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Gene information
General information
TC008338 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8734290..8737723
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008338
    iBeetle screen iB_01337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008338 RA
      MKRNLKTVF KFLLLSGTT VVFTVLLFR IFKIRSTFE 
      SHERGLPLE PSEVRIISN EKIDWHDYD AIKRDSLRR 
      GTGEQGKPA FLTAAESDN YEKLYKVNG FNAALSDQI 
      AIDRAVPDI RHPGCKSKK YLKDLPTVS VVVPFHNEH 
      WTTLLRTAA SVVNRSPPH LLKEVILVD DCSTKEFSK 
      KPLDDYLAA NLTKVRAIH LPERSGLIR ARLAGARVA 
      TADVLIFLD SHTEANVNW LPPLLEPIA QDYKTCVCP 
      FIDVIQYET FEYRAQDEG ARGAFDWEF FYKRLPLLP 
      EDLEHPTEP FKSPVMAGG LFAISRKFF WELGGYDEG 
      LDIWGGEQY ELSFKIWQC GGLMVDAPC SRVGHIYRK 
      YAPFPNPGK GDFVGRNYR RVAEVWMDE YAEYLYKRR 
      PHYRDIDPG DLTKQKALR EKLHCKPFK WFMEKVAFD 
      LPLKYPPIE PGDFGVGEI RNLAAPELC VDSGHKDRD 
      QVIGLAECV KGTNKNGEQ NFALTWHKD LRVKGKTLC 
      LDVSDPNDK ADIVLYPCH GSQGNQYWR YDVEKQWFL 
      HGGNPRCLD CDPGQRRLY VTKCDDQSK TQKWRFENV 
      NLTMIADWE NVGVRF
    CDS sequence
    • >TC008338 RA
      atgaaacgt aatctgaag acggtgttc aagtttctc 
      ttgctttca ggaacgacc gttgttttc acggttttg 
      ctattcagg atttttaaa attcgatcg acgtttgaa 
      agccatgaa cgaggtttg cctttagaa ccaagcgag 
      gtgaggatc attagtaat gaaaagatt gattggcat 
      gattatgat gctataaaa agggattca ttgaggaga 
      ggtactggg gaacaaggc aaacctgca tttttaact 
      gcagccgaa tcagataat tatgaaaag ttatacaaa 
      gttaatgga tttaatgct gccttaagt gatcaaatc 
      gcaattgat agagctgtg cctgatata cgtcatcca 
      ggatgtaaa tcaaaaaaa tatctcaaa gatctgcca 
      actgttagc gtcgtagtc ccctttcat aatgagcac 
      tggacaact ctgctccga acagctgcc agtgtagta 
      aaccgatca cctccacat ttgctcaaa gaagttatc 
      ctagtggat gactgtagc actaaagaa tttagcaaa 
      aaacctctc gacgattac ctcgccgct aatttaaca 
      aaagttcgg gcaattcac ttgccggaa agaagcggt 
      ttgatacgt gcccgcctt gcaggagct agagtagca 
      acagccgat gttttaatt tttttggat tcacatact 
      gaagctaat gttaattgg ctaccgcct cttctagaa 
      ccaatcgcg caagactac aagacttgc gtttgccca 
      tttattgat gtaattcag tatgagaca ttcgaatat 
      cgggcgcaa gatgaaggg gcaaggggg gctttcgac 
      tgggagttt ttctacaaa cgtcttcca ttgttgcct 
      gaggatttg gagcatccg actgaacct ttcaagagt 
      ccagtcatg gctggaggt ttgtttgcc atcagtagg 
      aaatttttc tgggagttg gggggttat gacgaaggg 
      ttggatatt tgggggggt gaacagtac gaattgagt 
      tttaaaata tggcagtgt ggaggctta atggtcgat 
      gctccctgt tcaagggtt ggacatatc tataggaaa 
      tatgccccg tttccaaat ccgggcaaa ggcgatttt 
      gttgggagg aattataga agagttgct gaggtctgg 
      atggacgaa tacgccgaa tatttgtac aaacgtcgc 
      cctcactat cgagatata gatcccggg gatttgaca 
      aaacaaaaa gccttaaga gagaagtta cactgtaag 
      ccattcaag tggtttatg gagaaagtg gcctttgat 
      ctacctctg aagtatccc ccgattgaa cctggagat 
      tttggagtc ggagaaata cgaaactta gcagctcct 
      gagttatgc gttgactcg ggccataag gaccgtgac 
      caggttata ggcttagct gaatgcgtc aaagggacg 
      aacaaaaac ggcgaacaa aacttcgcc ttgacctgg 
      cacaaggat ttgcgagta aagggcaaa actttgtgt 
      ctggatgtt tcagatcct aacgataag gcggatatt 
      gtcttgtac ccctgtcac ggatctcaa gggaatcag 
      tattggaga tacgatgtt gagaaacag tggtttttg 
      catgggggt aatccaagg tgtttggat tgcgatccc 
      ggccaacgt cgcctctac gtcacgaag tgtgacgac 
      caatccaaa actcaaaaa tggaggttc gaaaatgtt 
      aatttgacc atgatcgcc gattgggag aatgtcggt 
      gttcgcttc tag
    mRNA sequence
    • >TC008338 RA
      caataattg caatttgca acaatcgaa gcatttgcc 
      gttgttttt gtttacttt cgaaggtga accggttga 
      cagtagccg tcagttcgt gcacgagtg aggttatgt 
      taccgttca gacctcacc tttacctgt ttcagtatc 
      actattctt aaaccgttt atctttctt atcagtgca 
      gaatccgca aaataatca cagaaacat gccaacgtc 
      gataaaatg aaacgtaat ctgaagacg gtgttcaag 
      tttctcttg ctttcagga acgaccgtt gttttcacg 
      gttttgcta ttcaggatt tttaaaatt cgatcgacg 
      tttgaaagc catgaacga ggtttgcct ttagaacca 
      agcgaggtg aggatcatt agtaatgaa aagattgat 
      tggcatgat tatgatgct ataaaaagg gattcattg 
      aggagaggt actggggaa caaggcaaa cctgcattt 
      ttaactgca gccgaatca gataattat gaaaagtta 
      tacaaagtt aatggattt aatgctgcc ttaagtgat 
      caaatcgca attgataga gctgtgcct gatatacgt 
      catccagga tgtaaatca aaaaaatat ctcaaagat 
      ctgccaact gttagcgtc gtagtcccc tttcataat 
      gagcactgg acaactctg ctccgaaca gctgccagt 
      gtagtaaac cgatcacct ccacatttg ctcaaagaa 
      gttatccta gtggatgac tgtagcact aaagaattt 
      agcaaaaaa cctctcgac gattacctc gccgctaat 
      ttaacaaaa gttcgggca attcacttg ccggaaaga 
      agcggtttg atacgtgcc cgccttgca ggagctaga 
      gtagcaaca gccgatgtt ttaattttt ttggattca 
      catactgaa gctaatgtt aattggcta ccgcctctt 
      ctagaacca atcgcgcaa gactacaag acttgcgtt 
      tgcccattt attgatgta attcagtat gagacattc 
      gaatatcgg gcgcaagat gaaggggca aggggggct 
      ttcgactgg gagtttttc tacaaacgt cttccattg 
      ttgcctgag gatttggag catccgact gaacctttc 
      aagagtcca gtcatggct ggaggtttg tttgccatc 
      agtaggaaa tttttctgg gagttgggg ggttatgac 
      gaagggttg gatatttgg gggggtgaa cagtacgaa 
      ttgagtttt aaaatatgg cagtgtgga ggcttaatg 
      gtcgatgct ccctgttca agggttgga catatctat 
      aggaaatat gccccgttt ccaaatccg ggcaaaggc 
      gattttgtt gggaggaat tatagaaga gttgctgag 
      gtctggatg gacgaatac gccgaatat ttgtacaaa 
      cgtcgccct cactatcga gatatagat cccggggat 
      ttgacaaaa caaaaagcc ttaagagag aagttacac 
      tgtaagcca ttcaagtgg tttatggag aaagtggcc 
      tttgatcta cctctgaag tatcccccg attgaacct 
      ggagatttt ggagtcgga gaaatacga aacttagca 
      gctcctgag ttatgcgtt gactcgggc cataaggac 
      cgtgaccag gttataggc ttagctgaa tgcgtcaaa 
      gggacgaac aaaaacggc gaacaaaac ttcgccttg 
      acctggcac aaggatttg cgagtaaag ggcaaaact 
      ttgtgtctg gatgtttca gatcctaac gataaggcg 
      gatattgtc ttgtacccc tgtcacgga tctcaaggg 
      aatcagtat tggagatac gatgttgag aaacagtgg 
      tttttgcat gggggtaat ccaaggtgt ttggattgc 
      gatcccggc caacgtcgc ctctacgtc acgaagtgt 
      gacgaccaa tccaaaact caaaaatgg aggttcgaa 
      aatgttaat ttgaccatg atcgccgat tgggagaat 
      gtcggtgtt cgcttctag cgacgtact taatgtaaa 
      gtattatca gtgtttacc tacaccttt taaaatgtg 
      ctaaaaagt gattatttt acgtaattt aatgtaggt 
      atacatgtc cttacggtg catttcgaa ttcgaggac 
      tctaattgt ttatcaatg tttttccct gacgctgct 
      gtgatattt agcgtttat ttttagaat aaaattgtt 
      gccatattt tgaagttgt aaatggatt atttaaatt 
      tgatacctt ttataggcg gaattcttt gtaggatta 
      ttgtcaaat ccgatacgt atttaataa aaatatcca 
      caatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY