| General information | |||
| TC008121 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5287785..5290159 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008121 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01311 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
- >TC008121 RA
MTNEQGVTV KQEVTDEED ESGMDVDDD VPTLGPAAL GLQRVGAPA APKPPPTQP TQVLNARGM PARIRKKNK LFYDDDIVN TPHHRVPSA KKMKQTPVK KPKVNTPTK SFKETPKRN TPRDVKSPR NISPPPLQS PDRKIGQKI GLRLRNVLK LPKAHKWVC YEWFYSDID RCLFSGEND FSICLKESF PELKTRELT RVQWTKIRR MMGKPRRCS QAFFHEERL ELEKKRKKI RALQQRKAT ELSSFKDLP PEIPMQLVI GTKVTARLR KPQDGLFTG SIDAVDTSN NTYRITFER QGLGTHSVP DYEVLSNEP PETLSLSSF QNKFRPWNG ITTYAAKIS PYTNLKFKK DPLLSGSMI NKPIFPQEE GRIGGYPTK LLEKMVLIT KILSVKRTK ISELKGMNA KAEKFNSFH SEIPEEFQR NYACILIDL EKLNHDLQL YLDDVQALC QEIAPEPSV AAMLAPSHL REKCREEAA EIVQRQNSI ISADKDPVK NKSILELIT DLTALMLQV KSLADSDQN AYELQVLQG TMEQIKRKL TPSNQLVFQ NSVEIHMKH IQVGLGQNL DKKLAINGI SKL
- >TC008121 RA
atgacgaac gagcagggg gtcactgtc aagcaggag gtgacggac gaggaggac gagtccggg atggacgtc gacgatgat gtccccacg ttggggccc gcggctctg ggcctgcag agagtgggt gctccagct gcacctaaa ccgccaccc actcaacca acgcaagtc ctaaacgcc cgtggtatg cctgcaagg atcaggaag aaaaataaa ctgttttac gacgacgat atcgtcaac acgccacat cacagggta ccttctgcg aaaaaaatg aagcaaacg cctgtgaaa aaacccaaa gttaacact cccacgaaa agtttcaaa gaaacgccc aagagaaac acaccgagg gacgtcaag tcgcctcgg aatatttca ccaccgccc cttcaatcg cccgatcgg aaaatcggg cagaaaatc ggactcaga ttgagaaat gtgctcaag ttgcccaag gcacacaaa tgggtctgc tacgagtgg ttttacagc gatatcgac cggtgtttg ttttcgggg gaaaatgat tttagtatc tgtctaaag gagtcgttt cctgagtta aaaacacga gagctgacg agagtacaa tggaccaaa attcgaaga atgatgggg aaaccccga cgttgctct caagctttc tttcacgag gagagatta gaattggag aaaaaacgg aaaaaaatc agagcgtta caacagagg aaagccacc gaactttcg agttttaaa gacttgcca cctgaaatc cccatgcaa ttggttatc ggtacaaaa gtaacagcc aggcttaga aagccacaa gacggatta ttcaccgga agcattgac gctgtggac acctcaaac aacacctac agaataaca ttcgagcgc cagggatta ggcacacat tctgtgcct gattatgaa gttttatca aacgaacca cccgaaacc ctctcgttg tccagcttc cagaacaag ttcagaccc tggaacggg atcacaaca tacgcggcg aaaatctcc ccttacaca aacctaaaa ttcaagaaa gatccctta ctgtcgggt tcgatgatc aataagccc attttcccg caggaggag ggccggata gggggatac ccgaccaaa cttttggaa aaaatggtc ttaatcacg aaaatcctc agcgtcaag aggacgaaa atcagcgaa ctgaagggc atgaacgcc aaggcggag aaatttaat tcgtttcat tcggagatt cctgaagag tttcagcgg aattacgcc tgcattttg atcgatctt gagaagtta aatcacgat ttgcagctg tatctggat gatgttcag gcgctctgc caggaaatc gcccctgag ccgagcgtg gcggcgatg ctggcgcca agccatttg agggaaaag tgccgagaa gaggccgct gaaattgta caaagacag aattcgatc atttcggcc gacaaggat ccagttaag aataaatcg attctggaa ctcatcacc gatctcacg gctttgatg ttgcaagta aaaagtttg gccgattcg gatcaaaac gcatacgaa ttgcaagtg ctccaaggc acgatggaa caaatcaag aggaagttg acgccaagt aatcaacta gtgttccag aactccgtc gaaattcac atgaaacac attcaagtc ggtctcggg caaaattta gataaaaaa ctcgccata aatggaatt tccaaacta tga
- >TC008121 RA
aacttgacg tcaatatgg cggacgaac ttgaaagta agtaaaacg cgtctagtt tgataaatt ccggagaaa ttcgagtgt tttggttgt gaaaattgc cccagaatt aatctttgg gcctttgtc gagtgaatc agattcagg gttcagaaa cgggcgcag aatgacgaa cgagcaggg ggtcactgt caagcagga ggtgacgga cgaggagga cgagtccgg gatggacgt cgacgatga tgtccccac gttggggcc cgcggctct gggcctgca gagagtggg tgctccagc tgcacctaa accgccacc cactcaacc aacgcaagt cctaaacgc ccgtggtat gcctgcaag gatcaggaa gaaaaataa actgtttta cgacgacga tatcgtcaa cacgccaca tcacagggt accttctgc gaaaaaaat gaagcaaac gcctgtgaa aaaacccaa agttaacac tcccacgaa aagtttcaa agaaacgcc caagagaaa cacaccgag ggacgtcaa gtcgcctcg gaatatttc accaccgcc ccttcaatc gcccgatcg gaaaatcgg gcagaaaat cggactcag attgagaaa tgtgctcaa gttgcccaa ggcacacaa atgggtctg ctacgagtg gttttacag cgatatcga ccggtgttt gttttcggg ggaaaatga ttttagtat ctgtctaaa ggagtcgtt tcctgagtt aaaaacacg agagctgac gagagtaca atggaccaa aattcgaag aatgatggg gaaaccccg acgttgctc tcaagcttt ctttcacga ggagagatt agaattgga gaaaaaacg gaaaaaaat cagagcgtt acaacagag gaaagccac cgaactttc gagttttaa agacttgcc acctgaaat ccccatgca attggttat cggtacaaa agtaacagc caggcttag aaagccaca agacggatt attcaccgg aagcattga cgctgtgga cacctcaaa caacaccta cagaataac attcgagcg ccagggatt aggcacaca ttctgtgcc tgattatga agttttatc aaacgaacc acccgaaac cctctcgtt gtccagctt ccagaacaa gttcagacc ctggaacgg gatcacaac atacgcggc gaaaatctc cccttacac aaacctaaa attcaagaa agatccctt actgtcggg ttcgatgat caataagcc cattttccc gcaggagga gggccggat agggggata cccgaccaa acttttgga aaaaatggt cttaatcac gaaaatcct cagcgtcaa gaggacgaa aatcagcga actgaaggg catgaacgc caaggcgga gaaatttaa ttcgtttca ttcggagat tcctgaaga gtttcagcg gaattacgc ctgcatttt gatcgatct tgagaagtt aaatcacga tttgcagct gtatctgga tgatgttca ggcgctctg ccaggaaat cgcccctga gccgagcgt ggcggcgat gctggcgcc aagccattt gagggaaaa gtgccgaga agaggccgc tgaaattgt acaaagaca gaattcgat catttcggc cgacaagga tccagttaa gaataaatc gattctgga actcatcac cgatctcac ggctttgat gttgcaagt aaaaagttt ggccgattc ggatcaaaa cgcatacga attgcaagt gctccaagg cacgatgga acaaatcaa gaggaagtt gacgccaag taatcaact agtgttcca gaactccgt cgaaattca catgaaaca cattcaagt cggtctcgg gcaaaattt agataaaaa actcgccat aaatggaat ttccaaact atgatactt tttttcttt aaaaatgca ggtttttaa agcagaatg ctgtgcaat actataatg gccacaatt tcgatttac acgtctcgt tttatctta ctgtttacc gcgggtttt atttataat tactgtatt tttgtgggt atttcacag aatgtgtat attttactt gtgtaatta ataaaaatg tatatatga ggcaccata tgtaatttt agaactgtt caaattttt atcaaaatt gg
