Status
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Gene information
General information
TC008415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9994191..10000487
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008415
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01349

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008415 RA
      MALQKNKKG SSPWFQCEK CLIHVTNKD IDKHSSDCP 
      PNSNSYNFI QNQVLFGTL DIKVNEEIK GVVDKDSLV 
      FLSQSAIQL CGLSIGEWG LLEFEDLPP VAKVVWPTT 
      EKSLTGVLL TKHAMKLCQ GEVNQNVKV STIPEQLSE 
      AYRVTLVAL NKPKSLEFT PELTNRLQK TFVEKIFCV 
      NNRLSLMFF GKVLMFEIK SIESEQLAD DFGNLTIKG 
      SHFYKITTK TSWTLFSDY SDFKEKTLN NDLLSTIGG 
      LDDEIADIK EAINACLST KKSYGLKHC KSILLYGNS 
      GTGKTLLAR AISREFKTH IIEINASDL YSKYSGNVE 
      ETIKNLFDE AIEHAPTII ILDEIDILC PTRTQRMTD 
      SEKRVSAML LTMLDNLNS SSVFLLATT NKLESIDPV 
      FRRFGRLER EIEISTPNP KNRQKILSK LLSQVVHNL 
      SEADLGEIA LNTHGFVGA DLLALCSRA GLIASKREA 
      EKITFDDFK AALKHVRPS AMREVQVEV ANVRWGDIG 
      GLQNLKLIL RQAVEWPLR HPESFLRLG VTPPKGVLM 
      FGPPGCSKT MIAKALATE SGLNFLSIK GPELFSKWV 
      GESEKAVRE VFRKARQVA PSVIFFDEI DALGGERSS 
      GSSTSVQER VLAQLLTEL DGVSPLGDV TVLAATNRP 
      DRIDKALLR PGRLDRIVY VPLPDDDTR REIFKLKLG 
      KMPVCNVDV EELVRLTPG YSGAEVNAV CHEAAMMAL 
      EDSLDARFV EKRHFEKAL TIVKPRTPD SLIQLYQDY 
      LNKKI
    CDS sequence
    • >TC008415 RA
      atggccctc caaaagaac aagaaaggt tcttccccg 
      tggttccag tgtgaaaag tgtcttatc catgtcacg 
      aacaaggac atcgataag cattcaagt gattgccct 
      ccgaactca aacagttac aatttcatc caaaaccaa 
      gtccttttc ggaaccctc gacattaaa gtgaatgaa 
      gaaattaaa ggagtggtt gacaaagac agtttggtg 
      tttttaagt caaagtgca attcagtta tgtggactt 
      tcaatagga gaatggggg ttattggaa ttcgaggat 
      ttgccacct gtggctaaa gtggtttgg cccacgact 
      gagaaaagt ttaacgggg gttttacta acaaaacat 
      gctatgaaa ttgtgtcaa ggggaggtg aatcaaaat 
      gttaaagtc tcaacaatt ccggagcaa ttatccgaa 
      gcttacaga gttacttta gtggcgtta aataaacca 
      aaatcgctc gaatttacc cccgagttg accaacagg 
      ttgcaaaaa accttcgta gagaaaata ttttgtgta 
      aacaataga ctcagtttg atgtttttt ggtaaagtg 
      ttaatgttt gaaatcaaa tcaatcgaa agcgaacaa 
      ctagccgac gattttggg aatttgaca ataaaagga 
      agtcatttt tataaaatt acgaccaag actagctgg 
      actttattc agtgattac agcgatttt aaggaaaaa 
      acacttaat aacgactta ctctcaaca attgggggg 
      ttggatgac gaaatcgca gacataaaa gaggcaata 
      aatgcgtgt ttaagtaca aaaaaatca tacggcctc 
      aaacattgc aaatcgatt cttttgtat ggaaattca 
      ggaactggt aaaacttta ctagctcga gctatttcc 
      cgcgaattt aagacacac attattgaa ataaatgcc 
      tcagatctg tacagcaaa tacagcgga aatgttgaa 
      gaaacgatc aaaaatttg tttgatgag gcaatcgaa 
      cacgcaccc actatcata attttggac gaaattgat 
      attttatgt cccacaaga acgcagaga atgaccgat 
      tcggaaaaa cgagtctct gccatgtta ctaacaatg 
      ttggataat ttaaactct agtagtgtt ttccttctt 
      gctacgaca aataaactg gaatcgata gatccagtg 
      tttcgccgt tttggtcgc cttgaacgt gaaatcgaa 
      atcagtact cccaacccc aagaaccgg caaaaaatc 
      ctctcgaaa ttattatca caagtcgtc cataattta 
      tcagaggcg gatttgggc gaaattgcg ttaaatacc 
      cacggtttc gttggggct gatttgctc gcgttgtgc 
      tccagggcc ggacttatc gcctccaaa cgcgaggct 
      gaaaaaatc acttttgat gattttaaa gcggcttta 
      aagcacgtc cgaccaagc gcaatgagg gaagttcaa 
      gtggaagtg gcaaatgtg cgttggggt gatattggg 
      gggcttcag aatcttaaa ttgatactc agacaggct 
      gtagagtgg cctttgaga caccctgag agttttcta 
      cgactaggg gtgacccca ccgaagggg gttttgatg 
      ttcgggccc cccggatgc tccaaaacg atgattgcc 
      aaggctttg gctaccgaa agtgggctc aattttctc 
      tcaattaaa gggccggag ttgtttagt aagtgggtg 
      ggcgagtcg gaaaaggca gttcgggag gtgtttcgc 
      aaagcgcgc caagtggcc ccttctgtg atttttttc 
      gacgaaatt gatgcttta ggaggcgaa agaagttca 
      gggtctagc actagtgtg caagaaagg gttttggcc 
      cagttactg acagagttg gacggagtt tcgcctcta 
      ggtgatgtc acagttctg gcagcgaca aataggcca 
      gaccgtatt gacaaggcg ctgctaagg ccaggccgt 
      ttggacagg attgtttat gtccctttg cctgatgat 
      gacacgaga cgagaaatc tttaaatta aaactcgga 
      aaaatgccg gtttgtaac gtggatgtt gaggaattg 
      gtccgtttg acaccggga tactcgggg gctgaggtg 
      aatgcggtg tgtcatgaa gctgcgatg atggcttta 
      gaagacagt ctggacgcg agatttgtg gagaaaagg 
      cattttgaa aaagctttg acgatagta aagccaagg 
      acaccggat agcttaatt caattgtac caagattat 
      ttaaataaa aaaatataa
    mRNA sequence
    • >TC008415 RA
      accttgagg tcgtccttg tgtttctaa cctcaaaat 
      ttttaaata aaaacccac gatttaact gaaatggcc 
      ctccaaaag aacaagaaa ggttcttcc ccgtggttc 
      cagtgtgaa aagtgtctt atccatgtc acgaacaag 
      gacatcgat aagcattca agtgattgc cctccgaac 
      tcaaacagt tacaatttc atccaaaac caagtcctt 
      ttcggaacc ctcgacatt aaagtgaat gaagaaatt 
      aaaggagtg gttgacaaa gacagtttg gtgttttta 
      agtcaaagt gcaattcag ttatgtgga ctttcaata 
      ggagaatgg gggttattg gaattcgag gatttgcca 
      cctgtggct aaagtggtt tggcccacg actgagaaa 
      agtttaacg ggggtttta ctaacaaaa catgctatg 
      aaattgtgt caaggggag gtgaatcaa aatgttaaa 
      gtctcaaca attccggag caattatcc gaagcttac 
      agagttact ttagtggcg ttaaataaa ccaaaatcg 
      ctcgaattt acccccgag ttgaccaac aggttgcaa 
      aaaaccttc gtagagaaa atattttgt gtaaacaat 
      agactcagt ttgatgttt tttggtaaa gtgttaatg 
      tttgaaatc aaatcaatc gaaagcgaa caactagcc 
      gacgatttt gggaatttg acaataaaa ggaagtcat 
      ttttataaa attacgacc aagactagc tggacttta 
      ttcagtgat tacagcgat tttaaggaa aaaacactt 
      aataacgac ttactctca acaattggg gggttggat 
      gacgaaatc gcagacata aaagaggca ataaatgcg 
      tgtttaagt acaaaaaaa tcatacggc ctcaaacat 
      tgcaaatcg attcttttg tatggaaat tcaggaact 
      ggtaaaact ttactagct cgagctatt tcccgcgaa 
      tttaagaca cacattatt gaaataaat gcctcagat 
      ctgtacagc aaatacagc ggaaatgtt gaagaaacg 
      atcaaaaat ttgtttgat gaggcaatc gaacacgca 
      cccactatc ataattttg gacgaaatt gatatttta 
      tgtcccaca agaacgcag agaatgacc gattcggaa 
      aaacgagtc tctgccatg ttactaaca atgttggat 
      aatttaaac tctagtagt gttttcctt cttgctacg 
      acaaataaa ctggaatcg atagatcca gtgtttcgc 
      cgttttggt cgccttgaa cgtgaaatc gaaatcagt 
      actcccaac cccaagaac cggcaaaaa atcctctcg 
      aaattatta tcacaagtc gtccataat ttatcagag 
      gcggatttg ggcgaaatt gcgttaaat acccacggt 
      ttcgttggg gctgatttg ctcgcgttg tgctccagg 
      gccggactt atcgcctcc aaacgcgag gctgaaaaa 
      atcactttt gatgatttt aaagcggct ttaaagcac 
      gtccgacca agcgcaatg agggaagtt caagtggaa 
      gtggcaaat gtgcgttgg ggtgatatt ggggggctt 
      cagaatctt aaattgata ctcagacag gctgtagag 
      tggcctttg agacaccct gagagtttt ctacgacta 
      ggggtgacc ccaccgaag ggggttttg atgttcggg 
      ccccccgga tgctccaaa acgatgatt gccaaggct 
      ttggctacc gaaagtggg ctcaatttt ctctcaatt 
      aaagggccg gagttgttt agtaagtgg gtgggcgag 
      tcggaaaag gcagttcgg gaggtgttt cgcaaagcg 
      cgccaagtg gccccttct gtgattttt ttcgacgaa 
      attgatgct ttaggaggc gaaagaagt tcagggtct 
      agcactagt gtgcaagaa agggttttg gcccagtta 
      ctgacagag ttggacgga gtttcgcct ctaggtgat 
      gtcacagtt ctggcagcg acaaatagg ccagaccgt 
      attgacaag gcgctgcta aggccaggc cgtttggac 
      aggattgtt tatgtccct ttgcctgat gatgacacg 
      agacgagaa atctttaaa ttaaaactc ggaaaaatg 
      ccggtttgt aacgtggat gttgaggaa ttggtccgt 
      ttgacaccg ggatactcg ggggctgag gtgaatgcg 
      gtgtgtcat gaagctgcg atgatggct ttagaagac 
      agtctggac gcgagattt gtggagaaa aggcatttt 
      gaaaaagct ttgacgata gtaaagcca aggacaccg 
      gatagctta attcaattg taccaagat tatttaaat 
      aaaaaaata taaatttaa aattgtttt atttgccgc 
      gaacgccag tttaaatta aaccaggga atcgatttg 
      ctgttgaat gtgtgttgt ggcgaaaac acaatggat 
      cagtttagt taaacgctc aagcagaat aaatgtctc 
      ggaaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY