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Gene information
General information
TC008329 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 8550975..8571156
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008329
    iBeetle screen iB_01336
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008329 RA
      MKSPPAVTD DCDDIFGPP RTYPTGYCP PKNRPSMIS 
      AKYRQKEER RKVLKISLN KLKKIEDPE SSLCRSVLI 
      NNTMKRLQK EARDEKIQK QQLNYPRCY DNDNILNIK 
      NEFIKNESK TSGTFDEPQ VDFVVENDF DAKLAEEIV 
      TAKNMEVFN ELVEENLNN LEQSLSSNL VQEPMESNE 
      RFASRKRSF DDMEDCDVQ DVLKQFYMP PTPRMLTCI 
      DDDEDVNVV DAAPSPKKA KVDENFEPK SDFSETSMA 
      DVVCDKTGD FSVNNFINN NIPSERLRY TSQNETDSN 
      SYSCGHASM FNELQNNVY HNLIASLET
    CDS sequence
    • >TC008329 RA
      atgaaatct cctccagct gtgaccgac gactgcgat 
      gacatattc ggccctcca cgcacctac cccaccggt 
      tactgtccc cccaagaat cggccatcg atgatatca 
      gcgaaatac cggcaaaag gaagagcgt cggaaagtg 
      ttgaagatt agtttgaac aagctgaag aagatcgaa 
      gaccccgaa tcgagtctc tgccgatcg gttttgatc 
      aacaacacc atgaagcgg ctgcagaag gaggctcgc 
      gacgagaag atccagaag cagcagctc aactacccc 
      cgttgctac gacaacgac aacatcctc aacatcaag 
      aacgaattt attaagaac gagagcaag accagtggg 
      acttttgac gagccccag gtcgatttt gtggtggag 
      aacgacttt gacgcgaaa ctggccgaa gaaatcgtc 
      actgcgaag aacatggaa gtgttcaat gagctagtt 
      gaggagaac ttgaacaat ctcgagcag agtttgagc 
      tcgaacctg gtgcaggag ccgatggag agcaacgag 
      cggttcgcg tcgcgaaag cgctcgttc gacgatatg 
      gaagattgc gacgttcag gacgtgctg aagcagttt 
      tacatgccg ccgacccct cgcatgctg acttgcatc 
      gacgacgac gaggacgtg aacgtggtg gacgcggcg 
      ccttccccg aaaaaagcc aaagtagac gagaatttc 
      gagcctaaa agtgacttc agtgagacg agtatggcc 
      gacgtggtg tgtgataaa accggtgac tttagtgta 
      aataatttc attaataat aatataccg agcgagcgg 
      ctgaggtat acaagtcag aacgagacg gactcgaat 
      tcttacagt tgcggacat gcatccatg ttcaacgaa 
      ctgcagaat aacgtttat cacaattta attgcatca 
      ttggagacg tag
    mRNA sequence
    • >TC008329 RA
      gcgcaaggc agtactatc caagttctt gacggcagg 
      acatcgtac tatagtaca cgtagagtg cgcccaact 
      tttatgtgt cacctcttt ggaaatttg tgaacacct 
      tgacatttt taatgtagt ggataaaag gggattttt 
      tttcatttt ttatttttt gcgtgcggt ggcagtgac 
      caatgcaaa agtttatga tgatattcg atagtatta 
      attagtggt ttgactgaa ctatcgcga cccttaacc 
      tggattatc tttacatcg actcgggaa cccgcaatc 
      aagtccctg agacagagc ctgtggcta ccacaatga 
      aatctcctc cagctgtga ccgacgact gcgatgaca 
      tattcggcc ctccacgca cctacccca ccggttact 
      gtcccccca agaatcggc catcgatga tatcagcga 
      aataccggc aaaaggaag agcgtcgga aagtgttga 
      agattagtt tgaacaagc tgaagaaga tcgaagacc 
      ccgaatcga gtctctgcc gatcggttt tgatcaaca 
      acaccatga agcggctgc agaaggagg ctcgcgacg 
      agaagatcc agaagcagc agctcaact acccccgtt 
      gctacgaca acgacaaca tcctcaaca tcaagaacg 
      aatttatta agaacgaga gcaagacca gtgggactt 
      ttgacgagc cccaggtcg attttgtgg tggagaacg 
      actttgacg cgaaactgg ccgaagaaa tcgtcactg 
      cgaagaaca tggaagtgt tcaatgagc tagttgagg 
      agaacttga acaatctcg agcagagtt tgagctcga 
      acctggtgc aggagccga tggagagca acgagcggt 
      tcgcgtcgc gaaagcgct cgttcgacg atatggaag 
      attgcgacg ttcaggacg tgctgaagc agttttaca 
      tgccgccga cccctcgca tgctgactt gcatcgacg 
      acgacgagg acgtgaacg tggtggacg cggcgcctt 
      ccccgaaaa aagccaaag tagacgaga atttcgagc 
      ctaaaagtg acttcagtg agacgagta tggccgacg 
      tggtgtgtg ataaaaccg gtgacttta gtgtaaata 
      atttcatta ataataata taccgagcg agcggctga 
      ggtatacaa gtcagaacg agacggact cgaattctt 
      acagttgcg gacatgcat ccatgttca acgaactgc 
      agaataacg tttatcaca atttaattg catcattgg 
      agacgtagc agtttagta acagtattt aggcaacga 
      tttttttgg ataacgtct ttatttatg tgatagtgt 
      gaatgattt acttaattg tttcatccg cccgtgaaa 
      actttcatt ctccctggt gctaagcgg cgtcactgt 
      ccatcggaa attttcacg gggcctaaa ttacgtaac 
      tttcacatt gaatttttt tcaacgttc aattttgta 
      atgctttta gctctctac ggctactaa agaattatg 
      ttcctaacg tcttctatt tccaacgtt ttattgcgt 
      tactgattt ctttgttct agtgagtag agagcgaaa 
      atatctgtg atattacaa ttaaagcaa aatgtgtta 
      aaggttaga tcaatttta aaaagcctg tacaatata 
      gacttagat aatacaatg agagttatc tgtaaagat 
      gcttgtaga ttgaaaaag tttgttggt gttatttac 
      cacaaactc ctttacaat ttcctggca cttgtagaa 
      gcatttaaa gaaactttg tgtctttgt ggctttaat 
      agcgatatt gtccccgtt tcttcatat ccatatcag 
      atgatctat gctgcagaa gtttagata catattttt 
      gaaaataat gaaaagtgg cattaacga caatttttg 
      ataacattt ttgaatttt ttatacctg ccaaaatga 
      gacgtattt ttgacgtag cacagtgct ttaactctc 
      ccatctcat cccggaatc ggttatcat gttttaaat 
      tctattttt ttacgtttt tcttgaata tttttgtat 
      attttacat gcaaattat cgttgaaaa aattattgt 
      aactttttt atgcaattt agttagtgt ttgtgttat 
      gggttttgc catattttg tttttatat gtaacaatt 
      tatgtgtac atatgtagt tatcaatta ctatcctct 
      gttagttta tttatgtat tatatattt tcctcgtta 
      tgtaattaa ttactcaaa taacattat ttatttgga 
      aaaccatta tcacacaac ttgttttat tgttggaac 
      tgattgaac aaaagaaac ctcgttaca ataattttt 
      ggagaaaaa taagtttct attgaatac caaaacagt 
      gttaccgca gatttacca ctcttgtat aatattatt 
      actattatc atatgaatt attattgta ttgtatgta 
      cattaggca ttatcaaaa tttcaggta atgcaataa 
      agtacctga aaaata