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Gene information
General information
TC008473 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 11135817..11137716
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008473
    iBeetle screen iB_01353
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008473 RA
      MAHSVNTTP LLQEVDSSD NENDILENG VVIRDNKPL 
      FKSAEPKDK YYMAYIIFY LLGMVTLLP WNFFITADD 
      YWLYKFRNV SNNDTSVSK RTPLQTCFT SYISVASAV 
      PNLVFLILN TALTHRISL HKRVIGSLT FMLGLFIMT 
      LVFVTTNTD KWQDTFFIV TITTIVLLN VCSAVLSGS 
      IFGVVGRFC PIYITATLG GQALGGIFA ALAEIASLS 
      IGASSVHSA FVYFLIGNL TILSSIICY IILTKTVFF 
      NFHLYERRI AQNEFENEL LRPRIIDHK MIVKKIWTH 
      GLSMFMVFA ITLSVYPSV TVLVESEGK GQGRRWNDV 
      YFVPTIAYL LFSVGDYLG RILAGRIQK PKKGYILLI 
      LSTARFVFI PLLMLCNAQ PRSHWAVVF DHDYEYISI 
      LFLCALSNG YLANITAIC APRVVESHE KETASSMMT 
      VFMGVGLAL GSGISLYMV KLL
    CDS sequence
    • >TC008473 RA
      atggcacat tccgtcaat accactcca cttctccaa 
      gaagttgac tcttcggac aatgagaac gatatttta 
      gaaaatggg gttgttata cgggataat aagccattg 
      ttcaagtcg gctgaaccc aaagacaag tactacatg 
      gcgtatatc atattttac ttgctgggg atggtgaca 
      ttgcttccc tggaatttt ttcataaca gctgatgat 
      tactggttg tacaagttt cgtaatgtt agcaataac 
      gatacttca gtaagtaag cgaacccct ttacaaacg 
      tgtttcaca tcatacata agtgtggct tcagcagtc 
      ccaaattta gtgtttttg attttaaat accgcactg 
      acccatcga atctcactt cataaacgc gtcattgga 
      tcattaact tttatgtta ggtttgttt ataatgaca 
      ctggttttt gtcactact aacacagat aagtggcaa 
      gacaccttt tttattgtc acaatcaca acaattgtt 
      ttgttgaat gtttgtagt gctgtgtta tctggcagt 
      attttcggg gtggttgga cggttttgc cccatttat 
      attacggcc actttgggc ggacaagcc ttaggtgga 
      atttttgca gccttggct gaaatcgcc tcattgtca 
      attggagcg tcctcagtt cacagcgcg ttcgtttat 
      ttccttatc ggcaattta acaatcctc agttcaatt 
      atctgttac atcatctta acaaaaaca gtatttttt 
      aatttccat ttgtatgaa agaagaatt gctcaaaat 
      gaatttgaa aacgaacta cttagacca cgcattatt 
      gatcataaa atgattgtg aagaaaata tggacccat 
      ggcttgtct atgtttatg gtgtttgcc atcacacta 
      tcagtttat ccaagcgtt acggtttta gtggaatct 
      gaagggaaa ggacaaggg cgcagatgg aacgatgtt 
      tattttgtg cccactatc gcatatctc ttatttagt 
      gtgggggat tacctgggc cggattttg gcgggaagg 
      attcaaaag cccaagaaa ggttacatt ttgctcatt 
      ttgagtaca gcaagattt gtttttatc ccactcttg 
      atgttgtgt aacgcccag ccacggtca cactgggct 
      gttgttttt gaccatgat tacgaatac atttcaata 
      ttatttctc tgtgcttta tccaacggt tatttggct 
      aatattaca gctatttgt gctccaagg gttgtggaa 
      tcacatgaa aaggaaact gcgtcttct atgatgaca 
      gtttttatg ggcgttgga cttgctcta gggtctgga 
      attagtctc tacatggtg aaattactg taa
    mRNA sequence
    • >TC008473 RA
      tcaaaaaat gttgacatg tcagtctta tcactcaaa 
      actgactta acgtttaat tttatcaca atgtaaaat 
      aacagagaa caaacgtgc atttgccga acaagaatg 
      gcacattcc gtcaatacc actccactt ctccaagaa 
      gttgactct tcggacaat gagaacgat attttagaa 
      aatggggtt gttatacgg gataataag ccattgttc 
      aagtcggct gaacccaaa gacaagtac tacatggcg 
      tatatcata ttttacttg ctggggatg gtgacattg 
      cttccctgg aattttttc ataacagct gatgattac 
      tggttgtac aagtttcgt aatgttagc aataacgat 
      acttcagta agtaagcga accccttta caaacgtgt 
      ttcacatca tacataagt gtggcttca gcagtccca 
      aatttagtg tttttgatt ttaaatacc gcactgacc 
      catcgaatc tcacttcat aaacgcgtc attggatca 
      ttaactttt atgttaggt ttgtttata atgacactg 
      gtttttgtc actactaac acagataag tggcaagac 
      acctttttt attgtcaca atcacaaca attgttttg 
      ttgaatgtt tgtagtgct gtgttatct ggcagtatt 
      ttcggggtg gttggacgg ttttgcccc atttatatt 
      acggccact ttgggcgga caagcctta ggtggaatt 
      tttgcagcc ttggctgaa atcgcctca ttgtcaatt 
      ggagcgtcc tcagttcac agcgcgttc gtttatttc 
      cttatcggc aatttaaca atcctcagt tcaattatc 
      tgttacatc atcttaaca aaaacagta ttttttaat 
      ttccatttg tatgaaaga agaattgct caaaatgaa 
      tttgaaaac gaactactt agaccacgc attattgat 
      cataaaatg attgtgaag aaaatatgg acccatggc 
      ttgtctatg tttatggtg tttgccatc acactatca 
      gtttatcca agcgttacg gttttagtg gaatctgaa 
      gggaaagga caagggcgc agatggaac gatgtttat 
      tttgtgccc actatcgca tatctctta tttagtgtg 
      ggggattac ctgggccgg attttggcg ggaaggatt 
      caaaagccc aagaaaggt tacattttg ctcattttg 
      agtacagca agatttgtt tttatccca ctcttgatg 
      ttgtgtaac gcccagcca cggtcacac tgggctgtt 
      gtttttgac catgattac gaatacatt tcaatatta 
      tttctctgt gctttatcc aacggttat ttggctaat 
      attacagct atttgtgct ccaagggtt gtggaatca 
      catgaaaag gaaactgcg tcttctatg atgacagtt 
      tttatgggc gttggactt gctctaggg tctggaatt 
      agtctctac atggtgaaa ttactgtaa tggtactta 
      acattcttt tttattcct actgccatt aattgtgat 
      atttatacg tgtgaccca aattaactc attaggggt 
      agaattacc ttcctagac gtaaataaa cgatagtta 
      agaaa