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Gene information
General information
TC008372 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9234800..9243032
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008372
    iBeetle screen iB_01342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008372 RA
      MTAVERKGT FKVEYLQKI EREVQDRWK KEKLHEIDA 
      PKQPRKSED DKFLCTFPF PYMNGRLHL GHTFSLSKA 
      EFSVRYHKL KGKNALFPF GFHCTGMPI KACADKLKR 
      EMEMFGYPP KFPVEEEIK VEENDDVVI KDKSKGKKS 
      KAVAKAGAA KYQWQIMYS LGLQDEEIK KFADADYWL 
      DYFPPLAVQ DLDRFGVYV DWRRTFITT DVNPFFDSF 
      VRWQYIRLK ERNKIKFGK RYTIYSPRD GQPCMDHDR 
      STGEGVGPQ EYTLIKMKL LPPYPAKLS KLANKPVYL 
      VAATLRPET MYGQTNCWV RPDMKYAAV AVKSGEIFI 
      CTERSARNM SYQGFTEVD GKFEIVAHL VGQDLLGCA 
      LKAPLTQYD KIYALPMLT IKEDKGTGV VTSVPSDSP 
      DDYAALVDL KKKQPFREK YGIKDEMVL PFEPVPIVE 
      VPDFGKLSA VTVYEKLKI QSQNDREKL LEAKEMVYL 
      KGFYDGVMI VGEFKGKKI QDIKKSLQK VLIDKNEAV 
      IYYEPEKSI ISRSGDECV VALCDQWFL DYGEESWKK 
      LAHKVLDQM NTYHEEVRR NFIGCLDWL REHACSRTY 
      GLGSKLPWD EQWLIESLS DSTIYNAYY SVAHLLQGN 
      SFRGNKPNS LGIKPEQMT PEVWDYIFF KNAPFPAKS 
      GIKKESLDL MKHEFNYWY PVDVRVSGK DLVQNHLTY 
      FIYNHCAIW PEEEDKWPR GVRANGHLL LNSAKMSKS 
      DGNFLTLSE AVEKFSADG TRLCLADAG DSIEDANFV 
      ESMADAGIL RLYTFIEWV KEILENKGQ LRTGPATTF 
      NDEVFQSEI NLKIKETDE FYGKMLFKE ALRSGFFEL 
      QSVRDKYRE LCLEGMHAE LIVRFIEVQ AILLAPICP 
      HVSEQVWKL LGKKSSIFK ATWPQVGQI DEIKIKSSE 
      YLMETAHSF RVHLKTYLQ GIRTKANPN PAPVPKPDV 
      LNIWVAKTF PAWQSCILT TLKNHYEKS KEFPDNKVL 
      AMEFGSKPE LKKYMKRVM PFVQATREK VEQLGPKAL 
      ALTLEFNEA EVLTNNSVY LANTLNVDE VVVKFTDDE 
      AADEKMKEC CPGSPFVQF SSKPGVKVE FVNPVPNSG 
      FFSQVLVIS EGDTYAKIV QKLAREIKG IKSAQSIQL 
      WRFQDPVLG PRKIPTFQE PTKDKVLIK SDSIFKVNV 
      QEKKVSVVS GSNVSEVGQ SVIYLVV
    CDS sequence
    • >TC008372 RA
      atgactgca gttgagcga aagggcacg ttcaaggta 
      gaatatcta caaaaaatc gagcgtgaa gttcaggac 
      aggtggaag aaagagaaa ttgcacgaa atcgacgcg 
      cccaaacag ccccgaaaa tcagaagat gacaaattt 
      ttgtgcact tttccattt ccatacatg aacggccgt 
      ttacacctt ggccatacg ttctctcta tcaaaggct 
      gaattttcc gtcaggtat cacaaactg aaggggaaa 
      aatgcgttg tttcccttc ggttttcac tgcaccgga 
      atgccgatt aaagcatgt gctgataaa ttaaaacgc 
      gagatggaa atgttcggg tacccccct aaattccca 
      gtcgaggaa gaaataaaa gttgaggag aatgatgac 
      gttgttatt aaagataag agtaaagga aaaaagagt 
      aaagcagtg gctaaagcg ggcgctgcc aaataccag 
      tggcagatt atgtacagt ttagggctc caggacgag 
      gagataaaa aaatttgcg gatgctgat tactggctg 
      gattatttc ccgcccctt gccgtccag gacttggac 
      cgatttgga gtttacgtc gactggaga cgcaccttc 
      ataaccacc gacgtgaac ccctttttc gattccttc 
      gtccgttgg caatacatt cgtttgaaa gaacgcaac 
      aaaattaaa ttcggtaaa cgttacacg atttattca 
      cctcgcgat ggccagccg tgtatggac catgatagg 
      tccaccggc gaaggggtg ggtccccag gagtatact 
      ttaattaaa atgaagtta ttacctcca tatccggcg 
      aaattgagt aaactcgcc aataaaccg gtttatcta 
      gtagcggcg actttaagg cccgagaca atgtacgga 
      cagactaat tgttgggtc aggcctgat atgaaatat 
      gccgcagtt gcggtcaaa tctggcgaa atttttatt 
      tgcaccgag cgctcagct cggaatatg agctatcaa 
      gggtttacg gaggttgat gggaaattt gagattgtt 
      gcgcatttg gtgggacag gatcttctg ggttgcgcc 
      ctcaaagcg cccttgacc caatatgac aaaatttac 
      gccttaccc atgcttacc atcaaagag gataaaggt 
      acaggagtt gtgacaagt gtcccttct gattctccc 
      gacgattac gctgcgcta gtcgatttg aaaaagaag 
      cagcccttt agggagaaa tacgggatt aaagacgaa 
      atggttcta ccatttgag cccgtcccc atagttgaa 
      gtacccgat tttggtaaa ctttcggcc gtaaccgtg 
      tacgaaaaa ttgaaaatt cagagtcaa aatgaccga 
      gaaaagctg ctcgaggcc aaagaaatg gtctatttg 
      aagggtttt tatgacggg gtgatgatc gtgggcgaa 
      tttaagggc aaaaaaatc caggacatc aaaaaatcg 
      cttcagaag gtgctgatt gataaaaac gaggcggtt 
      atttattac gaacccgag aaaagtata atttcgaga 
      tcgggggac gagtgcgtg gttgctttg tgtgaccag 
      tggttcctt gattacggg gaagagtcc tggaagaaa 
      ttggcacat aaagtgcta gatcagatg aatacgtac 
      catgaggaa gtccggagg aacttcatt gggtgtctg 
      gattggctc cgtgagcac gcctgttcc agaacctac 
      ggacttggt tccaaatta ccatgggat gaacaatgg 
      ttaatcgaa tccttgtct gactctacc atctacaac 
      gcctactac tccgtcgct catttgctc caaggcaat 
      tcgttccgt ggtaataaa cccaactct cttggtatc 
      aaaccggag cagatgact cccgaagtt tgggattac 
      atcttcttc aaaaatgct ccattcccg gcgaaatcc 
      gggattaag aaagaatct ctcgatttg atgaaacac 
      gagtttaat tattggtat cctgttgat gtgagagtg 
      tctgggaag gatttggta cagaaccat ttgacgtat 
      tttatttac aatcattgt gcgatttgg cctgaagag 
      gaggataag tggccaaga ggtgtgcga gctaatggt 
      catctttta ctcaattcg gcgaaaatg tccaagtct 
      gacggtaat ttcttgact ttgagtgaa gctgtggag 
      aagtttagc gcagatgga acgagactg tgtctcgct 
      gatgccggg gattcgatc gaagatgcg aattttgtt 
      gaaagtatg gcagatgca gggattttg aggctgtac 
      acgtttatt gaatgggtg aaggagatc ttggaaaat 
      aaaggacaa ttgaggact ggccccgca acgacgttc 
      aacgacgaa gtattccag agcgaaatc aacctgaaa 
      atcaaagag acggacgaa ttctacggc aaaatgctg 
      tttaaagag gccctcagg agcggtttt ttcgaactt 
      caatccgtc agagacaaa tacagggaa ttgtgtttg 
      gagggaatg cacgcggag ttgatcgtt cgtttcatc 
      gaagttcag gcaatactc ctcgctccc atctgcccg 
      cacgtgtcc gaacaagtt tggaaatta ctgggaaaa 
      aaaagcagt atcttcaaa gcgacgtgg cctcaagtc 
      ggacaaatc gacgagatc aaaattaaa tcgtcggaa 
      tatctcatg gaaaccgct cattcgttc cgggtgcat 
      ctcaaaact tatcttcag ggaatcagg actaaggcc 
      aaccccaac ccggcgccc gttcctaag ccagacgtg 
      ctaaatatt tgggtggcc aagaccttc cccgcatgg 
      cagagctgc attcttacc acgctgaaa aaccactac 
      gagaaaagc aaggaattt ccggacaat aaagtcctg 
      gccatggaa ttcggctca aagcccgag ctgaagaaa 
      tacatgaag cgtgtaatg cctttcgtt caagcgact 
      cgcgaaaaa gtcgaacag ctcggtcct aaggcactg 
      gcgttgact ctcgagttc aacgaggcc gaagtactg 
      actaataac agcgtttat ttggcgaat actttaaac 
      gtcgacgaa gtcgtagtg aaatttacg gacgatgaa 
      gcagccgat gaaaaaatg aaggagtgt tgtccgggc 
      tcgcccttt gtgcagttc tcgagcaaa ccgggagtg 
      aaagtcgag tttgttaat cccgttccc aactccgga 
      ttcttctcg caagtgctg gtcatcagc gagggggac 
      acgtacgcg aaaatagtg cagaaattg gccagggaa 
      atcaaaggg attaaaagc gcgcagtct atccagctt 
      tggagattc caagacccg gttttgggg cccagaaaa 
      atcccaact ttccaagaa cccaccaag gataaggtg 
      ttgataaag tccgacagt attttcaaa gtcaacgtt 
      caagagaag aaagtctca gttgtttcg ggatcaaac 
      gtgtccgaa gtcggacag tcagttatt tatttggta 
      gtttag
    mRNA sequence
    • >TC008372 RA
      cctggcaaa aagtgaggt taactcgcc catgtgtta 
      tgtcaacgt gttcagtgg ttttttaat ttaaacaaa 
      aaaagaagc taaaaaatg actgcagtt gagcgaaag 
      ggcacgttc aaggtagaa tatctacaa aaaatcgag 
      cgtgaagtt caggacagg tggaagaaa gagaaattg 
      cacgaaatc gacgcgccc aaacagccc cgaaaatca 
      gaagatgac aaatttttg tgcactttt ccatttcca 
      tacatgaac ggccgttta caccttggc catacgttc 
      tctctatca aaggctgaa ttttccgtc aggtatcac 
      aaactgaag gggaaaaat gcgttgttt cccttcggt 
      tttcactgc accggaatg ccgattaaa gcatgtgct 
      gataaatta aaacgcgag atggaaatg ttcgggtac 
      ccccctaaa ttcccagtc gaggaagaa ataaaagtt 
      gaggagaat gatgacgtt gttattaaa gataagagt 
      aaaggaaaa aagagtaaa gcagtggct aaagcgggc 
      gctgccaaa taccagtgg cagattatg tacagttta 
      gggctccag gacgaggag ataaaaaaa tttgcggat 
      gctgattac tggctggat tatttcccg ccccttgcc 
      gtccaggac ttggaccga tttggagtt tacgtcgac 
      tggagacgc accttcata accaccgac gtgaacccc 
      tttttcgat tccttcgtc cgttggcaa tacattcgt 
      ttgaaagaa cgcaacaaa attaaattc ggtaaacgt 
      tacacgatt tattcacct cgcgatggc cagccgtgt 
      atggaccat gataggtcc accggcgaa ggggtgggt 
      ccccaggag tatacttta attaaaatg aagttatta 
      cctccatat ccggcgaaa ttgagtaaa ctcgccaat 
      aaaccggtt tatctagta gcggcgact ttaaggccc 
      gagacaatg tacggacag actaattgt tgggtcagg 
      cctgatatg aaatatgcc gcagttgcg gtcaaatct 
      ggcgaaatt tttatttgc accgagcgc tcagctcgg 
      aatatgagc tatcaaggg tttacggag gttgatggg 
      aaatttgag attgttgcg catttggtg ggacaggat 
      cttctgggt tgcgccctc aaagcgccc ttgacccaa 
      tatgacaaa atttacgcc ttacccatg cttaccatc 
      aaagaggat aaaggtaca ggagttgtg acaagtgtc 
      ccttctgat tctcccgac gattacgct gcgctagtc 
      gatttgaaa aagaagcag ccctttagg gagaaatac 
      gggattaaa gacgaaatg gttctacca tttgagccc 
      gtccccata gttgaagta cccgatttt ggtaaactt 
      tcggccgta accgtgtac gaaaaattg aaaattcag 
      agtcaaaat gaccgagaa aagctgctc gaggccaaa 
      gaaatggtc tatttgaag ggtttttat gacggggtg 
      atgatcgtg ggcgaattt aagggcaaa aaaatccag 
      gacatcaaa aaatcgctt cagaaggtg ctgattgat 
      aaaaacgag gcggttatt tattacgaa cccgagaaa 
      agtataatt tcgagatcg ggggacgag tgcgtggtt 
      gctttgtgt gaccagtgg ttccttgat tacggggaa 
      gagtcctgg aagaaattg gcacataaa gtgctagat 
      cagatgaat acgtaccat gaggaagtc cggaggaac 
      ttcattggg tgtctggat tggctccgt gagcacgcc 
      tgttccaga acctacgga cttggttcc aaattacca 
      tgggatgaa caatggtta atcgaatcc ttgtctgac 
      tctaccatc tacaacgcc tactactcc gtcgctcat 
      ttgctccaa ggcaattcg ttccgtggt aataaaccc 
      aactctctt ggtatcaaa ccggagcag atgactccc 
      gaagtttgg gattacatc ttcttcaaa aatgctcca 
      ttcccggcg aaatccggg attaagaaa gaatctctc 
      gatttgatg aaacacgag tttaattat tggtatcct 
      gttgatgtg agagtgtct gggaaggat ttggtacag 
      aaccatttg acgtatttt atttacaat cattgtgcg 
      atttggcct gaagaggag gataagtgg ccaagaggt 
      gtgcgagct aatggtcat cttttactc aattcggcg 
      aaaatgtcc aagtctgac ggtaatttc ttgactttg 
      agtgaagct gtggagaag tttagcgca gatggaacg 
      agactgtgt ctcgctgat gccggggat tcgatcgaa 
      gatgcgaat tttgttgaa agtatggca gatgcaggg 
      attttgagg ctgtacacg tttattgaa tgggtgaag 
      gagatcttg gaaaataaa ggacaattg aggactggc 
      cccgcaacg acgttcaac gacgaagta ttccagagc 
      gaaatcaac ctgaaaatc aaagagacg gacgaattc 
      tacggcaaa atgctgttt aaagaggcc ctcaggagc 
      ggttttttc gaacttcaa tccgtcaga gacaaatac 
      agggaattg tgtttggag ggaatgcac gcggagttg 
      atcgttcgt ttcatcgaa gttcaggca atactcctc 
      gctcccatc tgcccgcac gtgtccgaa caagtttgg 
      aaattactg ggaaaaaaa agcagtatc ttcaaagcg 
      acgtggcct caagtcgga caaatcgac gagatcaaa 
      attaaatcg tcggaatat ctcatggaa accgctcat 
      tcgttccgg gtgcatctc aaaacttat cttcaggga 
      atcaggact aaggccaac cccaacccg gcgcccgtt 
      cctaagcca gacgtgcta aatatttgg gtggccaag 
      accttcccc gcatggcag agctgcatt cttaccacg 
      ctgaaaaac cactacgag aaaagcaag gaatttccg 
      gacaataaa gtcctggcc atggaattc ggctcaaag 
      cccgagctg aagaaatac atgaagcgt gtaatgcct 
      ttcgttcaa gcgactcgc gaaaaagtc gaacagctc 
      ggtcctaag gcactggcg ttgactctc gagttcaac 
      gaggccgaa gtactgact aataacagc gtttatttg 
      gcgaatact ttaaacgtc gacgaagtc gtagtgaaa 
      tttacggac gatgaagca gccgatgaa aaaatgaag 
      gagtgttgt ccgggctcg ccctttgtg cagttctcg 
      agcaaaccg ggagtgaaa gtcgagttt gttaatccc 
      gttcccaac tccggattc ttctcgcaa gtgctggtc 
      atcagcgag ggggacacg tacgcgaaa atagtgcag 
      aaattggcc agggaaatc aaagggatt aaaagcgcg 
      cagtctatc cagctttgg agattccaa gacccggtt 
      ttggggccc agaaaaatc ccaactttc caagaaccc 
      accaaggat aaggtgttg ataaagtcc gacagtatt 
      ttcaaagtc aacgttcaa gagaagaaa gtctcagtt 
      gtttcggga tcaaacgtg tccgaagtc ggacagtca 
      gttatttat ttggtagtt tagctactt ttataattt 
      aacttattt ggaaataaa aacttggtt acgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY