| General information | |||
| TC008415 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9994191..10000487 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008415 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01349 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
- >TC008415 RA
MALQKNKKG SSPWFQCEK CLIHVTNKD IDKHSSDCP PNSNSYNFI QNQVLFGTL DIKVNEEIK GVVDKDSLV FLSQSAIQL CGLSIGEWG LLEFEDLPP VAKVVWPTT EKSLTGVLL TKHAMKLCQ GEVNQNVKV STIPEQLSE AYRVTLVAL NKPKSLEFT PELTNRLQK TFVEKIFCV NNRLSLMFF GKVLMFEIK SIESEQLAD DFGNLTIKG SHFYKITTK TSWTLFSDY SDFKEKTLN NDLLSTIGG LDDEIADIK EAINACLST KKSYGLKHC KSILLYGNS GTGKTLLAR AISREFKTH IIEINASDL YSKYSGNVE ETIKNLFDE AIEHAPTII ILDEIDILC PTRTQRMTD SEKRVSAML LTMLDNLNS SSVFLLATT NKLESIDPV FRRFGRLER EIEISTPNP KNRQKILSK LLSQVVHNL SEADLGEIA LNTHGFVGA DLLALCSRA GLIASKREA EKITFDDFK AALKHVRPS AMREVQVEV ANVRWGDIG GLQNLKLIL RQAVEWPLR HPESFLRLG VTPPKGVLM FGPPGCSKT MIAKALATE SGLNFLSIK GPELFSKWV GESEKAVRE VFRKARQVA PSVIFFDEI DALGGERSS GSSTSVQER VLAQLLTEL DGVSPLGDV TVLAATNRP DRIDKALLR PGRLDRIVY VPLPDDDTR REIFKLKLG KMPVCNVDV EELVRLTPG YSGAEVNAV CHEAAMMAL EDSLDARFV EKRHFEKAL TIVKPRTPD SLIQLYQDY LNKKI
- >TC008415 RA
atggccctc caaaagaac aagaaaggt tcttccccg tggttccag tgtgaaaag tgtcttatc catgtcacg aacaaggac atcgataag cattcaagt gattgccct ccgaactca aacagttac aatttcatc caaaaccaa gtccttttc ggaaccctc gacattaaa gtgaatgaa gaaattaaa ggagtggtt gacaaagac agtttggtg tttttaagt caaagtgca attcagtta tgtggactt tcaatagga gaatggggg ttattggaa ttcgaggat ttgccacct gtggctaaa gtggtttgg cccacgact gagaaaagt ttaacgggg gttttacta acaaaacat gctatgaaa ttgtgtcaa ggggaggtg aatcaaaat gttaaagtc tcaacaatt ccggagcaa ttatccgaa gcttacaga gttacttta gtggcgtta aataaacca aaatcgctc gaatttacc cccgagttg accaacagg ttgcaaaaa accttcgta gagaaaata ttttgtgta aacaataga ctcagtttg atgtttttt ggtaaagtg ttaatgttt gaaatcaaa tcaatcgaa agcgaacaa ctagccgac gattttggg aatttgaca ataaaagga agtcatttt tataaaatt acgaccaag actagctgg actttattc agtgattac agcgatttt aaggaaaaa acacttaat aacgactta ctctcaaca attgggggg ttggatgac gaaatcgca gacataaaa gaggcaata aatgcgtgt ttaagtaca aaaaaatca tacggcctc aaacattgc aaatcgatt cttttgtat ggaaattca ggaactggt aaaacttta ctagctcga gctatttcc cgcgaattt aagacacac attattgaa ataaatgcc tcagatctg tacagcaaa tacagcgga aatgttgaa gaaacgatc aaaaatttg tttgatgag gcaatcgaa cacgcaccc actatcata attttggac gaaattgat attttatgt cccacaaga acgcagaga atgaccgat tcggaaaaa cgagtctct gccatgtta ctaacaatg ttggataat ttaaactct agtagtgtt ttccttctt gctacgaca aataaactg gaatcgata gatccagtg tttcgccgt tttggtcgc cttgaacgt gaaatcgaa atcagtact cccaacccc aagaaccgg caaaaaatc ctctcgaaa ttattatca caagtcgtc cataattta tcagaggcg gatttgggc gaaattgcg ttaaatacc cacggtttc gttggggct gatttgctc gcgttgtgc tccagggcc ggacttatc gcctccaaa cgcgaggct gaaaaaatc acttttgat gattttaaa gcggcttta aagcacgtc cgaccaagc gcaatgagg gaagttcaa gtggaagtg gcaaatgtg cgttggggt gatattggg gggcttcag aatcttaaa ttgatactc agacaggct gtagagtgg cctttgaga caccctgag agttttcta cgactaggg gtgacccca ccgaagggg gttttgatg ttcgggccc cccggatgc tccaaaacg atgattgcc aaggctttg gctaccgaa agtgggctc aattttctc tcaattaaa gggccggag ttgtttagt aagtgggtg ggcgagtcg gaaaaggca gttcgggag gtgtttcgc aaagcgcgc caagtggcc ccttctgtg atttttttc gacgaaatt gatgcttta ggaggcgaa agaagttca gggtctagc actagtgtg caagaaagg gttttggcc cagttactg acagagttg gacggagtt tcgcctcta ggtgatgtc acagttctg gcagcgaca aataggcca gaccgtatt gacaaggcg ctgctaagg ccaggccgt ttggacagg attgtttat gtccctttg cctgatgat gacacgaga cgagaaatc tttaaatta aaactcgga aaaatgccg gtttgtaac gtggatgtt gaggaattg gtccgtttg acaccggga tactcgggg gctgaggtg aatgcggtg tgtcatgaa gctgcgatg atggcttta gaagacagt ctggacgcg agatttgtg gagaaaagg cattttgaa aaagctttg acgatagta aagccaagg acaccggat agcttaatt caattgtac caagattat ttaaataaa aaaatataa
- >TC008415 RA
accttgagg tcgtccttg tgtttctaa cctcaaaat ttttaaata aaaacccac gatttaact gaaatggcc ctccaaaag aacaagaaa ggttcttcc ccgtggttc cagtgtgaa aagtgtctt atccatgtc acgaacaag gacatcgat aagcattca agtgattgc cctccgaac tcaaacagt tacaatttc atccaaaac caagtcctt ttcggaacc ctcgacatt aaagtgaat gaagaaatt aaaggagtg gttgacaaa gacagtttg gtgttttta agtcaaagt gcaattcag ttatgtgga ctttcaata ggagaatgg gggttattg gaattcgag gatttgcca cctgtggct aaagtggtt tggcccacg actgagaaa agtttaacg ggggtttta ctaacaaaa catgctatg aaattgtgt caaggggag gtgaatcaa aatgttaaa gtctcaaca attccggag caattatcc gaagcttac agagttact ttagtggcg ttaaataaa ccaaaatcg ctcgaattt acccccgag ttgaccaac aggttgcaa aaaaccttc gtagagaaa atattttgt gtaaacaat agactcagt ttgatgttt tttggtaaa gtgttaatg tttgaaatc aaatcaatc gaaagcgaa caactagcc gacgatttt gggaatttg acaataaaa ggaagtcat ttttataaa attacgacc aagactagc tggacttta ttcagtgat tacagcgat tttaaggaa aaaacactt aataacgac ttactctca acaattggg gggttggat gacgaaatc gcagacata aaagaggca ataaatgcg tgtttaagt acaaaaaaa tcatacggc ctcaaacat tgcaaatcg attcttttg tatggaaat tcaggaact ggtaaaact ttactagct cgagctatt tcccgcgaa tttaagaca cacattatt gaaataaat gcctcagat ctgtacagc aaatacagc ggaaatgtt gaagaaacg atcaaaaat ttgtttgat gaggcaatc gaacacgca cccactatc ataattttg gacgaaatt gatatttta tgtcccaca agaacgcag agaatgacc gattcggaa aaacgagtc tctgccatg ttactaaca atgttggat aatttaaac tctagtagt gttttcctt cttgctacg acaaataaa ctggaatcg atagatcca gtgtttcgc cgttttggt cgccttgaa cgtgaaatc gaaatcagt actcccaac cccaagaac cggcaaaaa atcctctcg aaattatta tcacaagtc gtccataat ttatcagag gcggatttg ggcgaaatt gcgttaaat acccacggt ttcgttggg gctgatttg ctcgcgttg tgctccagg gccggactt atcgcctcc aaacgcgag gctgaaaaa atcactttt gatgatttt aaagcggct ttaaagcac gtccgacca agcgcaatg agggaagtt caagtggaa gtggcaaat gtgcgttgg ggtgatatt ggggggctt cagaatctt aaattgata ctcagacag gctgtagag tggcctttg agacaccct gagagtttt ctacgacta ggggtgacc ccaccgaag ggggttttg atgttcggg ccccccgga tgctccaaa acgatgatt gccaaggct ttggctacc gaaagtggg ctcaatttt ctctcaatt aaagggccg gagttgttt agtaagtgg gtgggcgag tcggaaaag gcagttcgg gaggtgttt cgcaaagcg cgccaagtg gccccttct gtgattttt ttcgacgaa attgatgct ttaggaggc gaaagaagt tcagggtct agcactagt gtgcaagaa agggttttg gcccagtta ctgacagag ttggacgga gtttcgcct ctaggtgat gtcacagtt ctggcagcg acaaatagg ccagaccgt attgacaag gcgctgcta aggccaggc cgtttggac aggattgtt tatgtccct ttgcctgat gatgacacg agacgagaa atctttaaa ttaaaactc ggaaaaatg ccggtttgt aacgtggat gttgaggaa ttggtccgt ttgacaccg ggatactcg ggggctgag gtgaatgcg gtgtgtcat gaagctgcg atgatggct ttagaagac agtctggac gcgagattt gtggagaaa aggcatttt gaaaaagct ttgacgata gtaaagcca aggacaccg gatagctta attcaattg taccaagat tatttaaat aaaaaaata taaatttaa aattgtttt atttgccgc gaacgccag tttaaatta aaccaggga atcgatttg ctgttgaat gtgtgttgt ggcgaaaac acaatggat cagtttagt taaacgctc aagcagaat aaatgtctc ggaaaatt
