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Gene information
General information
TC008241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 7147213..7151790
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008241
    iBeetle screen iB_01324
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 0 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008241 RA
      MALFKVQAL YDFVGESNT AELSITTGE VLTVTRTDI 
      GEGWWEGLN SRGQSGLFP EAYVAKIEE TAPPNMPPP 
      ALPPQNDWT GAQQDDDWD DEWDNDDTY SEIANNAQR 
      EPHYTNEPN QNQFAYGSG QVDNISESS YNVDNRVTK 
      KSLNIFSSY VKSGLESYI FGTLKDIPS HTKIRIFRE 
      NDNVFRWEL ISDPYTVQV TSPKKASKM GGLKSFIAY 
      QLTPSFTGI EVSRRYKHF DWLHDRLSE KFNIIAIPP 
      LPDKQISGR YEEHFIEHR RVQLQEFVN YVCRHPVLS 
      TCDVWRHFL TCTNEKQWK EGKRKAEKD QLVGANFCL 
      CIEAPDKDL LASTIESKI ESSISYINQ MDQCIKGLM 
      QTASDQQKK CENLYKREH TKIGESFFQ LGVAFEHRE 
      GGLFSDLAT DVKKIGTTY IEIAKLCED QAKIDWQPL 
      TDKLYLYRG ITSSYPDIF SMQKHAEQK KKECERNTQ 
      FSHQQITEV RKRTDVLTY SVFAELDHF QAEMNTEFR 
      SAMRSFLQE QVNYYKNVV SRLEDTLRK FE
    CDS sequence
    • >TC008241 RA
      atggcccta ttcaaagtc caagcactt tacgatttc 
      gtcggcgag tccaatact gccgagctc tcaattacg 
      accggcgaa gtgctaacg gttacgaga actgacatc 
      ggcgagggc tggtgggaa ggactgaac tcgcggggg 
      cagtcgggg ctcttcccc gaggcctat gtggccaag 
      atagaggag acggcgccc ccgaacatg ccgcccccg 
      gccctgccg ccgcagaac gactggacg ggggctcag 
      caagacgac gattgggac gatgagtgg gacaatgat 
      gacacatac agcgagatc gctaataat gcacaaagg 
      gagccccat tacactaac gagcccaac cagaatcag 
      ttcgcctat gggagcgga caagtggat aatatttcg 
      gaaagtagc tataatgtg gataacaga gtcaccaag 
      aagagttta aatattttc tcgtcgtat gtgaaaagt 
      ggattagaa agctatatt ttcggcacg ttaaaagac 
      atcccatcc cacacaaaa attcgaata tttcgagaa 
      aacgacaac gtgttccga tgggaactg atctcggac 
      ccgtacacg gtccaagtg acctcacca aaaaaagcg 
      tcaaaaatg ggcggcctc aaatcattc atcgcatat 
      caattaaca ccatcgttc accggaatc gaagtatcc 
      cgccgttac aaacacttc gactggctg cacgaccgc 
      ctctccgaa aagttcaac ataatcgca attcctccg 
      ctaccggat aaacagatt tcgggccgg tatgaggag 
      catttcatc gagcaccgg agagtgcag ctgcaggag 
      tttgtcaat tacgtgtgt cgacatcca gttctgtcg 
      acttgcgac gtttggagg cattttctc acctgcact 
      aacgaaaaa cagtggaag gaaggcaag agaaaagcg 
      gagaaagac cagttagtc ggggcgaac ttttgcctg 
      tgcatcgag gccccagat aaggacttg ttagcgtcg 
      actattgaa agtaaaatt gagagtagc atatcgtac 
      attaaccaa atggaccag tgcatcaag ggcttgatg 
      cagacggcg tccgatcag cagaaaaaa tgcgagaat 
      ttgtataaa agagaacat acgaaaatt ggcgagagc 
      tttttccag ctcggggtc gcgttcgag cacagggaa 
      ggtggattg ttctcggat ttggccaca gatgtgaag 
      aaaattggg accacttat attgaaatc gccaagttg 
      tgcgaagat caggcgaaa atcgattgg cagccgcta 
      accgataag ttgtacctc tacaggggg atcacgagt 
      tcgtacccc gatattttc tcgatgcaa aaacacgcc 
      gaacaaaag aagaaggag tgtgaaagg aacacgcag 
      ttctcacac cagcaaatc actgaagtt aggaagcgc 
      accgatgtt ctgacttac tcggttttc gccgaactc 
      gaccatttc caggctgag atgaacacc gaatttcgg 
      tcagccatg cggtcgttt ttgcaagaa caggtcaac 
      tattacaaa aatgtagtg agcagattg gaagacacc 
      ttgaggaag tttgagtag
    mRNA sequence
    • >TC008241 RA
      tcgcagtaa ccacctgca gttgtcagg aaagtgatg 
      acgatcgct aaagataaa taaatccgc ctaaaaaca 
      tcgtacaac ttccacttt cctcgaaaa acgagctta 
      aattgccat ccgtgcgcg aaaagtgcc ttaaacttg 
      ggtgtggtg ttgtgcgtt caactgtca atttatagg 
      ttatccaag tgtcatcgt gaaagttca aagcctccc 
      acttaatgc caccgtctg acatttccg agtgaatcc 
      cttcgtaat ggccctatt caaagtcca agcacttta 
      cgatttcgt cggcgagtc caatactgc cgagctctc 
      aattacgac cggcgaagt gctaacggt tacgagaac 
      tgacatcgg cgagggctg gtgggaagg actgaactc 
      gcgggggca gtcggggct cttccccga ggcctatgt 
      ggccaagat agaggagac ggcgccccc gaacatgcc 
      gcccccggc cctgccgcc gcagaacga ctggacggg 
      ggctcagca agacgacga ttgggacga tgagtggga 
      caatgatga cacatacag cgagatcgc taataatgc 
      acaaaggga gccccatta cactaacga gcccaacca 
      gaatcagtt cgcctatgg gagcggaca agtggataa 
      tatttcgga aagtagcta taatgtgga taacagagt 
      caccaagaa gagtttaaa tattttctc gtcgtatgt 
      gaaaagtgg attagaaag ctatatttt cggcacgtt 
      aaaagacat cccatccca cacaaaaat tcgaatatt 
      tcgagaaaa cgacaacgt gttccgatg ggaactgat 
      ctcggaccc gtacacggt ccaagtgac ctcaccaaa 
      aaaagcgtc aaaaatggg cggcctcaa atcattcat 
      cgcatatca attaacacc atcgttcac cggaatcga 
      agtatcccg ccgttacaa acacttcga ctggctgca 
      cgaccgcct ctccgaaaa gttcaacat aatcgcaat 
      tcctccgct accggataa acagatttc gggccggta 
      tgaggagca tttcatcga gcaccggag agtgcagct 
      gcaggagtt tgtcaatta cgtgtgtcg acatccagt 
      tctgtcgac ttgcgacgt ttggaggca ttttctcac 
      ctgcactaa cgaaaaaca gtggaagga aggcaagag 
      aaaagcgga gaaagacca gttagtcgg ggcgaactt 
      ttgcctgtg catcgaggc cccagataa ggacttgtt 
      agcgtcgac tattgaaag taaaattga gagtagcat 
      atcgtacat taaccaaat ggaccagtg catcaaggg 
      cttgatgca gacggcgtc cgatcagca gaaaaaatg 
      cgagaattt gtataaaag agaacatac gaaaattgg 
      cgagagctt tttccagct cggggtcgc gttcgagca 
      cagggaagg tggattgtt ctcggattt ggccacaga 
      tgtgaagaa aattgggac cacttatat tgaaatcgc 
      caagttgtg cgaagatca ggcgaaaat cgattggca 
      gccgctaac cgataagtt gtacctcta cagggggat 
      cacgagttc gtaccccga tattttctc gatgcaaaa 
      acacgccga acaaaagaa gaaggagtg tgaaaggaa 
      cacgcagtt ctcacacca gcaaatcac tgaagttag 
      gaagcgcac cgatgttct gacttactc ggttttcgc 
      cgaactcga ccatttcca ggctgagat gaacaccga 
      atttcggtc agccatgcg gtcgttttt gcaagaaca 
      ggtcaacta ttacaaaaa tgtagtgag cagattgga 
      agacacctt gaggaagtt tgagtagtt tttcttgcc 
      ttgttatca tctatcgtg tttatttcc aagccaaaa 
      caattgtga tttaacttt ttattatat aatctgtta 
      tatgtttat ccttacgtg ctgtgtata ttatggaaa 
      ttccaacgt ttacattaa tttttattt aatagaatc 
      accgagaca tattttgta catgaaatg taattgtat 
      atttctata tattttgtt aagtcagat gtaataaat 
      gtgcttaca cgaa