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Gene information
General information
TC008478 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11190351..11191663
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008478
    iBeetle screen iB_01355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008478 RA
      MLKIIQTAT RRGFLKTIN SQRSYLRTF LSQAYYCNE 
      VWDSRLNSP LLQKVNLDG LYYELDQGF QKTRRISPV 
      DVDIFANAL KDDVFIDEL LDLVHKLRL SPDTGNTLN 
      STSHAVVRT LLRNDKVKE LLQVLDDRL NYGVFLDDY 
      TANILMDKF WKSKDFAAG CLVASQMML QEDLKHPVT 
      VNLALLHCY NYLLKPGDW LEMPQPEEP EEEVKIRVN 
      YIRNPFNDE HFDLREPQK IVGKTLAAA TRDRNDSLH 
      NSLRIIGLA LFGKEELVK KEMEARKNT KLIKQVVQL 
      LPDDNPFKA DLTQLELVS EDVQTILEK QLTESIAVT 
      SEKDIANQC SLFKQWENE RLQALEEQK KRLHTLERL 
      AEVERMKKE LSEKEEKLW FFENEEKIE LEIEEGTAA 
      IPTRQTKEK KTDEDYVPP EIIKTHN
    CDS sequence
    • >TC008478 RA
      atgttgaaa attatccaa acagctaca agacggggg 
      tttttgaag acaatcaac tcgcagaga agttacctg 
      cgaacgttt ctatctcaa gcttattac tgtaacgaa 
      gtttgggac tcccggcta aacagcccc cttttacaa 
      aaagtgaac ctcgatggt ctttactat gaactggac 
      caaggtttt caaaagacg cgtcgaatt agccctgtt 
      gatgtcgat attttcgca aatgcgcta aaagacgat 
      gttttcatt gacgaattg ttagatttg gtccacaaa 
      ttgaggtta agtccggac actggtaac actttaaat 
      tcgacttcg cacgctgtg gttcggacc cttttgcga 
      aatgacaag gttaaagag ttgctccaa gttttggac 
      gatcggttg aattacgga gtgtttttg gatgattac 
      actgcgaat attttaatg gataagttc tggaaaagt 
      aaggatttt gcggctggg tgtctcgtc gccagtcag 
      atgatgctc caggaggat ttaaagcac ccagtgacc 
      gtaaatttg gcgctttta cattgctat aattatttg 
      ttgaaacct ggggattgg ctcgagatg ccccaaccg 
      gaagaacct gaagaggaa gtcaaaatt cgggtcaat 
      tatatacgg aatccgttc aatgacgaa catttcgac 
      ttgagggaa cctcagaaa attgtggga aaaacactt 
      gccgcagct actagagac agaaatgat tcgttacac 
      aactcactc agaattatc ggtcttgca ttatttggt 
      aaagaagaa ctagtgaaa aaagaaatg gaggcgaga 
      aaaaacact aaattaatt aagcaagtt gttcagctt 
      ctccctgat gataacccc ttcaaggct gatttaacc 
      caactagaa ttggtgtct gaagacgtg caaacaatt 
      ttagagaaa caactaacc gaaagtatt gccgtgacg 
      tcagaaaag gacatagcc aatcagtgc agtttgttc 
      aaacagtgg gaaaacgaa agactccaa gccttagaa 
      gagcaaaag aaacgtctc cacaccctt gagcgactc 
      gccgaagtt gaacgaatg aagaaagaa ctcagcgaa 
      aaagaagaa aaattatgg ttctttgaa aacgaagaa 
      aagatcgag ctcgaaatc gaagaaggc acagccgcg 
      ataccaacc cgacaaacc aaagaaaag aaaaccgac 
      gaagactac gtcccgcct gagataata aaaacgcac 
      aattag
    mRNA sequence
    • >TC008478 RA
      aatgttgaa aattatcca aacagctac aagacgggg 
      gtttttgaa gacaatcaa ctcgcagag aagttacct 
      gcgaacgtt tctatctca agcttatta ctgtaacga 
      agtttggga ctcccggct aaacagccc ccttttaca 
      aaaagtgaa cctcgatgg tctttacta tgaactgga 
      ccaaggttt tcaaaagac gcgtcgaat tagccctgt 
      tgatgtcga tattttcgc aaatgcgct aaaagacga 
      tgttttcat tgacgaatt gttagattt ggtccacaa 
      attgaggtt aagtccgga cactggtaa cactttaaa 
      ttcgacttc gcacgctgt ggttcggac ccttttgcg 
      aaatgacaa ggttaaaga gttgctcca agttttgga 
      cgatcggtt gaattacgg agtgttttt ggatgatta 
      cactgcgaa tattttaat ggataagtt ctggaaaag 
      taaggattt tgcggctgg gtgtctcgt cgccagtca 
      gatgatgct ccaggagga tttaaagca cccagtgac 
      cgtaaattt ggcgctttt acattgcta taattattt 
      gttgaaacc tggggattg gctcgagat gccccaacc 
      ggaagaacc tgaagagga agtcaaaat tcgggtcaa 
      ttatatacg gaatccgtt caatgacga acatttcga 
      cttgaggga acctcagaa aattgtggg aaaaacact 
      tgccgcagc tactagaga cagaaatga ttcgttaca 
      caactcact cagaattat cggtcttgc attatttgg 
      taaagaaga actagtgaa aaaagaaat ggaggcgag 
      aaaaaacac taaattaat taagcaagt tgttcagct 
      tctccctga tgataaccc cttcaaggc tgatttaac 
      ccaactaga attggtgtc tgaagacgt gcaaacaat 
      tttagagaa acaactaac cgaaagtat tgccgtgac 
      gtcagaaaa ggacatagc caatcagtg cagtttgtt 
      caaacagtg ggaaaacga aagactcca agccttaga 
      agagcaaaa gaaacgtct ccacaccct tgagcgact 
      cgccgaagt tgaacgaat gaagaaaga actcagcga 
      aaaagaaga aaaattatg gttctttga aaacgaaga 
      aaagatcga gctcgaaat cgaagaagg cacagccgc 
      gataccaac ccgacaaac caaagaaaa gaaaaccga 
      cgaagacta cgtcccgcc tgagataat aaaaacgca 
      caattagtt ttttatatt gtaataata acaacaaat 
      ataaatagt aaaaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY