Status
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Gene information
General information
TC008197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6501068..6503754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008197
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01316

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008197 RA
      MKLLKGLCV FLICATASA QDIDNNREN QEEGRGLGG 
      YNQEWRAVD YFSPTSDSS GWVPQSDTD DGTPKRRRI 
      RKRKRRPPV FTEDDWPKK TKEPWEEMS EENTQKTNT 
      RRRLTPIYE EKIRDPFTA LQEAEEKQK TKVDESSTK 
      TPDLKTILK NSGGLSLSE VLQQKNLSL TELLKGNRE 
      AISALAKEA ETTTEVYTY RRLPPSIGL KKDINRNFE 
      RESAEHLSS DEVVEAQRK RLALLHGHR DTEVTAGVP 
      AKAVTVSLD EILSVFKNE SDGPLKMTI DLDEMTVKK 
      PPTTEQSAG KKLKNVTAK EEIMEILQN PVERENLSR 
      ILRSRNMTV EELVQQRER GSSQLHLAD IFHNKTREP 
      EPQEEHVGH INPASFRKP KTIPEFPET TKPPYTITS 
      FPTYKIETN KNALPVWRQ LYPSFFPDV YQNSNQADK 
      KEIRPTTST EKLDYEEIE RLEEIENTL AEAANGRLK 
      VDVPQNVYE EEFLRMPAG VKSAILASL AIIGLSLLV 
      FLTILVVFK WTQKQKRRL NYSAAFAGS KIKSPILEP 
      EQRRTFKTF VCETLGRKK NNYYKSHLH SMSDSIWDS 
      EKKPYMI
    CDS sequence
    • >TC008197 RA
      atgaagtta ctcaaggga ctgtgtgtg tttctcatt 
      tgtgcgacc gcttccgcc caggatatt gataacaat 
      cgggaaaat caggaagaa ggaagagga cttggtggc 
      tacaaccag gaatggaga gcagtggat tatttctcg 
      cccacttct gacagctcc ggctgggtc ccacaatcc 
      gacaccgac gacggaacc cccaaacgg cgacggatc 
      cggaaacgg aaacgtcga ccccccgtc ttcaccgaa 
      gacgactgg cccaaaaaa accaaagag ccgtgggag 
      gaaatgagc gaggaaaac acccaaaaa accaacacc 
      cgcagacga ctcacgccc atctacgag gagaaaatc 
      cgcgacccc ttcacagcg ttacaagaa gccgaggaa 
      aaacagaag actaaagtg gacgaaagc tcgaccaaa 
      acgcccgat ttgaagaca attctcaaa aacagtggc 
      ggtttgagt ttgagtgag gttttgcaa cagaagaat 
      ttgtcgctg actgagttg ctcaaaggg aatcgggaa 
      gcgatttcc gctcttgcc aaagaagca gaaaccacg 
      actgaagtt tacacttat cgccggttg ccgccgtcc 
      atcggctta aagaaagac atcaaccgg aatttcgaa 
      cgcgaatcc gccgaacac ctgtcttca gacgaggtg 
      gttgaagca cagcgcaaa agactggcc cttttgcac 
      ggtcacagg gacacagaa gtgacagcg ggcgtgccc 
      gcgaaagcg gtcactgta agcctggac gaaattttg 
      agtgttttc aagaacgag tcggacggt ccgttgaaa 
      atgaccatt gatttggac gaaatgacg gttaaaaaa 
      ccgcccaca actgagcaa tccgcgggc aagaagctg 
      aaaaatgtg accgccaag gaggagatc atggaaatt 
      ttgcaaaac ccggtcgag cgtgaaaac ctatcccgg 
      attttgcgt tcgcgcaat atgacagtc gaggagctg 
      gtgcagcag cgggaacgt ggctccagt cagttgcac 
      ttggcggat attttccac aacaaaacg cgggagcct 
      gagccacaa gaggagcac gttgggcac attaaccct 
      gccagtttc cgcaagccc aagaccatt cccgagttt 
      ccggagacc acgaaaccg ccctacact atcaccagt 
      ttcccgact tacaaaatc gaaaccaac aaaaacgcc 
      cttccggtt tggcgccaa ctgtacccg tccttcttc 
      ccggatgtt taccagaac agtaatcag gctgataag 
      aaagaaata cggcctact acaagtacc gagaagtta 
      gattacgaa gaaatcgag cggctggag gagatcgag 
      aacacgctg gctgaagcg gccaacggg agactcaaa 
      gtggacgtg ccacagaac gtgtacgag gaggagttc 
      ctcaggatg ccagcgggg gtcaagtcg gcgatcctg 
      gccagcttg gccatcatc gggctgtct ctgctcgtg 
      tttttgacc attttggtg gtcttcaag tggacgcaa 
      aagcaaaaa cggagactg aactacagc gcggctttc 
      gccggctcg aagattaaa tcgccgatt ttggagccg 
      gagcaaagg cggactttt aagacgttc gtgtgcgaa 
      acgctcgga cgcaagaag aataattac tacaagtcg 
      catcttcac agcatgtcg gactcgatc tgggacagc 
      gagaagaag ccctacatg atctga
    mRNA sequence
    • >TC008197 RA
      tcgcagata taaaatttt aaacggctc tggtagtgt 
      tttctcgaa tttggagaa agccataga gcactagaa 
      cagtctttg attgacttc gccccccac tattggtga 
      aaggtttca gacgtgcgg agcacatgg taaattatc 
      actcgatac tacaaatcc agtgtctta gtgcagcat 
      gcgactgct gtccaaaca ttccgctaa agtattatg 
      aactgtgct tgagtggcg atgaagtta ctcaaggga 
      ctgtgtgtg tttctcatt tgtgcgacc gcttccgcc 
      caggatatt gataacaat cgggaaaat caggaagaa 
      ggaagagga cttggtggc tacaaccag gaatggaga 
      gcagtggat tatttctcg cccacttct gacagctcc 
      ggctgggtc ccacaatcc gacaccgac gacggaacc 
      cccaaacgg cgacggatc cggaaacgg aaacgtcga 
      ccccccgtc ttcaccgaa gacgactgg cccaaaaaa 
      accaaagag ccgtgggag gaaatgagc gaggaaaac 
      acccaaaaa accaacacc cgcagacga ctcacgccc 
      atctacgag gagaaaatc cgcgacccc ttcacagcg 
      ttacaagaa gccgaggaa aaacagaag actaaagtg 
      gacgaaagc tcgaccaaa acgcccgat ttgaagaca 
      attctcaaa aacagtggc ggtttgagt ttgagtgag 
      gttttgcaa cagaagaat ttgtcgctg actgagttg 
      ctcaaaggg aatcgggaa gcgatttcc gctcttgcc 
      aaagaagca gaaaccacg actgaagtt tacacttat 
      cgccggttg ccgccgtcc atcggctta aagaaagac 
      atcaaccgg aatttcgaa cgcgaatcc gccgaacac 
      ctgtcttca gacgaggtg gttgaagca cagcgcaaa 
      agactggcc cttttgcac ggtcacagg gacacagaa 
      gtgacagcg ggcgtgccc gcgaaagcg gtcactgta 
      agcctggac gaaattttg agtgttttc aagaacgag 
      tcggacggt ccgttgaaa atgaccatt gatttggac 
      gaaatgacg gttaaaaaa ccgcccaca actgagcaa 
      tccgcgggc aagaagctg aaaaatgtg accgccaag 
      gaggagatc atggaaatt ttgcaaaac ccggtcgag 
      cgtgaaaac ctatcccgg attttgcgt tcgcgcaat 
      atgacagtc gaggagctg gtgcagcag cgggaacgt 
      ggctccagt cagttgcac ttggcggat attttccac 
      aacaaaacg cgggagcct gagccacaa gaggagcac 
      gttgggcac attaaccct gccagtttc cgcaagccc 
      aagaccatt cccgagttt ccggagacc acgaaaccg 
      ccctacact atcaccagt ttcccgact tacaaaatc 
      gaaaccaac aaaaacgcc cttccggtt tggcgccaa 
      ctgtacccg tccttcttc ccggatgtt taccagaac 
      agtaatcag gctgataag aaagaaata cggcctact 
      acaagtacc gagaagtta gattacgaa gaaatcgag 
      cggctggag gagatcgag aacacgctg gctgaagcg 
      gccaacggg agactcaaa gtggacgtg ccacagaac 
      gtgtacgag gaggagttc ctcaggatg ccagcgggg 
      gtcaagtcg gcgatcctg gccagcttg gccatcatc 
      gggctgtct ctgctcgtg tttttgacc attttggtg 
      gtcttcaag tggacgcaa aagcaaaaa cggagactg 
      aactacagc gcggctttc gccggctcg aagattaaa 
      tcgccgatt ttggagccg gagcaaagg cggactttt 
      aagacgttc gtgtgcgaa acgctcgga cgcaagaag 
      aataattac tacaagtcg catcttcac agcatgtcg 
      gactcgatc tgggacagc gagaagaag ccctacatg 
      atctgagcg ttcaaacgt atttattgt tttatttat 
      gtgatttcg tgtgcaata tttattaag actttgcca 
      agttttgtt gttgtgtat ttttaagta cttgtatgt 
      tacaaaata aacactttt tagacat
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