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Gene information
General information
TC008308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 8205694..8305606
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008308
    iBeetle screen iB_01335
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008308 RA
      MHATTCFVL LVTSFLYVG CDNWQEWWT YDGISGPGY 
      WGLMNPQWN MCSKGRRQS PINVEPEKL LFDPHLQRI 
      HLDKHKVSG MLHNTGQSL VFKVDKDTK QHVNISGGP 
      LAYRYQFEE IYIHYGVEN QKGSEHHIH GYSFPAEIQ 
      LYGFNKQLY HNMSEAQHK SQGIVGISL MVQIGDRSN 
      PEFRIITNV FSTVMYKGA STPIKHLSL KNMLPDTEH 
      FMTYEGSTT HPGCWETTV WIILNKPIY ITRQEMYQL 
      RKLMQGSKE APKAPLGNN ARPVQPLHQ RTIRTNINF 
      KNAVNGNCP SMHKEMYYK VNKWTDNDK GLHSPANWH 
      LRH
    CDS sequence
    • >TC008308 RA
      atgcatgcc acaacgtgc ttcgttctt cttgtcacc 
      tccttcttg tatgtggga tgtgacaac tggcaggaa 
      tggtggacc tacgacggg atttcgggt ccgggctac 
      tggggtctt atgaacccc cagtggaat atgtgttcg 
      aagggaaga agacagtct cccatcaac gtagagccg 
      gagaaattg ctgttcgac cctcacctg caacgcatc 
      cacttggac aaacacaaa gtgagcggc atgctgcac 
      aacacggga caatcgctg gtcttcaaa gtcgacaaa 
      gacaccaag cagcacgtg aacatatcg ggagggccg 
      ctggcctat cgctaccag ttcgaggag atttatata 
      cattacgga gtcgaaaat caaaaagga tcagaacac 
      cacattcac ggatattcg tttccggcg gagatccag 
      ctctacggg ttcaataag caactctat cacaacatg 
      tccgaagca caacacaag tctcagggc atcgtgggc 
      atatcctta atggtgcag atcggcgac aggtccaat 
      ccggaattc aggataatc accaacgtt ttcagcaca 
      gtgatgtac aaaggcgct tccacaccg ataaaacat 
      ttatcttta aaaaatatg ctgccagac acagagcac 
      ttcatgaca tatgaaggc tcgacgacg catcctggc 
      tgttgggag actactgtt tggatcatc cttaataag 
      cccatttat attactaga caggagatg taccaatta 
      aggaagctg atgcagggt tcgaaagaa gcgcccaaa 
      gccccattg ggcaacaac gcccgtcct gtccagccg 
      ctccatcaa agaaccatc agaaccaac atcaacttc 
      aagaacgcc gtgaacggc aactgtcct tctatgcac 
      aaggagatg tattacaaa gttaataaa tggactgac 
      aatgacaag gggctacac tcgccggcg aactggcat 
      ttgcgacac tga
    mRNA sequence
    • >TC008308 RA
      tccgggcgc acaattcca aaccgactc cggcagttt 
      gcgaccgac ctctgtctg caaggggtg ttcgcagcc 
      cgcactctg aacaaactc tcacgcgct ttgctatga 
      tcattcgga gctccagag gcatttatg acgggcttg 
      ttcggcatt gcttgtcaa catttgact tgaattagt 
      ttgtatatc ctgaaagat gtgttgctg actcggcga 
      cgagatgca tgccacaac gtgcttcgt tcttcttgt 
      cacctcctt cttgtatgt gggatgtga caactggca 
      ggaatggtg gacctacga cgggatttc gggtccggg 
      ctactgggg tcttatgaa cccccagtg gaatatgtg 
      ttcgaaggg aagaagaca gtctcccat caacgtaga 
      gccggagaa attgctgtt cgaccctca cctgcaacg 
      catccactt ggacaaaca caaagtgag cggcatgct 
      gcacaacac gggacaatc gctggtctt caaagtcga 
      caaagacac caagcagca cgtgaacat atcgggagg 
      gccgctggc ctatcgcta ccagttcga ggagattta 
      tatacatta cggagtcga aaatcaaaa aggatcaga 
      acaccacat tcacggata ttcgtttcc ggcggagat 
      ccagctcta cgggttcaa taagcaact ctatcacaa 
      catgtccga agcacaaca caagtctca gggcatcgt 
      gggcatatc cttaatggt gcagatcgg cgacaggtc 
      caatccgga attcaggat aatcaccaa cgttttcag 
      cacagtgat gtacaaagg cgcttccac accgataaa 
      acatttatc tttaaaaaa tatgctgcc agacacaga 
      gcacttcat gacatatga aggctcgac gacgcatcc 
      tggctgttg ggagactac tgtttggat catccttaa 
      taagcccat ttatattac tagacagga gatgtacca 
      attaaggaa gctgatgca gggttcgaa agaagcgcc 
      caaagcccc attgggcaa caacgcccg tcctgtcca 
      gccgctcca tcaaagaac catcagaac caacatcaa 
      cttcaagaa cgccgtgaa cggcaactg tccttctat 
      gcacaagga gatgtatta caaagttaa taaatggac 
      tgacaatga caaggggct acactcgcc ggcgaactg 
      gcatttgcg acactgagc tgcaccaga accggctct 
      tgtatataa tcgacgaac taggagacg cattttgtg 
      ataacgtat gcaataatg accaagtgg aggtgcgaa 
      aacgcggag ctttccgcc cgggatgtt aagtgtgta 
      gcagggcgt gagcattag gcattttgt gtgacgatt 
      atacaataa caaaccgtg tccaggtaa tgaataagg 
      caaacagat attttatta tttaacaag aaaattaat 
      tacgctaat aagagttaa attgtctgc agaagcaaa 
      tacttatat tcatttcaa tcttgtctg tatgtcagc 
      ttggacgaa atgaaactc gaagtctga gaataatcg 
      ggcatcagc tggcactta attaaagta atgcccacc 
      tcggagccc ttgattcac tgagactgt gacaaactg 
      gcaaacttc gagataatc ccgccagaa tttcttttc 
      gaattaaaa tagtgatcc acctaaatc ttcaaattt 
      aattccaga ctttttcga aacatattt catcccgca 
      attgcgaac tctcaccca ctcgaatga ttaaaagca 
      aatctgtgt ttaaataca catgtgaaa cattagact 
      aaatcagaa acaactata taactggaa ttataccgt 
      tatgcaggg taacttgta ctaaaccta ttacaaaca 
      tggcgaccc ttactaact taaagacaa acattccaa 
      acaaagcag ccgaaacaa ccagccaac atttaacgc 
      tcaacagtg catcactta ttcataaac aaattttat 
      ttagaaatg cacgaaacg gtttaatat ttcaataat 
      gacctctcg tttttaatt tgactagtt ctcagactt 
      cgaaagcgt taaatttta gcaatgaat ttgtatgct 
      tcgtaagta gcacattaa tgtatattg ccaaattgt 
      aaaatatag attaataca aaaataaaa tttattaac 
      aaaa