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Gene information
General information
TC008107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5040923..5043638
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008107
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008107 RA
      MANLKPGGQ NPRKKVQVS MVICEAEEK RHRSGVNAL 
QIDPALDRL YSAGRDSII RVYEHERYL HGMEHHTDW 
VNDIVLCCG GRHLISASS DTTVKVWNA HKGFCMSTL 
RTHKDYVKA LAYAKDREQ VASAGLDKS IYLWDINTL 
TALTASNNT VTTSSLMGS KESIYSLAM NPPGTVIVS 
GSTEKALRL WDPRNCVKL FKLKGHTDN VKALVVSKD 
GTHCISGSS DGSIKVWSL GGQRCIQTI RVHSDSVWA 
LLATENFTH VISGGRDKK VIMTDLKNP QNSVVVCVE 
EAPVLKMCF TADQQAVWV STSNSSIRC WKLPTEKHF 
KEEAKMPTS PISLIPGGA AIRQAVVLN DKRHILTKD 
ANDNVALYD VLRARKIED LGQINFQDE IKNRFKVVY 
VPNWFNVDL KTGMLTIHL GQDEVDCFS AWVSARDAG 
IRDCPDPDQ KVNYGKLLL QALFEHWRG VERDPENKL 
YFSVPKHIP LILSEVGGR TLYRVLVGD TVGDTENAL 
LNETVPNWV ISIIEENST GKFIKVQFY LQPHSSVPS 
HLLKQDRLK KPDRLVAND FIQCRKVAE HILEKLLGD 
GNASSPGGD ADSTTPSGN GYNVDQIEL TCNDQVLDP 
AMDLRTVKH FIWKSSADL TLHYRIVNP K
    • >TC008107 RB
      MVICEAEEK RHRSGVNAL QIDPALDRL YSAGRDSII 
RVYEHERYL HGMEHHTDW VNDIVLCCG GRHLISASS 
DTTVKVWNA HKGFCMSTL RTHKDYVKA LAYAKDREQ 
VASAGLDKS IYLWDINTL TALTASNNT VTTSSLMGS 
KESIYSLAM NPPGTVIVS GSTEKALRL WDPRNCVKL 
FKLKGHTDN VKALVVSKD GTHCISGSS DGSIKVWSL 
GGQRCIQTI RVHSDSVWA LLATENFTH VISGGRDKK 
VIMTDLKNP QNSVVVCVE EAPVLKMCF TADQQAVWV 
STSNSSIRC WKLPTEKHF KEEAKMPTS PISLIPGGA 
AIRQAVVLN DKRHILTKD ANDNVALYD VLRARKIED 
LGQINFQDE IKNRFKVVY VPNWFNVDL KTGMLTIHL 
GQDEVDCFS AWVSARDAG IRDCPDPDQ KVNYGKLLL 
QALFEHWRG VERDPENKL YFSVPKHIP LILSEVGGR 
TLYRVLVGD TVGDTENAL LNETVPNWV ISIIEENST 
GKFIKVQFY LQPHSSVPS HLLKQDRLK KPDRLVAND 
FIQCRKVAE HILEKLLGD GNASSPGGD ADSTTPSGN 
GYNVDQIEL TCNDQVLDP AMDLRTVKH FIWKSSADL 
TLHYRIVNP K
    CDS sequence
    • >TC008107 RA
      atggcaaat ctgaagcca ggcgggcaa aatccgcga 
aaaaaagtc caggtatca atggtaatt tgcgaagct 
gaggagaaa agacacaga tctggggta aatgccctt 
cagatagat ccggcatta gaccgtttg tattctgct 
gggagggac tcaattatt agggtgtat gaacatgag 
cgctattta cacggaatg gagcaccat actgattgg 
gttaatgac atagttctg tgttgcgga ggccggcac 
ttaatctct gcaagttct gacactact gttaaagta 
tggaacgcc cacaagggc ttctgcatg tcaactctg 
cgaacacat aaagactat gtgaaagcc ttggcttac 
gccaaagac agagaacaa gtcgcgtct gcaggtctt 
gataaatcc atttacttg tgggacatc aacacctta 
acagcacta acagcgagc aataacaca gtcactact 
tctagtttg atgggcagc aaagaatca atttatagc 
ctagcaatg aaccctcca ggcactgtt atagtaagc 
ggatccacc gagaaagcc ctcaggctt tgggacccg 
cgaaattgc gtcaagttg tttaagtta aaaggtcac 
actgataac gttaaggct ttagttgtg tcaaaagat 
ggcactcat tgcatttct ggcagcagc gatgggtcc 
atcaaagtg tggagttta gggggccag aggtgtatc 
caaacaatc agagtgcat agcgactcc gtttgggca 
cttctggcc acggaaaat ttcactcat gtaatctcc 
ggtggtagg gacaagaag gtgataatg actgatctg 
aaaaacccc cagaacagc gttgtggtc tgtgtggag 
gaggcgcca gtgctcaaa atgtgcttc acggcagac 
cagcaggct gtctgggtg tcaacttcc aactccagc 
attcgctgt tggaagttg ccaaccgag aaacacttc 
aaagaagag gccaaaatg ccgacatcg cccatttcg 
ctgatacca ggcggtgct gcgatacgt caggccgtc 
gttctcaac gacaagaga cacattttg accaaagat 
gccaacgat aatgtcgct ctttacgac gttttgcgt 
gcgcgaaaa atcgaagac ctaggccaa atcaatttc 
caagacgaa ataaaaaac cgattcaaa gtagtttac 
gtcccgaat tggttcaat gttgattta aagactgga 
atgctgacg attcattta ggacaggac gaagtcgat 
tgcttctcg gcctgggtt tcggccagg gatgcggga 
atcagggac tgtccagac cccgaccaa aaagtaaac 
tacggaaaa ttgctactg caagcactg ttcgagcat 
tggagaggc gtggagaga gaccccgaa aataaactg 
tacttctca gtacccaaa catataccc ttgattttg 
agtgaagtg ggcgggcgc acgctttac cgggtcttg 
gtgggcgac acagtcgga gacaccgaa aatgcgctc 
ctgaacgaa acagtgcct aattgggtt atttccatt 
attgaggag aacagtacc gggaaattc atcaaagtg 
cagttttac cttcagccg cactcttcg gtcccgtct 
catttattg aaacaagac agactgaaa aaacccgat 
agattggta gccaatgat tttatccag tgtcgcaaa 
gttgccgaa catattttg gagaaatta ctgggggat 
ggaaacgca agttctccc ggaggtgac gccgattcg 
accactccc agcggcaac gggtacaac gttgatcaa 
attgaactg acctgcaat gaccaagtc ctggaccca 
gccatggac ttacgaacc gttaaacat ttcatctgg 
aaatcttcg gcagacctg acgctgcac tacaggatt 
gtcaacccc aaataa
    • >TC008107 RB
      atggtaatt tgcgaagct gaggagaaa agacacaga 
tctggggta aatgccctt cagatagat ccggcatta 
gaccgtttg tattctgct gggagggac tcaattatt 
agggtgtat gaacatgag cgctattta cacggaatg 
gagcaccat actgattgg gttaatgac atagttctg 
tgttgcgga ggccggcac ttaatctct gcaagttct 
gacactact gttaaagta tggaacgcc cacaagggc 
ttctgcatg tcaactctg cgaacacat aaagactat 
gtgaaagcc ttggcttac gccaaagac agagaacaa 
gtcgcgtct gcaggtctt gataaatcc atttacttg 
tgggacatc aacacctta acagcacta acagcgagc 
aataacaca gtcactact tctagtttg atgggcagc 
aaagaatca atttatagc ctagcaatg aaccctcca 
ggcactgtt atagtaagc ggatccacc gagaaagcc 
ctcaggctt tgggacccg cgaaattgc gtcaagttg 
tttaagtta aaaggtcac actgataac gttaaggct 
ttagttgtg tcaaaagat ggcactcat tgcatttct 
ggcagcagc gatgggtcc atcaaagtg tggagttta 
gggggccag aggtgtatc caaacaatc agagtgcat 
agcgactcc gtttgggca cttctggcc acggaaaat 
ttcactcat gtaatctcc ggtggtagg gacaagaag 
gtgataatg actgatctg aaaaacccc cagaacagc 
gttgtggtc tgtgtggag gaggcgcca gtgctcaaa 
atgtgcttc acggcagac cagcaggct gtctgggtg 
tcaacttcc aactccagc attcgctgt tggaagttg 
ccaaccgag aaacacttc aaagaagag gccaaaatg 
ccgacatcg cccatttcg ctgatacca ggcggtgct 
gcgatacgt caggccgtc gttctcaac gacaagaga 
cacattttg accaaagat gccaacgat aatgtcgct 
ctttacgac gttttgcgt gcgcgaaaa atcgaagac 
ctaggccaa atcaatttc caagacgaa ataaaaaac 
cgattcaaa gtagtttac gtcccgaat tggttcaat 
gttgattta aagactgga atgctgacg attcattta 
ggacaggac gaagtcgat tgcttctcg gcctgggtt 
tcggccagg gatgcggga atcagggac tgtccagac 
cccgaccaa aaagtaaac tacggaaaa ttgctactg 
caagcactg ttcgagcat tggagaggc gtggagaga 
gaccccgaa aataaactg tacttctca gtacccaaa 
catataccc ttgattttg agtgaagtg ggcgggcgc 
acgctttac cgggtcttg gtgggcgac acagtcgga 
gacaccgaa aatgcgctc ctgaacgaa acagtgcct 
aattgggtt atttccatt attgaggag aacagtacc 
gggaaattc atcaaagtg cagttttac cttcagccg 
cactcttcg gtcccgtct catttattg aaacaagac 
agactgaaa aaacccgat agattggta gccaatgat 
tttatccag tgtcgcaaa gttgccgaa catattttg 
gagaaatta ctgggggat ggaaacgca agttctccc 
ggaggtgac gccgattcg accactccc agcggcaac 
gggtacaac gttgatcaa attgaactg acctgcaat 
gaccaagtc ctggaccca gccatggac ttacgaacc 
gttaaacat ttcatctgg aaatcttcg gcagacctg 
acgctgcac tacaggatt gtcaacccc aaataa
    mRNA sequence
    • >TC008107 RA
      tgtcaaaat aaagtcaga ccgttctaa cctcaagtt 
tttacaaag cttttcaaa aaagctttt tttacacta 
atcacgcca atttatagc ccatttctt taaataaaa 
aatgccaca ttgctaaac ttccaaaaa atggcaaat 
ctgaagcca ggcgggcaa aatccgcga aaaaaagtc 
caggtatca atggtaatt tgcgaagct gaggagaaa 
agacacaga tctggggta aatgccctt cagatagat 
ccggcatta gaccgtttg tattctgct gggagggac 
tcaattatt agggtgtat gaacatgag cgctattta 
cacggaatg gagcaccat actgattgg gttaatgac 
atagttctg tgttgcgga ggccggcac ttaatctct 
gcaagttct gacactact gttaaagta tggaacgcc 
cacaagggc ttctgcatg tcaactctg cgaacacat 
aaagactat gtgaaagcc ttggcttac gccaaagac 
agagaacaa gtcgcgtct gcaggtctt gataaatcc 
atttacttg tgggacatc aacacctta acagcacta 
acagcgagc aataacaca gtcactact tctagtttg 
atgggcagc aaagaatca atttatagc ctagcaatg 
aaccctcca ggcactgtt atagtaagc ggatccacc 
gagaaagcc ctcaggctt tgggacccg cgaaattgc 
gtcaagttg tttaagtta aaaggtcac actgataac 
gttaaggct ttagttgtg tcaaaagat ggcactcat 
tgcatttct ggcagcagc gatgggtcc atcaaagtg 
tggagttta gggggccag aggtgtatc caaacaatc 
agagtgcat agcgactcc gtttgggca cttctggcc 
acggaaaat ttcactcat gtaatctcc ggtggtagg 
gacaagaag gtgataatg actgatctg aaaaacccc 
cagaacagc gttgtggtc tgtgtggag gaggcgcca 
gtgctcaaa atgtgcttc acggcagac cagcaggct 
gtctgggtg tcaacttcc aactccagc attcgctgt 
tggaagttg ccaaccgag aaacacttc aaagaagag 
gccaaaatg ccgacatcg cccatttcg ctgatacca 
ggcggtgct gcgatacgt caggccgtc gttctcaac 
gacaagaga cacattttg accaaagat gccaacgat 
aatgtcgct ctttacgac gttttgcgt gcgcgaaaa 
atcgaagac ctaggccaa atcaatttc caagacgaa 
ataaaaaac cgattcaaa gtagtttac gtcccgaat 
tggttcaat gttgattta aagactgga atgctgacg 
attcattta ggacaggac gaagtcgat tgcttctcg 
gcctgggtt tcggccagg gatgcggga atcagggac 
tgtccagac cccgaccaa aaagtaaac tacggaaaa 
ttgctactg caagcactg ttcgagcat tggagaggc 
gtggagaga gaccccgaa aataaactg tacttctca 
gtacccaaa catataccc ttgattttg agtgaagtg 
ggcgggcgc acgctttac cgggtcttg gtgggcgac 
acagtcgga gacaccgaa aatgcgctc ctgaacgaa 
acagtgcct aattgggtt atttccatt attgaggag 
aacagtacc gggaaattc atcaaagtg cagttttac 
cttcagccg cactcttcg gtcccgtct catttattg 
aaacaagac agactgaaa aaacccgat agattggta 
gccaatgat tttatccag tgtcgcaaa gttgccgaa 
catattttg gagaaatta ctgggggat ggaaacgca 
agttctccc ggaggtgac gccgattcg accactccc 
agcggcaac gggtacaac gttgatcaa attgaactg 
acctgcaat gaccaagtc ctggaccca gccatggac 
ttacgaacc gttaaacat ttcatctgg aaatcttcg 
gcagacctg acgctgcac tacaggatt gtcaacccc 
aaataattc attccttac acttattta tttatccat 
gattttggt ggacagatt tatactagt ttcttgtaa 
tcaaacatt tagcagtat atgtttttc gtatttgtt 
tgcaataaa aatatgtaa cacga
    • >TC008107 RB
      tgtcaaaat aaagtcaga ccgttctaa cctcaagtt 
tttacaaag cttttcaaa aaagctttt tttacacta 
atcacgcca atttatagc ccatttctt taaataaaa 
aatgccaca ttgctaaac ttccaaaaa atggcaaat 
ctgaagcca ggcgggcaa aatccgcga aaaaaagtc 
caggttagt tgtttacat tctgtattt acaaaacag 
ttttttgca acaatcttg gccaggtat caatggtaa 
tttgcgaag ctgaggaga aaagacaca gatctgggg 
taaatgccc ttcagatag atccggcat tagaccgtt 
tgtattctg ctgggaggg actcaatta ttagggtgt 
atgaacatg agcgctatt tacacggaa tggagcacc 
atactgatt gggttaatg acatagttc tgtgttgcg 
gaggccggc acttaatct ctgcaagtt ctgacacta 
ctgttaaag tatggaacg cccacaagg gcttctgca 
tgtcaactc tgcgaacac ataaagact atgtgaaag 
ccttggctt acgccaaag acagagaac aagtcgcgt 
ctgcaggtc ttgataaat ccatttact tgtgggaca 
tcaacacct taacagcac taacagcga gcaataaca 
cagtcacta cttctagtt tgatgggca gcaaagaat 
caatttata gcctagcaa tgaaccctc caggcactg 
ttatagtaa gcggatcca ccgagaaag ccctcaggc 
tttgggacc cgcgaaatt gcgtcaagt tgtttaagt 
taaaaggtc acactgata acgttaagg ctttagttg 
tgtcaaaag atggcactc attgcattt ctggcagca 
gcgatgggt ccatcaaag tgtggagtt tagggggcc 
agaggtgta tccaaacaa tcagagtgc atagcgact 
ccgtttggg cacttctgg ccacggaaa atttcactc 
atgtaatct ccggtggta gggacaaga aggtgataa 
tgactgatc tgaaaaacc cccagaaca gcgttgtgg 
tctgtgtgg aggaggcgc cagtgctca aaatgtgct 
tcacggcag accagcagg ctgtctggg tgtcaactt 
ccaactcca gcattcgct gttggaagt tgccaaccg 
agaaacact tcaaagaag aggccaaaa tgccgacat 
cgcccattt cgctgatac caggcggtg ctgcgatac 
gtcaggccg tcgttctca acgacaaga gacacattt 
tgaccaaag atgccaacg ataatgtcg ctctttacg 
acgttttgc gtgcgcgaa aaatcgaag acctaggcc 
aaatcaatt tccaagacg aaataaaaa accgattca 
aagtagttt acgtcccga attggttca atgttgatt 
taaagactg gaatgctga cgattcatt taggacagg 
acgaagtcg attgcttct cggcctggg tttcggcca 
gggatgcgg gaatcaggg actgtccag accccgacc 
aaaaagtaa actacggaa aattgctac tgcaagcac 
tgttcgagc attggagag gcgtggaga gagaccccg 
aaaataaac tgtacttct cagtaccca aacatatac 
ccttgattt tgagtgaag tgggcgggc gcacgcttt 
accgggtct tggtgggcg acacagtcg gagacaccg 
aaaatgcgc tcctgaacg aaacagtgc ctaattggg 
ttatttcca ttattgagg agaacagta ccgggaaat 
tcatcaaag tgcagtttt accttcagc cgcactctt 
cggtcccgt ctcatttat tgaaacaag acagactga 
aaaaacccg atagattgg tagccaatg attttatcc 
agtgtcgca aagttgccg aacatattt tggagaaat 
tactggggg atggaaacg caagttctc ccggaggtg 
acgccgatt cgaccactc ccagcggca acgggtaca 
acgttgatc aaattgaac tgacctgca atgaccaag 
tcctggacc cagccatgg acttacgaa ccgttaaac 
atttcatct ggaaatctt cggcagacc tgacgctgc 
actacagga ttgtcaacc ccaaataat tcattcctt 
acacttatt tatttatcc atgattttg gtggacaga 
tttatacta gtttcttgt aatcaaaca tttagcagt 
atatgtttt tcgtatttg tttgcaata aaaatatgt 
aacacga
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