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Gene information
General information
TC008343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8789970..8791743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008343
    iBeetle screen iB_01338

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008343 RA
      MIVREAVVL LILAFAALA KPKSEEKKD RVFDRELSD 
      KEHFENEHH NPQYDHEAF LGEEAKTFD QLPPEESKR 
      RLGLIVDKI DNNKDGYIS REELKDWIR FTQKRYITE 
      DVDRQWKQH NPENEESIP WERYQKLVY GFLDSMDPS 
      EAEKDSEGF SYKQMLKRD RRRWQVADL NGDDALTKE 
      EFMHFLHPE ESDHMKDIV VQETMEDID KDSDGKISL 
      KEYIGDMFK GDENEEEPE WVNNEREQF NTYRDKDGD 
      GFMDNEEVK NWILPPDFD HAEAEARHL IFEADSDAD 
      EQLTKEEIL NKYDLFVGS QATDFGEAL ARHDEF
    CDS sequence
    • >TC008343 RA
      atgattgtg agggaggct gtagtgttg ctaattttg 
      gcgtttgcc gccttagcg aagccaaaa tcagaagag 
      aagaaggac agggttttt gaccgtgag ttgagcgat 
      aaggaacat tttgaaaac gagcatcac aaccctcag 
      tatgaccat gaggcgttt ttgggggag gaagcaaag 
      acttttgac cagttacca cccgaagag agcaaacga 
      cgtttggga ttgatcgtt gataaaatc gacaataac 
      aaggacgga tacatttcg cgggaagaa ctgaaagac 
      tggatccgt ttcacccaa aagcgctac attactgaa 
      gatgtggat cgccagtgg aagcaacac aatcccgaa 
      aacgaggaa tctatccct tgggagcgt taccagaaa 
      ctggtttat gggtttttg gatagcatg gacccttcg 
      gaagcggag aaagatagc gaagggttt tcctacaag 
      cagatgttg aagcgggac aggaggcgg tggcaagtg 
      gctgatctt aacggtgac gatgcgctc actaaagaa 
      gagttcatg catttcctg catccggaa gaatccgac 
      catatgaaa gatatcgtg gtccaggaa accatggaa 
      gatattgat aaagacagc gacgggaaa atatctctc 
      aaggaatat attggcgat atgttcaaa ggggacgaa 
      aacgaggaa gagcccgaa tgggtcaac aatgaaagg 
      gaacaattt aatacatat cgggacaag gatggcgac 
      ggttttatg gacaatgag gaagtgaaa aattggatt 
      ctcccgccg gattttgac cacgccgaa gccgaagcg 
      cgacatttg atctttgag gctgattcg gacgccgac 
      gaacagcta acaaaggag gaaattttg aacaaatat 
      gatttgttt gtaggctcg caagcgacc gattttggc 
      gaagcgcta gcccgacac gacgaattt taa
    mRNA sequence
    • >TC008343 RA
      cccagctgt tgaacatca taaaaatct ttaaacagc 
      atcccggag agaactctg atgagttgg aagttttaa 
      aaaagattt ctcatttca agtgttgct tccattgtg 
      tccaaatac ccaaaaatc aattacaaa cacaagaaa 
      ccgcgcgtc ttaaaaggg acttcctga cctaaattt 
      gcagaaatg attgtgagg gaggctgta gtgttgcta 
      attttggcg tttgccgcc ttagcgaag ccaaaatca 
      gaagagaag aaggacagg gtttttgac cgtgagttg 
      agcgataag gaacatttt gaaaacgag catcacaac 
      cctcagtat gaccatgag gcgtttttg ggggaggaa 
      gcaaagact tttgaccag ttaccaccc gaagagagc 
      aaacgacgt ttgggattg atcgttgat aaaatcgac 
      aataacaag gacggatac atttcgcgg gaagaactg 
      aaagactgg atccgtttc acccaaaag cgctacatt 
      actgaagat gtggatcgc cagtggaag caacacaat 
      cccgaaaac gaggaatct atcccttgg gagcgttac 
      cagaaactg gtttatggg tttttggat agcatggac 
      ccttcggaa gcggagaaa gatagcgaa gggttttcc 
      tacaagcag atgttgaag cgggacagg aggcggtgg 
      caagtggct gatcttaac ggtgacgat gcgctcact 
      aaagaagag ttcatgcat ttcctgcat ccggaagaa 
      tccgaccat atgaaagat atcgtggtc caggaaacc 
      atggaagat attgataaa gacagcgac gggaaaata 
      tctctcaag gaatatatt ggcgatatg ttcaaaggg 
      gacgaaaac gaggaagag cccgaatgg gtcaacaat 
      gaaagggaa caatttaat acatatcgg gacaaggat 
      ggcgacggt tttatggac aatgaggaa gtgaaaaat 
      tggattctc ccgccggat tttgaccac gccgaagcc 
      gaagcgcga catttgatc tttgaggct gattcggac 
      gccgacgaa cagctaaca aaggaggaa attttgaac 
      aaatatgat ttgtttgta ggctcgcaa gcgaccgat 
      tttggcgaa gcgctagcc cgacacgac gaattttaa 
      tgcattttt agggagggc ttcaagggc cagtcatat 
      cgggagtct tccaattta ccaaaaaat ctcaataaa 
      gatgctgct ttctcttat tttaactct ttccatgta 
      ttggttcct ttatctcta cggatgaag gggcgatta 
      agtgttctt aatttagtg tgtatgtta gtttgtaat 
      actaaaatt tattgttta tggagatta tgtgtaggt 
      gttgtattt atgtgccat tccgctggc caaagcatt 
      tactaattt aagacaatt tctttaaga atgaggtcg 
      ttagcaccg gtctgatac ttaacgcga tgaatcctt 
      ggtgctaag attattttt atattatac aaatttccc 
      tctatcaat aaattataa gttttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY