| General information | |||
| TC031105
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6146528..6160177 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031105 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01314 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC031105 RA
MEMKAAKLR EELDELGYY LTFSHESVP LVEKLVADL KTTTQSLQK YMKIAQNAI EERDNLQLG AEPYRCDNA RLVKECNEL HQQFLRERE ENEKIQRDL KRRLESLKQ ENIDCLTER EKLKAKIKQ LEVEIVNCG EILLNPKQK PKPGLKSAS TTALVGQKS TRKAKNFEL NSAMALADQ KIANLSKDV KKLKEHNLQ LIEANEVLQ SQLNNRDKE IKRLSGLLE GGRPIQAIK KDCTCNCTC TNKDLHEGG SDQLNKVLQ EKHVLENRL KDALAKQHE AMKRCMHLA ERNKLLEKE MKDIDHLAL AVEAECNNT VKNNAEKVN RLQDRMQES LIQIQTLER ENNQLKQDT KELSEKLDA ITGEKKHLQ TVLETSLEE KKRLTDKIN NFTIIEHDL NMEIDRLNR LSAEQKKKI AELESLVDS KAHTEEAKR ITSDVSPSR GKPKIKKKP PKKPSKSGS PVQPSLQVP TNTPIDGSP GTGRCCNCH CDSCSSSKH LKELLDKEL EYREQQAAR CIDNLKSEK DYYMREYHK ILEQMKNVP SRPSSSRID KNNELVQKL SEQEQTLAL LEHKNRMLS KEKFDLISK LDSVRDLPD TSTDGPCLK ANCKRTERE RDLLRSDLE RIEEERDIL RSKLKNVSE GQINEQERL KKRLLDAED QIHKLEQER RELIQNQGT RRSAIDTLE EQCETLKNQ LRTAQNELN QQRALYNQL KTLHEQTDR SLSDTQSKL IQVESELAS ALENLKRRE QDRGTVSKE VDLLKNDIQ IMKSQLAQI DQEKDELLV ALDDKTEKL AMMEHELKS REKTISSLE SAVNEMKRK LGSSVDENA NQRHEIQSL ETELATMKQ ELDTIKKIK DSAIQENRR LQDDLGSVT CDCRDARKE LELYKRQVD DLKTQLQHY VAEVKRTED LISNKEIER NELLDQFRS LSHEANILE TNNHTLETE ATQSRVQLS VALDHAADL ERKLDNKDS IIHSYEKQI ADLTSQIAS LEIQLRQCM NHYDKADEE LQNMRDFSL KLESENARL KRQLRERDD QRMQIDRNV DQLRNEREF LQHTVTRDR RNVESVERD LHDAKHETT ELRILNRDL QEEVQNLKM QVQELKEKF VATSEQLDM YQEKALEYA QQNKQLRRE VANERFRRT RDSESSKYP SL
- >TC031105 RB
MEMKAAKLR EELDELGYY LTFSHESVP LVEKLVADL KTTTQSLQK YMKIAQNAI EERDNLQLG AEPYRCDNA RLVKECNEL HQQFLRERE ENEKIQRDL KRRLESLKQ ENIDCLTER EKLKAKIKQ LEVEIVNCG EILLNPKQK PKPGLKSAS TTALVGQKS TRKAKNFEL NSAMALADQ KIANLSKDV KKLKEHNLQ LIEANEVLQ SQLNNRDKE IKRLSGLLE GGRPIQAIK KDCTCNCTC TNKDLHEGG SDQLNKVLQ EKHVLENRL KDALAKQHE AMKRCMHLA ERNKLLEKE MKDIDHLAL AVEAECNNT VKNNAEKVN RLQDRMQES LIQIQTLER ENNQLKQDT KELSEKLDA ITGEKKHLQ TVLETSLEE KKRLTDKIN NFTIIEHDL NMEIDRLNR LSAEQKKKI AELESLVDS KAHTEEAKR ITSDVSPSR GKPKIKKKP PKKPSKSGS PVQPSLQVP TNTPIDGSP GTGRCCNCH CDSCSSSKH LKELLDKEL EYREQQAAR CIDNLKSEK DYYMREYHK ILEQMKNVP SRPSSSRID KNNELVQKL SEQEQTLAL LEHKNRMLS KEKFDLISK LDSVRDLPD TSTDGPCLK ANCKRTERE RDLLRSDLE RIEEERDIL RSKLKNVSE GQINEQERL KKRLLDAED QIHKLEQER RELIQNQGT RRSAIDTLE EQCETLKNQ LRTAQNELN QQRALYNQL KTLHEQTDR SLSDTQSKL IQVESELAS ALENLKRRE QDRGTVSKE VDLLKNDIQ IMKSQLAQI DQEKDELLV ALDDKTEKL AMMEHELKS REKTISSLE SAVNEMKRK LG
- >TC031105 RA
atggagatg aaagccgca aagctaagg gaagaatta gacgagctg ggttactac ctaacattt tcccacgaa tccgtcccc cttgtcgaa aaactcgtc gccgacctg aaaacaacg acccaaagc ctacaaaaa tacatgaaa atagcccaa aatgcaatc gaggaacga gacaatctt cagttaggt gcggagccc tatcggtgc gataatgcg cggttggtg aaagaatgt aacgagttg catcagcag tttttacgt gaaagggag gaaaatgag aaaattcaa agagattta aagaggcgt ttggagtcg ttgaaacaa gaaaacatt gactgtctt accgaacgg gagaagttg aaagctaaa atcaaacaa ttggaagtg gaaattgtg aattgtggg gaaattctc ttaaatccc aaacaaaaa cccaaacct gggctaaaa agtgcctca actactgcg ttagttggg cagaagtcg accagaaaa gcgaaaaat tttgagcta aacagcgcc atggctttg gcggaccaa aaaatcgcc aacttaagt aaagatgta aagaagttg aaggagcat aacttgcaa ttaattgaa gctaatgag gttttgcaa tcacagtta aataataga gataaggaa ataaagcgc ttaagtggc cttctggaa ggtggaagg cccattcaa gcaataaaa aaagattgt acgtgtaat tgcacttgc actaataaa gatttacat gagggtggt tcagatcaa ctgaacaag gttttgcaa gaaaaacac gttctggaa aatcgtctt aaagacgct ctcgctaaa caacacgaa gcaatgaaa cgttgcatg catttagcg gaaagaaac aaattgttg gagaaagaa atgaaagac attgaccat ttagcttta gctgtcgaa gctgagtgt aacaatacc gttaaaaac aacgcggag aaagttaat cgtttgcag gacaggatg caagaaagt cttattcaa attcaaact ttagaaaga gaaaacaat cagcttaaa caagacaca aaagaactg agtgaaaag ttggacgct attacggga gagaaaaaa catttacaa acggtttta gaaacgtcc ttggaggaa aaaaagcga ctgacggat aaaattaac aacttcacg ataattgaa catgatttg aatatggaa attgacaga ttgaatcgc ctttcagcc gaacaaaag aaaaaaatc gccgaattg gaatcatta gtggattca aaagcacac acagaggag gccaagcgt atcacttct gacgtgtcc ccaagtcgg ggtaaaccc aaaattaag aaaaaacct ccgaaaaaa ccatcgaaa agtggttct cccgttcaa ccatcactt caagtgccc acaaatacg cccattgat gggtctcct gggacaggc aggtgttgc aattgtcac tgcgattcc tgttcttcg tcaaaacat ctcaaagag ttgttggac aaagaactg gaatatagg gaacaacaa gctgctcgc tgtattgat aatttgaag tcagagaaa gattattat atgagagaa tatcataag attttagaa caaatgaaa aatgtgcct agtcggccg agtagtagt agaattgat aagaataat gaacttgtt caaaaattg tcggaacaa gaacagaca ttagctctc ttagaacac aaaaatagg atgttgtcg aaagaaaag tttgatttg atttcaaaa cttgatagt gtgagggat ttacctgat acaagtacg gacggtcct tgtctcaaa gccaattgt aagcgcacc gagcgtgag cgcgatctt ttgcgttca gatcttgaa agaattgaa gaagaacga gatattctc agatccaaa ttaaaaaat gtgtcagaa ggtcaaatt aacgaacaa gagagactg aaaaaacgt ctcttagat gcggaagac caaattcac aaattagaa caggaacgt cgtgaattg attcaaaat caaggaaca agaagatcg gcaattgat actcttgaa gaacaatgc gagactctt aaaaatcaa ttacgcaca gcacaaaac gaactgaat caacagagg gcgttatac aatcaatta aagactttg catgaacaa actgatcgc tcattatca gacactcag agtaaatta attcaagtg gaatcggaa ctcgctagt gccttagaa aatttgaaa cggcgggag caagatagg ggaacagtc agcaaagag gtggatttg ctgaaaaat gatattcag ataatgaaa agtcagttg gcacaaatt gaccaggag aaggacgag ttgctggtg gccttagat gacaagact gaaaagctc gcaatgatg gagcacgag ctgaaatcc cgcgaaaag acaatttct tcactcgaa tcggctgtg aatgaaatg aagagaaaa ctgggctcg tccgtcgac gagaatgcg aatcaaaga catgaaata caaagttta gaaacggaa ttagcgacc atgaaacag gaacttgac actattaaa aaaattaaa gacagtgcg attcaggag aatcgcaga cttcaggac gatttaggt tcggtcact tgtgactgt cgggacgct cgcaaggag ttagaattg tacaagaga caagtggat gatttgaaa actcagctc cagcattac gttgctgaa gttaaaaga accgaggat ctgatctcg aataaagag attgaaagg aacgagttg ttggatcag tttaggtcg ctcagtcat gaagccaat attttagaa actaacaat cacacttta gaaaccgaa gctacgcaa agtagagta caattgtcg gtcgcgttg gatcatgcc gccgatttg gaacgcaaa ctggacaat aaggactcg atcattcac agttatgag aaacaaatt gctgatttg accagtcaa attgcaagt ttggagatc cagctcaga cagtgcatg aatcattac gacaaagct gacgaagag ttgcaaaac atgcgggat ttttccttg aaattagag tcggaaaat gcgcgtttg aagcgacag ttgcgcgag agggacgat caaagaatg caaattgat cgaaacgtt gatcagttg agaaacgaa cgcgaattt ttacaacac acagtcacg cgagacaga cgcaatgtg gaatcggtt gaaagggat ttgcacgat gcgaagcac gaaaccacc gagttgaga atcttgaat cgggatctt caggaagaa gtgcaaaat ttgaaaatg caagtgcaa gaactaaag gaaaaattt gttgcaacg tccgaacag ttagacatg taccaagaa aaggcgcta gagtacgcc caacaaaac aaacagtta aggcgagag gtcgcaaat gagcgcttt cgcaggact agagatagc gaaagttcc aaatatccg tccctttaa
- >TC031105 RB
atggagatg aaagccgca aagctaagg gaagaatta gacgagctg ggttactac ctaacattt tcccacgaa tccgtcccc cttgtcgaa aaactcgtc gccgacctg aaaacaacg acccaaagc ctacaaaaa tacatgaaa atagcccaa aatgcaatc gaggaacga gacaatctt cagttaggt gcggagccc tatcggtgc gataatgcg cggttggtg aaagaatgt aacgagttg catcagcag tttttacgt gaaagggag gaaaatgag aaaattcaa agagattta aagaggcgt ttggagtcg ttgaaacaa gaaaacatt gactgtctt accgaacgg gagaagttg aaagctaaa atcaaacaa ttggaagtg gaaattgtg aattgtggg gaaattctc ttaaatccc aaacaaaaa cccaaacct gggctaaaa agtgcctca actactgcg ttagttggg cagaagtcg accagaaaa gcgaaaaat tttgagcta aacagcgcc atggctttg gcggaccaa aaaatcgcc aacttaagt aaagatgta aagaagttg aaggagcat aacttgcaa ttaattgaa gctaatgag gttttgcaa tcacagtta aataataga gataaggaa ataaagcgc ttaagtggc cttctggaa ggtggaagg cccattcaa gcaataaaa aaagattgt acgtgtaat tgcacttgc actaataaa gatttacat gagggtggt tcagatcaa ctgaacaag gttttgcaa gaaaaacac gttctggaa aatcgtctt aaagacgct ctcgctaaa caacacgaa gcaatgaaa cgttgcatg catttagcg gaaagaaac aaattgttg gagaaagaa atgaaagac attgaccat ttagcttta gctgtcgaa gctgagtgt aacaatacc gttaaaaac aacgcggag aaagttaat cgtttgcag gacaggatg caagaaagt cttattcaa attcaaact ttagaaaga gaaaacaat cagcttaaa caagacaca aaagaactg agtgaaaag ttggacgct attacggga gagaaaaaa catttacaa acggtttta gaaacgtcc ttggaggaa aaaaagcga ctgacggat aaaattaac aacttcacg ataattgaa catgatttg aatatggaa attgacaga ttgaatcgc ctttcagcc gaacaaaag aaaaaaatc gccgaattg gaatcatta gtggattca aaagcacac acagaggag gccaagcgt atcacttct gacgtgtcc ccaagtcgg ggtaaaccc aaaattaag aaaaaacct ccgaaaaaa ccatcgaaa agtggttct cccgttcaa ccatcactt caagtgccc acaaatacg cccattgat gggtctcct gggacaggc aggtgttgc aattgtcac tgcgattcc tgttcttcg tcaaaacat ctcaaagag ttgttggac aaagaactg gaatatagg gaacaacaa gctgctcgc tgtattgat aatttgaag tcagagaaa gattattat atgagagaa tatcataag attttagaa caaatgaaa aatgtgcct agtcggccg agtagtagt agaattgat aagaataat gaacttgtt caaaaattg tcggaacaa gaacagaca ttagctctc ttagaacac aaaaatagg atgttgtcg aaagaaaag tttgatttg atttcaaaa cttgatagt gtgagggat ttacctgat acaagtacg gacggtcct tgtctcaaa gccaattgt aagcgcacc gagcgtgag cgcgatctt ttgcgttca gatcttgaa agaattgaa gaagaacga gatattctc agatccaaa ttaaaaaat gtgtcagaa ggtcaaatt aacgaacaa gagagactg aaaaaacgt ctcttagat gcggaagac caaattcac aaattagaa caggaacgt cgtgaattg attcaaaat caaggaaca agaagatcg gcaattgat actcttgaa gaacaatgc gagactctt aaaaatcaa ttacgcaca gcacaaaac gaactgaat caacagagg gcgttatac aatcaatta aagactttg catgaacaa actgatcgc tcattatca gacactcag agtaaatta attcaagtg gaatcggaa ctcgctagt gccttagaa aatttgaaa cggcgggag caagatagg ggaacagtc agcaaagag gtggatttg ctgaaaaat gatattcag ataatgaaa agtcagttg gcacaaatt gaccaggag aaggacgag ttgctggtg gccttagat gacaagact gaaaagctc gcaatgatg gagcacgag ctgaaatcc cgcgaaaag acaatttct tcactcgaa tcggctgtg aatgaaatg aagagaaaa ctggggtaa
- >TC031105 RA
tctgacatg tggcgacac tttcaaatt ttgaagcgt ttgatgttt ttctaggtt atgtatttt tttaagttg taaatttaa ttttaaagt attttaacg atgtgagtt tgtgtttta atattttac aaaaatgga gatgaaagc cgcaaagct aagggaaga attagacga gctgggtta ctacctaac attttccca cgaatccgt cccccttgt cgaaaaact cgtcgccga cctgaaaac aacgaccca aagcctaca aaaatacat gaaaatagc ccaaaatgc aatcgagga acgagacaa tcttcagtt aggtgcgga gccctatcg gtgcgataa tgcgcggtt ggtgaaaga atgtaacga gttgcatca gcagttttt acgtgaaag ggaggaaaa tgagaaaat tcaaagaga tttaaagag gcgtttgga gtcgttgaa acaagaaaa cattgactg tcttaccga acgggagaa gttgaaagc taaaatcaa acaattgga agtggaaat tgtgaattg tggggaaat tctcttaaa tcccaaaca aaaacccaa acctgggct aaaaagtgc ctcaactac tgcgttagt tgggcagaa gtcgaccag aaaagcgaa aaattttga gctaaacag cgccatggc tttggcgga ccaaaaaat cgccaactt aagtaaaga tgtaaagaa gttgaagga gcataactt gcaattaat tgaagctaa tgaggtttt gcaatcaca gttaaataa tagagataa ggaaataaa gcgcttaag tggccttct ggaaggtgg aaggcccat tcaagcaat aaaaaaaga ttgtacgtg taattgcac ttgcactaa taaagattt acatgaggg tggttcaga tcaactgaa caaggtttt gcaagaaaa acacgttct ggaaaatcg tcttaaaga cgctctcgc taaacaaca cgaagcaat gaaacgttg catgcattt agcggaaag aaacaaatt gttggagaa agaaatgaa agacattga ccatttagc tttagctgt cgaagctga gtgtaacaa taccgttaa aaacaacgc ggagaaagt taatcgttt gcaggacag gatgcaaga aagtcttat tcaaattca aactttaga aagagaaaa caatcagct taaacaaga cacaaaaga actgagtga aaagttgga cgctattac gggagagaa aaaacattt acaaacggt tttagaaac gtccttgga ggaaaaaaa gcgactgac ggataaaat taacaactt cacgataat tgaacatga tttgaatat ggaaattga cagattgaa tcgcctttc agccgaaca aaagaaaaa aatcgccga attggaatc attagtgga ttcaaaagc acacacaga ggaggccaa gcgtatcac ttctgacgt gtccccaag tcggggtaa acccaaaat taagaaaaa acctccgaa aaaaccatc gaaaagtgg ttctcccgt tcaaccatc acttcaagt gcccacaaa tacgcccat tgatgggtc tcctgggac aggcaggtg ttgcaattg tcactgcga ttcctgttc ttcgtcaaa acatctcaa agagttgtt ggacaaaga actggaata tagggaaca acaagctgc tcgctgtat tgataattt gaagtcaga gaaagatta ttatatgag agaatatca taagatttt agaacaaat gaaaaatgt gcctagtcg gccgagtag tagtagaat tgataagaa taatgaact tgttcaaaa attgtcgga acaagaaca gacattagc tctcttaga acacaaaaa taggatgtt gtcgaaaga aaagtttga tttgatttc aaaacttga tagtgtgag ggatttacc tgatacaag tacggacgg tccttgtct caaagccaa ttgtaagcg caccgagcg tgagcgcga tcttttgcg ttcagatct tgaaagaat tgaagaaga acgagatat tctcagatc caaattaaa aaatgtgtc agaaggtca aattaacga acaagagag actgaaaaa acgtctctt agatgcgga agaccaaat tcacaaatt agaacagga acgtcgtga attgattca aaatcaagg aacaagaag atcggcaat tgatactct tgaagaaca atgcgagac tcttaaaaa tcaattacg cacagcaca aaacgaact gaatcaaca gagggcgtt atacaatca attaaagac tttgcatga acaaactga tcgctcatt atcagacac tcagagtaa attaattca agtggaatc ggaactcgc tagtgcctt agaaaattt gaaacggcg ggagcaaga taggggaac agtcagcaa agaggtgga tttgctgaa aaatgatat tcagataat gaaaagtca gttggcaca aattgacca ggagaagga cgagttgct ggtggcctt agatgacaa gactgaaaa gctcgcaat gatggagca cgagctgaa atcccgcga aaagacaat ttcttcact cgaatcggc tgtgaatga aatgaagag aaaactggg ctcgtccgt cgacgagaa tgcgaatca aagacatga aatacaaag tttagaaac ggaattagc gaccatgaa acaggaact tgacactat taaaaaaat taaagacag tgcgattca ggagaatcg cagacttca ggacgattt aggttcggt cacttgtga ctgtcggga cgctcgcaa ggagttaga attgtacaa gagacaagt ggatgattt gaaaactca gctccagca ttacgttgc tgaagttaa aagaaccga ggatctgat ctcgaataa agagattga aaggaacga gttgttgga tcagtttag gtcgctcag tcatgaagc caatatttt agaaactaa caatcacac tttagaaac cgaagctac gcaaagtag agtacaatt gtcggtcgc gttggatca tgccgccga tttggaacg caaactgga caataagga ctcgatcat tcacagtta tgagaaaca aattgctga tttgaccag tcaaattgc aagtttgga gatccagct cagacagtg catgaatca ttacgacaa agctgacga agagttgca aaacatgcg ggatttttc cttgaaatt agagtcgga aaatgcgcg tttgaagcg acagttgcg cgagaggga cgatcaaag aatgcaaat tgatcgaaa cgttgatca gttgagaaa cgaacgcga atttttaca acacacagt cacgcgaga cagacgcaa tgtggaatc ggttgaaag ggatttgca cgatgcgaa gcacgaaac caccgagtt gagaatctt gaatcggga tcttcagga agaagtgca aaatttgaa aatgcaagt gcaagaact aaaggaaaa atttgttgc aacgtccga acagttaga catgtacca agaaaaggc gctagagta cgcccaaca aaacaaaca gttaaggcg agaggtcgc aaatgagcg ctttcgcag gactagaga tagcgaaag ttccaaata tccgtccct ttaatcctt tttcccttt ccttaggca acaatacgt ccttgatca tgccacacc acaatccca gtcgccccc tgccagcct taaagtcct tatcagcac cagggaatt ccagtcaag ggccaactc ccacaaccc agatgacga gaggagact tcagggtga aaaagatca agctgtggc gtccgtcaa acaatgtct ttgcacgtg ttgttgtga aattaagag taagtctga agatgaagg tgttaaacg ttgctgttt ttccaatta tacaatggt acgtgaaac gcgagcgag caaagcgtc cacttcgaa aaaataaaa ttttagcac agtg
- >TC031105 RB
tctgacatg tggcgacac tttcaaatt ttgaagcgt ttgatgttt ttctaggtt atgtatttt tttaagttg taaatttaa ttttaaagt attttaacg atgtgagtt tgtgtttta atattttac aaaaatgga gatgaaagc cgcaaagct aagggaaga attagacga gctgggtta ctacctaac attttccca cgaatccgt cccccttgt cgaaaaact cgtcgccga cctgaaaac aacgaccca aagcctaca aaaatacat gaaaatagc ccaaaatgc aatcgagga acgagacaa tcttcagtt aggtgcgga gccctatcg gtgcgataa tgcgcggtt ggtgaaaga atgtaacga gttgcatca gcagttttt acgtgaaag ggaggaaaa tgagaaaat tcaaagaga tttaaagag gcgtttgga gtcgttgaa acaagaaaa cattgactg tcttaccga acgggagaa gttgaaagc taaaatcaa acaattgga agtggaaat tgtgaattg tggggaaat tctcttaaa tcccaaaca aaaacccaa acctgggct aaaaagtgc ctcaactac tgcgttagt tgggcagaa gtcgaccag aaaagcgaa aaattttga gctaaacag cgccatggc tttggcgga ccaaaaaat cgccaactt aagtaaaga tgtaaagaa gttgaagga gcataactt gcaattaat tgaagctaa tgaggtttt gcaatcaca gttaaataa tagagataa ggaaataaa gcgcttaag tggccttct ggaaggtgg aaggcccat tcaagcaat aaaaaaaga ttgtacgtg taattgcac ttgcactaa taaagattt acatgaggg tggttcaga tcaactgaa caaggtttt gcaagaaaa acacgttct ggaaaatcg tcttaaaga cgctctcgc taaacaaca cgaagcaat gaaacgttg catgcattt agcggaaag aaacaaatt gttggagaa agaaatgaa agacattga ccatttagc tttagctgt cgaagctga gtgtaacaa taccgttaa aaacaacgc ggagaaagt taatcgttt gcaggacag gatgcaaga aagtcttat tcaaattca aactttaga aagagaaaa caatcagct taaacaaga cacaaaaga actgagtga aaagttgga cgctattac gggagagaa aaaacattt acaaacggt tttagaaac gtccttgga ggaaaaaaa gcgactgac ggataaaat taacaactt cacgataat tgaacatga tttgaatat ggaaattga cagattgaa tcgcctttc agccgaaca aaagaaaaa aatcgccga attggaatc attagtgga ttcaaaagc acacacaga ggaggccaa gcgtatcac ttctgacgt gtccccaag tcggggtaa acccaaaat taagaaaaa acctccgaa aaaaccatc gaaaagtgg ttctcccgt tcaaccatc acttcaagt gcccacaaa tacgcccat tgatgggtc tcctgggac aggcaggtg ttgcaattg tcactgcga ttcctgttc ttcgtcaaa acatctcaa agagttgtt ggacaaaga actggaata tagggaaca acaagctgc tcgctgtat tgataattt gaagtcaga gaaagatta ttatatgag agaatatca taagatttt agaacaaat gaaaaatgt gcctagtcg gccgagtag tagtagaat tgataagaa taatgaact tgttcaaaa attgtcgga acaagaaca gacattagc tctcttaga acacaaaaa taggatgtt gtcgaaaga aaagtttga tttgatttc aaaacttga tagtgtgag ggatttacc tgatacaag tacggacgg tccttgtct caaagccaa ttgtaagcg caccgagcg tgagcgcga tcttttgcg ttcagatct tgaaagaat tgaagaaga acgagatat tctcagatc caaattaaa aaatgtgtc agaaggtca aattaacga acaagagag actgaaaaa acgtctctt agatgcgga agaccaaat tcacaaatt agaacagga acgtcgtga attgattca aaatcaagg aacaagaag atcggcaat tgatactct tgaagaaca atgcgagac tcttaaaaa tcaattacg cacagcaca aaacgaact gaatcaaca gagggcgtt atacaatca attaaagac tttgcatga acaaactga tcgctcatt atcagacac tcagagtaa attaattca agtggaatc ggaactcgc tagtgcctt agaaaattt gaaacggcg ggagcaaga taggggaac agtcagcaa agaggtgga tttgctgaa aaatgatat tcagataat gaaaagtca gttggcaca aattgacca ggagaagga cgagttgct ggtggcctt agatgacaa gactgaaaa gctcgcaat gatggagca cgagctgaa atcccgcga aaagacaat ttcttcact cgaatcggc tgtgaatga aatgaagag aaaactggg gtaagtttg aatttttat tacaaaagt aaaaatcaa tcgtataaa ttacaaagt t
