Status
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Gene information
General information
TC008043 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4253547..4258119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008043
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01306

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature spotted - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: dorsal cuticle seems to be slightly invaginated, causing loss of some bristles.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: dorsal cuticle seems to be slightly invaginated, causing loss of some bristles.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008043 RA
      MKLLRLFSC CLFFFTGAT ALPVGLLYS HNVQEARKL 
PQENDVSSP EIKLKVPVV FYGTKFNSI FVNSNGFVS 
FQTEIPNFF NIEFPLDYP LIAPFYTNV DTRSAGTIS 
YYETSDSAL IHRATENVR EYFSDQENF QAKSLFIVT 
WFRVGYFNR GSDKTNTYQ LVIITDGAS SFVELLYPK 
EGIQWIQGT GDESGLPDA RAQAGFISA EGKMYTLPG 
SGTEQVRNL EVWSNIDLP GQFMYRVDG NDVIGPDLI 
TDDLKSDEN PQTCAEATT YCHVQANCV DYEEGFCCQ 
CKKQYYGNG RFCVKKDVP LRVNGKING KINGERLEN 
LDLQSYIVM VDGRAYTAI SKIPESIGF DIQSLQILG 
GVIGYIFAK PIRNAQNGY QFTGGVFNH TAILKFLNT 
SQTVTIKQK YLGLDVFDQ LRLETNIQG EIPTLPFDS 
KIEIDEYQE QYTLTSPGV VQMSSTRFF KYLDENKEE 
TTVSYDIEQ VYTFDYCKH ENTSLGESW KLKVGKNFI 
SYESKEQII RFGLTNKIM PLGEIDPCQ EGRSQCAPF 
SACVVDGDS FRCVCNPGF QQMFAENET VCADIDECQ 
TGLHSCDQN AACVNQVGS YSCSCNPGF TGNGQVCEN 
EFSCHNVIC PPNAECVEG DNVAMCRCM PGFTGDGQV 
CSPLVDRSC HTANNCDPF AVCTIDPKT NSYYCVCLP 
GYEGDGYSC SKVEVQNVT EAAVEKEIQ RCVLGVCWC 
PEGYTFEKG TTYCIPKEE TTVLPPTTP LADNVTCDI 
LNNCNANAQ CIYNSENET FSCICNEGY DGDGYTCDA 
VTVSCAQEY NCGPNANCY YDETVGKSK CVCNPGYFG 
DGFNCTITA ICTSNADCT QTEECLLSS SQRYECVCK 
EGYVRDSQN QCIKPSTCG GGLCVENAE CLYDDTYQL 
HYCSCKSGY MGDGITECK PRPIGCNIE NKCGLHATC 
EYDPSTSLY ECQCERGYY GDGYVCYTE INCHIEPTL 
CDPQATCVT DSNRRYVCQ CNTGYVGNG TICRKNPTH 
EGNFLLLNQ GMSTLKIPF EPTKQNSGK PIQVKYFQT 
AVGLDIDCY EGRVYWGDI SGRAIRSSS YNGSNKSDF 
IVEGIGSPE GLAVDWVSR NIYWTDSTK DTVEVASIE 
TRRRRVLFD FNLVNPRGI AVHPQRGKI FWTDWDRKN 
PKIEWANGD GSGRGIFLQ GEAVSLPNS LTIDYDTEQ 
LCYADAGTK KIECVDIDS KIRRTIATN CTYPFGITV 
TDKHIYWSD WITKKIERA EKNTLTRLP PLQVPLGGS 
GNKLFGLVA VPQSCPQLT NVCQYYKDQ CPVDHICLP 
NGAGSRTCV CTFKADSSE TPSCKL
    CDS sequence
    • >TC008043 RA
      atgaagttg cttcgactt ttctcttgt tgcctattt 
tttttcacg ggcgcgacc gccttacca gtcggactc 
ttatacagt cataatgtg caagaagcc cgcaaattg 
ccacaggaa aatgacgtc tccagcccg gaaatcaag 
ctcaaagtg cccgtggtt ttctacgga acgaaattc 
aactcgatt tttgtgaac tcaaacggc ttcgtctcc 
ttccaaacc gaaatcccc aacttcttc aacatcgag 
ttccccctc gactacccc ctcatcgcc cccttttac 
acgaacgtg gacaccagg agcgctggg acgatttcc 
tactacgaa acctccgac tcggccctc attcaccgc 
gccacagaa aacgtccgt gaatacttc tccgaccag 
gaaaatttc caggccaaa agtctattt atcgtgact 
tggttccgt gtcggttat ttcaacaga ggcagcgac 
aaaaccaac acttatcaa ctggtgatc atcaccgac 
ggcgccagc tcattcgtt gaactcctc taccccaaa 
gagggcatc cagtggatc caaggcacc ggggacgag 
tccgggctc ccggacgcc cgcgcccaa gccggattc 
atctccgcc gagggcaaa atgtacacg cttccgggc 
tccggcacg gagcaagtc cggaatttg gaagtgtgg 
tccaatatt gacctcccg gggcagttc atgtacagg 
gttgatggg aatgacgtg atcgggccc gatttgatc 
accgatgat ttgaaaagc gacgagaat ccgcaaacg 
tgtgccgaa gctacgact tattgtcac gtccaagcc 
aactgtgtg gactacgag gaggggttc tgctgccag 
tgcaagaaa cagtactac gggaatggg cggttttgt 
gtaaagaaa gacgttccg ctgagagtt aatgggaaa 
atcaacggg aaaattaac ggcgaacga ctggaaaat 
ttggacttg cagtcgtac attgtcatg gtcgatggc 
cgggcctac acggctatt agcaaaatc cccgaatct 
ataggtttt gacatccaa tccttgcaa attctgggt 
ggggtaatt ggttatatt ttcgccaaa cccatcaga 
aacgcccaa aatgggtac caattcacg ggaggtgtt 
ttcaaccac actgccatt ctcaaattc ttaaacacc 
agtcaaacc gttacgata aaacaaaaa tatttaggt 
ttggacgtt ttcgatcaa ttgcgactc gaaaccaac 
attcagggg gaaatcccg actttaccg ttcgactca 
aaaatcgaa atcgatgag tatcaggaa cagtacact 
ttgacgagt cccggagtg gttcaaatg tcatcgact 
cgctttttc aaatacttg gacgaaaac aaagaagag 
accaccgtt tcgtacgac attgaacaa gtctacact 
tttgattac tgcaagcac gagaatacc tcactcggc 
gagagctgg aagctcaaa gtcggcaaa aacttcatc 
agttacgag agcaaagaa cagatcatc cgtttcggc 
ttaactaac aagatcatg cctttgggg gagatcgac 
ccttgccag gagggccgc tcccagtgc gcccctttc 
agtgcctgc gtcgtggac ggcgacagc ttccggtgc 
gtttgcaac ccaggtttc cagcaaatg tttgccgaa 
aacgagaca gtctgcgcc gacattgac gaatgccag 
accgggctc cacagttgc gaccagaac gcagcctgt 
gtgaaccag gtcgggtcg tactcctgc tcttgcaat 
ccgggcttc accggaaac gggcaagtg tgcgagaac 
gaattctcg tgccataac gtcatttgc cccccgaat 
gccgagtgc gtggagggc gacaacgtg gcaatgtgc 
cgctgcatg cccggattc accggagac gggcaagtt 
tgtagcccg ctggtcgac cggagttgc cacacggcc 
aacaactgc gaccccttc gccgtctgc accatcgac 
cccaagacg aacagctac tactgtgtg tgtttgccg 
gggtacgaa ggggacggc tacagttgt agcaaggtg 
gaagtgcaa aacgtgacg gaagcggcg gtcgagaag 
gagatccag aggtgcgtc ctgggagtg tgttggtgc 
cccgaaggg tacactttt gagaaaggg accacgtat 
tgcatcccc aaagaggag acgactgtt ctaccgccg 
actacgcca cttgcggat aatgtcacg tgtgatatt 
ttgaataat tgtaacgcc aacgctcag tgtatttac 
