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Gene information
General information
TC008247 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 7211973..7213134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008247
      iBeetle screen iB_01327
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008247 RA
        MFLVFLIAL FTSVRSVDL PEPKFQVLT PDELMVEVP 
        HYGEAKLVM FRIGVRRKD QNEEYLELG ENDVSNHRI 
        FIYDQRLNL QKDDVVNYL LAFRDKKGY EARTGNYVF 
        DGVYCKKPR RNRFRRSIS TTTVRAEFN LDGPDPDTE 
        EAFPVEPRF GHHPHRGGG WPNHGPHHG FNPFGPHRP 
        HYPPFSPYP NQPPPFGPP PNFGGDCND HRHDRCHGR 
        DTERIEGST ETSVDYHCK MCKYGSSER TSTTTVAST 
        TEDSPEINI RFSD
      CDS sequence
      • >TC008247 RA
        atgttcttg gtttttttg atcgcgctc ttcaccagt 
        gtcaggagt gttgacttg ccggagcca aagtttcaa 
        gtcctcact ccggacgag ttgatggtc gaagtgccc 
        cattatggc gaggcaaag ttggtgatg ttccggata 
        ggtgttaga cgcaaagac cagaatgag gagtatcta 
        gaactgggg gaaaacgac gtcagcaac caccggatt 
        ttcatttac gaccagcgg ctcaacttg cagaaagac 
        gatgtcgtg aattactta ctcgcgttt cgagataaa 
        aagggttac gaggcacga acgggaaac tacgttttt 
        gatggagtt tattgcaag aagcctcgt cgtaaccgc 
        ttccgtcgc tcgatttcg acaacgacc gtcagagcc 
        gagtttaat ctcgacggc cctgatcct gacaccgag 
        gaggccttt ccggtggag ccccgcttt gggcaccat 
        cctcaccga ggtgggggc tggccgaac cacggcccc 
        catcacggg ttcaaccct ttcgggccg caccggccg 
        cactatccc ccgttttcg ccctacccc aaccaaccg 
        ccacctttt ggacctccc ccgaacttc gggggtgac 
        tgcaatgac catcggcat gaccggtgc cacggacgc 
        gacaccgaa aggatcgag ggctccact gagacaagt 
        gtggactac cactgcaaa atgtgcaag tacggcagt 
        tccgaaagg acttccacc acaactgtg gcgagtacg 
        actgaagac tcgcccgaa ataaatatt aggttttct 
        gactag
      mRNA sequence
      • >TC008247 RA
        tcggtacga tatgttctt ggttttttt gatcgcgct 
        cttcaccag tgtcaggag tgttgactt gccggagcc 
        aaagtttca agtcctcac tccggacga gttgatggt 
        cgaagtgcc ccattatgg cgaggcaaa gttggtgat 
        gttccggat aggtgttag acgcaaaga ccagaatga 
        ggagtatct agaactggg ggaaaacga cgtcagcaa 
        ccaccggat tttcattta cgaccagcg gctcaactt 
        gcagaaaga cgatgtcgt gaattactt actcgcgtt 
        tcgagataa aaagggtta cgaggcacg aacgggaaa 
        ctacgtttt tgatggagt ttattgcaa gaagcctcg 
        tcgtaaccg cttccgtcg ctcgatttc gacaacgac 
        cgtcagagc cgagtttaa tctcgacgg ccctgatcc 
        tgacaccga ggaggcctt tccggtgga gccccgctt 
        tgggcacca tcctcaccg aggtggggg ctggccgaa 
        ccacggccc ccatcacgg gttcaaccc tttcgggcc 
        gcaccggcc gcactatcc cccgttttc gccctaccc 
        caaccaacc gccaccttt tggacctcc cccgaactt 
        cgggggtga ctgcaatga ccatcggca tgaccggtg 
        ccacggacg cgacaccga aaggatcga gggctccac 
        tgagacaag tgtggacta ccactgcaa aatgtgcaa 
        gtacggcag ttccgaaag gacttccac cacaactgt 
        ggcgagtac gactgaaga ctcgcccga aataaatat 
        taggttttc tgactagtg tcttgtatt accgttttt 
        taataaagt attttcatg gaagtcaag gctcaagta 
        ttgtgttag caaatcgca aaaacatga attatacat 
        gttgtttca ggagagatt caattatgt ttttttaaa 
        taatactgt ttactttac acacatatg aatatttaa 
        acctcggac gaattaaaa ttgtgttac aaaatctaa 
        atagatttt ccgcttagc agcgctcaa gtgtagcaa 
        ttcaagaac tccatgttt tgattggtt taataaaac 
        tgaaacttg aat