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Gene information
General information
TC008201 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6557795..6583599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008201
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01317

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008201 RA
      MHNLNVNLA STAVNSHNL LVDSACTTP RTPEILNSL 
      IAMTNPMDN YSYGNNNIA KTSSKPPTN MSSDSNSSS 
      SSQLESPTT NPPSVQQTC SQLIKAGLK LSIEQKRKH 
      QSDGEDLLD LDKCKRIKK NDCSESEED KNKQDGLTP 
      EDEERRRRR RERNKIAAT KCRLKKREK TANLVNESE 
      TLETQNIEL KSQLHELQN QKRGLIEML NLHRPVCQH 
      NISPVTREC IYRLPPVGS VIETHSYSR PASVDPNFR 
      TQNMEVYSR PASVGVTNN ITYSRPNMN FKAPSIVVE 
      EVNEAYPQL TNLDNISYH YNSQCHNYS SQSYTASGM 
      DNGCMA
    CDS sequence
    • >TC008201 RA
      atgcataac ttaaacgta aatctggcc tcgacggcc 
      gtcaactcc cacaacctc ttggtggac agtgcttgc 
      actacacca cgcactcct gaaatcctc aattcgctg 
      atagccatg acaaatccg atggataat tattcgtac 
      gggaataac aacatcgcg aaaacatcg tcgaagcct 
      cccacgaac atgtccagc gattccaac agctccagc 
      agtagtcaa ttagagtct cccacgacc aatccgccc 
      agcgtccaa caaacatgc tcccaactt atcaaggct 
      ggactcaaa ctctcgatc gagcaaaaa cgaaaacat 
      caaagcgac ggagaggat ctcctggac ctcgacaag 
      tgcaaacgc atcaagaag aacgactgc agcgagtca 
      gaggaggac aagaacaag caagacggg ttaacgccc 
      gaggacgag gagcgtcgg cgccgccgc cgcgagcgc 
      aacaaaatc gccgcgacg aaatgccgc ttgaagaag 
      cgcgaaaag accgccaac ctcgtcaac gaatctgaa 
      acgctggaa acgcaaaat atcgagctc aagagccaa 
      ctccacgag ctgcagaac cagaaacgc ggccttatc 
      gagatgctg aacctgcac cggcccgtg tgtcagcac 
      aacatctcc ccggtgacc agggagtgc atctaccgg 
      ctgcccccg gtggggtcc gtcatcgag acgcattcc 
      tacagccgg ccggcgtcc gtggacccc aacttcagg 
      acgcagaac atggaggtg tacagccgg cccgccagc 
      gtgggggtc accaacaac atcacctac agccgcccc 
      aacatgaac ttcaaagcc ccgagcatt gttgtcgag 
      gaggtgaat gaggcctat ccccagctg accaacctc 
      gacaacatc tcctaccac tacaatagc cagtgtcat 
      aactactcc tctcagagt tacacagcg tctggaatg 
      gacaacgga tgcatggct tag
    mRNA sequence
    • >TC008201 RA
      gatgtcaga ttcggccaa tagcgcggc gcgcgcatg 
      acatcaagg tctaaaaat aacgatgtt ataaggaaa 
      accgcacat gggagtgta ttatgtttg agtttagcg 
      gagcacgcg ggcgactta acttcgtga gaggcatat 
      gcgatgttt attattcac gctaatccg gattttgta 
      aaatcgtgt acgaagtgt cagagactg ttagttcgt 
      gataaatat aaactgatt tgtggttcg aatcctggc 
      tgtggcagg tggttttac gtgagattg ccttcgccg 
      aacccagtg gattgcctg aaccactcg cgagaagtg 
      gcccaaagg tgctaacag acaatattt ctaattctg 
      cgagtgtcg ctcgcgtct gattgtgat ttagtttgt 
      aaaaagtgt gaaaaatcg tgatattat gcataactt 
      aaacgtaaa tctggcctc gacggccgt caactccca 
      caacctctt ggtggacag tgcttgcac tacaccacg 
      cactcctga aatcctcaa ttcgctgat agccatgac 
      aaatccgat ggataatta ttcgtacgg gaataacaa 
      catcgcgaa aacatcgtc gaagcctcc cacgaacat 
      gtccagcga ttccaacag ctccagcag tagtcaatt 
      agagtctcc cacgaccaa tccgcccag cgtccaaca 
      aacatgctc ccaacttat caaggctgg actcaaact 
      ctcgatcga gcaaaaacg aaaacatca aagcgacgg 
      agaggatct cctggacct cgacaagtg caaacgcat 
      caagaagaa cgactgcag cgagtcaga ggaggacaa 
      gaacaagca agacgggtt aacgcccga ggacgagga 
      gcgtcggcg ccgccgccg cgagcgcaa caaaatcgc 
      cgcgacgaa atgccgctt gaagaagcg cgaaaagac 
      cgccaacct cgtcaacga atctgaaac gctggaaac 
      gcaaaatat cgagctcaa gagccaact ccacgagct 
      gcagaacca gaaacgcgg ccttatcga gatgctgaa 
      cctgcaccg gcccgtgtg tcagcacaa catctcccc 
      ggtgaccag ggagtgcat ctaccggct gcccccggt 
      ggggtccgt catcgagac gcattccta cagccggcc 
      ggcgtccgt ggaccccaa cttcaggac gcagaacat 
      ggaggtgta cagccggcc cgccagcgt gggggtcac 
      caacaacat cacctacag ccgccccaa catgaactt 
      caaagcccc gagcattgt tgtcgagga ggtgaatga 
      ggcctatcc ccagctgac caacctcga caacatctc 
      ctaccacta caatagcca gtgtcataa ctactcctc 
      tcagagtta cacagcgtc tggaatgga caacggatg 
      catggctta gcccctttc cagactttt cctgtactt 
      tactagtgt gaatatttt tatacttat gcatacggc 
      gggaattgc aatatgttt gccagccaa atgtgtggt 
      tctaaacct ccgtccact cacatccca cctcattta 
      ttggcgcat tgctttgtt ccaaattaa aatttgagc 
      acacactag gtaaaaaaa attgattct ttccctcaa 
      attttctac aatgaaatt aaaaaaaat cttttggtt 
      tttgccgag ttggcaggt cttgcctta tagtacttt 
      gttataggg gtctcaaaa aattatatt cattgcccc 
      atataaact taaactatt gggtgacta taaatatga 
      gttcaaaaa gccactcat aaaattaat tctttggta 
      tggattttt tttttaaat tctccaact ggtgatttt 
      atttttata atttttata cacttgaca tttcaaatt 
      tcatgatgt cacaagttt ttgagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY