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Gene information
General information
TC008107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 5040923..5043638
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008107
    iBeetle screen iB_01309
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008107 RA
      MANLKPGGQ NPRKKVQVS MVICEAEEK RHRSGVNAL 
      QIDPALDRL YSAGRDSII RVYEHERYL HGMEHHTDW 
      VNDIVLCCG GRHLISASS DTTVKVWNA HKGFCMSTL 
      RTHKDYVKA LAYAKDREQ VASAGLDKS IYLWDINTL 
      TALTASNNT VTTSSLMGS KESIYSLAM NPPGTVIVS 
      GSTEKALRL WDPRNCVKL FKLKGHTDN VKALVVSKD 
      GTHCISGSS DGSIKVWSL GGQRCIQTI RVHSDSVWA 
      LLATENFTH VISGGRDKK VIMTDLKNP QNSVVVCVE 
      EAPVLKMCF TADQQAVWV STSNSSIRC WKLPTEKHF 
      KEEAKMPTS PISLIPGGA AIRQAVVLN DKRHILTKD 
      ANDNVALYD VLRARKIED LGQINFQDE IKNRFKVVY 
      VPNWFNVDL KTGMLTIHL GQDEVDCFS AWVSARDAG 
      IRDCPDPDQ KVNYGKLLL QALFEHWRG VERDPENKL 
      YFSVPKHIP LILSEVGGR TLYRVLVGD TVGDTENAL 
      LNETVPNWV ISIIEENST GKFIKVQFY LQPHSSVPS 
      HLLKQDRLK KPDRLVAND FIQCRKVAE HILEKLLGD 
      GNASSPGGD ADSTTPSGN GYNVDQIEL TCNDQVLDP 
      AMDLRTVKH FIWKSSADL TLHYRIVNP K
    • >TC008107 RB
      MVICEAEEK RHRSGVNAL QIDPALDRL YSAGRDSII 
      RVYEHERYL HGMEHHTDW VNDIVLCCG GRHLISASS 
      DTTVKVWNA HKGFCMSTL RTHKDYVKA LAYAKDREQ 
      VASAGLDKS IYLWDINTL TALTASNNT VTTSSLMGS 
      KESIYSLAM NPPGTVIVS GSTEKALRL WDPRNCVKL 
      FKLKGHTDN VKALVVSKD GTHCISGSS DGSIKVWSL 
      GGQRCIQTI RVHSDSVWA LLATENFTH VISGGRDKK 
      VIMTDLKNP QNSVVVCVE EAPVLKMCF TADQQAVWV 
      STSNSSIRC WKLPTEKHF KEEAKMPTS PISLIPGGA 
      AIRQAVVLN DKRHILTKD ANDNVALYD VLRARKIED 
      LGQINFQDE IKNRFKVVY VPNWFNVDL KTGMLTIHL 
      GQDEVDCFS AWVSARDAG IRDCPDPDQ KVNYGKLLL 
      QALFEHWRG VERDPENKL YFSVPKHIP LILSEVGGR 
      TLYRVLVGD TVGDTENAL LNETVPNWV ISIIEENST 
      GKFIKVQFY LQPHSSVPS HLLKQDRLK KPDRLVAND 
      FIQCRKVAE HILEKLLGD GNASSPGGD ADSTTPSGN 
      GYNVDQIEL TCNDQVLDP AMDLRTVKH FIWKSSADL 
      TLHYRIVNP K
    CDS sequence
    • >TC008107 RA
      atggcaaat ctgaagcca ggcgggcaa aatccgcga 
      aaaaaagtc caggtatca atggtaatt tgcgaagct 
      gaggagaaa agacacaga tctggggta aatgccctt 
      cagatagat ccggcatta gaccgtttg tattctgct 
      gggagggac tcaattatt agggtgtat gaacatgag 
      cgctattta cacggaatg gagcaccat actgattgg 
      gttaatgac atagttctg tgttgcgga ggccggcac 
      ttaatctct gcaagttct gacactact gttaaagta 
      tggaacgcc cacaagggc ttctgcatg tcaactctg 
      cgaacacat aaagactat gtgaaagcc ttggcttac 
      gccaaagac agagaacaa gtcgcgtct gcaggtctt 
      gataaatcc atttacttg tgggacatc aacacctta 
      acagcacta acagcgagc aataacaca gtcactact 
      tctagtttg atgggcagc aaagaatca atttatagc 
      ctagcaatg aaccctcca ggcactgtt atagtaagc 
      ggatccacc gagaaagcc ctcaggctt tgggacccg 
      cgaaattgc gtcaagttg tttaagtta aaaggtcac 
      actgataac gttaaggct ttagttgtg tcaaaagat 
      ggcactcat tgcatttct ggcagcagc gatgggtcc 
      atcaaagtg tggagttta gggggccag aggtgtatc 
      caaacaatc agagtgcat agcgactcc gtttgggca 
      cttctggcc acggaaaat ttcactcat gtaatctcc 
      ggtggtagg gacaagaag gtgataatg actgatctg 
      aaaaacccc cagaacagc gttgtggtc tgtgtggag 
      gaggcgcca gtgctcaaa atgtgcttc acggcagac 
      cagcaggct gtctgggtg tcaacttcc aactccagc 
      attcgctgt tggaagttg ccaaccgag aaacacttc 
      aaagaagag gccaaaatg ccgacatcg cccatttcg 
      ctgatacca ggcggtgct gcgatacgt caggccgtc 
      gttctcaac gacaagaga cacattttg accaaagat 
      gccaacgat aatgtcgct ctttacgac gttttgcgt 
      gcgcgaaaa atcgaagac ctaggccaa atcaatttc 
      caagacgaa ataaaaaac cgattcaaa gtagtttac 
      gtcccgaat tggttcaat gttgattta aagactgga 
      atgctgacg attcattta ggacaggac gaagtcgat 
      tgcttctcg gcctgggtt tcggccagg gatgcggga 
      atcagggac tgtccagac cccgaccaa aaagtaaac 
      tacggaaaa ttgctactg caagcactg ttcgagcat 
      tggagaggc gtggagaga gaccccgaa aataaactg 
      tacttctca gtacccaaa catataccc ttgattttg 
      agtgaagtg ggcgggcgc acgctttac cgggtcttg 
      gtgggcgac acagtcgga gacaccgaa aatgcgctc 
      ctgaacgaa acagtgcct aattgggtt atttccatt 
      attgaggag aacagtacc gggaaattc atcaaagtg 
      cagttttac cttcagccg cactcttcg gtcccgtct 
      catttattg aaacaagac agactgaaa aaacccgat 
      agattggta gccaatgat tttatccag tgtcgcaaa 
      gttgccgaa catattttg gagaaatta ctgggggat 
      ggaaacgca agttctccc ggaggtgac gccgattcg 
      accactccc agcggcaac gggtacaac gttgatcaa 
      attgaactg acctgcaat gaccaagtc ctggaccca 
      gccatggac ttacgaacc gttaaacat ttcatctgg 
      aaatcttcg gcagacctg acgctgcac tacaggatt 
      gtcaacccc aaataa
    • >TC008107 RB
      atggtaatt tgcgaagct gaggagaaa agacacaga 
      tctggggta aatgccctt cagatagat ccggcatta 
      gaccgtttg tattctgct gggagggac tcaattatt 
      agggtgtat gaacatgag cgctattta cacggaatg 
      gagcaccat actgattgg gttaatgac atagttctg 
      tgttgcgga ggccggcac ttaatctct gcaagttct 
      gacactact gttaaagta tggaacgcc cacaagggc 
      ttctgcatg tcaactctg cgaacacat aaagactat 
      gtgaaagcc ttggcttac gccaaagac agagaacaa 
      gtcgcgtct gcaggtctt gataaatcc atttacttg 
      tgggacatc aacacctta acagcacta acagcgagc 
      aataacaca gtcactact tctagtttg atgggcagc 
      aaagaatca atttatagc ctagcaatg aaccctcca 
      ggcactgtt atagtaagc ggatccacc gagaaagcc 
      ctcaggctt tgggacccg cgaaattgc gtcaagttg 
      tttaagtta aaaggtcac actgataac gttaaggct 
      ttagttgtg tcaaaagat ggcactcat tgcatttct 
      ggcagcagc gatgggtcc atcaaagtg tggagttta 
      gggggccag aggtgtatc caaacaatc agagtgcat 
      agcgactcc gtttgggca cttctggcc acggaaaat 
      ttcactcat gtaatctcc ggtggtagg gacaagaag 
      gtgataatg actgatctg aaaaacccc cagaacagc 
      gttgtggtc tgtgtggag gaggcgcca gtgctcaaa 
      atgtgcttc acggcagac cagcaggct gtctgggtg 
      tcaacttcc aactccagc attcgctgt tggaagttg 
      ccaaccgag aaacacttc aaagaagag gccaaaatg 
      ccgacatcg cccatttcg ctgatacca ggcggtgct 
      gcgatacgt caggccgtc gttctcaac gacaagaga 
      cacattttg accaaagat gccaacgat aatgtcgct 
      ctttacgac gttttgcgt gcgcgaaaa atcgaagac 
      ctaggccaa atcaatttc caagacgaa ataaaaaac 
      cgattcaaa gtagtttac gtcccgaat tggttcaat 
      gttgattta aagactgga atgctgacg attcattta 
      ggacaggac gaagtcgat tgcttctcg gcctgggtt 
      tcggccagg gatgcggga atcagggac tgtccagac 
      cccgaccaa aaagtaaac tacggaaaa ttgctactg 
      caagcactg ttcgagcat tggagaggc gtggagaga 
      gaccccgaa aataaactg tacttctca gtacccaaa 
      catataccc ttgattttg agtgaagtg ggcgggcgc 
      acgctttac cgggtcttg gtgggcgac acagtcgga 
      gacaccgaa aatgcgctc ctgaacgaa acagtgcct 
      aattgggtt atttccatt attgaggag aacagtacc 
      gggaaattc atcaaagtg cagttttac cttcagccg 
      cactcttcg gtcccgtct catttattg aaacaagac 
      agactgaaa aaacccgat agattggta gccaatgat 
      tttatccag tgtcgcaaa gttgccgaa catattttg 
      gagaaatta ctgggggat ggaaacgca agttctccc 
      ggaggtgac gccgattcg accactccc agcggcaac 
      gggtacaac gttgatcaa attgaactg acctgcaat 
      gaccaagtc ctggaccca gccatggac ttacgaacc 
      gttaaacat ttcatctgg aaatcttcg gcagacctg 
      acgctgcac tacaggatt gtcaacccc aaataa
    mRNA sequence
    • >TC008107 RA
      tgtcaaaat aaagtcaga ccgttctaa cctcaagtt 
      tttacaaag cttttcaaa aaagctttt tttacacta 
      atcacgcca atttatagc ccatttctt taaataaaa 
      aatgccaca ttgctaaac ttccaaaaa atggcaaat 
      ctgaagcca ggcgggcaa aatccgcga aaaaaagtc 
      caggtatca atggtaatt tgcgaagct gaggagaaa 
      agacacaga tctggggta aatgccctt cagatagat 
      ccggcatta gaccgtttg tattctgct gggagggac 
      tcaattatt agggtgtat gaacatgag cgctattta 
      cacggaatg gagcaccat actgattgg gttaatgac 
      atagttctg tgttgcgga ggccggcac ttaatctct 
      gcaagttct gacactact gttaaagta tggaacgcc 
      cacaagggc ttctgcatg tcaactctg cgaacacat 
      aaagactat gtgaaagcc ttggcttac gccaaagac 
      agagaacaa gtcgcgtct gcaggtctt gataaatcc 
      atttacttg tgggacatc aacacctta acagcacta 
      acagcgagc aataacaca gtcactact tctagtttg 
      atgggcagc aaagaatca atttatagc ctagcaatg 
      aaccctcca ggcactgtt atagtaagc ggatccacc 
      gagaaagcc ctcaggctt tgggacccg cgaaattgc 
      gtcaagttg tttaagtta aaaggtcac actgataac 
      gttaaggct ttagttgtg tcaaaagat ggcactcat 
      tgcatttct ggcagcagc gatgggtcc atcaaagtg 
      tggagttta gggggccag aggtgtatc caaacaatc 
      agagtgcat agcgactcc gtttgggca cttctggcc 
      acggaaaat ttcactcat gtaatctcc ggtggtagg 
      gacaagaag gtgataatg actgatctg aaaaacccc 
      cagaacagc gttgtggtc tgtgtggag gaggcgcca 
      gtgctcaaa atgtgcttc acggcagac cagcaggct 
      gtctgggtg tcaacttcc aactccagc attcgctgt 
      tggaagttg ccaaccgag aaacacttc aaagaagag 
      gccaaaatg ccgacatcg cccatttcg ctgatacca 
      ggcggtgct gcgatacgt caggccgtc gttctcaac 
      gacaagaga cacattttg accaaagat gccaacgat 
      aatgtcgct ctttacgac gttttgcgt gcgcgaaaa 
      atcgaagac ctaggccaa atcaatttc caagacgaa 
      ataaaaaac cgattcaaa gtagtttac gtcccgaat 
      tggttcaat gttgattta aagactgga atgctgacg 
      attcattta ggacaggac gaagtcgat tgcttctcg 
      gcctgggtt tcggccagg gatgcggga atcagggac 
      tgtccagac cccgaccaa aaagtaaac tacggaaaa 
      ttgctactg caagcactg ttcgagcat tggagaggc 
      gtggagaga gaccccgaa aataaactg tacttctca 
      gtacccaaa catataccc ttgattttg agtgaagtg 
      ggcgggcgc acgctttac cgggtcttg gtgggcgac 
      acagtcgga gacaccgaa aatgcgctc ctgaacgaa 
      acagtgcct aattgggtt atttccatt attgaggag 
      aacagtacc gggaaattc atcaaagtg cagttttac 
      cttcagccg cactcttcg gtcccgtct catttattg 
      aaacaagac agactgaaa aaacccgat agattggta 
      gccaatgat tttatccag tgtcgcaaa gttgccgaa 
      catattttg gagaaatta ctgggggat ggaaacgca 
      agttctccc ggaggtgac gccgattcg accactccc 
      agcggcaac gggtacaac gttgatcaa attgaactg 
      acctgcaat gaccaagtc ctggaccca gccatggac 
      ttacgaacc gttaaacat ttcatctgg aaatcttcg 
      gcagacctg acgctgcac tacaggatt gtcaacccc 
      aaataattc attccttac acttattta tttatccat 
      gattttggt ggacagatt tatactagt ttcttgtaa 
      tcaaacatt tagcagtat atgtttttc gtatttgtt 
      tgcaataaa aatatgtaa cacga
    • >TC008107 RB
      tgtcaaaat aaagtcaga ccgttctaa cctcaagtt 
      tttacaaag cttttcaaa aaagctttt tttacacta 
      atcacgcca atttatagc ccatttctt taaataaaa 
      aatgccaca ttgctaaac ttccaaaaa atggcaaat 
      ctgaagcca ggcgggcaa aatccgcga aaaaaagtc 
      caggttagt tgtttacat tctgtattt acaaaacag 
      ttttttgca acaatcttg gccaggtat caatggtaa 
      tttgcgaag ctgaggaga aaagacaca gatctgggg 
      taaatgccc ttcagatag atccggcat tagaccgtt 
      tgtattctg ctgggaggg actcaatta ttagggtgt 
      atgaacatg agcgctatt tacacggaa tggagcacc 
      atactgatt gggttaatg acatagttc tgtgttgcg 
      gaggccggc acttaatct ctgcaagtt ctgacacta 
      ctgttaaag tatggaacg cccacaagg gcttctgca 
      tgtcaactc tgcgaacac ataaagact atgtgaaag 
      ccttggctt acgccaaag acagagaac aagtcgcgt 
      ctgcaggtc ttgataaat ccatttact tgtgggaca 
      tcaacacct taacagcac taacagcga gcaataaca 
      cagtcacta cttctagtt tgatgggca gcaaagaat 
      caatttata gcctagcaa tgaaccctc caggcactg 
      ttatagtaa gcggatcca ccgagaaag ccctcaggc 
      tttgggacc cgcgaaatt gcgtcaagt tgtttaagt 
      taaaaggtc acactgata acgttaagg ctttagttg 
      tgtcaaaag atggcactc attgcattt ctggcagca 
      gcgatgggt ccatcaaag tgtggagtt tagggggcc 
      agaggtgta tccaaacaa tcagagtgc atagcgact 
      ccgtttggg cacttctgg ccacggaaa atttcactc 
      atgtaatct ccggtggta gggacaaga aggtgataa 
      tgactgatc tgaaaaacc cccagaaca gcgttgtgg 
      tctgtgtgg aggaggcgc cagtgctca aaatgtgct 
      tcacggcag accagcagg ctgtctggg tgtcaactt 
      ccaactcca gcattcgct gttggaagt tgccaaccg 
      agaaacact tcaaagaag aggccaaaa tgccgacat 
      cgcccattt cgctgatac caggcggtg ctgcgatac 
      gtcaggccg tcgttctca acgacaaga gacacattt 
      tgaccaaag atgccaacg ataatgtcg ctctttacg 
      acgttttgc gtgcgcgaa aaatcgaag acctaggcc 
      aaatcaatt tccaagacg aaataaaaa accgattca 
      aagtagttt acgtcccga attggttca atgttgatt 
      taaagactg gaatgctga cgattcatt taggacagg 
      acgaagtcg attgcttct cggcctggg tttcggcca 
      gggatgcgg gaatcaggg actgtccag accccgacc 
      aaaaagtaa actacggaa aattgctac tgcaagcac 
      tgttcgagc attggagag gcgtggaga gagaccccg 
      aaaataaac tgtacttct cagtaccca aacatatac 
      ccttgattt tgagtgaag tgggcgggc gcacgcttt 
      accgggtct tggtgggcg acacagtcg gagacaccg 
      aaaatgcgc tcctgaacg aaacagtgc ctaattggg 
      ttatttcca ttattgagg agaacagta ccgggaaat 
      tcatcaaag tgcagtttt accttcagc cgcactctt 
      cggtcccgt ctcatttat tgaaacaag acagactga 
      aaaaacccg atagattgg tagccaatg attttatcc 
      agtgtcgca aagttgccg aacatattt tggagaaat 
      tactggggg atggaaacg caagttctc ccggaggtg 
      acgccgatt cgaccactc ccagcggca acgggtaca 
      acgttgatc aaattgaac tgacctgca atgaccaag 
      tcctggacc cagccatgg acttacgaa ccgttaaac 
      atttcatct ggaaatctt cggcagacc tgacgctgc 
      actacagga ttgtcaacc ccaaataat tcattcctt 
      acacttatt tatttatcc atgattttg gtggacaga 
      tttatacta gtttcttgt aatcaaaca tttagcagt 
      atatgtttt tcgtatttg tttgcaata aaaatatgt 
      aacacga