEPKVMQA EEEPHLPTS 
      SVSDLNVLD KSNVLPTAE EIFAEQDEN LETLLAVAS 
      LGNVDYNMP PEEFVPEAS PELTPFASK ELDERIESL 
      FIEKGLSKE QQGDKLKEP LVINVDDTE KHRVYSPKI 
      TIKAIKSPE ETEEGSKGS LKMTITKQS DNTHSILKI 
      YNPEEESQN QDEPTIPKL IIKPIVQPN EQPQSPKTS 
      KSSKQVFSP SQRSSSPRI TIKPIPEPL APLKITIKP 
      VERQKHSPK LTIKPIVEP VPKVTIKPV LKPQEKDEQ 
      IEKSSPKIT IKPIVKPQE LETTDEDDS VKERIVLKI 
      NKGTIPGSS KEEKLTKIK LKFSKEGGH PHIVQDDES 
      PSKRQRIDS EMLPGVTIT PITSQHSKL KEMLSRGVD 
      KVVTIDDDD DDVAIVEET RKPSPIVIS EESNQDSNN 
      AMRDPMADI PIFEITPGS VKTMNPQPV VEEIVTVPT 
      PRKRGRPRK VPLQVREDF KDPKEDEPP KVVVESTRP 
      KRSCRGQSV RTTLGIKPR KPRGPGRGR GSKRGMGTR 
      STPTPPEMS PKGSKEPKL ASAKEDLLL LSDKTEVKH 
      IEIDEGFHK SPTQSLLAP PQDTSSSTS DRADIVMYE 
      EETRMSAET SSRAQTPAK QVVTSIPSV DLIEESQSS 
      VQSTATTES VKVRKGPRL EVHQEPEGA VISADQLAE 
      YYWSGGGPY MLQEQVAQF LGIKSFKRK YPNIQRRVV 
      DMQERDFIR ESGLASEAM CDLGLTAVN TADILDIMY 
      SDFQDKYEE YCKHQRDRQ AKDLTNKQK ALSLAAMQE 
      KTKMDITQQ AVQSAASWN STFNKTRRE QRKACMDLQ 
      TLTVHFPKG KMKQINKPK IGHYPVALV PGQFTDYYR 
      EFTPTEINN LPLNTMCYN TLIRGRHHE MECDSQTDG 
      SGSESDTST SDSDSSYSG SSDAEDCTL CKPTPKRLA 
      AAL
    CDS sequence
    • >TC008507 RA
      atggaaagt gaaaaatcc accgaaagc gagttaacc 
      tcaacaatg ggcaacctg ctgtcatcg aataccgac 
      gaaaaaagt gagtcttgt gattcaacc ccaacgtct 
      tccaccgat gcatttaca accacttca acgtcacac 
      attgctgct gacgatcca atcagtagc acatcctcg 
      gatgctttg gattcgggg aaacacccg agtagtgcg 
      aacccagtt attaattta gtgagggag atgtcaggg 
      ggtggtaac ccatcgccg attgcgtcc gataaggac 
      ctgctcgat atgaatatt acggatact tctacggag 
      aaaattaca gaaaatgtt gatgacatt gttcaggat 
      ttggagaat ttgttaggg gagccgacg acttcgttt 
      gattttacg attcgaaag gatctgaga ggggctaat 
      aaagacatt gcgatggag cttgatgag ttttcagct 
      gaacttcaa atgttgcaa gaaaaagca gttgagccc 
      actacggtg gaatcaaaa gaaccatct gattcggtt 
      actcaatcc gatattgat aataagatc aacgattct 
      gtcgaagtt agtacagaa aatgattta tctgaattg 
      acaccgcac atatcatca acggatcca aaaattgat 
      gcaaatgaa agtacgtca attcttaac gaagaaaag 
      cttggtagc ttaggtatt gaacaaaat gtaccacag 
      cttgacaat gaaccgaaa agcgtggaa gaaaataaa 
      aataatgat aatatttct tcaaaatgt gataaagaa 
      ccagaagac gaaaagcaa gaatgttta gatatcgag 
      caaatggtt ccacaaagt ggaagtgaa gaagttgaa 
      aaacaagtg atcgaagaa gaaaaacaa caaagttta 
      gatattgag caaagggtt tcacagatt gacagtgat 
      ccacaaagt gtggaaata attgaaaat aaagaatct 
      gctgaagaa gttgaagaa aaagagttg aaagaagaa 
      aaacaagaa agtttggaa gttgagcaa atggttcca 
      cagctttac aacgaacca caatgtgtg gaagaagat 
      gtttcttcc aaatcccgt gaacaggct gaaaaaaaa 
      aggatgata gaaggtgga gatagtatc gtcaacatt 
      tctattgca tcactagca aaaattgaa gagaataaa 
      gagactgaa tcagatcaa gttgatcaa atgactgtt 
      gtatctgaa aagttggat gaaaaaact atggaaatt 
      ggacgcgag gtcttcaaa actgctgaa gatttaaaa 
      gaaaaaaga gaaggcatc aaaactatt gaagaagag 
      aaaaatatg gaaattaga tgcgaggac tctaaaact 
      gtcgaagat cgagaagtt tataacgaa ggtatcaaa 
      gctattgaa gaagataaa agtatggaa attggaagt 
      gaggatttg aaaactgtt gcagataca gaagttaat 
      tgtccagaa gaaaatgtt gaagggatc aaaactatt 
      gaaggactt aaaattatg gaaattggg aaccgtgat 
      ttgaggact gttgatgat tcagaagtt aatagttca 
      gaagaaaaa agtgaagac atcaaaact aatgatgaa 
      gaaaaaaat atggaaatt ggacgtgtt gaaaacatg 
      ttcaaaggt attgaagat tcagaagtt aatacttca 
      caagaaaaa agtatggaa attattagt gaagaacaa 
      attggaagc aaggacttg gaaactggt gaagcttca 
      gaagctatt agcccagac gaaaaaagt gtggaaact 
      attggtaaa cgtatcata actgttgga gaagaaaaa 
      agtatggaa attattggt gaaggcatt aaaactaaa 
      gaaattgga agcaaggac ttgaaaacg attgaagat 
      tcagaagtt aatagccca aaagaaaaa agtttggaa 
      actattggt aaagccatc gaaagtgtt aaagaagaa 
      aaaaatatg gaaattaga agcgaggac tttaaaact 
      gttgcttca gaagttaac agtccaaaa gaaaaaagt 
      atgaatatt gttgatgaa gtcatcaat actgttgaa 
      gattcagaa gataataat ccagaagtt gagtttgtt 
      gctgttgct ggaggtgaa tcgaatgaa cgtacagat 
      gaaaaaaat gttgaagac gaaacggaa gaaacagat 
      cttgcacca attgatttt atacctgaa ccaaaagta 
      atgcaagct gaagaggaa cctcatctt ccaacttca 
      tctgtttct gatctcaac gtattagac aaatcgaat 
      gttttacca acagctgaa gaaattttt gccgaacaa 
      gatgaaaat ttggagaca ttattggct gttgcaagt 
      ttaggaaat gttgattat aatatgcca cctgaagag 
      tttgttccg gaagcatca cctgaacta accccgttt 
      gcttccaaa gaactagat gaacgaatc gagtcttta 
      tttattgag aaaggttta tcaaaagag caacaaggc 
      gacaaactt aaggagcct ctagtaatt aatgtggac 
      gatacggaa aaacatcga gtctatagt ccgaaaatt 
      acaatcaag gctatcaaa tctcctgag gagacggaa 
      gaagggagt aaaggatca ctaaaaatg acaattacc 
      aaacagtct gataacaca cattcgatt cttaagata 
      tataatcca gaggaggaa tcacaaaat caggacgaa 
      ccaacaatt ccgaaactt ataataaaa cctattgtc 
      caaccgaac gaacaacct caaagtcct aaaacttct 
      aaatcttct aaacaagtg tttagtcct agtcagaga 
      tcgtcaagt ccaaggatt acaattaaa ccaattcct 
      gaacctttg gctccatta aaaattaca ataaaacca 
      gttgaaagg caaaaacac agtccgaaa ctaacaatt 
      aaaccaatc gttgaaccg gtgccaaaa gtgacaata 
      aaacccgtc ttaaaacca caagaaaag gacgaacaa 
      attgaaaaa tccagtcca aaaattaca attaagcca 
      attgtgaaa ccgcaagaa ttggaaaca actgatgaa 
      gatgattcg gttaaggaa cgaattgtt ttgaaaata 
      aacaagggt acaatacct ggtagtagc aaggaggaa 
      aaattaact aaaatcaag ttaaaattt tccaaagaa 
      gggggtcac ccgcatatc gtccaagac gatgaatct 
      ccctctaaa agacaaaga atcgacagt gaaatgttg 
      ccgggtgtt actataact ccgatcaca tcgcaacat 
      tcaaaattg aaagagatg ttgagtcgt ggtgtcgat 
      aaagttgtg acgattgac gatgacgat gatgatgtt 
      gcgattgtt gaagaaacg cggaaaccg tcaccgatt 
      gtgattagt gaggaatcg aatcaggat agtaacaat 
      gctatgcgg gatccaatg gctgatatt cccattttt 
      gagattacg ccaggaagt gtgaaaacg atgaatccg 
      caaccggtt gtggaggag attgtgacg gtgcccacg 
      ccgaggaaa aggggaagg ccgcgaaag gttccgttg 
      caagttcgg gaagacttt aaggatcca aaagaagat 
      gaaccacct aaagttgtg gtcgagtca actcgtccc 
      aaacgaagt tgtcgagga cagtcagtg agaacaaca 
      cttggtatt aaaccccga aaaccaagg ggtcctgga 
      aggggcagg ggcagtaaa cgaggaatg ggcacaaga 
      agtacccca acacctccc gaaatgtca ccgaaaggc 
      tcaaaggaa ccgaaattg gccagtgct aaagaagat 
      ttgttactg ttgagtgat aaaactgaa gtcaaacac 
      atcgaaatc gatgaggga tttcacaaa tcaccaact 
      cagtcactc ttagcacca ccacaagat acttcttcc 
      tccacttcg gatagggct gatattgtg atgtatgaa 
      gaagagacg cgcatgagt gcagaaacg agtagcaga 
      gctcaaacg ccagccaaa caggtcgtc acatcaatt 
      ccaagcgtc gacctcatc gaggaatct caaagctca 
      gtccaaagc acagccacc acagaaagt gtcaaagta 
      cgcaaaggt ccacgttta gaagttcac caagaacct 
      gaaggtgca gtcatttca gccgatcaa cttgctgaa 
      tactattgg agtggtgga ggtccttat atgcttcaa 
      gaacaagtc gctcaattt ttgggcatc aaatcattc 
      aaacgaaaa tatcccaac attcagcga agagttgtt 
      gatatgcaa gagagagat tttattcgc gaaagtgga 
      ctagctagc gaagctatg tgcgatctt ggtttgaca 
      gctgtcaat acggccgat attttggat attatgtat 
      tcagacttt caagataaa tacgaagaa tactgcaaa 
      catcagcgc gataggcag gctaaggat ttgaccaat 
      aagcaaaaa gctttgagt ttggcggca atgcaggag 
      aagaccaag atggatatc acgcagcag gcggtgcaa 
      tcggcggcg agttggaat tctactttt aataagacg 
      cgacgagaa cagagaaag gcttgtatg gatttgcaa 
      accttaact gttcacttt ccaaaaggc aaaatgaag 
      caaatcaac aaacccaaa atcgggcat tatccagta 
      gccttagtt ccaggacaa tttacagac tattacagg 
      gaatttaca ccaactgaa ataaataat ttaccgctg 
      aatacaatg tgctacaat actttgata cgaggacga 
      catcatgaa atggaatgt gatagtcaa actgacggt 
      tcaggttct gagtcggac acaagcact tcagactcc 
      gatagttct tacagtggt tcttcagat gcagaagat 
      tgcactctt tgcaaacca acaccgaaa agactagct 
      gctgcactc taa
    mRNA sequence
    • >TC008507 RA
      ccacttgtg atggcgttt gtcgtttaa aaccgtaaa 
      caaaaatgc cgcatttaa ttatacccg taattaatt 
      gtctgtgat aaacggtgt gttttcaac caaatagca 
      ccattttcc cgtgcaagt gacaatgga aagtgaaaa 
      atccaccga aagcgagtt aacctcaac aatgggcaa 
      cctgctgtc atcgaatac cgacgaaaa aagtgagtc 
      ttgtgattc aaccccaac gtcttccac cgatgcatt 
      tacaaccac ttcaacgtc acacattgc tgctgacga 
      tccaatcag tagcacatc ctcggatgc tttggattc 
      ggggaaaca cccgagtag tgcgaaccc agttattaa 
      tttagtgag ggagatgtc agggggtgg taacccatc 
      gccgattgc gtccgataa ggacctgct cgatatgaa 
      tattacgga tacttctac ggagaaaat tacagaaaa 
      tgttgatga cattgttca ggatttgga gaatttgtt 
      aggggagcc gacgacttc gtttgattt tacgattcg 
      aaaggatct gagaggggc taataaaga cattgcgat 
      ggagcttga tgagttttc agctgaact tcaaatgtt 
      gcaagaaaa agcagttga gcccactac ggtggaatc 
      aaaagaacc atctgattc ggttactca atccgatat 
      tgataataa gatcaacga ttctgtcga agttagtac 
      agaaaatga tttatctga attgacacc gcacatatc 
      atcaacgga tccaaaaat tgatgcaaa tgaaagtac 
      gtcaattct taacgaaga aaagcttgg tagcttagg 
      tattgaaca aaatgtacc acagcttga caatgaacc 
      gaaaagcgt ggaagaaaa taaaaataa tgataatat 
      ttcttcaaa atgtgataa agaaccaga agacgaaaa 
      gcaagaatg tttagatat cgagcaaat ggttccaca 
      aagtggaag tgaagaagt tgaaaaaca agtgatcga 
      agaagaaaa acaacaaag tttagatat tgagcaaag 
      ggtttcaca gattgacag tgatccaca aagtgtgga 
      aataattga aaataaaga atctgctga agaagttga 
      agaaaaaga gttgaaaga agaaaaaca agaaagttt 
      ggaagttga gcaaatggt tccacagct ttacaacga 
      accacaatg tgtggaaga agatgtttc ttccaaatc 
      ccgtgaaca ggctgaaaa aaaaaggat gatagaagg 
      tggagatag tatcgtcaa catttctat tgcatcact 
      agcaaaaat tgaagagaa taaagagac tgaatcaga 
      tcaagttga tcaaatgac tgttgtatc tgaaaagtt 
      ggatgaaaa aactatgga aattggacg cgaggtctt 
      caaaactgc tgaagattt aaaagaaaa aagagaagg 
      catcaaaac tattgaaga agagaaaaa tatggaaat 
      tagatgcga ggactctaa aactgtcga agatcgaga 
      agtttataa cgaaggtat caaagctat tgaagaaga 
      taaaagtat ggaaattgg aagtgagga tttgaaaac 
      tgttgcaga tacagaagt taattgtcc agaagaaaa 
      tgttgaagg gatcaaaac tattgaagg acttaaaat 
      tatggaaat tgggaaccg tgatttgag gactgttga 
      tgattcaga agttaatag ttcagaaga aaaaagtga 
      agacatcaa aactaatga tgaagaaaa aaatatgga 
      aattggacg tgttgaaaa catgttcaa aggtattga 
      agattcaga agttaatac ttcacaaga aaaaagtat 
      ggaaattat tagtgaaga acaaattgg aagcaagga 
      cttggaaac tggtgaagc ttcagaagc tattagccc 
      agacgaaaa aagtgtgga aactattgg taaacgtat 
      cataactgt tggagaaga aaaaagtat ggaaattat 
      tggtgaagg cattaaaac taaagaaat tggaagcaa 
      ggacttgaa aacgattga agattcaga agttaatag 
      cccaaaaga aaaaagttt ggaaactat tggtaaagc 
      catcgaaag tgttaaaga agaaaaaaa tatggaaat 
      tagaagcga ggactttaa aactgttgc ttcagaagt 
      taacagtcc aaaagaaaa aagtatgaa tattgttga 
      tgaagtcat caatactgt tgaagattc agaagataa 
      taatccaga agttgagtt tgttgctgt tgctggagg 
      tgaatcgaa tgaacgtac agatgaaaa aaatgttga 
      agacgaaac ggaagaaac agatcttgc accaattga 
      ttttatacc tgaaccaaa agtaatgca agctgaaga 
      ggaacctca tcttccaac ttcatctgt ttctgatct 
      caacgtatt agacaaatc gaatgtttt accaacagc 
      tgaagaaat ttttgccga acaagatga aaatttgga 
      gacattatt ggctgttgc aagtttagg aaatgttga 
      ttataatat gccacctga agagtttgt tccggaagc 
      atcacctga actaacccc gtttgcttc caaagaact 
      agatgaacg aatcgagtc tttatttat tgagaaagg 
      tttatcaaa agagcaaca aggcgacaa acttaagga 
      gcctctagt aattaatgt ggacgatac ggaaaaaca 
      tcgagtcta tagtccgaa aattacaat caaggctat 
      caaatctcc tgaggagac ggaagaagg gagtaaagg 
      atcactaaa aatgacaat taccaaaca gtctgataa 
      cacacattc gattcttaa gatatataa tccagagga 
      ggaatcaca aaatcagga cgaaccaac aattccgaa 
      acttataat aaaacctat tgtccaacc gaacgaaca 
      acctcaaag tcctaaaac ttctaaatc ttctaaaca 
      agtgtttag tcctagtca gagatcgtc aagtccaag 
      gattacaat taaaccaat tcctgaacc tttggctcc 
      attaaaaat tacaataaa accagttga aaggcaaaa 
      acacagtcc gaaactaac aattaaacc aatcgttga 
      accggtgcc aaaagtgac aataaaacc cgtcttaaa 
      accacaaga aaaggacga acaaattga aaaatccag 
      tccaaaaat tacaattaa gccaattgt gaaaccgca 
      agaattgga aacaactga tgaagatga ttcggttaa 
      ggaacgaat tgttttgaa aataaacaa gggtacaat 
      acctggtag tagcaagga ggaaaaatt aactaaaat 
      caagttaaa attttccaa agaaggggg tcacccgca 
      tatcgtcca agacgatga atctccctc taaaagaca 
      aagaatcga cagtgaaat gttgccggg tgttactat 
      aactccgat cacatcgca acattcaaa attgaaaga 
      gatgttgag tcgtggtgt cgataaagt tgtgacgat 
      tgacgatga cgatgatga tgttgcgat tgttgaaga 
      aacgcggaa accgtcacc gattgtgat tagtgagga 
      atcgaatca ggatagtaa caatgctat gcgggatcc 
      aatggctga tattcccat ttttgagat tacgccagg 
      aagtgtgaa aacgatgaa tccgcaacc ggttgtgga 
      ggagattgt gacggtgcc cacgccgag gaaaagggg 
      aaggccgcg aaaggttcc gttgcaagt tcgggaaga 
      ctttaagga tccaaaaga agatgaacc acctaaagt 
      tgtggtcga gtcaactcg tcccaaacg aagttgtcg 
      aggacagtc agtgagaac aacacttgg tattaaacc 
      ccgaaaacc aaggggtcc tggaagggg caggggcag 
      taaacgagg aatgggcac aagaagtac cccaacacc 
      tcccgaaat gtcaccgaa aggctcaaa ggaaccgaa 
      attggccag tgctaaaga agatttgtt actgttgag 
      tgataaaac tgaagtcaa acacatcga aatcgatga 
      gggatttca caaatcacc aactcagtc actcttagc 
      accaccaca agatacttc ttcctccac ttcggatag 
      ggctgatat tgtgatgta tgaagaaga gacgcgcat 
      gagtgcaga aacgagtag cagagctca aacgccagc 
      caaacaggt cgtcacatc aattccaag cgtcgacct 
      catcgagga atctcaaag ctcagtcca aagcacagc 
      caccacaga aagtgtcaa agtacgcaa aggtccacg 
      tttagaagt tcaccaaga acctgaagg tgcagtcat 
      ttcagccga tcaacttgc tgaatacta ttggagtgg 
      tggaggtcc ttatatgct tcaagaaca agtcgctca 
      atttttggg catcaaatc attcaaacg aaaatatcc 
      caacattca gcgaagagt tgttgatat gcaagagag 
      agattttat tcgcgaaag tggactagc tagcgaagc 
      tatgtgcga tcttggttt gacagctgt caatacggc 
      cgatatttt ggatattat gtattcaga ctttcaaga 
      taaatacga agaatactg caaacatca gcgcgatag 
      gcaggctaa ggatttgac caataagca aaaagcttt 
      gagtttggc ggcaatgca ggagaagac caagatgga 
      tatcacgca gcaggcggt gcaatcggc ggcgagttg 
      gaattctac ttttaataa gacgcgacg agaacagag 
      aaaggcttg tatggattt gcaaacctt aactgttca 
      ctttccaaa aggcaaaat gaagcaaat caacaaacc 
      caaaatcgg gcattatcc agtagcctt agttccagg 
      acaatttac agactatta cagggaatt tacaccaac 
      tgaaataaa taatttacc gctgaatac aatgtgcta 
      caatacttt gatacgagg acgacatca tgaaatgga 
      atgtgatag tcaaactga cggttcagg ttctgagtc 
      ggacacaag cacttcaga ctccgatag ttcttacag 
      tggttcttc agatgcaga agattgcac tctttgcaa 
      accaacacc gaaaagact agctgctgc actctaatg 
      gcatttaag tttgtttta aacattttt ataggacta 
      gtttatagt tttgtacat attttttct ctaaaaaac 
      taaaaataa aacttatgg taattt