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Gene information
General information
TC008465 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11034776..11037012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008465
    iBeetle screen iB_01352

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008465 RA
      MVFMRLYLS RKSRTIRLA CGVLILFVI LYLLASYQR 
      PDGFDLESP IAARLSHKE RPKLVSDLG NFEPRDSQE 
      HEGPGEGGK PHHLRQDQQ NDADQSESE YGMNVACSD 
      EISLDRTIL DTRLSECKH WNYPENLPS TSVIIVFHN 
      EGWSVLLRT VHSVINRSP PKILKEVLL VDDFSDKEN 
      LKTRLETYI ERFNGKVRL IRNAQREGL IRTRSRGAK 
      EATGEVIVF LDAHCEVNT NWLPPLLAP IYRDRSVMT 
      VPVIDGIDH KTFEYRPVY GEDRHFRGI FEWGMLYKE 
      NEVPQKELN TRKHNSEPY KSPTHAGGL FAINREYFL 
      ELGAYDPGL LVWGGENFE LSFKIWQCG GSIEWVPCS 
      RVGHVYRSF MPYNFGKLA QKKKGPLIT INYKRVIET 
      WFDDKYKEF FYTREPMAR FLDMGDISE QLALKKRLN 
      CKSFEWFME NVAYDVLDK YPELPPNIH FGELRSVAV 
      SKCLDTLGH GPPSLMAIQ HCHGYGNNQ LIRLNAKGQ 
      LGVGERCIE ADAQGIKLA FCRMGTVDG PWLYDENTH 
      TILHRVHKK CIALHPQTS HLSLMPCDI NNAYQQWIF 
      KEIRPKW
    CDS sequence
    • >TC008465 RA
      atggttttt atgcggcta tatttaagt aggaaatca 
      cggacgatt cgactagct tgcggcgtt ttaatcctc 
      tttgttatc ctctacttg ttagcttcg tatcaacgg 
      ccagacggc ttcgatttg gagtcgccc attgcggct 
      cgtttatcg cacaaggag cgccccaaa ctagtctcc 
      gacttgggc aactttgag ccccgggac tcccaagag 
      cacgagggg cccggcgaa gggggcaaa ccccaccac 
      ttgcgccaa gaccagcaa aacgacgcc gatcagtcc 
      gagtccgaa tatggcatg aacgttgct tgttccgac 
      gaaatctcc ctagaccgt acaatcctg gacacgaga 
      ttgtccgaa tgcaagcac tggaactac cctgagaac 
      ctcccctcg acttccgtg atcatagtt ttccacaat 
      gagggctgg tctgttctc ctccgaaca gtccactcg 
      gtcatcaac cgctcgccg cccaaaatc ctcaaagag 
      gtgcttctt gtcgacgac ttcagcgac aaggagaac 
      ctcaaaacg cgcctcgag acctacatt gagcgcttc 
      aatggtaaa gttcgcttg atccggaac gctcaacgt 
      gaaggcctc atccgcaca cgctcacgg ggggccaag 
      gaagccacg ggggaagtt atcgtgttt ttggacgcg 
      cattgcgag gttaacact aactggctt ccgccgctc 
      cttgccccc atttaccgt gaccggagc gttatgaca 
      gtcccggtt attgacggg attgatcat aaaactttc 
      gagtatcgg ccggtttat ggcgaggat cgacatttt 
      cggggcatt tttgaatgg gggatgctg tacaaggag 
      aatgaagtg cctcaaaaa gagctcaac acgcgtaaa 
      cataacagc gaaccgtac aaaagtcca acacatgcc 
      ggggggttg tttgccatc aacagggag tatttccta 
      gagttgggg gcatatgac cctggtttg ttggtttgg 
      ggtggggaa aatttcgaa ttgagtttt aagatatgg 
      caatgtggg gggagtatc gagtgggta ccatgttcc 
      cgagttggc catgtgtat cgcagcttc atgccttat 
      aacttcggt aagttggct cagaagaaa aagggaccc 
      ttgattacg ataaactac aagcgagtg attgaaacg 
      tggtttgat gataagtac aaggagttt ttctatacg 
      cgcgaacca atggctcgg ttccttgat atgggggat 
      attagcgaa cagttagca ctaaagaaa cggctcaac 
      tgtaaaagc tttgaatgg tttatggaa aatgtagcc 
      tacgatgtt ttggataag tatccagaa ttaccacct 
      aatatccac ttcggggag cttcgctct gtagctgtt 
      agcaagtgt ttggatact ttgggccat gggcctccg 
      tccctcatg gccatacaa cactgccat ggttatggt 
      aacaatcaa ctgatcagg ttaaatgct aaaggtcag 
      ttgggtgtg ggcgaaagg tgtatagaa gccgatgcc 
      caagggatc aaactggca ttttgtcgc atgggtaca 
      gtcgatggg ccatggttg tatgacgaa aacacccat 
      acgatactg catagggta cataagaaa tgtatagct 
      ttacatcca cagacatca catctttcg ttaatgccg 
      tgtgatatt aataatgcc taccaacaa tggattttt 
      aaggagatt cgacccaag tggtaa
    mRNA sequence
    • >TC008465 RA
      aatgaatgt caaaaatcg ccggttctg tttacgtgt 
      tgacatttc ccacgactg attaaaatt tcaatttac 
      agtaaaacc aacgaatat ttatcgcag ttccgatga 
      aaagttaac gccggactt tagactagt caccaatca 
      ccaataaaa tggttttta tgcggctat atttaagta 
      ggaaatcac ggacgattc gactagctt gcggcgttt 
      taatcctct ttgttatcc tctacttgt tagcttcgt 
      atcaacggc cagacggct tcgatttgg agtcgccca 
      ttgcggctc gtttatcgc acaaggagc gccccaaac 
      tagtctccg acttgggca actttgagc cccgggact 
      cccaagagc acgaggggc ccggcgaag ggggcaaac 
      cccaccact tgcgccaag accagcaaa acgacgccg 
      atcagtccg agtccgaat atggcatga acgttgctt 
      gttccgacg aaatctccc tagaccgta caatcctgg 
      acacgagat tgtccgaat gcaagcact ggaactacc 
      ctgagaacc tcccctcga cttccgtga tcatagttt 
      tccacaatg agggctggt ctgttctcc tccgaacag 
      tccactcgg tcatcaacc gctcgccgc ccaaaatcc 
      tcaaagagg tgcttcttg tcgacgact tcagcgaca 
      aggagaacc tcaaaacgc gcctcgaga cctacattg 
      agcgcttca atggtaaag ttcgcttga tccggaacg 
      ctcaacgtg aaggcctca tccgcacac gctcacggg 
      gggccaagg aagccacgg gggaagtta tcgtgtttt 
      tggacgcgc attgcgagg ttaacacta actggcttc 
      cgccgctcc ttgccccca tttaccgtg accggagcg 
      ttatgacag tcccggtta ttgacggga ttgatcata 
      aaactttcg agtatcggc cggtttatg gcgaggatc 
      gacattttc ggggcattt ttgaatggg ggatgctgt 
      acaaggaga atgaagtgc ctcaaaaag agctcaaca 
      cgcgtaaac ataacagcg aaccgtaca aaagtccaa 
      cacatgccg gggggttgt ttgccatca acagggagt 
      atttcctag agttggggg catatgacc ctggtttgt 
      tggtttggg gtggggaaa atttcgaat tgagtttta 
      agatatggc aatgtgggg ggagtatcg agtgggtac 
      catgttccc gagttggcc atgtgtatc gcagcttca 
      tgccttata acttcggta agttggctc agaagaaaa 
      agggaccct tgattacga taaactaca agcgagtga 
      ttgaaacgt ggtttgatg ataagtaca aggagtttt 
      tctatacgc gcgaaccaa tggctcggt tccttgata 
      tgggggata ttagcgaac agttagcac taaagaaac 
      ggctcaact gtaaaagct ttgaatggt ttatggaaa 
      atgtagcct acgatgttt tggataagt atccagaat 
      taccaccta atatccact tcggggagc ttcgctctg 
      tagctgtta gcaagtgtt tggatactt tgggccatg 
      ggcctccgt ccctcatgg ccatacaac actgccatg 
      gttatggta acaatcaac tgatcaggt taaatgcta 
      aaggtcagt tgggtgtgg gcgaaaggt gtatagaag 
      ccgatgccc aagggatca aactggcat tttgtcgca 
      tgggtacag tcgatgggc catggttgt atgacgaaa 
      acacccata cgatactgc atagggtac ataagaaat 
      gtatagctt tacatccac agacatcac atctttcgt 
      taatgccgt gtgatatta ataatgcct accaacaat 
      ggattttta aggagattc gacccaagt ggtaacaac 
      ttccagatt ttttacttt attttttac atatcatcg 
      attcatctg caataagta cttacgttt ttgatcaat 
      catactgtg ccttatatc actaggacg tgtaaatag 
      ttttgtaag aatgtttta taaatcggg actggttga 
      ctagttaac aaattgtat gtcgtagtt atttattaa 
      ggagttgtg ttaaatttt gtaatacga taaaagtag 
      gtgcttgta aactgtctc gttgttttt gtacataat 
      gaaatacac gaacaaaac attt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY