Status
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Gene information
General information
TC032897
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008363
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9179391..9180820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032897
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032897 RA
      MYLSYTHAQ GTIPPHLNE NFQPKEEDP SKNDYCSMY 
      CGRIQHTGC DCKLGPGRQ EQLARKGAA GLRAGIVEY 
      HNHLRNLVA NGSDTRGKA GKAANMMAI SYNLELEYL 
      ARCFLRSRF IGHQDECRT MANGRKLGQ NSGGFDQID 
      DSDKIIYKL IERWYEEIM EMTPSIFRK FKIERRVGH 
      FTTMAWGDV NAIGCGRVI VRNPLTLKF LYPSFESIF 
      LCNYGSTLP GNNKQFGVN IIGRPVYLP GEPCTLCPK 
      DYPCNKGYK ALCGEVIAP PTAVEYDFA LVDYAPQSP 
      KVKPFKDPP KPKPKPKTK AKPKTKTKT KPKTKPKPK 
      TKAKTKTKT KKTKTKQKQ KPKPTPKKG KYAPGKPPY 
      NKPKIEEDD DKKSSPKKK SRKKSKKPD LDDPDNRSI 
      KEGTSLVMI CSMSLLIVY FVY
    CDS sequence
    • >TC032897 RA
      atgtatctg agttacaca catgcacaa ggaactatc 
      ccaccgcat ctcaatgag aatttccaa ccgaaagaa 
      gaggaccct tcgaaaaat gactattgt agcatgtat 
      tgtgggagg atacagcat acgggttgt gactgcaaa 
      ctgggacca ggacgacaa gaacaactg gcaagaaaa 
      ggagcagcg ggactacgt gctggaatc gtagaatat 
      cacaatcat ttaagaaat ttggtcgca aatggaagt 
      gatactcgg ggcaaagcc ggaaaagca gccaacatg 
      atggctatt agttacaac ttagagctg gaatactta 
      gcaagatgt tttctaaga agtagattt atcggacat 
      caagatgaa tgcaggaca atggctaac ggcagaaaa 
      ttaggtcaa aattctgga ggctttgat cagatagat 
      gactccgat aaaataatc tataaacta attgagcga 
      tggtatgaa gaaatcatg gaaatgacg ccatccatt 
      tttagaaag tttaaaatt gaaagaagg gtagggcat 
      tttacaaca atggcttgg ggggatgtt aatgcaata 
      ggatgtggt cgcgtaatc gttagaaat ccactaaca 
      cttaaattt ttataccct tcctttgaa agcattttc 
      ctctgtaac tacggaagt acgcttcct ggaaacaac 
      aaacaattc ggggttaat atcattggg cggccagta 
      tatcttcca ggtgaacca tgcacactg tgtcccaaa 
      gactacccg tgtaataaa ggatacaaa gctttgtgc 
      ggagaagtc attgcccct ccaactgcc gttgaatat 
      gactttgcg ctagtagac tatgcccca caaagccca 
      aaggttaaa cctttcaaa gacccacct aaacctaaa 
      cctaaacct aaaactaaa gccaaaccc aaaaccaaa 
      actaaaact aaacctaaa actaaacct aaacctaaa 
      actaaagct aaaactaaa actaaaact aaaaaaact 
      aaaactaaa cagaaacag aaacctaaa ccaacaccc 
      aaaaaagga aaatacgcg cccggaaaa cccccatac 
      aataaaccc aaaatcgag gaagacgat gacaaaaaa 
      tcctcaccc aaaaaaaag tccaggaaa aaatccaaa 
      aaaccggat ctagacgac ccagacaac aggagcatc 
      aaagaagga acatcctta gtaatgatt tgttctatg 
      tcactacta atcgtatat tttgtgtac taa
    mRNA sequence
    • >TC032897 RA
      tgtttgaac gacattttt ttgaatgaa aagaaatac 
      gttttatga ctgattaaa atcatattt tagtgatgt 
      tccaatcaa ttttgctca ttacgatgt atctgagtt 
      acacacatg cacaaggaa ctatcccac cgcatctca 
      atgagaatt tccaaccga aagaagagg acccttcga 
      aaaatgact attgtagca tgtattgtg ggaggatac 
      agcatacgg gttgtgact gcaaactgg gaccaggac 
      gacaagaac aactggcaa gaaaaggag cagcgggac 
      tacgtgctg gaatcgtag aatatcaca atcatttaa 
      gaaatttgg tcgcaaatg gaagtgata ctcggggca 
      aagccggaa aagcagcca acatgatgg ctattagtt 
      acaacttag agctggaat acttagcaa gatgttttc 
      taagaagta gatttatcg gacatcaag atgaatgca 
      ggacaatgg ctaacggca gaaaattag gtcaaaatt 
      ctggaggct ttgatcaga tagatgact ccgataaaa 
      taatctata aactaattg agcgatggt atgaagaaa 
      tcatggaaa tgacgccat ccattttta gaaagttta 
      aaattgaaa gaagggtag ggcatttta caacaatgg 
      cttgggggg atgttaatg caataggat gtggtcgcg 
      taatcgtta gaaatccac taacactta aatttttat 
      acccttcct ttgaaagca ttttcctct gtaactacg 
      gaagtacgc ttcctggaa acaacaaac aattcgggg 
      ttaatatca ttgggcggc cagtatatc ttccaggtg 
      aaccatgca cactgtgtc ccaaagact acccgtgta 
      ataaaggat acaaagctt tgtgcggag aagtcattg 
      cccctccaa ctgccgttg aatatgact ttgcgctag 
      tagactatg ccccacaaa gcccaaagg ttaaacctt 
      tcaaagacc cacctaaac ctaaaccta aacctaaaa 
      ctaaagcca aacccaaaa ccaaaacta aaactaaac 
      ctaaaacta aacctaaac ctaaaacta aagctaaaa 
      ctaaaacta aaactaaaa aaactaaaa ctaaacaga 
      aacagaaac ctaaaccaa cacccaaaa aaggaaaat 
      acgcgcccg gaaaacccc catacaata aacccaaaa 
      tcgaggaag acgatgaca aaaaatcct cacccaaaa 
      aaaagtcca ggaaaaaat ccaaaaaac cggatctag 
      acgacccag acaacagga gcatcaaag aaggaacat 
      ccttagtaa tgatttgtt ctatgtcac tactaatcg 
      tatattttg tgtactaaa ctttcaaaa ttaataaag 
      taatcgcaa tgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY