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Gene information
General information
TC008223 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6968910..6970652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008223
    iBeetle screen iB_01321

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008223 RA
      MDKGKQTNA PSKGGKVDG DGLTKKERR ALKVQEKNK 
      QHPPDNKKD SQNAPAMKT EPAKPANVP PGQLSEKEL 
      RRLEWEKKL AEQKPKPEK SENDNISKA ELKARRREQ 
      QEAQRLAKQ QQQKPPEKA AEVKKAAPS PKKVEKKKV 
      SDSRRLQIV SHLYSDASF SNASVYKNV HPAFVRLGV 
      QYSTKKILG SNARARALL AAVKCLIND LQTPPKQEF 
      CRYLETVLQ TCMDYLQLC RPLAVSMTN ALRHFKTKL 
      HQVECLEDN VKKEKLLEE IETYVAEEI DKAGEAISL 
      KVNEKISNG DVILTYGCS SLVTKILKD AHLAGKQFK 
      VVVIDGRPL LEGRTMLQR LVQIGINCT YVQMNAVSY 
      VMNQATKLL LGAHALLTN GYVMSRIGT AQVALVAQA 
      FSKPVLVCC ETYKFSEQV LTDSFVYNE LGDPEMLFT 
      ANTLDESSP LVTSKNDNL QILNLLYDV TPPDLITAV 
      VTELAILPC TSAPVVLRI KP
    CDS sequence
    • >TC008223 RA
      atggacaaa gggaagcaa actaacgcc ccatctaaa 
      gggggcaaa gtggatggc gatggtttg acaaaaaaa 
      gaacgccgg gcgctgaaa gtgcaagag aaaaataag 
      caacacccc cctgataac aaaaaagac agccaaaat 
      gcccctgct atgaaaacc gaacccgca aagcccgct 
      aatgttccc ccaggtcag ctcagcgaa aaagaattg 
      cgacgtttg gagtgggaa aaaaagctc gcggaacaa 
      aaacctaaa cctgaaaaa tccgaaaat gacaacata 
      agcaaagca gagttaaaa gcgagacga cgtgaacaa 
      caagaggca caaagacta gccaaacaa caacagcaa 
      aaacctcct gaaaaagct gcagaagtt aaaaaagct 
      gcaccttcg ccgaaaaaa gtcgaaaaa aagaaagtt 
      tcggattca agaagactc cagattgtg agtcattta 
      tattctgat gcaagtttc agtaatgcc agtgtttac 
      aaaaatgtt catccagcg tttgtaaga ctgggggtg 
      cagtactcg acgaaaaag attctaggg tcgaatgca 
      cgtgctcgg gcgcttctt gcagctgtt aaatgtctg 
      ataaatgat ctacaaacg cctccaaaa caggaattt 
      tgtcggtat ttggagact gtgcttcag acttgtatg 
      gattatctg caactttgt cgccctctt gccgtgtct 
      atgaccaac gctttgagg cattttaag acgaaactg 
      caccaagtg gagtgtctt gaagacaat gtaaagaag 
      gaaaaactt ttggaggaa attgaaacg tatgttgcg 
      gaggagatc gacaaagcg ggcgaagcg ataagtctc 
      aaagtcaat gagaaaatc agtaatggg gacgttatc 
      ctcacttac ggctgctca tctcttgtt accaaaatt 
      ttaaaggac gcccattta gccggcaaa caattcaaa 
      gttgtagta attgacggc cgccccctt ctcgaaggc 
      cgcacaatg ttacaacgt ttggtccaa attggaatc 
      aactgtact tacgttcaa atgaacgca gttagttat 
      gtaatgaac caagccacc aaactactc ctgggagcg 
      cacgcttta ctcacaaac ggttatgtt atgtcccgg 
      attggcacc gcacaagtg gctctagtg gcccaagct 
      ttcagtaaa ccggtcctg gtttgttgc gaaacttac 
      aaatttagt gaacaagtt ttaaccgat tcattcgtt 
      tacaatgaa ttaggtgac cctgagatg ctttttacc 
      gcaaacacg cttgatgag agtagcccc cttgttaca 
      tcaaagaat gacaattta cagattttg aacttgttg 
      tatgatgtt accccaccg gatttaatt acagcggtt 
      gttacagaa ttggcaatc ttaccttgc accagcgct 
      ccggttgtt ctaagaatt aagccctaa
    mRNA sequence
    • >TC008223 RA
      tcaaaaata taaatattt atgacatat atagcctca 
      aattcagca tatgtgtaa catttgcat taaaagggt 
      gataaatca ggcacaaaa tggacaaag ggaagcaaa 
      ctaacgccc catctaaag ggggcaaag tggatggcg 
      atggtttga caaaaaaag aacgccggg cgctgaaag 
      tgcaagaga aaaataagc aacaccccc ctgataaca 
      aaaaagaca gccaaaatg cccctgcta tgaaaaccg 
      aacccgcaa agcccgcta atgttcccc caggtcagc 
      tcagcgaaa aagaattgc gacgtttgg agtgggaaa 
      aaaagctcg cggaacaaa aacctaaac ctgaaaaat 
      ccgaaaatg acaacataa gcaaagcag agttaaaag 
      cgagacgac gtgaacaac aagaggcac aaagactag 
      ccaaacaac aacagcaaa aacctcctg aaaaagctg 
      cagaagtta aaaaagctg caccttcgc cgaaaaaag 
      tcgaaaaaa agaaagttt cggattcaa gaagactcc 
      agattgtga gtcatttat attctgatg caagtttca 
      gtaatgcca gtgtttaca aaaatgttc atccagcgt 
      ttgtaagac tgggggtgc agtactcga cgaaaaaga 
      ttctagggt cgaatgcac gtgctcggg cgcttcttg 
      cagctgtta aatgtctga taaatgatc tacaaacgc 
      ctccaaaac aggaatttt gtcggtatt tggagactg 
      tgcttcaga cttgtatgg attatctgc aactttgtc 
      gccctcttg ccgtgtcta tgaccaacg ctttgaggc 
      attttaaga cgaaactgc accaagtgg agtgtcttg 
      aagacaatg taaagaagg aaaaacttt tggaggaaa 
      ttgaaacgt atgttgcgg aggagatcg acaaagcgg 
      gcgaagcga taagtctca aagtcaatg agaaaatca 
      gtaatgggg acgttatcc tcacttacg gctgctcat 
      ctcttgtta ccaaaattt taaaggacg cccatttag 
      ccggcaaac aattcaaag ttgtagtaa ttgacggcc 
      gcccccttc tcgaaggcc gcacaatgt tacaacgtt 
      tggtccaaa ttggaatca actgtactt acgttcaaa 
      tgaacgcag ttagttatg taatgaacc aagccacca 
      aactactcc tgggagcgc acgctttac tcacaaacg 
      gttatgtta tgtcccgga ttggcaccg cacaagtgg 
      ctctagtgg cccaagctt tcagtaaac cggtcctgg 
      tttgttgcg aaacttaca aatttagtg aacaagttt 
      taaccgatt cattcgttt acaatgaat taggtgacc 
      ctgagatgc tttttaccg caaacacgc ttgatgaga 
      gtagccccc ttgttacat caaagaatg acaatttac 
      agattttga acttgttgt atgatgtta ccccaccgg 
      atttaatta cagcggttg ttacagaat tggcaatct 
      taccttgca ccagcgctc cggttgttc taagaatta 
      agccctaaa tcacttttg aaattaata aatgacaaa 
      atgatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY