Status
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Gene information
General information
TC008406 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9851968..9857326
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008406
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01346

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 5

    wings blistered - Number of animals showing the phenotype: 2

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008406 RA
      MAEPDLNVD NLIQRLLEV RGCRPGKSV QMSEAEVRG 
      LCLKSREIF LQQPILLEL EAPLKICGD IHGQYTDLL 
      RLFEYGGFP PEANYLFLG DYVDRGKQS LETICLLLA 
      YKIKYPENF FLLRGNHEC ASINRIYGF YDECKRRYN 
      IKLWKTFTD CFNCLPIAA IIDEKIFCC HGGLSPDLQ 
      GMEQIRRIM RPTDVPDTG LLCDLLWSD PDKDVQGWG 
      ENDRGVSFT FGADVVSKF LNRHDLDLI CRAHQVVED 
      GYEFFAKRQ LVTLFSAPN YCGEFDNAG GMMSVDETL 
      MCSFQILKP SEKKAKYQY QGVNSGRPS TPQRNPTTK 
      KK
    • >TC008406 RB
      MSEAEVRGL CLKSREIFL QQPILLELE APLKICGDI 
      HGQYTDLLR LFEYGGFPP EANYLFLGD YVDRGKQSL 
      ETICLLLAY KIKYPENFF LLRGNHECA SINRIYGFY 
      DECKRRYNI KLWKTFTDC FNCLPIAAI IDEKIFCCH 
      GGLSPDLQG MEQIRRIMR PTDVPDTGL LCDLLWSDP 
      DKDVQGWGE NDRGVSFTF GADVVSKFL NRHDLDLIC 
      RAHQVVEDG YEFFAKRQL VTLFSAPNY CGEFDNAGG 
      MMSVDETLM CSFQILKPS EKKAKYQYQ GVNSGRPST 
      PQRNPTTKK K
    CDS sequence
    • >TC008406 RA
      atggcggag cctgatcta aacgtcgac aatttaatt 
      caaagatta ttagaagta cgaggatgt cgtcccgga 
      aagtctgta caaatgtcc gaggcggaa gtccgcgga 
      ctttgctta aaatcccga gaaattttc ctccaacag 
      cccatactt ttggaactg gaagctccc ttaaaaatc 
      tgcggggac attcatggc caatacaca gatctgctc 
      cgtcttttt gaatatggg ggctttcct cctgaagcc 
      aattacctg ttccttggc gactacgtt gatcgtggt 
      aaacaatcg ctagagacc atctgcctg cttctggcc 
      tataaaatt aaatatcct gaaaacttc ttcctcctc 
      agaggaaac cacgaatgc gcgtcgatt aaccgaata 
      tacggattt tacgacgaa tgcaagaga cggtacaac 
      atcaagttg tggaaaacc ttcaccgat tgcttcaac 
      tgcttgcca atagctgcg ataatcgac gagaaaatt 
      ttctgctgt cacgggggg ctcagtcct gacctgcaa 
      ggcatggag cagatccgt cgtattatg cgcccgacc 
      gatgtacct gacacaggt ctgctgtgc gatttgctg 
      tggtccgac cctgataaa gacgtgcaa ggttggggt 
      gagaacgac cgcggagtg tcatttact ttcggcgct 
      gatgtcgtt agtaaattt ttgaaccgt cacgatcta 
      gatttgatc tgtcgcgcc catcaagtg gtggaggat 
      gggtacgaa tttttcgca aaaagacaa ctagtcacg 
      cttttctca gccccgaat tattgcggc gagtttgac 
      aacgctgga gggatgatg agtgtagat gaaacgtta 
      atgtgttct tttcagatt ttgaagccg tcggaaaag 
      aaagccaag tatcaatac caaggagtg aactcagga 
      cgtccctca acgcctcag agaaaccca acaactaaa 
      aagaaataa
    • >TC008406 RB
      atgtccgag gcggaagtc cgcggactt tgcttaaaa 
      tcccgagaa attttcctc caacagccc atacttttg 
      gaactggaa gctccctta aaaatctgc ggggacatt 
      catggccaa tacacagat ctgctccgt ctttttgaa 
      tatgggggc tttcctcct gaagccaat tacctgttc 
      cttggcgac tacgttgat cgtggtaaa caatcgcta 
      gagaccatc tgcctgctt ctggcctat aaaattaaa 
      tatcctgaa aacttcttc ctcctcaga ggaaaccac 
      gaatgcgcg tcgattaac cgaatatac ggattttac 
      gacgaatgc aagagacgg tacaacatc aagttgtgg 
      aaaaccttc accgattgc ttcaactgc ttgccaata 
      gctgcgata atcgacgag aaaattttc tgctgtcac 
      ggggggctc agtcctgac ctgcaaggc atggagcag 
      atccgtcgt attatgcgc ccgaccgat gtacctgac 
      acaggtctg ctgtgcgat ttgctgtgg tccgaccct 
      gataaagac gtgcaaggt tggggtgag aacgaccgc 
      ggagtgtca tttactttc ggcgctgat gtcgttagt 
      aaatttttg aaccgtcac gatctagat ttgatctgt 
      cgcgcccat caagtggtg gaggatggg tacgaattt 
      ttcgcaaaa agacaacta gtcacgctt ttctcagcc 
      ccgaattat tgcggcgag tttgacaac gctggaggg 
      atgatgagt gtagatgaa acgttaatg tgttctttt 
      cagattttg aagccgtcg gaaaagaaa gccaagtat 
      caataccaa ggagtgaac tcaggacgt ccctcaacg 
      cctcagaga aacccaaca actaaaaag aaataa
    mRNA sequence
    • >TC008406 RA
      atagatttg cgcagtgtg caaataagg tattgaagt 
      tgaaatttt gcgagttgt ggaattaac gatttgaat 
      tttggcgaa aatttggtt ttgacacgt tctttgacg 
      tctgaaggg ttgaacgaa gtgcatcgt tgggttggt 
      aagattgtc gctaatttt catttatta gtggaaaat 
      aaaaatggc ggagcctga tctaaacgt cgacaattt 
      aattcaaag attattaga agtacgagg atgtcgtcc 
      cggaaagtc tgtacaaat gtccgaggc ggaagtccg 
      cggactttg cttaaaatc ccgagaaat tttcctcca 
      acagcccat acttttgga actggaagc tcccttaaa 
      aatctgcgg ggacattca tggccaata cacagatct 
      gctccgtct ttttgaata tgggggctt tcctcctga 
      agccaatta cctgttcct tggcgacta cgttgatcg 
      tggtaaaca atcgctaga gaccatctg cctgcttct 
      ggcctataa aattaaata tcctgaaaa cttcttcct 
      cctcagagg aaaccacga atgcgcgtc gattaaccg 
      aatatacgg attttacga cgaatgcaa gagacggta 
      caacatcaa gttgtggaa aaccttcac cgattgctt 
      caactgctt gccaatagc tgcgataat cgacgagaa 
      aattttctg ctgtcacgg ggggctcag tcctgacct 
      gcaaggcat ggagcagat ccgtcgtat tatgcgccc 
      gaccgatgt acctgacac aggtctgct gtgcgattt 
      gctgtggtc cgaccctga taaagacgt gcaaggttg 
      gggtgagaa cgaccgcgg agtgtcatt tactttcgg 
      cgctgatgt cgttagtaa atttttgaa ccgtcacga 
      tctagattt gatctgtcg cgcccatca agtggtgga 
      ggatgggta cgaattttt cgcaaaaag acaactagt 
      cacgctttt ctcagcccc gaattattg cggcgagtt 
      tgacaacgc tggagggat gatgagtgt agatgaaac 
      gttaatgtg ttcttttca gattttgaa gccgtcgga 
      aaagaaagc caagtatca ataccaagg agtgaactc 
      aggacgtcc ctcaacgcc tcagagaaa cccaacaac 
      taaaaagaa ataaagtcg agtaattga cacctcttt 
      tcattttta ttcaactaa tgcatttgt aacgtttat 
      tctctaata ctgctagac agtcagtcg cattgtgtt 
      gaagtatta ttattttgt atatttatt tagttttgt 
      tcatttttt ttttaatta aattgtgat tattttgac 
      tgattagga gttttcttt ttgactgac tggtcgtat 
      ttctattgt aaataaata tacgcaatt attaattta 
      tttaggaac gagaccagt gattggtgt attatttaa 
      agaaactat tgttaaatt atcttgctt gcatttcca 
      tgtgcatca taaagtggc ccagccaaa gactttgtg 
      attggtcat taaatgttg tagaagaat catttttct 
      ttggttcct aaatgctgc aatcgcatc ttgacattt 
      tgcaatcac ctctttgta tagaaattg gcataaacg 
      cgatttttt cactcgtac tatcgttta tgttttata 
      ttttatttc gttctattg acaaatcta tttcaccta 
      atgtacttt aaatttgac aaataaatc tatatatta 
      ctagcgacg cctttttat ctttcaaac ctttgtcct 
      ttttctctg attaacaca atttagaat aagtggaaa 
      tttttctga tcctctaaa tatgagtaa tgagttgct 
      ttttatgac agttttatg aagaatcaa taaaaactc 
      gaattaaat tagaaaaac tggaagcgg aagaggtca 
      taatttgca ggaggtcag caaacagta ataaagtgc 
      ccaaagact a
    • >TC008406 RB
      atagatttg cgcagtgtg caaataagg tattgaagt 
      tgaaatttt gcgagttgt ggaattaac gatttgaat 
      tttggcgaa aatttggtt ttgacacgt tctttgacg 
      tctgaaggg ttgtaagtg acaaaattc ctcgtagaa 
      gtcgtttgg gttatttaa attcgtgtt aatgcgttt 
      tgttggata aatttgttt gaactaagc gccatgcgt 
      gcgttttta ggaacgaag tgcatcgtt gggttggta 
      agattgtcg ctaattttc atttattag tggaaaata 
      aaaatggcg gagcctgat ctaaacgtc gacaattta 
      attcaaaga ttattagaa gtacgagga tgtcgtccc 
      ggaaagtct gtacaaatg tccgaggcg gaagtccgc 
      ggactttgc ttaaaatcc cgagaaatt ttcctccaa 
      cagcccata cttttggaa ctggaagct cccttaaaa 
      atctgcggg gacattcat ggccaatac acagatctg 
      ctccgtctt tttgaatat gggggcttt cctcctgaa 
      gccaattac ctgttcctt ggcgactac gttgatcgt 
      ggtaaacaa tcgctagag accatctgc ctgcttctg 
      gcctataaa attaaatat cctgaaaac ttcttcctc 
      ctcagagga aaccacgaa tgcgcgtcg attaaccga 
      atatacgga ttttacgac gaatgcaag agacggtac 
      aacatcaag ttgtggaaa accttcacc gattgcttc 
      aactgcttg ccaatagct gcgataatc gacgagaaa 
      attttctgc tgtcacggg gggctcagt cctgacctg 
      caaggcatg gagcagatc cgtcgtatt atgcgcccg 
      accgatgta cctgacaca ggtctgctg tgcgatttg 
      ctgtggtcc gaccctgat aaagacgtg caaggttgg 
      ggtgagaac gaccgcgga gtgtcattt actttcggc 
      gctgatgtc gttagtaaa tttttgaac cgtcacgat 
      ctagatttg atctgtcgc gcccatcaa gtggtggag 
      gatgggtac gaatttttc gcaaaaaga caactagtc 
      acgcttttc tcagccccg aattattgc ggcgagttt 
      gacaacgct ggagggatg atgagtgta gatgaaacg 
      ttaatgtgt tcttttcag attttgaag ccgtcggaa 
      aagaaagcc aagtatcaa taccaagga gtgaactca 
      ggacgtccc tcaacgcct cagagaaac ccaacaact 
      aaaaagaaa taaagtcga gtaattgac acctctttt 
      catttttat tcaactaat gcatttgta acgtttatt 
      ctctaatac tgctagaca gtcagtcgc attgtgttg 
      aagtattat tattttgta tatttattt agttttgtt 
      cattttttt tttaattaa attgtgatt attttgact 
      gattaggag ttttctttt tgactgact ggtcgtatt 
      tctattgta aataaatat acgcaatta ttaatttat 
      ttaggaacg agaccagtg attggtgta ttatttaaa 
      gaaactatt gttaaatta tcttgcttg catttccat 
      gtgcatcat aaagtggcc cagccaaag actttgtga 
      ttggtcatt aaatgttgt agaagaatc atttttctt 
      tggttccta aatgctgca atcgcatct tgacatttt 
      gcaatcacc tctttgtat agaaattgg cataaacgc 
      gattttttc actcgtact atcgtttat gttttatat 
      tttatttcg ttctattga caaatctat ttcacctaa 
      tgtacttta aatttgaca aataaatct atatattac 
      tagcgacgc ctttttatc tttcaaacc tttgtcctt 
      tttctctga ttaacacaa tttagaata agtggaaat 
      ttttctgat cctctaaat atgagtaat gagttgctt 
      tttatgaca gttttatga agaatcaat aaaaactcg 
      aattaaatt agaaaaact ggaagcgga agaggtcat 
      aatttgcag gaggtcagc aaacagtaa taaagtgcc 
      caaagacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY