Status
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Gene information
General information
TC032912
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008476,TC008475
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11145827..11152974
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032912
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032912 RA
      MSLIEALEN ECYLKPDFC DNFKNEVKL KIEEFSDWF 
      NSIARLYLL RCQPEESEL FVYLLLNLK KKLKCAISE 
      INKSVSQRK QLFTCNVCE EVVEVEPWQ NVWRVLQDH 
      PHFEETYNQ GFDLVSSSE SSSASIVDD LSEDLNQVL 
      EPEPVNGLN NFKFNFVIK YDSVVEETL YPESFVSAA 
      RKRDHISDH TVQKSGAAR AVCLLCPCS LISRGRVYK 
      VTSYVIKNH TAGQRHLKC ASTPANTSA LRQYHEFWR 
      SQEPNFQAH QVHFLPEIT VHNIVKCHL CQEFVKYTS 
      VIDHIKDKV HKSKLLKIF LSPEGKLNE FYLLEMQVG 
      VYEDVVVNN AAPKRDVKP IKETKEKNP GQPKCAFVK 
      VIFEPNSLH LPPRYKNHS SFFTFCNSV VSCNVCKIN 
      FSNAVLTIW KHISTPLHK KLTGRECPK YNFFCEICN 
      VLIKDENAW DDHFTNGPN RHDSMTPAR KQKVCEYEC 
      KTCLLVIFG DELSLSRHI SSRGGKNRA KDVKLPFSV 
      KRLFRTKAF IQEEAERMQ IEANKVIEN RDKLKECCD 
      DLEQALCGI YPTCKAYPF GSRISGLGN NQSDLDVFM 
      DTGDMYLGE RQQDAQSQE QIVKKASKV FKAFKDQFH 
      SVVSIPTAR TPIVKVHHN FTDLDCDVS FRHGLGVEN 
      TKFLRFCME LQPITQSFI LLLKRWSDY CRLHEHITN 
      YGLALMAVF FLQTGGFLL SVKTVRAYN PTQSLIIDG 
      WETLNYTVP IEKMKEFVK PYTGGVTQL LREFFYYYS 
      KFDYANQVV CPLLGNTIP KLCFNERPQ ELLPIEMKS 
      YVQRIQSEN GEQFQFRGL TPFCVQDPF DLSHNLTKA 
      CQNGTITRL KALCTLTYE LLDSIE
    CDS sequence
    • >TC032912 RA
      atgtccctg atcgaagct ttggaaaac gagtgttat 
      ctgaaacca gacttctgt gataatttt aaaaacgaa 
      gtaaaactc aaaatcgaa gagttttcc gattggttt 
      aatagcata gcacgtttg tatctttta cgttgtcag 
      ccggaagaa agcgaactt tttgtatac ctgcttctt 
      aatcttaag aaaaagctc aaatgtgcg atttcggaa 
      ataaacaag tcggtcagt caacgcaag caattattc 
      acttgtaat gtctgtgag gaagttgtt gaagtggag 
      ccctggcag aacgtgtgg agagtgtta caagaccac 
      ccccatttt gaggaaacg tacaaccaa ggtttcgat 
      ttagtgagt tcgtcagaa tcgtcgagc gcttcaatt 
      gtcgacgac ttgagtgaa gacctcaac caagtgttg 
      gagcctgaa ccggtcaac ggtttaaat aattttaaa 
      tttaatttc gtcataaaa tacgattcg gtggtcgag 
      gaaaccctt tatcctgaa tcgtttgta agcgcagct 
      cgaaaacga gatcatatt tcggaccac actgttcag 
      aaaagtggg gctgcgcgt gcagtttgt ctcctctgt 
      ccgtgcagt ttaatttcc cgcggtaga gtttacaaa 
      gtgacgtcg tatgtgatc aaaaatcac acagcggga 
      caaaggcac ttgaaatgc gcttcaacg cccgcaaat 
      acgagcgct ttgagacag tatcacgag ttttggcga 
      agtcaagag cctaatttt caagcccac caagtgcat 
      ttcctccct gaaatcact gtacacaac atcgtcaaa 
      tgtcacttg tgtcaggaa tttgtaaag tacacttcg 
      gtgattgac cacattaaa gacaaggtg cataaaagt 
      aaactcctg aagattttt ctatcgcct gaaggcaag 
      ttgaacgag ttttacttg cttgagatg caagtgggg 
      gtgtatgag gatgttgtg gtgaacaat gcagcgcct 
      aaaagggat gttaaaccc atcaaagaa acgaaggag 
      aaaaatccc ggacaacca aaatgtgct tttgtcaag 
      gttattttt gaaccaaat agcctacat ttgcccccg 
      agatacaaa aatcattcg agttttttt acgttttgt 
      aattcggtg gtcagttgc aacgtttgc aaaattaat 
      tttagtaat gcagtcctg acgatatgg aaacatatc 
      tcgactcct ctgcataaa aaattgaca gggcgtgag 
      tgccctaaa tacaatttt ttttgcgaa atttgtaat 
      gttttaatc aaggacgaa aacgcctgg gatgaccat 
      ttcaccaat gggccaaac agacacgat agcatgact 
      ccagcccgc aaacaaaaa gtatgtgaa tacgaatgc 
      aaaacttgc ctccttgta attttcggc gatgaatta 
      tccctcagt cgccacatt tcatcacgt ggtgggaaa 
      aaccgagct aaagatgtt aaactccct ttctctgtc 
      aaaagactt ttccgaact aaagctttc atccaagaa 
      gaagccgaa cgaatgcaa atcgaagca aacaaagtc 
      attgagaat cgagataaa ttgaaagaa tgttgtgac 
      gatttggaa caggccttg tgcgggatt tacccaact 
      tgcaaggct tatcctttc ggatcgaga atatcaggc 
      ttggggaat aatcaaagt gatttggat gtgtttatg 
      gacacgggt gatatgtat ttaggtgag aggcaacag 
      gatgcacaa agtcaggaa caaattgtt aagaaagca 
      agtaaggtg ttcaaggcg tttaaggat cagtttcat 
      tctgttgtg tccattcca acggccaga acgcccatt 
      gtcaaagtt catcacaat tttacagac ttggattgt 
      gatgtttcg ttcaggcat ggcttaggc gtcgaaaac 
      accaaattt ctgaggttt tgtatggag ttgcaaccg 
      atcacacaa tcatttatt ttgttgctg aagcggtgg 
      tcggattat tgccgactt cacgaacac ataaccaac 
      tatgggttg gctctaatg gcggttttt ttcctccaa 
      actggtgga tttctcttg tctgttaaa acagtaagg 
      gcttacaat ccaacacaa tcgttaatt attgatggt 
      tgggagact ctaaactac acagttcct atcgaaaaa 
      atgaaagag ttcgttaaa ccctacact ggaggagtg 
      acacaattg ttgcgtgaa tttttttat tactattcg 
      aagtttgac tacgccaat caagtggtt tgtccattg 
      ttgggtaat actattccg aaattgtgt ttcaacgaa 
      agaccccag gaattatta ccaattgaa atgaagagt 
      tatgttcag agaatacaa agtgaaaac ggggagcaa 
      ttccaattc cgtggtttg actccgttt tgtgtccaa 
      gatccgttc gatttgagt cacaattta acgaaagct 
      tgccaaaat ggcacaatc accagattg aaagctttg 
      tgtactttg acgtacgag ttattggac tctattgaa 
      taa
    mRNA sequence
    • >TC032912 RA
      tgttatttt ttagtgtga cgtagttgg aaaatatca 
      gaggttata ataacaaat ttaacacaa atatgttat 
      tatttgcaa aattatcgt aactggagt gctatgtag 
      ttatttttg ggtgttact ttattgtta cgtttattc 
      cgattgaag tgcaaaaag tgaggttat cccaccatg 
      tccctgatc gaagctttg gaaaacgag tgttatctg 
      aaaccagac ttctgtgat aattttaaa aacgaagta 
      aaactcaaa atcgaagag ttttccgat tggtttaat 
      agcatagca cgtttgtat cttttacgt tgtcagccg 
      gaagaaagc gaacttttt gtatacctg cttcttaat 
      cttaagaaa aagctcaaa tgtgcgatt tcggaaata 
      aacaagtcg gtcagtcaa cgcaagcaa ttattcact 
      tgtaatgtc tgtgaggaa gttgttgaa gtggagccc 
      tggcagaac gtgtggaga gtgttacaa gaccacccc 
      cattttgag gaaacgtac aaccaaggt ttcgattta 
      gtgagttcg tcagaatcg tcgagcgct tcaattgtc 
      gacgacttg agtgaagac ctcaaccaa gtgttggag 
      cctgaaccg gtcaacggt ttaaataat tttaaattt 
      aatttcgtc ataaaatac gattcggtg gtcgaggaa 
      accctttat cctgaatcg tttgtaagc gcagctcga 
      aaacgagat catatttcg gaccacact gttcagaaa 
      agtggggct gcgcgtgca gtttgtctc ctctgtccg 
      tgcagttta atttcccgc ggtagagtt tacaaagtg 
      acgtcgtat gtgatcaaa aatcacaca gcgggacaa 
      aggcacttg aaatgcgct tcaacgccc gcaaatacg 
      agcgctttg agacagtat cacgagttt tggcgaagt 
      caagagcct aattttcaa gcccaccaa gtgcatttc 
      ctccctgaa atcactgta cacaacatc gtcaaatgt 
      cacttgtgt caggaattt gtaaagtac acttcggtg 
      attgaccac attaaagac aaggtgcat aaaagtaaa 
      ctcctgaag atttttcta tcgcctgaa ggcaagttg 
      aacgagttt tacttgctt gagatgcaa gtgggggtg 
      tatgaggat gttgtggtg aacaatgca gcgcctaaa 
      agggatgtt aaacccatc aaagaaacg aaggagaaa 
      aatcccgga caaccaaaa tgtgctttt gtcaaggtt 
      atttttgaa ccaaatagc ctacatttg cccccgaga 
      tacaaaaat cattcgagt ttttttacg ttttgtaat 
      tcggtggtc agttgcaac gtttgcaaa attaatttt 
      agtaatgca gtcctgacg atatggaaa catatctcg 
      actcctctg cataaaaaa ttgacaggg cgtgagtgc 
      cctaaatac aattttttt tgcgaaatt tgtaatgtt 
      ttaatcaag gacgaaaac gcctgggat gaccatttc 
      accaatggg ccaaacaga cacgatagc atgactcca 
      gcccgcaaa caaaaagta tgtgaatac gaatgcaaa 
      acttgcctc cttgtaatt ttcggcgat gaattatcc 
      ctcagtcgc cacatttca tcacgtggt gggaaaaac 
      cgagctaaa gatgttaaa ctccctttc tctgtcaaa 
      agacttttc cgaactaaa gctttcatc caagaagaa 
      gccgaacga atgcaaatc gaagcaaac aaagtcatt 
      gagaatcga gataaattg aaagaatgt tgtgacgat 
      ttggaacag gccttgtgc gggatttac ccaacttgc 
      aaggcttat cctttcgga tcgagaata tcaggcttg 
      gggaataat caaagtgat ttggatgtg tttatggac 
      acgggtgat atgtattta ggtgagagg caacaggat 
      gcacaaagt caggaacaa attgttaag aaagcaagt 
      aaggtgttc aaggcgttt aaggatcag tttcattct 
      gttgtgtcc attccaacg gccagaacg cccattgtc 
      aaagttcat cacaatttt acagacttg gattgtgat 
      gtttcgttc aggcatggc ttaggcgtc gaaaacacc 
      aaatttctg aggttttgt atggagttg caaccgatc 
      acacaatca tttattttg ttgctgaag cggtggtcg 
      gattattgc cgacttcac gaacacata accaactat 
      gggttggct ctaatggcg gtttttttc ctccaaact 
      ggtggattt ctcttgtct gttaaaaca gtaagggct 
      tacaatcca acacaatcg ttaattatt gatggttgg 
      gagactcta aactacaca gttcctatc gaaaaaatg 
      aaagagttc gttaaaccc tacactgga ggagtgaca 
      caattgttg cgtgaattt ttttattac tattcgaag 
      tttgactac gccaatcaa gtggtttgt ccattgttg 
      ggtaatact attccgaaa ttgtgtttc aacgaaaga 
      ccccaggaa ttattacca attgaaatg aagagttat 
      gttcagaga atacaaagt gaaaacggg gagcaattc 
      caattccgt ggtttgact ccgttttgt gtccaagat 
      ccgttcgat ttgagtcac aatttaacg aaagcttgc 
      caaaatggc acaatcacc agattgaaa gctttgtgt 
      actttgacg tacgagtta ttggactct attgaataa 
      tattttaag tgcctgtga gtggcaaaa tgtgatgtt 
      tgtttggtg tatagtttt taataatga cgaagtaaa 
      ggcaaacat agaatatat tttctcttc acct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY