General information | |||
TC008107 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5040923..5043638 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008107 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01309 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)
- >TC008107 RA
MANLKPGGQ NPRKKVQVS MVICEAEEK RHRSGVNAL QIDPALDRL YSAGRDSII RVYEHERYL HGMEHHTDW VNDIVLCCG GRHLISASS DTTVKVWNA HKGFCMSTL RTHKDYVKA LAYAKDREQ VASAGLDKS IYLWDINTL TALTASNNT VTTSSLMGS KESIYSLAM NPPGTVIVS GSTEKALRL WDPRNCVKL FKLKGHTDN VKALVVSKD GTHCISGSS DGSIKVWSL GGQRCIQTI RVHSDSVWA LLATENFTH VISGGRDKK VIMTDLKNP QNSVVVCVE EAPVLKMCF TADQQAVWV STSNSSIRC WKLPTEKHF KEEAKMPTS PISLIPGGA AIRQAVVLN DKRHILTKD ANDNVALYD VLRARKIED LGQINFQDE IKNRFKVVY VPNWFNVDL KTGMLTIHL GQDEVDCFS AWVSARDAG IRDCPDPDQ KVNYGKLLL QALFEHWRG VERDPENKL YFSVPKHIP LILSEVGGR TLYRVLVGD TVGDTENAL LNETVPNWV ISIIEENST GKFIKVQFY LQPHSSVPS HLLKQDRLK KPDRLVAND FIQCRKVAE HILEKLLGD GNASSPGGD ADSTTPSGN GYNVDQIEL TCNDQVLDP AMDLRTVKH FIWKSSADL TLHYRIVNP K
- >TC008107 RB
MVICEAEEK RHRSGVNAL QIDPALDRL YSAGRDSII RVYEHERYL HGMEHHTDW VNDIVLCCG GRHLISASS DTTVKVWNA HKGFCMSTL RTHKDYVKA LAYAKDREQ VASAGLDKS IYLWDINTL TALTASNNT VTTSSLMGS KESIYSLAM NPPGTVIVS GSTEKALRL WDPRNCVKL FKLKGHTDN VKALVVSKD GTHCISGSS DGSIKVWSL GGQRCIQTI RVHSDSVWA LLATENFTH VISGGRDKK VIMTDLKNP QNSVVVCVE EAPVLKMCF TADQQAVWV STSNSSIRC WKLPTEKHF KEEAKMPTS PISLIPGGA AIRQAVVLN DKRHILTKD ANDNVALYD VLRARKIED LGQINFQDE IKNRFKVVY VPNWFNVDL KTGMLTIHL GQDEVDCFS AWVSARDAG IRDCPDPDQ KVNYGKLLL QALFEHWRG VERDPENKL YFSVPKHIP LILSEVGGR TLYRVLVGD TVGDTENAL LNETVPNWV ISIIEENST GKFIKVQFY LQPHSSVPS HLLKQDRLK KPDRLVAND FIQCRKVAE HILEKLLGD GNASSPGGD ADSTTPSGN GYNVDQIEL TCNDQVLDP AMDLRTVKH FIWKSSADL TLHYRIVNP K
- >TC008107 RA
atggcaaat ctgaagcca ggcgggcaa aatccgcga aaaaaagtc caggtatca atggtaatt tgcgaagct gaggagaaa agacacaga tctggggta aatgccctt cagatagat ccggcatta gaccgtttg tattctgct gggagggac tcaattatt agggtgtat gaacatgag cgctattta cacggaatg gagcaccat actgattgg gttaatgac atagttctg tgttgcgga ggccggcac ttaatctct gcaagttct gacactact gttaaagta tggaacgcc cacaagggc ttctgcatg tcaactctg cgaacacat aaagactat gtgaaagcc ttggcttac gccaaagac agagaacaa gtcgcgtct gcaggtctt gataaatcc atttacttg tgggacatc aacacctta acagcacta acagcgagc aataacaca gtcactact tctagtttg atgggcagc aaagaatca atttatagc ctagcaatg aaccctcca ggcactgtt atagtaagc ggatccacc gagaaagcc ctcaggctt tgggacccg cgaaattgc gtcaagttg tttaagtta aaaggtcac actgataac gttaaggct ttagttgtg tcaaaagat ggcactcat tgcatttct ggcagcagc gatgggtcc atcaaagtg tggagttta gggggccag aggtgtatc caaacaatc agagtgcat agcgactcc gtttgggca cttctggcc acggaaaat ttcactcat gtaatctcc ggtggtagg gacaagaag gtgataatg actgatctg aaaaacccc cagaacagc gttgtggtc tgtgtggag gaggcgcca gtgctcaaa atgtgcttc acggcagac cagcaggct gtctgggtg tcaacttcc aactccagc attcgctgt tggaagttg ccaaccgag aaacacttc aaagaagag gccaaaatg ccgacatcg cccatttcg ctgatacca ggcggtgct gcgatacgt caggccgtc gttctcaac gacaagaga cacattttg accaaagat gccaacgat aatgtcgct ctttacgac gttttgcgt gcgcgaaaa atcgaagac ctaggccaa atcaatttc caagacgaa ataaaaaac cgattcaaa gtagtttac gtcccgaat tggttcaat gttgattta aagactgga atgctgacg attcattta ggacaggac gaagtcgat tgcttctcg gcctgggtt tcggccagg gatgcggga atcagggac tgtccagac cccgaccaa aaagtaaac tacggaaaa ttgctactg caagcactg ttcgagcat tggagaggc gtggagaga gaccccgaa aataaactg tacttctca gtacccaaa catataccc ttgattttg agtgaagtg ggcgggcgc acgctttac cgggtcttg gtgggcgac acagtcgga gacaccgaa aatgcgctc ctgaacgaa acagtgcct aattgggtt atttccatt attgaggag aacagtacc gggaaattc atcaaagtg cagttttac cttcagccg cactcttcg gtcccgtct catttattg aaacaagac agactgaaa aaacccgat agattggta gccaatgat tttatccag tgtcgcaaa gttgccgaa catattttg gagaaatta ctgggggat ggaaacgca agttctccc ggaggtgac gccgattcg accactccc agcggcaac gggtacaac gttgatcaa attgaactg acctgcaat gaccaagtc ctggaccca gccatggac ttacgaacc gttaaacat ttcatctgg aaatcttcg gcagacctg acgctgcac tacaggatt gtcaacccc aaataa
- >TC008107 RB
atggtaatt tgcgaagct gaggagaaa agacacaga tctggggta aatgccctt cagatagat ccggcatta gaccgtttg tattctgct gggagggac tcaattatt agggtgtat gaacatgag cgctattta cacggaatg gagcaccat actgattgg gttaatgac atagttctg tgttgcgga ggccggcac ttaatctct gcaagttct gacactact gttaaagta tggaacgcc cacaagggc ttctgcatg tcaactctg cgaacacat aaagactat gtgaaagcc ttggcttac gccaaagac agagaacaa gtcgcgtct gcaggtctt gataaatcc atttacttg tgggacatc aacacctta acagcacta acagcgagc aataacaca gtcactact tctagtttg atgggcagc aaagaatca atttatagc ctagcaatg aaccctcca ggcactgtt atagtaagc ggatccacc gagaaagcc ctcaggctt tgggacccg cgaaattgc gtcaagttg tttaagtta aaaggtcac actgataac gttaaggct ttagttgtg tcaaaagat ggcactcat tgcatttct ggcagcagc gatgggtcc atcaaagtg tggagttta gggggccag aggtgtatc caaacaatc agagtgcat agcgactcc gtttgggca cttctggcc acggaaaat ttcactcat gtaatctcc ggtggtagg gacaagaag gtgataatg actgatctg aaaaacccc cagaacagc gttgtggtc tgtgtggag gaggcgcca gtgctcaaa atgtgcttc acggcagac cagcaggct gtctgggtg tcaacttcc aactccagc attcgctgt tggaagttg ccaaccgag aaacacttc aaagaagag gccaaaatg ccgacatcg cccatttcg ctgatacca ggcggtgct gcgatacgt caggccgtc gttctcaac gacaagaga cacattttg accaaagat gccaacgat aatgtcgct ctttacgac gttttgcgt gcgcgaaaa atcgaagac ctaggccaa atcaatttc caagacgaa ataaaaaac cgattcaaa gtagtttac gtcccgaat tggttcaat gttgattta aagactgga atgctgacg attcattta ggacaggac gaagtcgat tgcttctcg gcctgggtt tcggccagg gatgcggga atcagggac tgtccagac cccgaccaa aaagtaaac tacggaaaa ttgctactg caagcactg ttcgagcat tggagaggc gtggagaga gaccccgaa aataaactg tacttctca gtacccaaa catataccc ttgattttg agtgaagtg ggcgggcgc acgctttac cgggtcttg gtgggcgac acagtcgga gacaccgaa aatgcgctc ctgaacgaa acagtgcct aattgggtt atttccatt attgaggag aacagtacc gggaaattc atcaaagtg cagttttac cttcagccg cactcttcg gtcccgtct catttattg aaacaagac agactgaaa aaacccgat agattggta gccaatgat tttatccag tgtcgcaaa gttgccgaa catattttg gagaaatta ctgggggat ggaaacgca agttctccc ggaggtgac gccgattcg accactccc agcggcaac gggtacaac gttgatcaa attgaactg acctgcaat gaccaagtc ctggaccca gccatggac ttacgaacc gttaaacat ttcatctgg aaatcttcg gcagacctg acgctgcac tacaggatt gtcaacccc aaataa
- >TC008107 RA
tgtcaaaat aaagtcaga ccgttctaa cctcaagtt tttacaaag cttttcaaa aaagctttt tttacacta atcacgcca atttatagc ccatttctt taaataaaa aatgccaca ttgctaaac ttccaaaaa atggcaaat ctgaagcca ggcgggcaa aatccgcga aaaaaagtc caggtatca atggtaatt tgcgaagct gaggagaaa agacacaga tctggggta aatgccctt cagatagat ccggcatta gaccgtttg tattctgct gggagggac tcaattatt agggtgtat gaacatgag cgctattta cacggaatg gagcaccat actgattgg gttaatgac atagttctg tgttgcgga ggccggcac ttaatctct gcaagttct gacactact gttaaagta tggaacgcc cacaagggc ttctgcatg tcaactctg cgaacacat aaagactat gtgaaagcc ttggcttac gccaaagac agagaacaa gtcgcgtct gcaggtctt gataaatcc atttacttg tgggacatc aacacctta acagcacta acagcgagc aataacaca gtcactact tctagtttg atgggcagc aaagaatca atttatagc ctagcaatg aaccctcca ggcactgtt atagtaagc ggatccacc gagaaagcc ctcaggctt tgggacccg cgaaattgc gtcaagttg tttaagtta aaaggtcac actgataac gttaaggct ttagttgtg tcaaaagat ggcactcat tgcatttct ggcagcagc gatgggtcc atcaaagtg tggagttta gggggccag aggtgtatc caaacaatc agagtgcat agcgactcc gtttgggca cttctggcc acggaaaat ttcactcat gtaatctcc ggtggtagg gacaagaag gtgataatg actgatctg aaaaacccc cagaacagc gttgtggtc tgtgtggag gaggcgcca gtgctcaaa atgtgcttc acggcagac cagcaggct gtctgggtg tcaacttcc aactccagc attcgctgt tggaagttg ccaaccgag aaacacttc aaagaagag gccaaaatg ccgacatcg cccatttcg ctgatacca ggcggtgct gcgatacgt caggccgtc gttctcaac gacaagaga cacattttg accaaagat gccaacgat aatgtcgct ctttacgac gttttgcgt gcgcgaaaa atcgaagac ctaggccaa atcaatttc caagacgaa ataaaaaac cgattcaaa gtagtttac gtcccgaat tggttcaat gttgattta aagactgga atgctgacg attcattta ggacaggac gaagtcgat tgcttctcg gcctgggtt tcggccagg gatgcggga atcagggac tgtccagac cccgaccaa aaagtaaac tacggaaaa ttgctactg caagcactg ttcgagcat tggagaggc gtggagaga gaccccgaa aataaactg tacttctca gtacccaaa catataccc ttgattttg agtgaagtg ggcgggcgc acgctttac cgggtcttg gtgggcgac acagtcgga gacaccgaa aatgcgctc ctgaacgaa acagtgcct aattgggtt atttccatt attgaggag aacagtacc gggaaattc atcaaagtg cagttttac cttcagccg cactcttcg gtcccgtct catttattg aaacaagac agactgaaa aaacccgat agattggta gccaatgat tttatccag tgtcgcaaa gttgccgaa catattttg gagaaatta ctgggggat ggaaacgca agttctccc ggaggtgac gccgattcg accactccc agcggcaac gggtacaac gttgatcaa attgaactg acctgcaat gaccaagtc ctggaccca gccatggac ttacgaacc gttaaacat ttcatctgg aaatcttcg gcagacctg acgctgcac tacaggatt gtcaacccc aaataattc attccttac acttattta tttatccat gattttggt ggacagatt tatactagt ttcttgtaa tcaaacatt tagcagtat atgtttttc gtatttgtt tgcaataaa aatatgtaa cacga
- >TC008107 RB
tgtcaaaat aaagtcaga ccgttctaa cctcaagtt tttacaaag cttttcaaa aaagctttt tttacacta atcacgcca atttatagc ccatttctt taaataaaa aatgccaca ttgctaaac ttccaaaaa atggcaaat ctgaagcca ggcgggcaa aatccgcga aaaaaagtc caggttagt tgtttacat tctgtattt acaaaacag ttttttgca acaatcttg gccaggtat caatggtaa tttgcgaag ctgaggaga aaagacaca gatctgggg taaatgccc ttcagatag atccggcat tagaccgtt tgtattctg ctgggaggg actcaatta ttagggtgt atgaacatg agcgctatt tacacggaa tggagcacc atactgatt gggttaatg acatagttc tgtgttgcg gaggccggc acttaatct ctgcaagtt ctgacacta ctgttaaag tatggaacg cccacaagg gcttctgca tgtcaactc tgcgaacac ataaagact atgtgaaag ccttggctt acgccaaag acagagaac aagtcgcgt ctgcaggtc ttgataaat ccatttact tgtgggaca tcaacacct taacagcac taacagcga gcaataaca cagtcacta cttctagtt tgatgggca gcaaagaat caatttata gcctagcaa tgaaccctc caggcactg ttatagtaa gcggatcca ccgagaaag ccctcaggc tttgggacc cgcgaaatt gcgtcaagt tgtttaagt taaaaggtc acactgata acgttaagg ctttagttg tgtcaaaag atggcactc attgcattt ctggcagca gcgatgggt ccatcaaag tgtggagtt tagggggcc agaggtgta tccaaacaa tcagagtgc atagcgact ccgtttggg cacttctgg ccacggaaa atttcactc atgtaatct ccggtggta gggacaaga aggtgataa tgactgatc tgaaaaacc cccagaaca gcgttgtgg tctgtgtgg aggaggcgc cagtgctca aaatgtgct tcacggcag accagcagg ctgtctggg tgtcaactt ccaactcca gcattcgct gttggaagt tgccaaccg agaaacact tcaaagaag aggccaaaa tgccgacat cgcccattt cgctgatac caggcggtg ctgcgatac gtcaggccg tcgttctca acgacaaga gacacattt tgaccaaag atgccaacg ataatgtcg ctctttacg acgttttgc gtgcgcgaa aaatcgaag acctaggcc aaatcaatt tccaagacg aaataaaaa accgattca aagtagttt acgtcccga attggttca atgttgatt taaagactg gaatgctga cgattcatt taggacagg acgaagtcg attgcttct cggcctggg tttcggcca gggatgcgg gaatcaggg actgtccag accccgacc aaaaagtaa actacggaa aattgctac tgcaagcac tgttcgagc attggagag gcgtggaga gagaccccg aaaataaac tgtacttct cagtaccca aacatatac ccttgattt tgagtgaag tgggcgggc gcacgcttt accgggtct tggtgggcg acacagtcg gagacaccg aaaatgcgc tcctgaacg aaacagtgc ctaattggg ttatttcca ttattgagg agaacagta ccgggaaat tcatcaaag tgcagtttt accttcagc cgcactctt cggtcccgt ctcatttat tgaaacaag acagactga aaaaacccg atagattgg tagccaatg attttatcc agtgtcgca aagttgccg aacatattt tggagaaat tactggggg atggaaacg caagttctc ccggaggtg acgccgatt cgaccactc ccagcggca acgggtaca acgttgatc aaattgaac tgacctgca atgaccaag tcctggacc cagccatgg acttacgaa ccgttaaac atttcatct ggaaatctt cggcagacc tgacgctgc actacagga ttgtcaacc ccaaataat tcattcctt acacttatt tatttatcc atgattttg gtggacaga tttatacta gtttcttgt aatcaaaca tttagcagt atatgtttt tcgtatttg tttgcaata aaaatatgt aacacga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
