Status
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Gene information
General information
TC008246 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7204893..7209919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008246
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01326

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothoracic leg not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008246 RA
      MSIPQSDVE RRKQISVRG IAQVGDVNE IKKTFNRHL 
      HYTLVKDRN VATARDYYF ALAHTVKDH LVSRWIRTQ 
      QYYYEKDPK RVYYLSLEF YMGRSLSNT MINLGIQNS 
      VDEALYQLG LDIEELEEL EEDAGLGNG GLGRLAACF 
      LDSMATLGM AAYGYGIRY EYGIFAQKI INGEQQEEP 
      DDWLRFGNP WEKARPEYM LPVNFYGNV VDTPNGKKW 
      VNTQVIFAL PYDSPIPGY NNNVVNTLR LWSAKSPID 
      FNLKFFNDG DYIQAVLDR NLAENISRV LYPNDNNFG 
      GKELRLKQE YFMCAATLQ DIIRRFKAA KFGTREYTR 
      TDFDLLPDK VAIQLNDTH PSLAIPELM RILVDIEGL 
      PWNKAWNIT VKTCAYTNH TVLPEALER WPVEMLQNI 
      LPRHLEIIY QINHLHLEE VLKKWPGDL DRMRRMSLI 
      EEEGGKRVN MAHLSIVGS HAVNGVARI HSEIIKSDL 
      FKDFYEMTP EKFQNKTNG ITPRRWLLM CNPGLSDLI 
      SDKIGEDWV VHLDQLSKL TQWAKDPNF QRAVMKVKQ 
      ENKLKLAQL LEKEYGVKI NPGSMFDIH VKRIHEYKR 
      QLLNCLHII TLYNRIKRN PSAKCTPRT VMIGGKAAP 
      GYYTAKKII KLINCVANV VNNDPMVGD KLKVIFLEN 
      YRVSLAEKI IPAADLSEQ ISTAGTEAS GTGNMKFQL 
      NGALTIGTL DGANVEMAE EMGRENIFI FGMTVEEVE 
      ELKRKGYNA YDYYNANPE LKLVVDQIQ NGFFTPNNP 
      DEFKDLADI LLKYDRFFL LADYDAYIK KQEEASNVY 
      QDQAKWVEM AIHNIASSG KFSSDRTII EYGREIWGV 
      EPSYEKLPA PHEPRELTL KEN
    CDS sequence
    • >TC008246 RA
      atgtccatc ccccaatcg gacgtcgaa agacgcaaa 
      caaatcagt gtccgaggg atcgcccaa gtgggcgac 
      gtcaacgaa atcaagaag accttcaac aggcatctt 
      cactacacc ctcgtcaag gacaggaat gttgccact 
      gcaagagat tactacttc gctttggcg cacacggtg 
      aaggaccat ttggtctcg agatggatc aggacgcag 
      cagtattac tacgagaag gaccccaag agagtatac 
      tacctctcg ttggaattc tacatgggc cgctctctc 
      tccaacaca atgatcaac ctcgggatc caaaattcc 
      gtggacgag gctctctat cagctaggt ttggacatc 
      gaagagttg gaggaattg gaggaggac gcgggcttg 
      ggtaacggc ggcttgggc cgactggcg gcttgtttc 
      ctggactcg atggcgact ctagggatg gccgcctat 
      ggctacggc atccgctac gagtacggg attttcgcc 
      caaaaaatc ataaacggg gagcaacag gaggagccc 
      gacgattgg ctccggttc ggcaacccg tgggagaaa 
      gcccggcct gagtacatg ctccctgtt aatttttac 
      ggcaatgtt gtcgacacc ccgaatggg aaaaaatgg 
      gtcaacacc caagtgatt ttcgccctg ccgtatgat 
      agtcccatc cccggctat aacaacaac gttgtcaac 
      acgctgcgc ttgtggtcg gcgaaatcg cccattgat 
      tttaatctc aaattcttc aacgacggt gattatatt 
      caagccgtc ctcgaccgt aatctagct gaaaacatc 
      tcgcgtgtg ttgtacccc aatgacaat aatttcggg 
      gggaaagag ctgcgtctg aaacaggag tatttcatg 
      tgtgctgcc accctccaa gacattatc cggcgcttc 
      aaagcggcc aagttcggc actcgcgag tacacaaga 
      actgacttc gatttgtta cccgataag gttgccatt 
      caactgaat gatacgcac ccttctttg gctattccc 
      gaattgatg cgcattttg gtcgacatt gagggactc 
      ccctggaat aaagcgtgg aatatcacg gtcaaaacg 
      tgcgcttat accaaccat accgtacta cctgaagct 
      ctagagcgg tggccggtg gaaatgctc caaaatatt 
      ttaccgaga catttggag atcatctac caaattaat 
      cacttacac cttgaggag gttttgaaa aaatggcct 
      ggggacctt gacaggatg cgccgcatg tctctgatt 
      gaggaagag ggcgggaaa cgagtgaat atggcccat 
      ttgtccatt gtggggtca catgcagtg aacggggtc 
      gcaagaatt cactcagag attatcaag agcgatttg 
      tttaaggat ttctacgaa atgactccg gaaaagttc 
      cagaacaag accaacgga atcaccccc cgccgatgg 
      ctccttatg tgtaacccc ggcctgagc gacttgatt 
      tcggataaa atcggcgag gactgggtc gtccacctc 
      gaccagctc tccaagttg acccaatgg gccaaagac 
      cccaacttc caaagggcc gtgatgaag gtcaagcag 
      gaaaataag ctaaaactc gcccaactc ctagagaag 
      gaatacggc gtgaaaata aaccccggt tcgatgttc 
      gacatccac gtcaaacgc atacacgag tacaagcgg 
      caactgttg aactgcctc cacattatc acgctttat 
      aacagaatc aagcggaac ccttcagcc aaatgcacc 
      cccaggacg gtcatgatc gggggaaaa gcggcgccg 
      ggttattac accgcgaaa aaaatcatc aaactgatc 
      aactgtgtg gccaatgtt gtgaacaac gaccccatg 
      gttggggat aagttgaag gtgattttc ctggagaac 
      tatcgcgtg tcacttgcc gagaaaata atcccagct 
      gcggattta agcgagcag atttcgacc gctgggacg 
      gaggcgtca gggaccgga aatatgaag ttccagcta 
      aatggggcc ttgaccatt gggacgctg gacggagcg 
      aatgtggaa atggccgag gagatgggt cgcgagaat 
      atttttatt ttcgggatg acggttgaa gaagtggaa 
      gagttgaaa cggaagggt tacaacgcg tacgattac 
      tacaacgcg aaccccgaa ctgaaactt gtggttgac 
      cagattcag aacgggttc ttcactccg aataatccc 
      gatgagttt aaagacctg gcggatatt ttgctgaag 
      tacgatcgg ttcttttta ctggctgat tatgatgct 
      tatattaag aaacaggag gaagcgagt aatgtctat 
      caggatcaa gccaagtgg gttgaaatg gccattcac 
      aacatcgca tcgtcgggg aaattctcc agcgatcgg 
      accattatt gaatatggg cgggagata tggggcgtt 
      gaacctagt tatgagaaa ctcccagcg ccccacgaa 
      ccacgagaa ttgaccctt aaggagaac taa
    mRNA sequence
    • >TC008246 RA
      gtgaccttt acactcaaa ttgaactta gtaaagccg 
      gcgaatcga caacttctt cattacaat tcggtcgct 
      tccaagaaa tcaacatgt ccatccccc aatcggacg 
      tcgaaagac gcaaacaaa tcagtgtcc gagggatcg 
      cccaagtgg gcgacgtca acgaaatca agaagacct 
      tcaacaggc atcttcact acaccctcg tcaaggaca 
      ggaatgttg ccactgcaa gagattact acttcgctt 
      tggcgcaca cggtgaagg accatttgg tctcgagat 
      ggatcagga cgcagcagt attactacg agaaggacc 
      ccaagagag tatactacc tctcgttgg aattctaca 
      tgggccgct ctctctcca acacaatga tcaacctcg 
      ggatccaaa attccgtgg acgaggctc tctatcagc 
      taggtttgg acatcgaag agttggagg aattggagg 
      aggacgcgg gcttgggta acggcggct tgggccgac 
      tggcggctt gtttcctgg actcgatgg cgactctag 
      ggatggccg cctatggct acggcatcc gctacgagt 
      acgggattt tcgcccaaa aaatcataa acggggagc 
      aacaggagg agcccgacg attggctcc ggttcggca 
      acccgtggg agaaagccc ggcctgagt acatgctcc 
      ctgttaatt tttacggca atgttgtcg acaccccga 
      atgggaaaa aatgggtca acacccaag tgattttcg 
      ccctgccgt atgatagtc ccatccccg gctataaca 
      acaacgttg tcaacacgc tgcgcttgt ggtcggcga 
      aatcgccca ttgatttta atctcaaat tcttcaacg 
      acggtgatt atattcaag ccgtcctcg accgtaatc 
      tagctgaaa acatctcgc gtgtgttgt accccaatg 
      acaataatt tcgggggga aagagctgc gtctgaaac 
      aggagtatt tcatgtgtg ctgccaccc tccaagaca 
      ttatccggc gcttcaaag cggccaagt tcggcactc 
      gcgagtaca caagaactg acttcgatt tgttacccg 
      ataaggttg ccattcaac tgaatgata cgcaccctt 
      ctttggcta ttcccgaat tgatgcgca ttttggtcg 
      acattgagg gactcccct ggaataaag cgtggaata 
      tcacggtca aaacgtgcg cttatacca accataccg 
      tactacctg aagctctag agcggtggc cggtggaaa 
      tgctccaaa atattttac cgagacatt tggagatca 
      tctaccaaa ttaatcact tacaccttg aggaggttt 
      tgaaaaaat ggcctgggg accttgaca ggatgcgcc 
      gcatgtctc tgattgagg aagagggcg ggaaacgag 
      tgaatatgg cccatttgt ccattgtgg ggtcacatg 
      cagtgaacg gggtcgcaa gaattcact cagagatta 
      tcaagagcg atttgttta aggatttct acgaaatga 
      ctccggaaa agttccaga acaagacca acggaatca 
      ccccccgcc gatggctcc ttatgtgta accccggcc 
      tgagcgact tgatttcgg ataaaatcg gcgaggact 
      gggtcgtcc acctcgacc agctctcca agttgaccc 
      aatgggcca aagacccca acttccaaa gggccgtga 
      tgaaggtca agcaggaaa ataagctaa aactcgccc 
      aactcctag agaaggaat acggcgtga aaataaacc 
      ccggttcga tgttcgaca tccacgtca aacgcatac 
      acgagtaca agcggcaac tgttgaact gcctccaca 
      ttatcacgc tttataaca gaatcaagc ggaaccctt 
      cagccaaat gcaccccca ggacggtca tgatcgggg 
      gaaaagcgg cgccgggtt attacaccg cgaaaaaaa 
      tcatcaaac tgatcaact gtgtggcca atgttgtga 
      acaacgacc ccatggttg gggataagt tgaaggtga 
      ttttcctgg agaactatc gcgtgtcac ttgccgaga 
      aaataatcc cagctgcgg atttaagcg agcagattt 
      cgaccgctg ggacggagg cgtcaggga ccggaaata 
      tgaagttcc agctaaatg gggccttga ccattggga 
      cgctggacg gagcgaatg tggaaatgg ccgaggaga 
      tgggtcgcg agaatattt ttattttcg ggatgacgg 
      ttgaagaag tggaagagt tgaaacgga agggttaca 
      acgcgtacg attactaca acgcgaacc ccgaactga 
      aacttgtgg ttgaccaga ttcagaacg ggttcttca 
      ctccgaata atcccgatg agtttaaag acctggcgg 
      atattttgc tgaagtacg atcggttct ttttactgg 
      ctgattatg atgcttata ttaagaaac aggaggaag 
      cgagtaatg tctatcagg atcaagcca agtgggttg 
      aaatggcca ttcacaaca tcgcatcgt cggggaaat 
      tctccagcg atcggacca ttattgaat atgggcggg 
      agatatggg gcgttgaac ctagttatg agaaactcc 
      cagcgcccc acgaaccac gagaattga cccttaagg 
      agaactaaa tttgatatt ttgttataa attttcctg 
      tgggaacgt ttgatattg ttactttgt agaaacaaa 
      aagagtatt tacatgttg tataaatga aattgttgt 
      ttattaaaa cagtgccaa aattgttat tatgagctg 
      ttttgcggc gcttctgac actttattg ctctcacat 
      tatcttttg ttattatta ttattttta tgtaaaggt 
      tagtttgtt taagtattt tgaataaag tcaagtgtg 
      tcgtattat ttatggcac tgtgttcaa tatattgtt 
      gtttcattt atcttcctc tctttgaat cgttattcc 
      aataaatca gtaaaaaat at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY