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Gene information
General information
TC008390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9646243..9648450
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008390
    iBeetle screen iB_01343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008390 RA
      MDSDQINKY TFLTTYRKD YVKKPIKHL RAEAKNQEF 
      EDPISEGFK EFLILDKTD LKPPNENGD EYLEKFKQK 
      YKEIARIHF QEPLDIDAI RKNKVTKGA VTEYQMSYC 
      DIEKDTQFN LDKRNRKFT LADDAEIPL TTYGIYFRD 
      QTKALRPPL IIIPSNTLH THGNLVKKY KFVMMVTFL 
      DFRKPRLQS LHQRIHGPH WHIWRIYFR KKHPRTQTS 
      PRNLIVKIV SKFQFNKIF RSKFAMVLD QFQTTYRKD 
      YIWPYVRAY GLKTTPALP EAGDHLTCE CHCPKIDKT 
      KQVGLDQSE AAWSRLGPM GPLLDPKVY PVKVGASPE 
      SQVSRFNQP NVYLQKLKD KFPYIYECL RNAPPDDLI 
      SRINRDRLR TTYQVDYCK LQEYPDAPY DELLRAAGV 
      SGIAPCPAP VRLPGDPCR PNQRSIAYR PAVISNRVG 
      GGKSEGRSR GGDDNAMSS YCAGSCRGP LGSMTAGAT 
      EYQDAISRL GQMIMRDKI HFSLPKKII
    CDS sequence
    • >TC008390 RA
      atggattca gatcaaatt aataaatat acttttttg 
      acgacatac aggaaggat tatgtgaaa aagccgata 
      aaacatcta cgggcggag gcaaaaaat caagagttt 
      gaagacccc atcagtgag ggttttaag gagttttta 
      attttggat aaaacagat ctaaaacca ccgaacgaa 
      aacggcgac gaatacttg gaaaaattc aaacaaaaa 
      tacaaagag attgcaaga attcacttt caagaaccg 
      ttagatata gatgccata aggaaaaat aaagtcaca 
      aaaggggcc gttaccgaa taccagatg agctattgt 
      gacattgaa aaagatact caattcaac ttggataaa 
      cgcaacaga aaattcact ttggcagat gatgcggaa 
      ataccgtta acaacttat ggaatttat ttcagagac 
      cagacaaag gctttgaga cctccgctc attatcata 
      ccgtcaaat actttacac acgcatggt aatttggtg 
      aaaaaatac aaatttgtg atgatggta accttcctc 
      gactttagg aaacctcgc ctgcagtca ctacatcag 
      cgaatacac ggaccacat tggcacatt tggcgaatt 
      tattttaga aaaaagcat ccaaggacg caaacctcc 
      ccccgaaac ctgattgtc aaaattgtg tcaaaattt 
      cagttcaat aaaattttt cgttcaaaa ttcgcaatg 
      gttctcgat caatttcaa accacatac agaaaggac 
      tacatatgg ccttacgtg cgcgcttac ggactaaag 
      acaactcct gcgttacct gaagccggc gaccacctc 
      acgtgcgag tgccactgt ccaaaaatc gacaaaact 
      aaacaagtc gggctcgac caatccgaa gccgcatgg 
      agtcgtcta ggccccatg ggccccctt ctagacccc 
      aaagtgtac cctgtgaaa gtgggcgca agccccgaa 
      tcacaagtc agtcgcttc aaccaacct aacgtctac 
      ttgcaaaaa ctgaaagac aagttcccc tacatttac 
      gagtgtttg aggaatgca ccacctgat gatctgatc 
      tcgcgtatc aatcgcgat cggctaagg accacctac 
      caagtcgat tattgcaaa ttacaggag tatcctgac 
      gctccttac gacgagctg ttgcgagcg gctggagtg 
      tctggaatc gccccgtgt cctgcccct gtgcgactt 
      cctggcgat ccttgcagg cccaaccag aggtccatc 
      gcctaccgt cctgcggtt atcagtaac cgggttggg 
      ggcggtaag agcgaaggc cggtcaaga ggcggggac 
      gacaacgcc atgagctcc tactgcgct ggaagttgc 
      cgagggcct ctgggttca atgacagcc ggcgcgacg 
      gagtaccaa gacgccata tcccgtctc ggccagatg 
      ataatgcgt gacaaaatc cacttctct ctccccaaa 
      aaaataatc tag
    mRNA sequence
    • >TC008390 RA
      gttttgaaa taattttaa atgggtcta aaagttctt 
      taattaatc gtcaaaaaa tgggaaaaa ttcactatg 
      ttggcaaca acggacaag acgaaagta actagcaaa 
      tttcaaaat ttcgctgtc atacttgag aatgtgaaa 
      tggattcag atcaaatta ataaatata cttttttga 
      cgacataca ggaaggatt atgtgaaaa agccgataa 
      aacatctac gggcggagg caaaaaatc aagagtttg 
      aagacccca tcagtgagg gttttaagg agtttttaa 
      ttttggata aaacagatc taaaaccac cgaacgaaa 
      acggcgacg aatacttgg aaaaattca aacaaaaat 
      acaaagaga ttgcaagaa ttcactttc aagaaccgt 
      tagatatag atgccataa ggaaaaata aagtcacaa 
      aaggggccg ttaccgaat accagatga gctattgtg 
      acattgaaa aagatactc aattcaact tggataaac 
      gcaacagaa aattcactt tggcagatg atgcggaaa 
      taccgttaa caacttatg gaatttatt tcagagacc 
      agacaaagg ctttgagac ctccgctca ttatcatac 
      cgtcaaata ctttacaca cgcatggta atttggtga 
      aaaaataca aatttgtga tgatggtaa ccttcctcg 
      actttagga aacctcgcc tgcagtcac tacatcagc 
      gaatacacg gaccacatt ggcacattt ggcgaattt 
      attttagaa aaaagcatc caaggacgc aaacctccc 
      cccgaaacc tgattgtca aaattgtgt caaaatttc 
      agttcaata aaatttttc gttcaaaat tcgcaatgg 
      ttctcgatc aatttcaaa ccacataca gaaaggact 
      acatatggc cttacgtgc gcgcttacg gactaaaga 
      caactcctg cgttacctg aagccggcg accacctca 
      cgtgcgagt gccactgtc caaaaatcg acaaaacta 
      aacaagtcg ggctcgacc aatccgaag ccgcatgga 
      gtcgtctag gccccatgg gcccccttc tagacccca 
      aagtgtacc ctgtgaaag tgggcgcaa gccccgaat 
      cacaagtca gtcgcttca accaaccta acgtctact 
      tgcaaaaac tgaaagaca agttcccct acatttacg 
      agtgtttga ggaatgcac cacctgatg atctgatct 
      cgcgtatca atcgcgatc ggctaagga ccacctacc 
      aagtcgatt attgcaaat tacaggagt atcctgacg 
      ctccttacg acgagctgt tgcgagcgg ctggagtgt 
      ctggaatcg ccccgtgtc ctgcccctg tgcgacttc 
      ctggcgatc cttgcaggc ccaaccaga ggtccatcg 
      cctaccgtc ctgcggtta tcagtaacc gggttgggg 
      gcggtaaga gcgaaggcc ggtcaagag gcggggacg 
      acaacgcca tgagctcct actgcgctg gaagttgcc 
      gagggcctc tgggttcaa tgacagccg gcgcgacgg 
      agtaccaag acgccatat cccgtctcg gccagatga 
      taatgcgtg acaaaatcc acttctctc tccccaaaa 
      aaataatct agccgtcgt ccaaacaat ttttatttg 
      ccctgccac tcggaaaat ggatttcgt tacgatttt 
      gacaagatt gtcacaaaa tttcgtgtg ttatttatt 
      tgtaaagta cttgttcgg tctcctccg tcccaaatt 
      gtcgtcttt tagcatgct tgtgtatca gaaacaata 
      gtaaggcca agaaaccaa atttgcagt gatacgaaa 
      tgaccgtgg acaaggaaa ctttcttga ccaactacc 
      ggaaagact acaaatggc ccagtttcg tgccttcgg 
      tggccaagt aagtttgtg actattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY