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Gene information
General information
TC008484 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11373820..11375336
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC008484
      iBeetle screen iB_01357

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection

      Fertility
      egg production: number <50% of expected -
      ovary without detectable defects - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008484 RA
        MNSFKISLV LVLLAVIDF HQCARYGGS RGRGGGSYT 
RRPHPPPSR RTTSRPRST APTSSRPPN YQSTRRPYP 
TGTTPSSGW NIPPRPGTY PQQSYQGPQ KITIKNKYN 
YNYPGHYSL PVQNPSHPG YFYTPTSYH YSSSDTGST 
ALGFFLGYS LARLTTPTF SHYSFYDGY RPRYDHYTV 
HHYYHNKDA VPQNQEIQS NTIVGCVGD SVTVCPSNT 
TSLCTSNGA LMCVVSATS TVPCTDNRQ VNCVKSVVP 
CVNNTAPEC KNSNQNATT VSIPCISNA KVYGDVKTV 
NNTIVTNST NANSTTSST QYPITTTPV AIVSSSDGT 
TSTYSTKYP ITTTPEPST NPPVRRKRE TAQNFCVTI 
VALPAEKKP TEGELFLKD AKSIVEKFI ESAWNL
      CDS sequence
      • >TC008484 RA
        atgaacagt ttcaaaatt agtttagtg ttggtttta 
ctggcagtg atcgacttt caccaatgt gctcgttac 
ggtggatca agaggtcga ggtggtggt agttacacc 
cgacgaccc catccacca cccagtcga cgcaccact 
agccgacct cgtagcact gccccaacg agttccaga 
ccaccgaat taccaatca actagacgt ccatatccc 
accggaacc acccccagc tctgggtgg aacatacca 
cccagaccc ggcacttac ccccaacaa agttatcaa 
ggaccacaa aaaattacg attaaaaat aaatacaat 
tataattat cccggacat tactcactc ccagtgcag 
aacccgagc caccctggg tacttctac accccaact 
agttatcac tacagtagt agtgatacg ggaagtacc 
gctttggga tttttcctg ggttattcc cttgctcgt 
ttgacaacg cccacgttt tcgcattat tcattttac 
gatgggtac agacctcgg tacgaccat tacaccgtg 
catcattat taccataac aaagacgca gtgccacaa 
aatcaggag attcaatca aacacaatc gttggttgt 
gttggcgat agtgttact gtttgccca tcgaacact 
acatcactt tgcacctca aatggggcc ctcatgtgt 
gttgttagt gcaacttcg accgtacca tgcactgac 
aatcggcaa gtcaactgt gttaaaagt gtggtcccg 
tgtgttaat aacacagca ccggaatgc aaaaattcg 
aaccaaaat gcaacaact gtgagcata ccgtgcatt 
tccaatgcc aaggtttat ggcgatgtt aaaactgtc 
aataatacg attgtaaca aactccaca aatgcgaat 
tcgacgact agttccact caatatccg ataactaca 
acaccagtg gcgattgta agttcaagt gatggtacg 
acatcgact tattcaacg aaatatcct ataactact 
accccggaa ccctccacg aaccctcca gttcggaga 
aaaagggaa acggcgcaa aatttctgt gttactatt 
gttgcactc cctgctgag aagaaacca acggagggg 
gaattattt ttgaaagat gccaaatcg atcgttgag 
aaatttatt gaatctgcc tggaacttg tag
      mRNA sequence
      • >TC008484 RA
        aaggcatca taaggtatt gaactctca aattacgtc 
tactggtac cacctccac acctgtgca ctgaccaat 
accaaacaa gtttcactt cagtgtgtg ttagaccgt 
gaatctgta atctcccaa actagacaa gtgacggtt 
ttccaaata acaagccta aagatgaac agtttcaaa 
attagttta gtgttggtt ttactggca gtgatcgac 
tttcaccaa tgtgctcgt tacggtgga tcaagaggt 
cgaggtggt ggtagttac acccgacga ccccatcca 
ccacccagt cgacgcacc actagccga cctcgtagc 
actgcccca acgagttcc agaccaccg aattaccaa 
tcaactaga cgtccatat cccaccgga accaccccc 
agctctggg tggaacata ccacccaga cccggcact 
tacccccaa caaagttat caaggacca caaaaaatt 
acgattaaa aataaatac aattataat tatcccgga 
cattactca ctcccagtg cagaacccg agccaccct 
gggtacttc tacacccca actagttat cactacagt 
agtagtgat acgggaagt accgctttg ggatttttc 
ctgggttat tcccttgct cgtttgaca acgcccacg 
ttttcgcat tattcattt tacgatggg tacagacct 
cggtacgac cattacacc gtgcatcat tattaccat 
aacaaagac gcagtgcca caaaatcag gagattcaa 
tcaaacaca atcgttggt tgtgttggc gatagtgtt 
actgtttgc ccatcgaac actacatca ctttgcacc 
tcaaatggg gccctcatg tgtgttgtt agtgcaact 
tcgaccgta ccatgcact gacaatcgg caagtcaac 
tgtgttaaa agtgtggtc ccgtgtgtt aataacaca 
gcaccggaa tgcaaaaat tcgaaccaa aatgcaaca 
actgtgagc ataccgtgc atttccaat gccaaggtt 
tatggcgat gttaaaact gtcaataat acgattgta 
acaaactcc acaaatgcg aattcgacg actagttcc 
actcaatat ccgataact acaacacca gtggcgatt 
gtaagttca agtgatggt acgacatcg acttattca 
acgaaatat cctataact actaccccg gaaccctcc 
acgaaccct ccagttcgg agaaaaagg gaaacggcg 
caaaatttc tgtgttact attgttgca ctccctgct 
gagaagaaa ccaacggag ggggaatta tttttgaaa 
gatgccaaa tcgatcgtt gagaaattt attgaatct 
gcctggaac ttgtaggtt tttttttaa cttaatgta 
cagtgttgt tatttcata tttatatgc tcaaaaata 
aaaattatg ttttgta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY