Status
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Gene information
General information
TC008212 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6800625..6814616
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008212
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01318

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 2
      mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008212 RA
        MKCIVSTAC VLSVLVAVS FAAPYFPAQ FSASSNLQI 
        GGGGGSTSV AASGTASTG TSTSGGGLS DILGSIDIP 
        GLLRSVNSI VKLVGVFCP PLSQVLDNV IQNVTSTAF 
        RVFGRVILR GGLGGGSGG SSGGSTASR VNVVLPTYP 
        PDEDEDYED EEATSPADN VDLRISQST SATQTGQTV 
        SAASDDENT VAKRHVRVV RAAEKEEAA EEEEEEDQD 
        GAESAPQPA EDLSNVDAD DTDRNKRYS PFGGAEHHG 
        GAGSGNFLF DIIRLVAGS GATEESDEK IGAGGVAEI 
        DIAKGAGVE HHGEGIPGP ITRLFVIAN RGLANLVQD 
        LILRLAQTS ERLVNFKAR LVTSLI
      • >TC008212 RB
        MKCIVSTAC VLSVLVAVS FAAPYFPAQ FSASSNLQI 
        GGGGGSTSV AASGTASTG TSTSGGGLS DILGSIDIP 
        GLLRSVNSI VKLVGVFCP PLSQVLDNV IQNVTSTAF 
        RVFGRVILR GGLGGGSGG SSGGSTASR VNVVLPTYP 
        PDEDEDYED EEATSPADN VDLRISQST SATQTGQTV 
        SAASDDENT VAKRHVRVV RAAEKEEAA EEEEEEDQD 
        GAESAPQPA EDLSNVDAD DTDRNKRYS PFGGAEHHG 
        GAGSGNFLF DIIRVSNGA
      CDS sequence
      • >TC008212 RA
        atgaagtgt atcgtaagt accgcgtgt gttttgagt 
        gttttggtt gctgttagt tttgccgcc ccatatttc 
        cccgcacag ttctcagcg tcctctaac ctccagata 
        ggaggaggc ggcggctct acgagtgtc gctgcaagt 
        ggtactgca tccactggc actagcacg tccggagga 
        ggtttatca gatatttta ggaagcatt gatattccg 
        ggcttgttg cgaagtgtg aacagtata gtgaaactt 
        gttggcgtc ttctgtccg ccgttatcg caggtgctc 
        gataacgtg attcagaac gtgaccagt acggcgttt 
        cgagtcttt ggccgtgtt atcctcaga ggcggcttg 
        ggtggagga agtggtggg agcagtggg ggtagtact 
        gcgtccaga gtcaatgtt gtcttgccc acttatcca 
        cccgacgaa gacgaagat tacgaggat gaggaagcg 
        acaagtccc gctgataat gtcgatttg aggatttct 
        cagtcgacc tctgccacc caaacgggc cagactgtg 
        tcagctgcc agcgacgac gaaaacacg gtcgcgaaa 
        aggcacgtg agggtggtt cgagcagca gaaaaggaa 
        gaagctgca gaggaagag gaagaggag gatcaagat 
        ggggccgaa tcggcaccg cagcccgct gaagaccta 
        tcgaacgtt gacgccgac gacaccgac cggaacaag 
        agatactct cccttcgga ggcgcggag catcatggc 
        ggagccgga tcaggaaat ttcttgttt gatataatc 
        cggctcgtc gccggatct ggggccact gaagaatcc 
        gatgagaag atcggcgct ggaggtgtt gcagagatc 
        gacattgcg aagggggct ggagtggag caccatggc 
        gagggaatc cccggaccc atcacccgt ttgtttgtc 
        attgctaat cgcggtctc gccaacctc gtacaagac 
        ttaatcttg cgtttggcc caaactagc gaaagatta 
        gtcaatttc aaggctcgt cttgttaca tctctcatc 
        taa
      • >TC008212 RB
        atgaagtgt atcgtaagt accgcgtgt gttttgagt 
        gttttggtt gctgttagt tttgccgcc ccatatttc 
        cccgcacag ttctcagcg tcctctaac ctccagata 
        ggaggaggc ggcggctct acgagtgtc gctgcaagt 
        ggtactgca tccactggc actagcacg tccggagga 
        ggtttatca gatatttta ggaagcatt gatattccg 
        ggcttgttg cgaagtgtg aacagtata gtgaaactt 
        gttggcgtc ttctgtccg ccgttatcg caggtgctc 
        gataacgtg attcagaac gtgaccagt acggcgttt 
        cgagtcttt ggccgtgtt atcctcaga ggcggcttg 
        ggtggagga agtggtggg agcagtggg ggtagtact 
        gcgtccaga gtcaatgtt gtcttgccc acttatcca 
        cccgacgaa gacgaagat tacgaggat gaggaagcg 
        acaagtccc gctgataat gtcgatttg aggatttct 
        cagtcgacc tctgccacc caaacgggc cagactgtg 
        tcagctgcc agcgacgac gaaaacacg gtcgcgaaa 
        aggcacgtg agggtggtt cgagcagca gaaaaggaa 
        gaagctgca gaggaagag gaagaggag gatcaagat 
        ggggccgaa tcggcaccg cagcccgct gaagaccta 
        tcgaacgtt gacgccgac gacaccgac cggaacaag 
        agatactct cccttcgga ggcgcggag catcatggc 
        ggagccgga tcaggaaat ttcttgttt gatataatc 
        cgggtaagc aacggggcg taa
      mRNA sequence
      • >TC008212 RA
        tcactgtac gatggtcgc tgatggtgg tggttttaa 
        agagagaat ggcgggaga aaagttgat attcgatta 
        gaaaactct cgggtgttc ggtgtcagt ttagttctt 
        ccttcgtgt ttgtgctgt gtgcatttg ttacttttt 
        tacaaagtg ttaacctag tctagtcaa aatgaagtg 
        tatcgtaag taccgcgtg tgttttgag tgttttggt 
        tgctgttag ttttgccgc cccatattt ccccgcaca 
        gttctcagc gtcctctaa cctccagat aggaggagg 
        cggcggctc tacgagtgt cgctgcaag tggtactgc 
        atccactgg cactagcac gtccggagg aggtttatc 
        agatatttt aggaagcat tgatattcc gggcttgtt 
        gcgaagtgt gaacagtat agtgaaact tgttggcgt 
        cttctgtcc gccgttatc gcaggtgct cgataacgt 
        gattcagaa cgtgaccag tacggcgtt tcgagtctt 
        tggccgtgt tatcctcag aggcggctt gggtggagg 
        aagtggtgg gagcagtgg gggtagtac tgcgtccag 
        agtcaatgt tgtcttgcc cacttatcc acccgacga 
        agacgaaga ttacgagga tgaggaagc gacaagtcc 
        cgctgataa tgtcgattt gaggatttc tcagtcgac 
        ctctgccac ccaaacggg ccagactgt gtcagctgc 
        cagcgacga cgaaaacac ggtcgcgaa aaggcacgt 
        gagggtggt tcgagcagc agaaaagga agaagctgc 
        agaggaaga ggaagagga ggatcaaga tggggccga 
        atcggcacc gcagcccgc tgaagacct atcgaacgt 
        tgacgccga cgacaccga ccggaacaa gagatactc 
        tcccttcgg aggcgcgga gcatcatgg cggagccgg 
        atcaggaaa tttcttgtt tgatataat ccggctcgt 
        cgccggatc tggggccac tgaagaatc cgatgagaa 
        gatcggcgc tggaggtgt tgcagagat cgacattgc 
        gaagggggc tggagtgga gcaccatgg cgagggaat 
        ccccggacc catcacccg tttgtttgt cattgctaa 
        tcgcggtct cgccaacct cgtacaaga cttaatctt 
        gcgtttggc ccaaactag cgaaagatt agtcaattt 
        caaggctcg tcttgttac atctctcat ctaagcaaa 
        ttacatcgc catcgtaat ttaatccac ctcgctcat 
        gcaacacct tcacgcatt gttaccact attgtaaaa 
        aacaagcat ggagctcat tattataaa ttaatttat 
        cagtttcta tttttgtta ttttttatt taaattttt 
        tgtatatag tgataactt tcttttcac tgttctttt 
        aacacgtca tcatgtggt cgtcttgcc atttcttcc 
        ttaaatttt gttattctt tttctactt cttcactag 
        ctttaaatt ctagcgtaa gtctctctg ttaaattta 
        gcttcgcag tataaatta ccagatgta tataacctg 
        aacaattgc tcagtttga acttactac atggaaatg 
        taaatagaa atttcttca tcatggtgt agattattg 
        tctattttg tgtctctgt aagtagtgt acataattt 
        aaaaggaaa cacaggttt aaaaatttt gattttttt 
        atgaaagtt actgtatgt tgtattttt tatagtaat 
        tatattaaa aatacaaaa ttctttaaa aaatgtcag 
        tgtatttat tagtttgag tggaactaa gtcatcatc 
        atcgtatat ggttcttgt tcactattt agtattagt 
        agtgtaatt aaataaaat gaacgctag ctt
      • >TC008212 RB
        gtgatcctc gcaaaattt caaaactct acaagtgtt 
        aacctagtc tagtcaaaa tgaagtgta tcgtaagta 
        ccgcgtgtg ttttgagtg ttttggttg ctgttagtt 
        ttgccgccc catatttcc ccgcacagt tctcagcgt 
        cctctaacc tccagatag gaggaggcg gcggctcta 
        cgagtgtcg ctgcaagtg gtactgcat ccactggca 
        ctagcacgt ccggaggag gtttatcag atattttag 
        gaagcattg atattccgg gcttgttgc gaagtgtga 
        acagtatag tgaaacttg ttggcgtct tctgtccgc 
        cgttatcgc aggtgctcg ataacgtga ttcagaacg 
        tgaccagta cggcgtttc gagtctttg gccgtgtta 
        tcctcagag gcggcttgg gtggaggaa gtggtggga 
        gcagtgggg gtagtactg cgtccagag tcaatgttg 
        tcttgccca cttatccac ccgacgaag acgaagatt 
        acgaggatg aggaagcga caagtcccg ctgataatg 
        tcgatttga ggatttctc agtcgacct ctgccaccc 
        aaacgggcc agactgtgt cagctgcca gcgacgacg 
        aaaacacgg tcgcgaaaa ggcacgtga gggtggttc 
        gagcagcag aaaaggaag aagctgcag aggaagagg 
        aagaggagg atcaagatg gggccgaat cggcaccgc 
        agcccgctg aagacctat cgaacgttg acgccgacg 
        acaccgacc ggaacaaga gatactctc ccttcggag 
        gcgcggagc atcatggcg gagccggat caggaaatt 
        tcttgtttg atataatcc gggtaagca acggggcgt 
        aatggattt ttccaaatg tgtggctat tttggatca 
        catcgagtg attttgtaa cttaaccta ctttctact 
        acaatttca aatatttcg gatcgagta gcagcactt 
        agttaagat gatcattta ggtttttaa gcgccattc 
        catttcttc acatttttg ttacacatt gacactgat 
        ttgtctcac gtctttaat tttaagttg atcattgta 
        ttaataaat tttttcaag taca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY