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Gene information
General information
TC008172 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6057778..6061372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008172
    iBeetle screen iB_01313

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    tropharium mostlyshortened - Number of animals showing the phenotype: 3
    vitellarium mostlydecreased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008172 RA
      MPPKKNKKS GKNGYWEFV LDCRNKHPN KQNMHEVQE 
YAARKWASM SKEERRPYE ERALLAREM YSPARYTTD 
GIDIEVVER KERDEARKK QEMKDDITR TLKAAYFAT 
DLDEKIFLV IHINHLAYY PTEDKYFIC EIAIAAVSL 
KNGVEDVFH RIVKPGKLP LGYYGGALT HSKETHQML 
ELVQDEPYE NNTREVFNE MTSFLKLWR GKGSDSIVY 
ADEKTHEMI TKVIDNFCQ EFNYPDEIK VYNFQYLFF 
ALRNSVAAR TVWPTETYS STELEKDLY SYTPDISCE 
FHEMSDISV YCSKSIVTR YCYTLCDHC CTDLNIQLV 
AGFHVPKNS RIAVDSSRT NSKAPSVCS TDDYRQAAR 
SRVGSFSST YDENYPALG SSRSSASSV VSFSSMRTP 
RNVGQPTKA ATRIEQSFK AMSVRDHRN LNFPPKGYE 
KQNEK
    CDS sequence
    • >TC008172 RA
      atgcctcct aagaagaat aagaaatcc ggcaaaaac 
ggctattgg gagtttgtt ttggattgt cggaataaa 
catccaaat aagcaaaat atgcacgaa gtacaagaa 
tacgctgca aggaaatgg gcgtccatg tcaaaggaa 
gaacgccgc ccgtatgaa gaacgtgcc ctactagcc 
cgagaaatg tactctcct gccaggtac acaactgat 
ggtattgac attgaagtg gttgagaga aaagagcga 
gatgaagca aggaagaaa caagaaatg aaagatgac 
attacgcga acactgaaa gcggcttat ttcgccact 
gatcttgac gaaaaaata ttcttagtt attcatatc 
aatcatctg gcgtattac cctactgag gataaatat 
tttatttgt gaaatagcc atagctgct gtaagtctt 
aaaaacgga gtggaggat gtgtttcat agaattgtc 
aaacccgga aaacttccg ttggggtat tatggagga 
gcattaact cattcgaag gaaactcac caaatgctt 
gagttggtt caggatgaa ccttatgag aacaatact 
cgtgaagtt tttaatgaa atgacttct ttcctgaaa 
ctttggaga ggcaaggga agtgattct atcgtttat 
gcggatgag aaaactcat gaaatgata acaaaagtc 
attgataat ttttgccaa gaattcaat tatcctgac 
gaaattaaa gtctacaac ttccaatat ctgtttttt 
gctttgaga aactcagta gctgcgcga accgtctgg 
cctacagaa acctacagt tctaccgaa cttgaaaaa 
gacctctac agctacaca cctgatatt tcttgcgaa 
tttcatgaa atgtcggac atttccgtc tactgtagc 
aagtcaatc gtgaccaga tactgctac acactttgt 
gaccattgc tgtactgac ttaaacatt caactagtg 
gctgggttt cacgtcccg aaaaattcc agaattgcg 
gtagattcg agcaggact aattccaaa gccccctca 
gtctgcagc actgatgat tatcgtcaa gccgcgaga 
agccgagtt ggttcattc agttcaaca tacgatgag 
aattatcca gctttgggg agcagcaga tcaagcgct 
tcttcggtt gtaagtttc tcctcaatg aggactccc 
cgaaatgtg ggacaaccg accaaagct gccaccaga 
attgaacaa tccttcaaa gccatgtct gtgagagat 
caccgcaat ctgaatttc cccccgaaa ggctacgaa 
aaacaaaat gaaaaataa
    mRNA sequence
    • >TC008172 RA
      tgggatttt tatcgaatt gggaactaa aaaggtttt 
tggggtttt accctttca gggaaattt agattttat 
gtgtggctg ttggtattt tctggtact tgtacttgc 
attctgaaa aaaaaccgt caatttttg ggggttaag 
cagtaagca gatgttttt tgcaattgc ttatctctt 
atcataaaa ttaaaccgc aactaccgc agatttgta 
ttatttaca tctgaagaa gtgcttagt tcttgttaa 
aatgcctcc taagaagaa taagaaatc cggcaaaaa 
cggctattg ggagtttgt tttggattg tcggaataa 
acatccaaa taagcaaaa tatgcacga agtacaaga 
atacgctgc aaggaaatg ggcgtccat gtcaaagga 
agaacgccg cccgtatga agaacgtgc cctactagc 
ccgagaaat gtactctcc tgccaggta cacaactga 
tggtattga cattgaagt ggttgagag aaaagagcg 
agatgaagc aaggaagaa acaagaaat gaaagatga 
cattacgcg aacactgaa agcggctta tttcgccac 
tgatcttga cgaaaaaat attcttagt tattcatat 
caatcatct ggcgtatta ccctactga ggataaata 
ttttatttg tgaaatagc catagctgc tgtaagtct 
taaaaacgg agtggagga tgtgtttca tagaattgt 
caaacccgg aaaacttcc gttggggta ttatggagg 
agcattaac tcattcgaa ggaaactca ccaaatgct 
tgagttggt tcaggatga accttatga gaacaatac 
tcgtgaagt ttttaatga aatgacttc tttcctgaa 
actttggag aggcaaggg aagtgattc tatcgttta 
tgcggatga gaaaactca tgaaatgat aacaaaagt 
cattgataa tttttgcca agaattcaa ttatcctga 
cgaaattaa agtctacaa cttccaata tctgttttt 
tgctttgag aaactcagt agctgcgcg aaccgtctg 
gcctacaga aacctacag ttctaccga acttgaaaa 
agacctcta cagctacac acctgatat ttcttgcga 
atttcatga aatgtcgga catttccgt ctactgtag 
caagtcaat cgtgaccag atactgcta cacactttg 
tgaccattg ctgtactga cttaaacat tcaactagt 
ggctgggtt tcacgtccc gaaaaattc cagaattgc 
ggtagattc gagcaggac taattccaa agccccctc 
agtctgcag cactgatga ttatcgtca agccgcgag 
aagccgagt tggttcatt cagttcaac atacgatga 
gaattatcc agctttggg gagcagcag atcaagcgc 
ttcttcggt tgtaagttt ctcctcaat gaggactcc 
ccgaaatgt gggacaacc gaccaaagc tgccaccag 
aattgaaca atccttcaa agccatgtc tgtgagaga 
tcaccgcaa tctgaattt ccccccgaa aggctacga 
aaaacaaaa tgaaaaata aaacgacac gcaagcaag 
caagcttgt aacgcattg ctcttatct aatgataat 
atgttaatt ttttacttg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY