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Gene information
General information
TC008415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9994191..10000487
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008415
    iBeetle screen iB_01349

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008415 RA
      MALQKNKKG SSPWFQCEK CLIHVTNKD IDKHSSDCP 
PNSNSYNFI QNQVLFGTL DIKVNEEIK GVVDKDSLV 
FLSQSAIQL CGLSIGEWG LLEFEDLPP VAKVVWPTT 
EKSLTGVLL TKHAMKLCQ GEVNQNVKV STIPEQLSE 
AYRVTLVAL NKPKSLEFT PELTNRLQK TFVEKIFCV 
NNRLSLMFF GKVLMFEIK SIESEQLAD DFGNLTIKG 
SHFYKITTK TSWTLFSDY SDFKEKTLN NDLLSTIGG 
LDDEIADIK EAINACLST KKSYGLKHC KSILLYGNS 
GTGKTLLAR AISREFKTH IIEINASDL YSKYSGNVE 
ETIKNLFDE AIEHAPTII ILDEIDILC PTRTQRMTD 
SEKRVSAML LTMLDNLNS SSVFLLATT NKLESIDPV 
FRRFGRLER EIEISTPNP KNRQKILSK LLSQVVHNL 
SEADLGEIA LNTHGFVGA DLLALCSRA GLIASKREA 
EKITFDDFK AALKHVRPS AMREVQVEV ANVRWGDIG 
GLQNLKLIL RQAVEWPLR HPESFLRLG VTPPKGVLM 
FGPPGCSKT MIAKALATE SGLNFLSIK GPELFSKWV 
GESEKAVRE VFRKARQVA PSVIFFDEI DALGGERSS 
GSSTSVQER VLAQLLTEL DGVSPLGDV TVLAATNRP 
DRIDKALLR PGRLDRIVY VPLPDDDTR REIFKLKLG 
KMPVCNVDV EELVRLTPG YSGAEVNAV CHEAAMMAL 
EDSLDARFV EKRHFEKAL TIVKPRTPD SLIQLYQDY 
LNKKI
    CDS sequence
    • >TC008415 RA
      atggccctc caaaagaac aagaaaggt tcttccccg 
tggttccag tgtgaaaag tgtcttatc catgtcacg 
aacaaggac atcgataag cattcaagt gattgccct 
ccgaactca aacagttac aatttcatc caaaaccaa 
gtccttttc ggaaccctc gacattaaa gtgaatgaa 
gaaattaaa ggagtggtt gacaaagac agtttggtg 
tttttaagt caaagtgca attcagtta tgtggactt 
tcaatagga gaatggggg ttattggaa ttcgaggat 
ttgccacct gtggctaaa gtggtttgg cccacgact 
gagaaaagt ttaacgggg gttttacta acaaaacat 
gctatgaaa ttgtgtcaa ggggaggtg aatcaaaat 
gttaaagtc tcaacaatt ccggagcaa ttatccgaa 
gcttacaga gttacttta gtggcgtta aataaacca 
aaatcgctc gaatttacc cccgagttg accaacagg 
ttgcaaaaa accttcgta gagaaaata ttttgtgta 
aacaataga ctcagtttg atgtttttt ggtaaagtg 
ttaatgttt gaaatcaaa tcaatcgaa agcgaacaa 
ctagccgac gattttggg aatttgaca ataaaagga 
agtcatttt tataaaatt acgaccaag actagctgg 
actttattc agtgattac agcgatttt aaggaaaaa 
acacttaat aacgactta ctctcaaca attgggggg 
ttggatgac gaaatcgca gacataaaa gaggcaata 
aatgcgtgt ttaagtaca aaaaaatca tacggcctc 
aaacattgc aaatcgatt cttttgtat ggaaattca 
ggaactggt aaaacttta ctagctcga gctatttcc 
cgcgaattt aagacacac attattgaa ataaatgcc 
tcagatctg tacagcaaa tacagcgga aatgttgaa 
gaaacgatc aaaaatttg tttgatgag gcaatcgaa 
cacgcaccc actatcata attttggac gaaattgat 
attttatgt cccacaaga acgcagaga atgaccgat 
tcggaaaaa cgagtctct gccatgtta ctaacaatg 
ttggataat ttaaactct agtagtgtt ttccttctt 
gctacgaca aataaactg gaatcgata gatccagtg 
tttcgccgt tttggtcgc cttgaacgt gaaatcgaa 
atcagtact cccaacccc aagaaccgg caaaaaatc 
ctctcgaaa ttattatca caagtcgtc cataattta 
tcagaggcg gatttgggc gaaattgcg ttaaatacc 
cacggtttc gttggggct gatttgctc gcgttgtgc 
tccagggcc ggacttatc gcctccaaa cgcgaggct 
gaaaaaatc acttttgat gattttaaa gcggcttta 
aagcacgtc cgaccaagc gcaatgagg gaagttcaa 
gtggaagtg gcaaatgtg cgttggggt gatattggg 
gggcttcag aatcttaaa ttgatactc agacaggct 
gtagagtgg cctttgaga caccctgag agttttcta 
cgactaggg gtgacccca ccgaagggg gttttgatg 
ttcgggccc cccggatgc tccaaaacg atgattgcc 
aaggctttg gctaccgaa agtgggctc aattttctc 
tcaattaaa gggccggag ttgtttagt aagtgggtg 
ggcgagtcg gaaaaggca gttcgggag gtgtttcgc 
aaagcgcgc caagtggcc ccttctgtg atttttttc 
gacgaaatt gatgcttta ggaggcgaa agaagttca 
gggtctagc actagtgtg caagaaagg gttttggcc 
cagttactg acagagttg gacggagtt tcgcctcta 
ggtgatgtc acagttctg gcagcgaca aataggcca 
gaccgtatt gacaaggcg ctgctaagg ccaggccgt 
ttggacagg attgtttat gtccctttg cctgatgat 
gacacgaga cgagaaatc tttaaatta aaactcgga 
aaaatgccg gtttgtaac gtggatgtt gaggaattg 
gtccgtttg acaccggga tactcgggg gctgaggtg 
aatgcggtg tgtcatgaa gctgcgatg atggcttta 
gaagacagt ctggacgcg agatttgtg gagaaaagg 
cattttgaa aaagctttg acgatagta aagccaagg 
acaccggat agcttaatt caattgtac caagattat 
ttaaataaa aaaatataa
    mRNA sequence
    • >TC008415 RA
      accttgagg tcgtccttg tgtttctaa cctcaaaat 
ttttaaata aaaacccac gatttaact gaaatggcc 
ctccaaaag aacaagaaa ggttcttcc ccgtggttc 
cagtgtgaa aagtgtctt atccatgtc acgaacaag 
gacatcgat aagcattca agtgattgc cctccgaac 
tcaaacagt tacaatttc atccaaaac caagtcctt 
ttcggaacc ctcgacatt aaagtgaat gaagaaatt 
aaaggagtg gttgacaaa gacagtttg gtgttttta 
agtcaaagt gcaattcag ttatgtgga ctttcaata 
ggagaatgg gggttattg gaattcgag gatttgcca 
cctgtggct aaagtggtt tggcccacg actgagaaa 
agtttaacg ggggtttta ctaacaaaa catgctatg 
aaattgtgt caaggggag gtgaatcaa aatgttaaa 
gtctcaaca attccggag caattatcc gaagcttac 
agagttact ttagtggcg ttaaataaa ccaaaatcg 
ctcgaattt acccccgag ttgaccaac aggttgcaa 
aaaaccttc gtagagaaa atattttgt gtaaacaat 
agactcagt ttgatgttt tttggtaaa gtgttaatg 
tttgaaatc aaatcaatc gaaagcgaa caactagcc 
gacgatttt gggaatttg acaataaaa ggaagtcat 
ttttataaa attacgacc aagactagc tggacttta 
ttcagtgat tacagcgat tttaaggaa aaaacactt 
aataacgac ttactctca acaattggg gggttggat 
gacgaaatc gcagacata aaagaggca ataaatgcg 
tgtttaagt acaaaaaaa tcatacggc ctcaaacat 
tgcaaatcg attcttttg tatggaaat tcaggaact 
ggtaaaact ttactagct cgagctatt tcccgcgaa 
tttaagaca cacattatt gaaataaat gcctcagat 
ctgtacagc aaatacagc ggaaatgtt gaagaaacg 
atcaaaaat ttgtttgat gaggcaatc gaacacgca 
cccactatc ataattttg gacgaaatt gatatttta 
tgtcccaca agaacgcag agaatgacc gattcggaa 
aaacgagtc tctgccatg ttactaaca atgttggat 
aatttaaac tctagtagt gttttcctt cttgctacg 
acaaataaa ctggaatcg atagatcca gtgtttcgc 
cgttttggt cgccttgaa cgtgaaatc gaaatcagt 
actcccaac cccaagaac cggcaaaaa atcctctcg 
aaattatta tcacaagtc gtccataat ttatcagag 
gcggatttg ggcgaaatt gcgttaaat acccacggt 
ttcgttggg gctgatttg ctcgcgttg tgctccagg 
gccggactt atcgcctcc aaacgcgag gctgaaaaa 
atcactttt gatgatttt aaagcggct ttaaagcac 
gtccgacca agcgcaatg agggaagtt caagtggaa 
gtggcaaat gtgcgttgg ggtgatatt ggggggctt 
cagaatctt aaattgata ctcagacag gctgtagag 
tggcctttg agacaccct gagagtttt ctacgacta 
ggggtgacc ccaccgaag ggggttttg atgttcggg 
ccccccgga tgctccaaa acgatgatt gccaaggct 
ttggctacc gaaagtggg ctcaatttt ctctcaatt 
aaagggccg gagttgttt agtaagtgg gtgggcgag 
tcggaaaag gcagttcgg gaggtgttt cgcaaagcg 
cgccaagtg gccccttct gtgattttt ttcgacgaa 
attgatgct ttaggaggc gaaagaagt tcagggtct 
agcactagt gtgcaagaa agggttttg gcccagtta 
ctgacagag ttggacgga gtttcgcct ctaggtgat 
gtcacagtt ctggcagcg acaaatagg ccagaccgt 
attgacaag gcgctgcta aggccaggc cgtttggac 
aggattgtt tatgtccct ttgcctgat gatgacacg 
agacgagaa atctttaaa ttaaaactc ggaaaaatg 
ccggtttgt aacgtggat gttgaggaa ttggtccgt 
ttgacaccg ggatactcg ggggctgag gtgaatgcg 
gtgtgtcat gaagctgcg atgatggct ttagaagac 
agtctggac gcgagattt gtggagaaa aggcatttt 
gaaaaagct ttgacgata gtaaagcca aggacaccg 
gatagctta attcaattg taccaagat tatttaaat 
aaaaaaata taaatttaa aattgtttt atttgccgc 
gaacgccag tttaaatta aaccaggga atcgatttg 
ctgttgaat gtgtgttgt ggcgaaaac acaatggat 
cagtttagt taaacgctc aagcagaat aaatgtctc 
ggaaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY