Status
Please wait ...
Gene information
General information
TC008276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7657655..7661959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008276
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01330

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008276 RA
      MMSFSRTIG LFFLFLVQA HSLRLVNDA AVLYLTFKE 
      NSIDLAVNL GEEVKISGE IGFNTSVTS EGNCNSSGC 
      SFKSDDEDF IVGFIDSGF SIYWESSNL EKEFRDCLD 
      LNGSNTNWF GGPEKWQAD WPIEKTVID NRLYTTLKT 
      DNGAVAERY WLNSKGGYA FVTDRVPLY LDQNKIRED 
      SVCFIASAT PPYINRTKV VLEYNLVVK PDPKEAQLH 
      AVNNFLGKP TEHPNEWIV RHPIWTTWA KYKAAINDS 
      IVVSFAQDI ADHGFVGQI EIDDKWEDC YGSLTFSED 
      TFPDINQTV QTLKDLNFT VTIWTHPFV NDDCAETVT 
      YGQEHGYFV IDDSGSDET SWWNGQAHH LDFSKQEAA 
      KWFTDRLKL LQEKHGIDG FKFDAGESD YAPWHPIVS 
      GDPELSPNS LSGDYIRTD AAFGGLTEV RTGWRTQDL 
      PIFLRMLDK DSRWTLNNG LYSLITSLL LFNINGYVF 
      VLPDMIGGN GYTEKPTAE LMVRWTQAN TFMPAMQFS 
      YLPWEITSD EFDSLEITK KFVALHADY ADAIIEAMN 
      NSIATGAPV NPPIWWVDP TNPDALKAN DEYLLGENI 
      LVAPVIQEG ATSRDIVLP TGSWKDGNS ETVYKGPQI 
      LKGYAAPID TLPYFIRQ
    CDS sequence
    • >TC008276 RA
      atgatgagt ttcagtcga acaattgga ctatttttt 
      ctttttttg gtgcaagct catagcttg cgcctagtc 
      aatgacgcc gccgttttg tacttgact tttaaagaa 
      aattcaatt gatttagcc gtaaatttg ggcgaagaa 
      gtaaaaatt tcgggtgaa atcggtttc aatacaagc 
      gttacttct gaagggaat tgtaacagt tcgggttgt 
      tcttttaaa agcgacgat gaagatttt atcgttggg 
      ttcatcgac agcggtttt agtatttac tgggaaagc 
      agtaacttg gaaaaagaa tttcgggat tgtttagac 
      ttgaatgga agcaataca aattggttt ggtggtccc 
      gaaaaatgg caagctgac tggcctata gaaaaaaca 
      gttattgat aacagactt tataccact cttaaaacc 
      gacaatggg gctgttgcc gagcggtat tggttgaat 
      tctaaaggt ggttatgct tttgtcaca gacagggtt 
      cccttgtat ttggatcaa aacaagatt agagaagac 
      agtgtttgt tttatagca tctgcgact cccccttat 
      attaacaga actaaggtc gttttggag tataatctt 
      gtggttaaa ccggaccct aaggaagcc caattacat 
      gcagttaac aactttttg ggcaaaccg acagaacat 
      cctaatgaa tggattgtt cggcatccc atttggacc 
      acttgggct aaatacaaa gctgcaata aacgacagt 
      atcgtggtg tcattcgcg caagacatt gccgatcat 
      gggtttgta ggccaaatc gaaattgac gacaaatgg 
      gaggactgt tacggttct ttaacattt agtgaagac 
      acatttccc gacataaat caaacagtt cagacttta 
      aaagatttg aattttact gttaccata tggacacat 
      cctttcgtc aatgatgat tgtgcagaa actgtaact 
      tatggccaa gaacatggt tatttcgtg attgatgat 
      tctggtagc gacgagact tcctggtgg aatggccaa 
      gcccatcat cttgatttt tcgaaacaa gaagcagca 
      aaatggttt actgatcgc ttaaaattg ctgcaagag 
      aagcacgga attgacggt tttaaattc gatgcgggt 
      gaaagtgac tatgctccc tggcaccct attgttagc 
      ggtgatcca gaattgagc cccaattct ctatcgggc 
      gattatatc agaactgat gctgctttt ggcggcttg 
      actgaagtt aggactggt tggaggacc caagacttg 
      ccaattttt ctcagaatg cttgacaaa gactcaagg 
      tggacgtta aacaacggt ctttattct ttaataaca 
      agtcttttg ttatttaat atcaatggt tacgtattt 
      gttttgcct gatatgatt ggtgggaat ggttacacc 
      gaaaaacca acggccgag ctgatggtg cggtggacg 
      caagctaac actttcatg cctgcgatg caatttagt 
      tacctccct tgggaaata accagcgat gagttcgat 
      agtcttgaa ataacaaaa aaattcgtt gctttgcat 
      gcggattac gcggatgct attatcgag gcaatgaat 
      aatagcatc gcaactggc gctccagtt aacccaccc 
      atttggtgg gttgaccca actaatcca gatgcactg 
      aaagcgaat gatgagtat ttgctgggg gaaaacatt 
      cttgtggct cctgtcatc caggaaggt gccactagt 
      cgcgatatt gtgctccca accgggtct tggaaagac 
      gggaattcg gagacagtt tacaaaggt ccgcaaatt 
      ctcaaaggt tatgcggct ccaatcgac actctccca 
      tatttcata agacaataa
    mRNA sequence
    • >TC008276 RA
      tcgttccta agtaagact tccgtttag ataccctta 
      caagaattt gccgttggt aaaagtatt cttattcgc 
      ttttatgtc tatattcag acttacttt acaaacacg 
      aaacttaat cgtttgcag tttttatcg ttattgctc 
      agataagtg ctaatttac tagtttcac tggtaacta 
      tttattagt cataaattt aggtgtgta aaatttacc 
      aagcaaagt atttagtca ttttatctt tttccaagt 
      taagtgtta acttaggta ggtgttctc atttgttat 
      tttttaaat ttctgtaaa gagatttta tagcgaaaa 
      atgaaaata gctggtgtt ggtgttttt ttactgctt 
      ttaatgttg gtgatggag aacgataca agttcgcaa 
      acaaatcga aataatgtt attctttgg ggagtacca 
      ggagggctt caatataat aatttcata cgaagatac 
      tcgctcaga tgcccgttt aggcaaaga tacataagc 
      ggcaaatgc agaagaatt tattttact tagaaataa 
      ttttttttg tcaatgatg agtttcagt cgaacaatt 
      ggactattt tttcttttt ttggtgcaa gctcatagc 
      ttgcgccta gtcaatgac gccgccgtt ttgtacttg 
      acttttaaa gaaaattca attgattta gccgtaaat 
      ttgggcgaa gaagtaaaa atttcgggt gaaatcggt 
      ttcaataca agcgttact tctgaaggg aattgtaac 
      agttcgggt tgttctttt aaaagcgac gatgaagat 
      tttatcgtt gggttcatc gacagcggt tttagtatt 
      tactgggaa agcagtaac ttggaaaaa gaatttcgg 
      gattgttta gacttgaat ggaagcaat acaaattgg 
      tttggtggt cccgaaaaa tggcaagct gactggcct 
      atagaaaaa acagttatt gataacaga ctttatacc 
      actcttaaa accgacaat ggggctgtt gccgagcgg 
      tattggttg aattctaaa ggtggttat gcttttgtc 
      acagacagg gttcccttg tatttggat caaaacaag 
      attagagaa gacagtgtt tgttttata gcatctgcg 
      actccccct tatattaac agaactaag gtcgttttg 
      gagtataat cttgtggtt aaaccggac cctaaggaa 
      gcccaatta catgcagtt aacaacttt ttgggcaaa 
      ccgacagaa catcctaat gaatggatt gttcggcat 
      cccatttgg accacttgg gctaaatac aaagctgca 
      ataaacgac agtatcgtg gtgtcattc gcgcaagac 
      attgccgat catgggttt gtaggccaa atcgaaatt 
      gacgacaaa tgggaggac tgttacggt tctttaaca 
      tttagtgaa gacacattt cccgacata aatcaaaca 
      gttcagact ttaaaagat ttgaatttt actgttacc 
      atatggaca catcctttc gtcaatgat gattgtgca 
      gaaactgta acttatggc caagaacat ggttatttc 
      gtgattgat gattctggt agcgacgag acttcctgg 
      tggaatggc caagcccat catcttgat ttttcgaaa 
      caagaagca gcaaaatgg tttactgat cgcttaaaa 
      ttgctgcaa gagaagcac ggaattgac ggttttaaa 
      ttcgatgcg ggtgaaagt gactatgct ccctggcac 
      cctattgtt agcggtgat ccagaattg agccccaat 
      tctctatcg ggcgattat atcagaact gatgctgct 
      tttggcggc ttgactgaa gttaggact ggttggagg 
      acccaagac ttgccaatt tttctcaga atgcttgac 
      aaagactca aggtggacg ttaaacaac ggtctttat 
      tctttaata acaagtctt ttgttattt aatatcaat 
      ggttacgta tttgttttg cctgatatg attggtggg 
      aatggttac accgaaaaa ccaacggcc gagctgatg 
      gtgcggtgg acgcaagct aacactttc atgcctgcg 
      atgcaattt agttacctc ccttgggaa ataaccagc 
      gatgagttc gatagtctt gaaataaca aaaaaattc 
      gttgctttg catgcggat tacgcggat gctattatc 
      gaggcaatg aataatagc atcgcaact ggcgctcca 
      gttaaccca cccatttgg tgggttgac ccaactaat 
      ccagatgca ctgaaagcg aatgatgag tatttgctg 
      ggggaaaac attcttgtg gctcctgtc atccaggaa 
      ggtgccact agtcgcgat attgtgctc ccaaccggg 
      tcttggaaa gacgggaat tcggagaca gtttacaaa 
      ggtccgcaa attctcaaa ggttatgcg gctccaatc 
      gacactctc ccatatttc ataagacaa taagcattg 
      ttaataaat aaaaaatcc tgtattgta ttaagacaa 
      taaacacgt ggtttatca aggtgaaaa acattttat 
      tttcgttct gtggattcg gcataatcg aaaaggtta 
      aaagttatc gcataatgt gcgtatttt ttgataacc 
      gcacttaca aggaaaatg tcgggctac tacaaggtt 
      atcaccgta gaattccca tcttcggca atgagctgc 
      aacaacttt ttaccacac aaattctat ttcacaatt 
      gtaacaaaa tgaacactc gtttttact ccgtaataa 
      ttgtactaa gtttgtcga tactcagtt cggtaagtt 
      ctccaataa ctcaaaaat cggttagtg ccaacacca 
      ccaccgaaa ttaatggct ggcatatcg acgaagaaa 
      agaagacga agaaaattg acggaaaac gactctttg 
      acgtaaagc cgaattgtg gaggaacat ttgtcaaaa 
      cattgggaa cttgcattt ctggatcca ccttcaatg 
      ttaattaaa taaattgtc tttgacgaa acgcaaatt 
      ttgtagttt ggttagtat gatatcaaa tgagctttg 
      tgcgggtca attttgtat tatctcgaa cgtgttatt 
      gtgaaaata ttccttacc taatataaa aacttgtga 
      tttgtgatg agccaacat gtggtattt ccttaaact 
      aactttcct ctttcaaat cgcgctgtt tatgttttt 
      tataagagc attggctag tcctgccga caagcctga 
      aagcgacca agtcgaaaa ctctgaaat cacgactcc 
      gagggaaag taagtaatg acactgtac aagtgttta 
      ttgcaaggt ttgttttta gtgcaaaga caatatgcc 
      attttcaca cccagatgt tcacagatt agactcaat 
      aagaggtgt cgatcgttt tggcatgat aacacaaat 
      aataattga ttatggaac ctgttttta ataaaataa 
      gtttaagta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY