Status
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Gene information
General information
TC008407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9857888..9866072
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008407
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008407 RA
      MLTKCVLVI LLLSAYNQA QEINTSDVE ILSKNDPQP 
      PLVNQVDEN PNQGGNDDR PDQPENERE KSVPNNTTK 
      LDTSDDAVE VFESPDVVL RKGPNGKYM FDYGDNYDP 
      MTSRYAPQE DSQRGRAGP PYVITEARL KEIREHFMY 
      PYYDRGGNA DNEGDYQKA IQTSTPQVH KNLNFQLPF 
      FGFRYNYTR VSLNGYLEF SDPPQNYET YPLVFPVKD 
      WPKKNDPAF IGIFYSKCR IGNLRVEDI DQRMPGVYF 
      RLERDLRER NDQMGVEIR ERLKWDIRE GVIGADNFN 
      PKHAIIATW KNVSFAGGF ANALYKTNT FQLVLATDE 
      VYTYAMFNY LNLDWTSHT EAGGDTING EGGIPAYVG 
      FNAGNGTRS YQYVPYSQN SVIRDLTGT GFGNEKKGR 
      HYFRIDENI YLGSCNKDI DGANLDLFF APESGNMLG 
      GTIVNITGP CFKPTDQII CKFDTADEV YGVVVSSNR 
      AICIQPPLL VEGYVKLEI AIGPGTYKW KGKYYVETP 
      ATATQKIFF DDMSYNEKS PAEIGFTWE KQNLTTNEN 
      ANIRISLWG YRETSVRPD FLYITDLAE NVQNTGSYK 
      IIPSTYKNR NNEKLIDIQ FGFIQINLT ESIPVTNKN 
      GQGEVKITP LIWSRPIPL GWYFGPQWE RKYGTNWPK 
      YLCDNWLKD DRYLKNFAS ELPQCPCTL QQALADKGR 
      FMPDFDCDK DANPKCYFH RQAVHCVKT GSPSLQGAE 
      QQCCYDKNH YLMLSYDQK WGSYPRRSH NLGYLPWYE 
      STKVPTLSQ WYNDMIPRF VCCLWQEET AVGCETLRF 
      ERRPTQDCV AYQAPAVAG VYGDPHILT FDDLPYTFN 
      GKGEFVLVK SESVRNRLE IQARFEQMP MNAYGEVRA 
      TQLTSVVAR GNSTTIIEI RRRPQEARW RYRLDVIAD 
      RRRIYFDRP SLKFQHFPG VTVYTPTYI FNQSEVIVM 
      FDSGAGLEV VENNGYLTA RVYLPWSFI NQTRGLFGN 
      WSFDIMDDF TLPNGDKKG VVTNLNDMS SVYTDFGMK 
      WMLDDVGDD QKGAALFFR EHGKTASSY NNKTFVPEF 
      RMSIEELIP ENRSEYRSR AYDLCTATN YECLYDYAM 
      TYNRDVAHF SANYKASIK ELKETNRDN IVSCGVLET 
      PRFGRKDTF LFVPGTKVT FECSQDFIL VGDQRRECL 
      STGEWNIPE YGYTECLRH QEYSSRKAA ITGGIVLAV 
      IIPILLIIA FCAFSLFKR LANDKSSWQ YNAVKSSRP 
      LSPMSDDGS SLRSPVPSD RSSTSSLGK KRRSYDKVY 
      RTHEPLEGL PETEFEEKP WDPNEFEPD SKPTSPTSV 
      IYTQPFSKP DLVKFQSNP NLDRFTYNS DDYTLPVKK 
      NRMSRQQEY SQRQAGIAS GIVLAVIIP LVLLFVYIA 
      YRFFEKRQQ EKDEEEQDA LNYEAKKRE AQEIAARKL 
      TAGEHFDDD TASSIVSGG KETTID
    CDS sequence
    • >TC008407 RA
      atgttgaca aagtgtgtg ttggtgatt cttctacta 
      agtgcatac aaccaagct caagaaata aacaccagc 
      gatgtagaa attttatca aaaaatgat ccacaacca 
      ccgctggtt aatcaagtg gacgaaaat ccgaatcag 
      ggtgggaac gacgacaga ccggatcaa ccggaaaac 
      gaaagagaa aaatcagtc cctaataac accaccaaa 
      ttagatact tctgacgat gcggttgaa gtttttgaa 
      tcgccagat gtagttttg cgcaaaggg cctaacgga 
      aaatatatg tttgattat ggcgacaat tacgaccca 
      atgacctcg cgctacgcc cctcaggaa gacagtcag 
      agaggtcgc gccgggccc ccttacgtc atcacggaa 
      gcccgcctt aaagaaatc cgggaacac ttcatgtac 
      ccttactat gaccgcggt ggcaatgct gataacgaa 
      ggagattat caaaaagcc atccagaca tcaaccccc 
      caagtacac aaaaatctc aacttccag ttgcccttc 
      ttcggcttt agatacaac tacactcga gtctctcta 
      aacggttat ctggaattc agtgacccg ccccaaaat 
      tacgaaacc tatcctttg gtattcccg gtgaaagac 
      tggccgaaa aagaacgat ccggctttc attgggatt 
      ttttacagc aaatgccgg attggcaat ttgcgagtt 
      gaggatatc gaccagaga atgcccggc gtttacttc 
      cggcttgaa cgggatttg agggaacgg aacgaccag 
      atgggcgtt gagattagg gaacgcttg aagtgggat 
      atccgagag ggtgttatt ggggctgat aacttcaac 
      cctaaacac gccattatt gccacttgg aagaacgtt 
      tcttttgct ggaggattc gccaacgcg ctgtacaaa 
      accaacacc ttccagctg gttttggcc actgatgaa 
      gtctacacc tatgccatg tttaattac ttgaacctt 
      gactggacc agtcatact gaagccggt ggtgacacc 
      atcaatggt gagggcggt attcccgca tatgtgggc 
      tttaacgca ggaaacggt actcgatcg taccaatac 
      gtgccatac agtcaaaat tcggtcatt cgagacctg 
      actgggacc ggtttcggt aatgaaaaa aaagggcgt 
      cactatttc cgaatcgac gaaaacatt tacctcgga 
      agttgcaat aaggacatc gacggtgct aacttagac 
      ctgtttttc gcaccagaa agcgggaac atgctcggt 
      ggcaccatt gttaacatc acaggcccg tgttttaaa 
      ccaaccgac cagattatt tgcaaattt gacactgct 
      gacgaagtt tacggcgtt gtggtcagc agcaatcgg 
      gcgatttgc atccaaccg ccacttttg gtcgaagga 
      tatgtcaag cttgagatc gcgattggg cccggaaca 
      tacaaatgg aaggggaaa tattatgtt gaaaccccg 
      gcaactgcc actcaaaaa atcttcttc gacgacatg 
      agctacaac gagaaatcg ccggccgag attgggttc 
      acttgggaa aagcagaat ttaacaacg aacgaaaac 
      gccaacatc cgaatttcg ctttggggg taccgggaa 
      acgagcgtc cgtcctgac ttcctctac attaccgat 
      ttggccgag aatgtgcaa aacacgggg tcgtacaag 
      attatcccg tcgacgtac aagaaccgc aacaatgag 
      aaattgatt gatatacaa tttggtttc attcaaatc 
      aacttgact gagtccatt ccggtgacc aacaagaac 
      ggacaaggc gaggtcaag ataacccct ctgatatgg 
      agccgcccg atacctttg ggctggtac ttcgggccg 
      cagtgggag cgcaaatac ggcacaaac tggcccaag 
      tatctctgc gataattgg ctcaaagac gaccgatat 
      ttgaagaat tttgccagc gaactgccg cagtgtccg 
      tgcactttg caacaggcc ttagcggac aaggggcgc 
      tttatgcca gattttgac tgcgacaaa gacgccaat 
      cccaagtgc tacttccac cgacaagcc gtgcactgc 
      gtcaaaacg ggctctccc tcgttgcaa ggggcggaa 
      cagcaatgc tgctacgac aaaaaccac tacctgatg 
      ttatcctat gaccagaaa tggggctcc tacccgcgc 
      cgtagccac aaccttggc tacctccct tggtacgaa 
      tcgaccaaa gtcccgact ttatcccaa tggtacaac 
      gacatgatc cctcgcttc gtctgctgc ttgtggcaa 
      gaggaaacc gccgttggt tgtgaaacc ctccgattt 
      gaaagacgt ccaacccaa gattgcgta gcttaccaa 
      gccccggct gttgccggt gtttacggc gacccccac 
      atcctcaca ttcgacgat ctcccttac accttcaac 
      ggaaaaggc gaatttgtt ttggtcaag tcggaaagt 
      gtgcgaaat cgcttggaa atccaagcc aggttcgag 
      caaatgccc atgaatgcc tatggcgaa gtaagggcc 
      acgcagttg acttcggtg gtggctaga ggcaatagt 
      accacgatt attgagatc aggagacgg ccacaagag 
      gctaggtgg aggtatcgg cttgatgtg atcgccgat 
      aggaggagg atttacttc gataggcct tcactcaaa 
      ttccaacac ttccccgga gttacagtt tacaccccg 
      acttatatt ttcaaccag tcggaagtt atcgtgatg 
      tttgattca ggggcgggt cttgaagtt gtcgaaaat 
      aacgggtat ttgacggcg cgggtttac ctcccgtgg 
      tcatttatc aaccaaacg cgcggtctt ttcggcaat 
      tggagcttc gatatcatg gacgatttt acgctccct 
      aacggtgac aagaaaggg gttgtgact aatttgaac 
      gacatgtcg agcgtttac accgacttt ggtatgaaa 
      tggatgttg gatgatgtg ggagatgac cagaaaggc 
      gccgctctt ttcttccga gagcacgga aaaactgcc 
      tcatcttac aacaacaaa acgttcgtc cccgagttc 
      agaatgtcc atagaagaa ttaatcccc gaaaatcgc 
      tccgagtac cgtagcagg gcttacgat ttatgcact 
      gcaacaaac tacgagtgc ttgtacgac tatgccatg 
      acttacaac agggatgtt gcccacttt tcggccaac 
      tacaaagct tccattaag gaacttaaa gagacgaat 
      cgcgacaat attgtgtct tgtggcgtc ctggaaacg 
      cctcgtttt ggacgcaag gacacgttc ctgtttgtg 
      ccaggcacc aaagtcact tttgagtgc agccaggac 
      tttattttg gtcggggat cagagacgg gagtgtttg 
      tcaaccggc gagtggaac ataccggaa tatggctac 
      accgagtgt ttacgccat caggagtat tcgtcgaga 
      aaggcggcc ataaccggg ggcatagta ctagccgtc 
      attatacca atattacta ataatcgcg ttttgcgcg 
      ttcagtttg ttcaaacga cttgcgaac gacaaaagt 
      tcgtggcaa tacaatgct gttaaaagc tcacgaccg 
      ttgagtccg atgtcggat gatgggtct tcgctccgt 
      agtcccgtt ccgagtgac cgctcgagt actagcagt 
      ttaggcaaa aagaggcga agttatgat aaggtttac 
      cgtactcat gagcccttg gaagggttg ccggagaca 
      gagtttgag gaaaagccg tgggacccc aacgaattt 
      gaacccgac agtaaacca acaagcccg acttcggtc 
      atttacacc cagcccttt agtaaacct gacttggtc 
      aagtttcag tcaaatccg aacttggat cggttcact 
      tacaactcc gatgattac acactgccg gtgaaaaag 
      aaccgaatg agcagacaa caagagtat tcgcaacgc 
      caggctggt atcgcctcc ggtatcgtc ctagctgtt 
      attatccca ctcgtcctt ttattcgtt tatatcgca 
      tatagattt ttcgagaaa cgacaacag gaaaaggac 
      gaagaggaa caagatgcg ttgaattac gaggccaag 
      aaaagggaa gcccaagaa attgccgct agaaaatta 
      accgcaggg gagcatttc gacgatgat acggccagt 
      agcatcgtc agcgggggc aaagaaacc acaatcgat 
      tga
    mRNA sequence
    • >TC008407 RA
      agagaaata ttttttagt cactcctat cactgttat 
      ctattgttt cgaaacatt ttgtcttat taagtgact 
      aatcggtcc gataatcca ttgcaaaat tgtctaaat 
      taatcgatg aattacaca aatgcctca tcgccattg 
      cctgtatct ttcttatca gtcataatg ataaaaacg 
      aaacattag atgaatcag atagtccga gcacctgta 
      acagaaaag attcgaatt ttctctcgt acatgcttt 
      ttttctaaa actcgaaaa tcggcatct gccgtctac 
      gaaaatgtt gacaaagtg tgtgttggt gattcttct 
      actaagtgc atacaacca agctcaaga aataaacac 
      cagcgatgt agaaatttt atcaaaaaa tgatccaca 
      accaccgct ggttaatca agtggacga aaatccgaa 
      tcagggtgg gaacgacga cagaccgga tcaaccgga 
      aaacgaaag agaaaaatc agtccctaa taacaccac 
      caaattaga tacttctga cgatgcggt tgaagtttt 
      tgaatcgcc agatgtagt tttgcgcaa agggcctaa 
      cggaaaata tatgtttga ttatggcga caattacga 
      cccaatgac ctcgcgcta cgcccctca ggaagacag 
      tcagagagg tcgcgccgg gccccctta cgtcatcac 
      ggaagcccg ccttaaaga aatccggga acacttcat 
      gtaccctta ctatgaccg cggtggcaa tgctgataa 
      cgaaggaga ttatcaaaa agccatcca gacatcaac 
      cccccaagt acacaaaaa tctcaactt ccagttgcc 
      cttcttcgg ctttagata caactacac tcgagtctc 
      tctaaacgg ttatctgga attcagtga cccgcccca 
      aaattacga aacctatcc tttggtatt cccggtgaa 
      agactggcc gaaaaagaa cgatccggc tttcattgg 
      gatttttta cagcaaatg ccggattgg caatttgcg 
      agttgagga tatcgacca gagaatgcc cggcgttta 
      cttccggct tgaacggga tttgaggga acggaacga 
      ccagatggg cgttgagat tagggaacg cttgaagtg 
      ggatatccg agagggtgt tattggggc tgataactt 
      caaccctaa acacgccat tattgccac ttggaagaa 
      cgtttcttt tgctggagg attcgccaa cgcgctgta 
      caaaaccaa caccttcca gctggtttt ggccactga 
      tgaagtcta cacctatgc catgtttaa ttacttgaa 
      ccttgactg gaccagtca tactgaagc cggtggtga 
      caccatcaa tggtgaggg cggtattcc cgcatatgt 
      gggctttaa cgcaggaaa cggtactcg atcgtacca 
      atacgtgcc atacagtca aaattcggt cattcgaga 
      cctgactgg gaccggttt cggtaatga aaaaaaagg 
      gcgtcacta tttccgaat cgacgaaaa catttacct 
      cggaagttg caataagga catcgacgg tgctaactt 
      agacctgtt tttcgcacc agaaagcgg gaacatgct 
      cggtggcac cattgttaa catcacagg cccgtgttt 
      taaaccaac cgaccagat tatttgcaa atttgacac 
      tgctgacga agtttacgg cgttgtggt cagcagcaa 
      tcgggcgat ttgcatcca accgccact tttggtcga 
      aggatatgt caagcttga gatcgcgat tgggcccgg 
      aacatacaa atggaaggg gaaatatta tgttgaaac 
      cccggcaac tgccactca aaaaatctt cttcgacga 
      catgagcta caacgagaa atcgccggc cgagattgg 
      gttcacttg ggaaaagca gaatttaac aacgaacga 
      aaacgccaa catccgaat ttcgctttg ggggtaccg 
      ggaaacgag cgtccgtcc tgacttcct ctacattac 
      cgatttggc cgagaatgt gcaaaacac ggggtcgta 
      caagattat cccgtcgac gtacaagaa ccgcaacaa 
      tgagaaatt gattgatat acaatttgg tttcattca 
      aatcaactt gactgagtc cattccggt gaccaacaa 
      gaacggaca aggcgaggt caagataac ccctctgat 
      atggagccg cccgatacc tttgggctg gtacttcgg 
      gccgcagtg ggagcgcaa atacggcac aaactggcc 
      caagtatct ctgcgataa ttggctcaa agacgaccg 
      atatttgaa gaattttgc cagcgaact gccgcagtg 
      tccgtgcac tttgcaaca ggccttagc ggacaaggg 
      gcgctttat gccagattt tgactgcga caaagacgc 
      caatcccaa gtgctactt ccaccgaca agccgtgca 
      ctgcgtcaa aacgggctc tccctcgtt gcaaggggc 
      ggaacagca atgctgcta cgacaaaaa ccactacct 
      gatgttatc ctatgacca gaaatgggg ctcctaccc 
      gcgccgtag ccacaacct tggctacct cccttggta 
      cgaatcgac caaagtccc gactttatc ccaatggta 
      caacgacat gatccctcg cttcgtctg ctgcttgtg 
      gcaagagga aaccgccgt tggttgtga aaccctccg 
      atttgaaag acgtccaac ccaagattg cgtagctta 
      ccaagcccc ggctgttgc cggtgttta cggcgaccc 
      ccacatcct cacattcga cgatctccc ttacacctt 
      caacggaaa aggcgaatt tgttttggt caagtcgga 
      aagtgtgcg aaatcgctt ggaaatcca agccaggtt 
      cgagcaaat gcccatgaa tgcctatgg cgaagtaag 
      ggccacgca gttgacttc ggtggtggc tagaggcaa 
      tagtaccac gattattga gatcaggag acggccaca 
      agaggctag gtggaggta tcggcttga tgtgatcgc 
      cgataggag gaggattta cttcgatag gccttcact 
      caaattcca acacttccc cggagttac agtttacac 
      cccgactta tattttcaa ccagtcgga agttatcgt 
      gatgtttga ttcaggggc gggtcttga agttgtcga 
      aaataacgg gtatttgac ggcgcgggt ttacctccc 
      gtggtcatt tatcaacca aacgcgcgg tcttttcgg 
      caattggag cttcgatat catggacga ttttacgct 
      ccctaacgg tgacaagaa aggggttgt gactaattt 
      gaacgacat gtcgagcgt ttacaccga ctttggtat 
      gaaatggat gttggatga tgtgggaga tgaccagaa 
      aggcgccgc tcttttctt ccgagagca cggaaaaac 
      tgcctcatc ttacaacaa caaaacgtt cgtccccga 
      gttcagaat gtccataga agaattaat ccccgaaaa 
      tcgctccga gtaccgtag cagggctta cgatttatg 
      cactgcaac aaactacga gtgcttgta cgactatgc 
      catgactta caacaggga tgttgccca cttttcggc 
      caactacaa agcttccat taaggaact taaagagac 
      gaatcgcga caatattgt gtcttgtgg cgtcctgga 
      aacgcctcg ttttggacg caaggacac gttcctgtt 
      tgtgccagg caccaaagt cacttttga gtgcagcca 
      ggactttat tttggtcgg ggatcagag acgggagtg 
      tttgtcaac cggcgagtg gaacatacc ggaatatgg 
      ctacaccga gtgtttacg ccatcagga gtattcgtc 
      gagaaaggc ggccataac cgggggcat agtactagc 
      cgtcattat accaatatt actaataat cgcgttttg 
      cgcgttcag tttgttcaa acgacttgc gaacgacaa 
      aagttcgtg gcaatacaa tgctgttaa aagctcacg 
      accgttgag tccgatgtc ggatgatgg gtcttcgct 
      ccgtagtcc cgttccgag tgaccgctc gagtactag 
      cagtttagg caaaaagag gcgaagtta tgataaggt 
      ttaccgtac tcatgagcc cttggaagg gttgccgga 
      gacagagtt tgaggaaaa gccgtggga ccccaacga 
      atttgaacc cgacagtaa accaacaag cccgacttc 
      ggtcattta cacccagcc ctttagtaa acctgactt 
      ggtcaagtt tcagtcaaa tccgaactt ggatcggtt 
      cacttacaa ctccgatga ttacacact gccggtgaa 
      aaagaaccg aatgagcag acaacaaga gtattcgca 
      acgccaggc tggtatcgc ctccggtat cgtcctagc 
      tgttattat cccactcgt ccttttatt cgtttatat 
      cgcatatag atttttcga gaaacgaca acaggaaaa 
      ggacgaaga ggaacaaga tgcgttgaa ttacgaggc 
      caagaaaag ggaagccca agaaattgc cgctagaaa 
      attaaccgc aggggagca tttcgacga tgatacggc 
      cagtagcat cgtcagcgg gggcaaaga aaccacaat 
      cgattgatt tagtaatga catgatcct agtgtttgt 
      tatttttta gaatagcta ttaagagta tgagttagg 
      tttttagat tttaggcaa gtatctagc actgtctgt 
      tgtacaaaa tattattct ctttttatt tgtgttacg 
      atttttaat aaacattta gaataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY