Status
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Gene information
General information
TC008118 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5267185..5275364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008118
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01310

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008118 RA
      MHTRGLIFW LILSFYRSA AEDAATEKL KSRDNFKST 
      YSANDLLLS DSSINTCSI DDFACLTMA ANDRLGLEA 
      IKTLHQKLD DDANGNVDL SESDDFLRE ELQYDSGYE 
      RRQKAFHRN DDMHISVKE LWEAWLKSE VHNWTVEQT 
      TDWLVSSVD LPQYVPSFI SHKVTGANL PRLAANNVN 
      YLNHLGIKD PIHKQKIVL KAMDVVLFG PPKDGQPHW 
      KDLTLIVLL IGGGIGVWY AFQQNKKFK NHLNRMNRD 
      MDSLQNAEK ALENLQKEL EEAKQAQED VITEKQNLE 
      KKLQDSKTD LDSLPYSDL EVNQLKAEI EMLRTELQI 
      AEGELKDRC WSPPPGLQQ WLQLTHEIE NKAYMKKKA 
      SAEKQLQQA REACEKLRK KRSSLVGAF VSTHGKSID 
      EVDRSIVEA RTALNEVTQ ELQERVHRW KQIEMLCGF 
      SIINNNGLQ FLENTLYRN TNGRALPVR GRISSQDDL 
      DDDTASLYG HQGHSEGTA LHSWKEGDS SGSETSRQE 
      EDSHSESKP TPGVHFMVG GEISTWPED SSSYTKISK 
      LSSNPRSIS NNNIPLTNK SSQMTRSFS QDTNVGTLD 
      SVKPKSSLS DTSLDAPLK PKPLRQTTI KEQPSASIE 
      EDICSTDSS LMDEDIKKK KKKLFAFTK KSKGKND
    CDS sequence
    • >TC008118 RA
      atgcatacc cggggacta attttttgg ttaatcttg 
      agcttttac cgaagtgct gccgaagat gccgccacg 
      gaaaaactc aagtcgcgg gacaacttc aagagtact 
      tactcggcc aacgactta ctcctcagc gacagctcc 
      atcaacacc tgcagcatc gacgacttc gcctgcctc 
      acgatggcc gccaacgac cgcctgggg ctggaagcc 
      atcaagacc ctgcaccag aagctggac gacgacgcc 
      aacggcaac gtggacctc tcggaatcc gatgacttc 
      ctcagagag gaattgcaa tacgactct ggctacgag 
      cgccgacag aaggccttc catcgcaac gacgacatg 
      cacatctcc gtcaaggaa ctgtgggag gcctggttg 
      aagtcagaa gtgcacaac tggacggtt gaacaaacc 
      accgattgg ctcgtctct tcggttgat ttgccccaa 
      tatgttcct agttttatc tcgcacaaa gtcaccggg 
      gctaactta cccagattg gctgccaat aacgttaac 
      tatcttaac catctcgga atcaaggac ccgatacat 
      aagcaaaaa atcgtcctc aaggccatg gacgtggtg 
      ctttttggc cccccaaag gacggccag ccgcattgg 
      aaggacctc accttgatc gtccttctc atcggtggt 
      ggaatcggt gtttggtat gcgttccaa caaaacaaa 
      aaattcaaa aaccacctg aatcgcatg aaccgtgac 
      atggacagt ttacagaac gcggaaaag gcgttagaa 
      aacttacaa aaagaactc gaagaagcc aaacaagcg 
      caagaagac gtaatcacc gaaaaacag aatttagaa 
      aagaaattg caagattcg aaaacggat ctcgactca 
      ttgccgtat tccgatttg gaggtgaac caactcaag 
      gccgaaatc gagatgctg aggactgaa ctgcagatc 
      gctgagggg gagctgaag gacaggtgc tggtcgccg 
      ccgccgggc ctccaacag tggctgcag ctcacccac 
      gaaatagaa aataaagcc tacatgaag aaaaaagcg 
      tcggcggaa aaacaacta caacaagcg agagaagct 
      tgtgagaaa ttgaggaag aagcggtcg agtctcgtc 
      ggtgctttt gtctcaact cacggcaaa tccatcgat 
      gaagttgat aggagcata gttgaggcg cggacggcc 
      ctgaatgag gtcacccaa gaattgcaa gagcgtgtc 
      catagatgg aagcagatt gagatgttg tgtggcttc 
      agcatcatc aacaataac ggtttgcag tttttggaa 
      aacaccctg tatcgtaat actaacgga cgggcgctg 
      ccagtacga ggtcgcatc agcagtcag gatgatctg 
      gacgacgat acagcttcg ctctacggc caccaaggc 
      cattcagag gggaccgcg ctgcattcg tggaaagaa 
      ggtgactct tcagggagc gaaactagc cgacaggag 
      gaagactcc cattcggag agcaagccg acgcctgga 
      gtccacttc atggtggga ggagagatc tcaacttgg 
      cccgaagat tcaagtagt tacactaaa attagcaaa 
      ttgtcttcc aatccgcga agtattagc aacaataat 
      atcccgttg acgaacaaa tcgtcgcaa atgacccgc 
      tctttttcg caagatacg aacgtaggg actttggac 
      tcggtcaag ccgaagtcc tcgctgtcc gatacctcg 
      ttggacgcg cctcttaag cccaaaccg ctgcggcag 
      acgacaata aaagagcag ccgtcggcg tcaatcgag 
      gaggatatc tgttcgacg gactcctct ctaatggac 
      gaagatatc aagaagaag aagaaaaag ctgttcgcg 
      ttcacgaaa aagagcaag ggtaaaaat gactga
    mRNA sequence
    • >TC008118 RA
      gttccgaaa taaataaaa cgataacag tgaatagca 
      gaaacaagg tttaaaaat caacacttg gaaatgtac 
      tccaatttc ctctgaagg ttaaaaacg atgcatacc 
      cggggacta attttttgg ttaatcttg agcttttac 
      cgaagtgct gccgaagat gccgccacg gaaaaactc 
      aagtcgcgg gacaacttc aagagtact tactcggcc 
      aacgactta ctcctcagc gacagctcc atcaacacc 
      tgcagcatc gacgacttc gcctgcctc acgatggcc 
      gccaacgac cgcctgggg ctggaagcc atcaagacc 
      ctgcaccag aagctggac gacgacgcc aacggcaac 
      gtggacctc tcggaatcc gatgacttc ctcagagag 
      gaattgcaa tacgactct ggctacgag cgccgacag 
      aaggccttc catcgcaac gacgacatg cacatctcc 
      gtcaaggaa ctgtgggag gcctggttg aagtcagaa 
      gtgcacaac tggacggtt gaacaaacc accgattgg 
      ctcgtctct tcggttgat ttgccccaa tatgttcct 
      agttttatc tcgcacaaa gtcaccggg gctaactta 
      cccagattg gctgccaat aacgttaac tatcttaac 
      catctcgga atcaaggac ccgatacat aagcaaaaa 
      atcgtcctc aaggccatg gacgtggtg ctttttggc 
      cccccaaag gacggccag ccgcattgg aaggacctc 
      accttgatc gtccttctc atcggtggt ggaatcggt 
      gtttggtat gcgttccaa caaaacaaa aaattcaaa 
      aaccacctg aatcgcatg aaccgtgac atggacagt 
      ttacagaac gcggaaaag gcgttagaa aacttacaa 
      aaagaactc gaagaagcc aaacaagcg caagaagac 
      gtaatcacc gaaaaacag aatttagaa aagaaattg 
      caagattcg aaaacggat ctcgactca ttgccgtat 
      tccgatttg gaggtgaac caactcaag gccgaaatc 
      gagatgctg aggactgaa ctgcagatc gctgagggg 
      gagctgaag gacaggtgc tggtcgccg ccgccgggc 
      ctccaacag tggctgcag ctcacccac gaaatagaa 
      aataaagcc tacatgaag aaaaaagcg tcggcggaa 
      aaacaacta caacaagcg agagaagct tgtgagaaa 
      ttgaggaag aagcggtcg agtctcgtc ggtgctttt 
      gtctcaact cacggcaaa tccatcgat gaagttgat 
      aggagcata gttgaggcg cggacggcc ctgaatgag 
      gtcacccaa gaattgcaa gagcgtgtc catagatgg 
      aagcagatt gagatgttg tgtggcttc agcatcatc 
      aacaataac ggtttgcag tttttggaa aacaccctg 
      tatcgtaat actaacgga cgggcgctg ccagtacga 
      ggtcgcatc agcagtcag gatgatctg gacgacgat 
      acagcttcg ctctacggc caccaaggc cattcagag 
      gggaccgcg ctgcattcg tggaaagaa ggtgactct 
      tcagggagc gaaactagc cgacaggag gaagactcc 
      cattcggag agcaagccg acgcctgga gtccacttc 
      atggtggga ggagagatc tcaacttgg cccgaagat 
      tcaagtagt tacactaaa attagcaaa ttgtcttcc 
      aatccgcga agtattagc aacaataat atcccgttg 
      acgaacaaa tcgtcgcaa atgacccgc tctttttcg 
      caagatacg aacgtaggg actttggac tcggtcaag 
      ccgaagtcc tcgctgtcc gatacctcg ttggacgcg 
      cctcttaag cccaaaccg ctgcggcag acgacaata 
      aaagagcag ccgtcggcg tcaatcgag gaggatatc 
      tgttcgacg gactcctct ctaatggac gaagatatc 
      aagaagaag aagaaaaag ctgttcgcg ttcacgaaa 
      aagagcaag ggtaaaaat gactgaggg tgagtcggc 
      gtttaggat gacttgtct tgttatccc ggggatcgt 
      taaacaggg ataatgtac ctcgattat gtttcttta 
      attttgtag ctactaata ggtaatttt cgatatgtc 
      attttaggt ttgctagtt taatgcttc gacgtattg 
      aaaattcaa tttttccca aggaaagtt aactgaaaa 
      tgtgcagat attagtctt ccacttaac ccgagttaa 
      ttattttgt atattttgg acgaatcct aggaatgtt 
      tttgttcct taatttcca gttttattt attccattg 
      tacttagaa atcgtgact gaactgtcc taaagcgcc 
      taagtgtac ttcaattat tgtgatgat aattgtatc 
      tcttttatt ttccttctt aaccgatca ttgggcttt 
      gcagcaaag ttaattgtg ataaattag tagacgaga 
      attttattt tcgtgaatt ttatttaga ttaaaaata 
      aagacaggg gttttactc gtttagttt atttcacag 
      cttccttaa ttaatgatt ggaggtttc cttcttcat 
      tgacgaggg gagcggtag tttatttta taatattca 
      tttgcaata attgagtaa ttgtaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY