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Gene information
General information
TC008216 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6893400..6898283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008216
    iBeetle screen iB_01319

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially spotted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 25.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 25.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008216 RA
      MTDQDNAVN GLEPKEASA SNIEVLKDL CENNANFFA 
      KDTSENGQR LYISFMGIR ENLDEISQK MARIASEAP 
      RFDFDESTP GNGYRSFLS VANSAIDYG IALNKKICL 
      KRASAFFRK SQLTKDVES CAHLIASLD TCFKHLETL 
      ISWSSAGNL FPDEEHSPQ ELLSNYEDI NQYCFYGRC 
      MGFQFCESL RNILQFISM SMAIFSEIY YNQGSFFAK 
      ATTSLMATS KYVNDPEQR ARRIVNISQ NADVNFCKA 
      FWFLSENEL MTNMPYVVA PSVAVSRII SLPPEPFTL 
      EVDGREIDV PVPSSHIGR KPVQVRLIS HEVHEGMIG 
      EASNKYKSK PPARGLIIH CHGGGFVAQ SSLSHECYL 
      REWAKQLKV PILSIDYSL APEAPFPRA LEEVTYAYC 
      WALKNCQLL GSTGERIIG AGDSAGANL LLSTTLKCL 
      NLGIPPPMG LFVAYVPTL VNFVPSPSR LLCMMDPLL 
      PFGFMMRCL KAYACPDNK KVENCDQTS DTESFEEIS 
      ESDLMELQA HKSPVSETS DTLTYGSLN SGEDDRNKD 
      IDLQSSNIE NSQKYVSDF LEKYVLDSD TETDGTRVP 
      VLKHESATS SQSIDDSLQ TKVSSFVSN LKWRFNSWI 
      AQKAQVGVD FMVPRSASA AEKYLDLDR QGNLLDEFK 
      FTVPEDPFL SPFCAKDED LERFPPVRV LTVHLDPCL 
      DDCVMFSKR LKQLNKNVH LDILSGLPH GFLNFSLIS 
      KEAYEGSKL CVKRINELL NLDNLPPLQ
    CDS sequence
    • >TC008216 RA
      atgacagat caagataat gcagtaaac ggcctagaa 
      cccaaggaa gcaagtgcc tcgaacatc gaggtgctg 
      aaagacctg tgcgagaac aacgcgaat ttttttgcc 
      aaagacacg agcgagaat gggcaacgg ctgtatata 
      tccttcatg gggattagg gagaacttg gatgagatc 
      tcccagaag atggcacga attgcgtca gaagccccc 
      aggtttgat tttgatgaa agcaccccc gggaacggg 
      tatcggagt ttcctgagc gttgccaac tcggcgatt 
      gattatggc atagcgttg aacaagaag atctgtttg 
      aaacgtgcc agtgctttc ttcaggaag tcacaacta 
      acaaaggat gtggagtcc tgtgcccac ttaattgca 
      agtctggac acgtgtttc aaacatttg gaaacgctg 
      atttcctgg agcagtgca ggaaatttg tttccagat 
      gaggagcat tcgcctcaa gaactattg tccaactac 
      gaggacatc aaccaatat tgcttctac gggcgttgt 
      atggggttt caattttgt gaatcattg agaaacatt 
      ttacaattt atctcaatg tcaatggcg attttttcg 
      gaaatttat tacaaccaa gggtctttt ttcgcaaaa 
      gccacgacc tccctcatg gccacatca aaatacgtc 
      aatgacccg gaacaaagg gccagaaga attgtcaat 
      atatcgcaa aatgccgat gtaaatttc tgcaaagct 
      ttttggttc ctttcggaa aatgagttg atgacaaac 
      atgccgtat gtggtggcc ccaagtgtt gcagtttcc 
      cgaattatc agtttgccc cctgaaccg ttcactctt 
      gaggttgac gggcgcgaa attgatgtc cctgtccct 
      tcatctcat attggtaga aaaccagtc caagtgcgc 
      cttatcagt catgaagtc catgaaggc atgattggg 
      gaagcttca aataaatac aagagtaaa ccaccagct 
      aggggcctg attatacat tgtcatggg ggcggtttt 
      gttgcccag tcttcgtta tcacacgag tgttatttg 
      agggaatgg gcaaaacaa ctaaaggtc ccgattttg 
      agcattgat tacagttta gcgcctgaa gctcccttt 
      ccaagggct ttggaggag gtcacttat gcctattgt 
      tgggccttg aaaaactgt caattgcta ggaagcaca 
      ggtgagaga ataataggc gctggggat tcagctggc 
      gcaaacttg ctgctctcc accactcta aaatgccta 
      aatttgggc attccgccc ccgatgggc ctattcgta 
      gcatacgtg cctacttta gtgaatttc gttccttca 
      ccatctcgc ttattatgt atgatggat cctttgtta 
      ccgttcggt tttatgatg cgttgtttg aaagcttat 
      gcatgtcct gacaataaa aaagtggaa aactgcgac 
      caaacttcg gacactgaa agtttcgaa gaaatatcc 
      gaatcggac ttgatggag ttacaagct cacaaatca 
      ccagtgtct gaaacttcc gacaccttg acctacggg 
      tcattaaat tcaggagaa gacgacagg aataaagat 
      attgatttg caatcttct aatatcgag aatagtcaa 
      aaatacgtt tccgatttt ttggaaaaa tacgttttg 
      gattctgac actgaaact gacggaacg cgagtacct 
      gttttaaaa cacgaaagt gcgacttcg tcccagtca 
      attgacgat tcgttgcaa accaaagtt tcgagtttt 
      gtttcgaat ttaaagtgg cgattcaac agctggatt 
      gcacagaag gcgcaagtt ggggtcgat tttatggtc 
      ccgaggagt gcaagcgct gcggaaaaa tacctagat 
      ttagacaga cagggtaat ttactcgat gagttcaaa 
      tttacggtc cctgaagat ccatttttg agccctttt 
      tgtgcaaaa gatgaggat ttggagcga tttccacca 
      gttcgcgtt ttgaccgtt catttagac ccctgcttg 
      gatgattgc gtgatgttt tcgaagcga ctgaagcaa 
      ttaaataaa aacgtccac ttggatatt ttatcgggt 
      ttaccgcac ggattcttg aatttttct ttgatttcc 
      aaagaagcc tatgagggt tccaagctc tgtgtaaaa 
      agaatcaac gagctcttg aacttggat aatctgcct 
      ccactacaa taa
    mRNA sequence
    • >TC008216 RA
      tcatgctcc tgtcaccaa aatacaagt agaacttcg 
      cctaatttt taatgtaaa cattattta tctaatctc 
      agctaaatc tcaagctac atgaacaaa tacattaac 
      acgtaaaca aacaaaatt ttaaagtgt ctcgaaatg 
      acagatcaa gataatgca gtaaacggc ctagaaccc 
      aaggaagca agtgcctcg aacatcgag gtgctgaaa 
      gacctgtgc gagaacaac gcgaatttt tttgccaaa 
      gacacgagc gagaatggg caacggctg tatatatcc 
      ttcatgggg attagggag aacttggat gagatctcc 
      cagaagatg gcacgaatt gcgtcagaa gcccccagg 
      tttgatttt gatgaaagc acccccggg aacgggtat 
      cggagtttc ctgagcgtt gccaactcg gcgattgat 
      tatggcata gcgttgaac aagaagatc tgtttgaaa 
      cgtgccagt gctttcttc aggaagtca caactaaca 
      aaggatgtg gagtcctgt gcccactta attgcaagt 
      ctggacacg tgtttcaaa catttggaa acgctgatt 
      tcctggagc agtgcagga aatttgttt ccagatgag 
      gagcattcg cctcaagaa ctattgtcc aactacgag 
      gacatcaac caatattgc ttctacggg cgttgtatg 
      gggtttcaa ttttgtgaa tcattgaga aacatttta 
      caatttatc tcaatgtca atggcgatt ttttcggaa 
      atttattac aaccaaggg tcttttttc gcaaaagcc 
      acgacctcc ctcatggcc acatcaaaa tacgtcaat 
      gacccggaa caaagggcc agaagaatt gtcaatata 
      tcgcaaaat gccgatgta aatttctgc aaagctttt 
      tggttcctt tcggaaaat gagttgatg acaaacatg 
      ccgtatgtg gtggcccca agtgttgca gtttcccga 
      attatcagt ttgccccct gaaccgttc actcttgag 
      gttgacggg cgcgaaatt gatgtccct gtcccttca 
      tctcatatt ggtagaaaa ccagtccaa gtgcgcctt 
      atcagtcat gaagtccat gaaggcatg attggggaa 
      gcttcaaat aaatacaag agtaaacca ccagctagg 
      ggcctgatt atacattgt catgggggc ggttttgtt 
      gcccagtct tcgttatca cacgagtgt tatttgagg 
      gaatgggca aaacaacta aaggtcccg attttgagc 
      attgattac agtttagcg cctgaagct ccctttcca 
      agggctttg gaggaggtc acttatgcc tattgttgg 
      gccttgaaa aactgtcaa ttgctagga agcacaggt 
      gagagaata ataggcgct ggggattca gctggcgca 
      aacttgctg ctctccacc actctaaaa tgcctaaat 
      ttgggcatt ccgcccccg atgggccta ttcgtagca 
      tacgtgcct actttagtg aatttcgtt ccttcacca 
      tctcgctta ttatgtatg atggatcct ttgttaccg 
      ttcggtttt atgatgcgt tgtttgaaa gcttatgca 
      tgtcctgac aataaaaaa gtggaaaac tgcgaccaa 
      acttcggac actgaaagt ttcgaagaa atatccgaa 
      tcggacttg atggagtta caagctcac aaatcacca 
      gtgtctgaa acttccgac accttgacc tacgggtca 
      ttaaattca ggagaagac gacaggaat aaagatatt 
      gatttgcaa tcttctaat atcgagaat agtcaaaaa 
      tacgtttcc gattttttg gaaaaatac gttttggat 
      tctgacact gaaactgac ggaacgcga gtacctgtt 
      ttaaaacac gaaagtgcg acttcgtcc cagtcaatt 
      gacgattcg ttgcaaacc aaagtttcg agttttgtt 
      tcgaattta aagtggcga ttcaacagc tggattgca 
      cagaaggcg caagttggg gtcgatttt atggtcccg 
      aggagtgca agcgctgcg gaaaaatac ctagattta 
      gacagacag ggtaattta ctcgatgag ttcaaattt 
      acggtccct gaagatcca tttttgagc cctttttgt 
      gcaaaagat gaggatttg gagcgattt ccaccagtt 
      cgcgttttg accgttcat ttagacccc tgcttggat 
      gattgcgtg atgttttcg aagcgactg aagcaatta 
      aataaaaac gtccacttg gatatttta tcgggttta 
      ccgcacgga ttcttgaat ttttctttg atttccaaa 
      gaagcctat gagggttcc aagctctgt gtaaaaaga 
      atcaacgag ctcttgaac ttggataat ctgcctcca 
      ctacaataa ttttttata gatacaatt aggaaataa 
      atgggactc tagactagt tcgctctct caaaaacac 
      atttaaaaa tttctgtcc agtatataa agtattttt 
      tatacaaat ttaaggtgt gttgtaggt gttttcaat 
      taccaagta cacgtttta ttttgttaa ttgttcctg 
      aaaattatt tttagatac ctggatctg ttggttgcg 
      gctgtgttt aacttaacg tttttgctg ctacggtct 
      attatttgg tgcctaatc gatctttaa tccaaattt 
      agcagtaca tcaaatcat aagtccata gcagtatta 
      gaaaatgat tgtatttac atttcctta tataccaaa 
      gtgaacttg ttacgtaac tcatagtga taattattt 
      gtgcaagta gatctgatt gtgaacatt tatacaatg 
      tatttattg gattattaa cggaaataa attattaaa 
      caaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY