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Gene information
General information
TC008058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4413392..4415336
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008058
    iBeetle screen iB_01307
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: increased amount of presumably dead larvae shortly before/after hatching, without muscle pattern but diffuse fluorescence
    larval musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: increased amount of presumably dead larvae shortly before/after hatching, without muscle pattern but diffuse fluorescence
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008058 RA
      MSTTVPTPD ISTPSGILS ASNVFYFLL IPALLLWYA 
      YWRISKRHM LELAAKIPG PPGLPILGN ALDLVGKPH 
      QVFSHVYQK SFEYKKVVK MWAGPKLLV FLTDPSDIE 
      LILSSYVHI DKSSEYRFF KPWLGDGLL ISTGQKWKA 
      HRKLIAPTF HLNVLKSFI DLFNANSRD VIRKLQKEI 
      GKEFDCHDY MSEATVEML LETAMGVSK KTQDQSGYD 
      YAMAVMKMC DILHLRHTK FWLRPDIIF NQTKYAEYQ 
      KSLINTIHS LTRKVIKRK RADFDKGIR GSTAEVPPE 
      LQTKNYDKT ESKTVVEGL SYGQSAGLK DDLDVDDND 
      IGEKKRMAF LDLMIEASQ NGVVINDEE IKEQVDTIM 
      FEGHDTTAA GSSFFLSMM GVHQDIQDK VVQELYDIF 
      GDSDRPATF ADTLEMKYL ERCLMETLR MFPPVPIIA 
      RQLNQDLKL ASGDYTVPA GCTVVIGTF KVHRLEEYY 
      PNPDKFDPD NFLPERTAN RHYYSFIPF SAGPRSCVG 
      RKYAMLKLK ILLSTILRN YRIYSDLKE KDFQLQGDI 
      ILKRAEGFK VRLEPRKMA KA
    CDS sequence
    • >TC008058 RA
      atgtctacg accgtacca acaccagac ataagtacc 
      ccttcgggg atactttcc gcctccaat gtcttctac 
      tttttgcta attccggcc ctgctgtta tggtacgct 
      tattggaga atatcaaag agacacatg ctagagctc 
      gccgccaaa atccccgga cccccggga ttaccaatc 
      ctcggaaac gccctagac ctggtcggg aaaccacac 
      caggtcttc agccatgtg taccaaaag tccttcgag 
      tacaaaaag gtggtgaaa atgtgggcg ggccccaaa 
      cttctggtc tttctgacc gacccttcg gacatcgag 
      ctgatcttg agcagttac gtccacatt gacaagtcg 
      tccgagtac aggtttttc aaaccgtgg ctcggcgat 
      ggtcttttg atttccacc ggacagaag tggaaagcc 
      catcgcaaa ctcatcgcc cccacgttc catctcaac 
      gtgctcaag tccttcatc gacttgttc aacgccaac 
      agtcgcgac gttattcga aaattgcaa aaggaaatc 
      ggcaaagaa ttcgactgt cacgactac atgtctgag 
      gccaccgtt gagatgctc ctcgaaacg gcgatgggt 
      gtgagcaag aagacccaa gaccaaagc ggctacgac 
      tacgccatg gctgttatg aaaatgtgc gatattttg 
      cacttgaga cacactaaa ttctggctg aggcctgat 
      atcatcttc aaccaaacg aaatacgcc gaatatcag 
      aaatccctg attaacacc atccacagt ttgacccgc 
      aaggtcatt aaacgtaaa agggccgac tttgacaaa 
      ggcatcaga ggctcaact gccgaggtc ccgcctgag 
      ctccaaacg aaaaattac gacaaaact gaatcgaaa 
      accgtggtt gaaggactc tcgtacgga caaagtgcc 
      ggccttaag gacgatctg gacgtggac gataacgac 
      atcggcgaa aagaaacgc atggccttt ttggatctg 
      atgattgaa gcttcgcaa aacggcgtt gttatcaac 
      gatgaggaa attaaggaa caagtcgac acgatcatg 
      tttgaaggc cacgacacc acggccgct ggcagcagc 
      ttcttcctc tcgatgatg ggcgtccac caggacatc 
      caggacaaa gtggtacaa gaactgtac gatatcttt 
      ggcgactct gaccggcct gcaactttc gccgacact 
      ctcgaaatg aagtacttg gaacggtgt cttatggaa 
      actttgagg atgttccct ccggtaccc attatcgcg 
      cggcaatta aaccaagac ttgaaatta gcatctggt 
      gattacact gttcctgcc ggttgtact gtcgttatt 
      ggtactttc aaagttcat cgccttgag gagtattac 
      cctaacccg gataaattc gatcctgat aacttcctt 
      ccggagagg actgccaac aggcactac tactccttt 
      attccgttt agtgccgga cctcgcagc tgtgtcggg 
      cggaaatat gccatgttg aaactgaaa atactgctc 
      tctacgatt ttgagaaac taccgaatt tattcggat 
      ttgaaagaa aaagatttc caactccaa ggtgatatt 
      attcttaag agggccgaa ggatttaaa gtcagattg 
      gaacccagg aagatggcc aaagcctaa
    mRNA sequence
    • >TC008058 RA
      cacattttg gtggcagtc gtctgttta gtgatgtct 
      acgaccgta ccaacacca gacataagt accccttcg 
      gggatactt tccgcctcc aatgtcttc tactttttg 
      ctaattccg gccctgctg ttatggtac gcttattgg 
      agaatatca aagagacac atgctagag ctcgccgcc 
      aaaatcccc ggacccccg ggattacca atcctcgga 
      aacgcccta gacctggtc gggaaacca caccaggtc 
      ttcagccat gtgtaccaa aagtccttc gagtacaaa 
      aaggtggtg aaaatgtgg gcgggcccc aaacttctg 
      gtctttctg accgaccct tcggacatc gagctgatc 
      ttgagcagt tacgtccac attgacaag tcgtccgag 
      tacaggttt ttcaaaccg tggctcggc gatggtctt 
      ttgatttcc accggacag aagtggaaa gcccatcgc 
      aaactcatc gcccccacg ttccatctc aacgtgctc 
      aagtccttc atcgacttg ttcaacgcc aacagtcgc 
      gacgttatt cgaaaattg caaaaggaa atcggcaaa 
      gaattcgac tgtcacgac tacatgtct gaggccacc 
      gttgagatg ctcctcgaa acggcgatg ggtgtgagc 
      aagaagacc caagaccaa agcggctac gactacgcc 
      atggctgtt atgaaaatg tgcgatatt ttgcacttg 
      agacacact aaattctgg ctgaggcct gatatcatc 
      ttcaaccaa acgaaatac gccgaatat cagaaatcc 
      ctgattaac accatccac agtttgacc cgcaaggtc 
      attaaacgt aaaagggcc gactttgac aaaggcatc 
      agaggctca actgccgag gtcccgcct gagctccaa 
      acgaaaaat tacgacaaa actgaatcg aaaaccgtg 
      gttgaagga ctctcgtac ggacaaagt gccggcctt 
      aaggacgat ctggacgtg gacgataac gacatcggc 
      gaaaagaaa cgcatggcc tttttggat ctgatgatt 
      gaagcttcg caaaacggc gttgttatc aacgatgag 
      gaaattaag gaacaagtc gacacgatc atgtttgaa 
      ggccacgac accacggcc gctggcagc agcttcttc 
      ctctcgatg atgggcgtc caccaggac atccaggac 
      aaagtggta caagaactg tacgatatc tttggcgac 
      tctgaccgg cctgcaact ttcgccgac actctcgaa 
      atgaagtac ttggaacgg tgtcttatg gaaactttg 
      aggatgttc cctccggta cccattatc gcgcggcaa 
      ttaaaccaa gacttgaaa ttagcatct ggtgattac 
      actgttcct gccggttgt actgtcgtt attggtact 
      ttcaaagtt catcgcctt gaggagtat taccctaac 
      ccggataaa ttcgatcct gataacttc cttccggag 
      aggactgcc aacaggcac tactactcc tttattccg 
      tttagtgcc ggacctcgc agctgtgtc gggcggaaa 
      tatgccatg ttgaaactg aaaatactg ctctctacg 
      attttgaga aactaccga atttattcg gatttgaaa 
      gaaaaagat ttccaactc caaggtgat attattctt 
      aagagggcc gaaggattt aaagtcaga ttggaaccc 
      aggaagatg gccaaagcc taaatcgaa attatttat 
      gcgtagttg tagatttgt atatttgta gaaaattgg 
      gcttaaatt atatttatg acaaataga gctagttga 
      cgaacat