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Gene information
General information
TC032940
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008424,TC008425
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 10069971..10076072
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032940
    iBeetle screen iB_01350
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032940 RA
      MRTYRDDSS SDEDLDYGY ISTLKQEGY ARDLLYKNT 
      KDILLNRIY CCVSVQELF NVIERNSHF YRCEDITQT 
      VFVLYDLMR IFCDVAVAN RKSLTEAKD EFLNNLLAN 
      GTFQKLLQI TEEKIHTFD SNTLSYCLL YLRKLGLDV 
      EIKPVQLMN ETIRKKLEK EFLIAPASR YLETIFDEV 
      NLRSYFMAQ ELIPLVLNE IDRRESPNG LHDLTVSLN 
      KLHRVVTNE TLDKYKEKV ENFIQSGLL TSSDYKVVL 
      QIIAFLNYP KWREKNSVL ISKCILLIK NNLNDLNME 
      EIMMLYETF FKIQEPGEI LNSLQRCSS KFFHQLDET 
      PTAQKLTLF SIIVYFASP NLRTDFQNI LKKYVNECN 
      DYDNLMILR KLLSYVKVT DERLCSLFW DKVLKFVNK 
      DLDIIKLTQ NYINFSNDT GNYRHFEFE TTVLTLIGD 
      LVQEANYKF FPQKLSNLL SFVLLYGED HSLLQHLVA 
      KLEESWEQL QYIDCLRLA FTLKVFNTV ENKYKKLPY 
      AKRINRVLN ELINKLAAD SVNDLWKNN ALLKACIYM 
      QRADAIATN RLFNNFKCF DHISSKNIE NIYYCFLMA 
      NSLIPEVLD NLTEYIVTY KNHVLGFNV ARVVYLCYS 
      LGYQPKKSS EFFETAINV IIRDQERMT GLSLIYTAV 
      ALCFFNQLP NSLIKSIFN EEFMDKLDS ELQKCYYKD 
      KYPQKVRYN LMELNRAVC LDYPESNVP WFHQKYVDE 
      NIKGQAVKI TLFGKKVRE YLKKIISSS GLAENVITA 
      YGYKVDFVV SLNSRDEVV EESKAVKRL ALLLLPEFA 
      CTKIVTRLK GPFQMKKRH LEILGYKVI FVKSSDWIE 
      LYYAEDRVD FLKQLIWPN CNTTNLNKN CGFTMPKEK 
      FGYPVMSIN PRAILRLRI LNENNRVIY QLPDDFVDK 
      YLCKIVDKV IEVIRDPDY KHEPEEATH VITGDFFVS 
      FKPEVLEFL RLHQLELIY PLMMQACTT SLTRNAHDV 
      EMCDIQPII ATKYKVWIK GSSVEYFVQ NRARPRRVN 
      TQRWVRSRI MIHSKVRNL DAPPLILNL TNCN
    CDS sequence
    • >TC032940 RA
      atgaggacg tatcgcgat gacagttcc tcggatgaa 
      gacttggat tacggttac ataagcaca ctgaaacag 
      gagggctac gcccgggat ttgttgtac aaaaacacc 
      aaagatatt ttactcaat cgcatttat tgttgtgtc 
      tcagttcaa gagttgttt aatgtgata gagcgcaat 
      tcgcatttt tacagatgt gaggacatt acacagaca 
      gtgtttgtg ttgtatgat ttaatgcgg attttttgt 
      gacgttgct gttgcaaac agaaaaagt ttaacagag 
      gccaaagac gagttttta aataatctt ctcgccaat 
      ggtactttc caaaagttg ctccaaatc actgaggag 
      aaaatccac acttttgat agcaatacg ttgagttat 
      tgtctactc tatttgaga aagttgggc ttggatgtt 
      gagataaaa ccggtccaa ttaatgaat gaaacaatt 
      aggaaaaaa cttgaaaaa gagtttttg atagctcct 
      gcttcacga tacttggaa acgattttt gatgaagtt 
      aacttaagg tcgtacttt atggcgcaa gaactgatc 
      cctctagtc cttaatgaa attgatcgt cgtgagtct 
      ccaaacggg ctccatgat cttactgta agtctcaat 
      aaattacac agagttgta acaaacgaa acattagat 
      aagtataaa gaaaaagtt gagaatttt attcaaagt 
      ggattgtta acgtcgtca gactataaa gtggttcta 
      caaatcata gcgttttta aattacccc aaatggagg 
      gaaaaaaac tcagtccta atctccaaa tgcatctta 
      ctgataaaa aataattta aatgacttg aatatggaa 
      gaaattatg atgttgtat gagacgttt tttaaaatc 
      caagaacca ggcgaaatc ctcaatagt ctccaacgt 
      tgctcgtct aagtttttc caccaactg gacgaaacc 
      ccgacagcg caaaaacta acacttttc tcgataata 
      gtttatttc gcgtctcca aatttgcga accgacttt 
      caaaacata ttgaaaaaa tatgtaaac gagtgcaac 
      gattacgac aatttgatg attttgcgc aaattattg 
      tcatacgtg aaagtcaca gacgagaga ctttgtagt 
      ttgttttgg gacaaagtt ttaaaattt gtaaacaaa 
      gatttagac ataattaaa ctaacccag aactacatt 
      aattttagt aacgacacg gggaattac agacatttc 
      gaattcgaa actactgtt ttaacatta attggtgat 
      ttagttcaa gaggcgaat tataagttc ttcccccaa 
      aaattatcg aatctgtta agttttgtt ttattgtac 
      ggtgaggat cacagtttg cttcagcat ttggtggcg 
      aagttggaa gagagttgg gagcaactt caatacatt 
      gattgtttg aggctcgct ttcacactg aaagttttc 
      aatacggtg gaaaataag tacaagaaa ttgccatac 
      gcgaagagg attaatcgt gtccttaac gaattaatc 
      aataaattg gcggcagat agcgttaac gacttgtgg 
      aaaaataac gcgcttctc aaggcttgt atttacatg 
      cagagagcc gatgcaatt gctacaaac aggttattt 
      aacaatttt aaatgtttc gatcacatt tcgtcgaaa 
      aatatcgaa aatatttac tattgtttc cttatggcc 
      aactctctc ataccggaa gtcttagat aatttaact 
      gaatatatc gttacttac aaaaaccac gttttgggt 
      tttaatgtc gcacgggtg gtttacctg tgttactct 
      ttgggttat caaccgaaa aaatcaagc gaatttttc 
      gaaactgcc atcaatgtt ataattaga gatcaggaa 
      cggatgacg ggcctatcg ctgatttac acagctgtg 
      gctttatgt ttcttcaac caactgcca aattcgtta 
      ataaagtca atttttaac gaggaattt atggacaag 
      ctcgacagt gaattgcaa aagtgttac tacaaggat 
      aaatatcca caaaaagtt cgttacaat ttaatggag 
      ctcaatcgt gcggtttgc ttggactat cccgaaagc 
      aacgttccg tggttccac caaaaatac gttgatgaa 
      aatatcaaa ggccaggca gtgaaaatc acacttttc 
      gggaaaaaa gtgagagaa tatttgaaa aaaatcatc 
      tcaagttcg ggcctcgcc gaaaatgtt atcacagct 
      tatggttac aaagtcgat tttgtggtc agtttaaat 
      tcgcgagac gaggtcgtt gaggagtca aaagctgtg 
      aaaagactg gccttgttg ttactgccc gagtttgcg 
      tgcactaaa attgtgact cggctaaaa ggtcctttc 
      cagatgaaa aaacgccac ttggaaatc ctggggtac 
      aaagtgatt tttgttaaa tcaagcgac tggattgaa 
      ctatattac gccgaagat agagtggat tttttgaaa 
      caattaatt tggccgaat tgtaacacg acgaattta 
      aataaaaac tgtggtttc acgatgcct aaagagaag 
      ttcggttat ccggttatg agcattaac cctagggcc 
      attttgcgc cttaggata ctcaatgaa aacaaccga 
      gtcatttac caactgcct gacgatttt gtcgataaa 
      tatctttgt aaaatcgtc gataaggtc atagaggta 
      attcgggac ccggattac aaacacgaa cccgaagaa 
      gcgacgcat gtaatcacc ggtgacttt ttcgtttct 
      ttcaaaccc gaggtgttg gagttctta agactgcat 
      caacttgaa ctgatttat cctctgatg atgcaagct 
      tgcaccact tcgctcaca cgcaatgca cacgacgtc 
      gaaatgtgc gatattcag ccgattatc gccacgaaa 
      tacaaggtg tggatcaaa gggagctcg gttgaatat 
      tttgtgcaa aacagggct cgtccgaga agagttaac 
      actcagaga tgggtgcgc tcccggatt atgatccac 
      agcaaggtg aggaacttg gacgcacct cctcttata 
      ctgaattta acaaactgt aattaa
    mRNA sequence
    • >TC032940 RA
      actcacgtg tcaacttgt caagctttt aacctcaaa 
      aagtaacct cacttttga ccatgtaaa atatgaggt 
      tcagtaatt tatttttga tgtattcga gacgtgcag 
      tgtgtgtaa gatcattag tgacgccct tacgttatt 
      ttcaagtaa agaaccaaa aatgtgact tggctcgac 
      atcaattat ccacttgtg cccccttga aaatgagga 
      cgtatcgcg atgacagtt cctcggatg aagacttgg 
      attacggtt acataagca cactgaaac aggagggct 
      acgcccggg atttgttgt acaaaaaca ccaaagata 
      ttttactca atcgcattt attgttgtg tctcagttc 
      aagagttgt ttaatgtga tagagcgca attcgcatt 
      tttacagat gtgaggaca ttacacaga cagtgtttg 
      tgttgtatg atttaatgc ggatttttt gtgacgttg 
      ctgttgcaa acagaaaaa gtttaacag aggccaaag 
      acgagtttt taaataatc ttctcgcca atggtactt 
      tccaaaagt tgctccaaa tcactgagg agaaaatcc 
      acacttttg atagcaata cgttgagtt attgtctac 
      tctatttga gaaagttgg gcttggatg ttgagataa 
      aaccggtcc aattaatga atgaaacaa ttaggaaaa 
      aacttgaaa aagagtttt tgatagctc ctgcttcac 
      gatacttgg aaacgattt ttgatgaag ttaacttaa 
      ggtcgtact ttatggcgc aagaactga tccctctag 
      tccttaatg aaattgatc gtcgtgagt ctccaaacg 
      ggctccatg atcttactg taagtctca ataaattac 
      acagagttg taacaaacg aaacattag ataagtata 
      aagaaaaag ttgagaatt ttattcaaa gtggattgt 
      taacgtcgt cagactata aagtggttc tacaaatca 
      tagcgtttt taaattacc ccaaatgga gggaaaaaa 
      actcagtcc taatctcca aatgcatct tactgataa 
      aaaataatt taaatgact tgaatatgg aagaaatta 
      tgatgttgt atgagacgt tttttaaaa tccaagaac 
      caggcgaaa tcctcaata gtctccaac gttgctcgt 
      ctaagtttt tccaccaac tggacgaaa ccccgacag 
      cgcaaaaac taacacttt tctcgataa tagtttatt 
      tcgcgtctc caaatttgc gaaccgact ttcaaaaca 
      tattgaaaa aatatgtaa acgagtgca acgattacg 
      acaatttga tgattttgc gcaaattat tgtcatacg 
      tgaaagtca cagacgaga gactttgta gtttgtttt 
      gggacaaag ttttaaaat ttgtaaaca aagatttag 
      acataatta aactaaccc agaactaca ttaatttta 
      gtaacgaca cggggaatt acagacatt tcgaattcg 
      aaactactg ttttaacat taattggtg atttagttc 
      aagaggcga attataagt tcttccccc aaaaattat 
      cgaatctgt taagttttg ttttattgt acggtgagg 
      atcacagtt tgcttcagc atttggtgg cgaagttgg 
      aagagagtt gggagcaac ttcaataca ttgattgtt 
      tgaggctcg ctttcacac tgaaagttt tcaatacgg 
      tggaaaata agtacaaga aattgccat acgcgaaga 
      ggattaatc gtgtcctta acgaattaa tcaataaat 
      tggcggcag atagcgtta acgacttgt ggaaaaata 
      acgcgcttc tcaaggctt gtatttaca tgcagagag 
      ccgatgcaa ttgctacaa acaggttat ttaacaatt 
      ttaaatgtt tcgatcaca tttcgtcga aaaatatcg 
      aaaatattt actattgtt tccttatgg ccaactctc 
      tcataccgg aagtcttag ataatttaa ctgaatata 
      tcgttactt acaaaaacc acgttttgg gttttaatg 
      tcgcacggg tggtttacc tgtgttact ctttgggtt 
      atcaaccga aaaaatcaa gcgaatttt tcgaaactg 
      ccatcaatg ttataatta gagatcagg aacggatga 
      cgggcctat cgctgattt acacagctg tggctttat 
      gtttcttca accaactgc caaattcgt taataaagt 
      caattttta acgaggaat ttatggaca agctcgaca 
      gtgaattgc aaaagtgtt actacaagg ataaatatc 
      cacaaaaag ttcgttaca atttaatgg agctcaatc 
      gtgcggttt gcttggact atcccgaaa gcaacgttc 
      cgtggttcc accaaaaat acgttgatg aaaatatca 
      aaggccagg cagtgaaaa tcacacttt tcgggaaaa 
      aagtgagag aatatttga aaaaaatca tctcaagtt 
      cgggcctcg ccgaaaatg ttatcacag cttatggtt 
      acaaagtcg attttgtgg tcagtttaa attcgcgag 
      acgaggtcg ttgaggagt caaaagctg tgaaaagac 
      tggccttgt tgttactgc ccgagtttg cgtgcacta 
      aaattgtga ctcggctaa aaggtcctt tccagatga 
      aaaaacgcc acttggaaa tcctggggt acaaagtga 
      tttttgtta aatcaagcg actggattg aactatatt 
      acgccgaag atagagtgg attttttga aacaattaa 
      tttggccga attgtaaca cgacgaatt taaataaaa 
      actgtggtt tcacgatgc ctaaagaga agttcggtt 
      atccggtta tgagcatta accctaggg ccattttgc 
      gccttagga tactcaatg aaaacaacc gagtcattt 
      accaactgc ctgacgatt ttgtcgata aatatcttt 
      gtaaaatcg tcgataagg tcatagagg taattcggg 
      acccggatt acaaacacg aacccgaag aagcgacgc 
      atgtaatca ccggtgact ttttcgttt ctttcaaac 
      ccgaggtgt tggagttct taagactgc atcaacttg 
      aactgattt atcctctga tgatgcaag cttgcacca 
      cttcgctca cacgcaatg cacacgacg tcgaaatgt 
      gcgatattc agccgatta tcgccacga aatacaagg 
      tgtggatca aagggagct cggttgaat attttgtgc 
      aaaacaggg ctcgtccga gaagagtta acactcaga 
      gatgggtgc gctcccgga ttatgatcc acagcaagg 
      tgaggaact tggacgcac ctcctctta tactgaatt 
      taacaaact gtaattaat taatcttga ttaaatgaa 
      tttacttga a