aattcggaa aatgaaact ttctcgtgt atctgtaac 
gaaggttac gacggggat ggttacacc tgcgatgct 
gtaaccgtg tcgtgtgcg caggaatat aactgtggg 
cccaatgca aattgttac tacgacgaa actgtcggg 
aagtcgaaa tgtgtgtgc aacccgggg tatttcggg 
gacggtttc aactgcaca atcacagcc atttgcacc 
tccaatgcc gactgcacc caaaccgag gagtgcctt 
ctgtccagc tcacagcgc tacgaatgc gtctgtaaa 
gagggctac gtccgggat tcccaaaac cagtgcatt 
aaacccagc acttgtggc ggcggactt tgcgtcgaa 
aacgccgag tgtctctac gacgacacc taccagctc 
cattattgc tcctgcaag agtggttac atgggggac 
ggcataacc gaatgtaaa ccgcgcccc atcggttgt 
aacattgaa aataaatgc ggcctccac gccacctgc 
gagtacgac ccctccacc tccttgtac gagtgtcag 
tgtgaacgt ggttactac ggcgatggt tacgtgtgt 
tacaccgaa atcaactgt cacatcgag ccaactttg 
tgcgaccca caagccacg tgtgttaca gactcgaac 
cggaggtac gtctgtcag tgtaacact ggctacgtg 
gggaacgga accatctgt cggaagaat cccacccac 
gaaggcaac ttcctgtta ctcaaccag ggaatgtca 
acactaaaa atcccattc gaacccacg aagcaaaat 
tcaggaaaa cccatccag gtcaagtac ttccaaact 
gctgtgggc ctggacatt gactgttac gaaggacgg 
gtctattgg ggtgacatc agcgggagg gcgatcagg 
agctcctcc tacaacggg agtaacaag agcgacttt 
atcgtggaa gggattggg tcaccggaa ggactagca 
gttgattgg gtttcaaga aacatttat tggaccgat 
tcgaccaaa gatacagtc gaagtcgcg agtattgag 
acgaggcgg aggcgagtc cttttcgat ttcaattta 
gtcaacccg agaggaatc gccgtgcat ccgcaacga 
ggcaaaatc ttttggacc gattgggac cgcaagaac 
cccaagatc gagtgggcg aacggcgac ggctccggc 
cgcggcatt ttcctgcaa ggggaagcc gtttccctt 
cccaactct ctcaccatt gactacgac accgaacaa 
ttgtgttac gcggacgcc ggaacgaaa aaaatcgaa 
tgtgtcgat atcgacagc aaaatcagg cgcacaatt 
gccaccaac tgcacatat ccattcggg ataacagtc 
accgataaa cacatctat tggtcggac tggatcacg 
aagaaaatt gaacgggcg gagaaaaac acgctgaca 
cgtttgccg cccctgcag gttcctctc ggcggcagt 
gggaacaag ttgtttggg ttggtggca gtgcctcag 
agttgtccc cagttgaca aacgtgtgc caatattac 
aaggaccaa tgccctgtc gatcacatt tgtctcccg 
aacggcgca ggaagtcgg acttgtgtc tgcactttc 
aaagccgac agttctgaa acaccttct tgcaaatta 
taa
    mRNA sequence
    • >TC008043 RA
      atacgagcg attatttta agtcagtta tagtcagtg 
gctttcgaa aatgaagtt gcttcgact tttctcttg 
ttgcctatt ttttttcac gggcgcgac cgccttacc 
agtcggact cttatacag tcataatgt gcaagaagc 
ccgcaaatt gccacagga aaatgacgt ctccagccc 
ggaaatcaa gctcaaagt gcccgtggt tttctacgg 
aacgaaatt caactcgat ttttgtgaa ctcaaacgg 
cttcgtctc cttccaaac cgaaatccc caacttctt 
caacatcga gttccccct cgactaccc cctcatcgc 
cccctttta cacgaacgt ggacaccag gagcgctgg 
gacgatttc ctactacga aacctccga ctcggccct 
cattcaccg cgccacaga aaacgtccg tgaatactt 
ctccgacca ggaaaattt ccaggccaa aagtctatt 
tatcgtgac ttggttccg tgtcggtta tttcaacag 
aggcagcga caaaaccaa cacttatca actggtgat 
catcaccga cggcgccag ctcattcgt tgaactcct 
ctaccccaa agagggcat ccagtggat ccaaggcac 
cggggacga gtccgggct cccggacgc ccgcgccca 
agccggatt catctccgc cgagggcaa aatgtacac 
gcttccggg ctccggcac ggagcaagt ccggaattt 
ggaagtgtg gtccaatat tgacctccc ggggcagtt 
catgtacag ggttgatgg gaatgacgt gatcgggcc 
cgatttgat caccgatga tttgaaaag cgacgagaa 
tccgcaaac gtgtgccga agctacgac ttattgtca 
cgtccaagc caactgtgt ggactacga ggaggggtt 
ctgctgcca gtgcaagaa acagtacta cgggaatgg 
gcggttttg tgtaaagaa agacgttcc gctgagagt 
taatgggaa aatcaacgg gaaaattaa cggcgaacg 
actggaaaa tttggactt gcagtcgta cattgtcat 
ggtcgatgg ccgggccta cacggctat tagcaaaat 
ccccgaatc tataggttt tgacatcca atccttgca 
aattctggg tggggtaat tggttatat tttcgccaa 
acccatcag aaacgccca aaatgggta ccaattcac 
gggaggtgt tttcaacca cactgccat tctcaaatt 
cttaaacac cagtcaaac cgttacgat aaaacaaaa 
atatttagg tttggacgt tttcgatca attgcgact 
cgaaaccaa cattcaggg ggaaatccc gactttacc 
gttcgactc aaaaatcga aatcgatga gtatcagga 
acagtacac tttgacgag tcccggagt ggttcaaat 
gtcatcgac tcgcttttt caaatactt ggacgaaaa 
caaagaaga gaccaccgt ttcgtacga cattgaaca 
agtctacac ttttgatta ctgcaagca cgagaatac 
ctcactcgg cgagagctg gaagctcaa agtcggcaa 
aaacttcat cagttacga gagcaaaga acagatcat 
ccgtttcgg cttaactaa caagatcat gcctttggg 
ggagatcga cccttgcca ggagggccg ctcccagtg 
cgccccttt cagtgcctg cgtcgtgga cggcgacag 
cttccggtg cgtttgcaa cccaggttt ccagcaaat 
gtttgccga aaacgagac agtctgcgc cgacattga 
cgaatgcca gaccgggct ccacagttg cgaccagaa 
cgcagcctg tgtgaacca ggtcgggtc gtactcctg 
ctcttgcaa tccgggctt caccggaaa cgggcaagt 
gtgcgagaa cgaattctc gtgccataa cgtcatttg 
ccccccgaa tgccgagtg cgtggaggg cgacaacgt 
ggcaatgtg ccgctgcat gcccggatt caccggaga 
cgggcaagt ttgtagccc gctggtcga ccggagttg 
ccacacggc caacaactg cgacccctt cgccgtctg 
caccatcga ccccaagac gaacagcta ctactgtgt 
gtgtttgcc ggggtacga aggggacgg ctacagttg 
tagcaaggt ggaagtgca aaacgtgac ggaagcggc 
ggtcgagaa ggagatcca gaggtgcgt cctgggagt 
gtgttggtg ccccgaagg gtacacttt tgagaaagg 
gaccacgta ttgcatccc caaagagga gacgactgt 
tctaccgcc gactacgcc acttgcgga taatgtcac 
gtgtgatat tttgaataa ttgtaacgc caacgctca 
gtgtattta caattcgga aaatgaaac tttctcgtg 
tatctgtaa cgaaggtta cgacgggga tggttacac 
ctgcgatgc tgtaaccgt gtcgtgtgc gcaggaata 
taactgtgg gcccaatgc aaattgtta ctacgacga 
aactgtcgg gaagtcgaa atgtgtgtg caacccggg 
gtatttcgg ggacggttt caactgcac aatcacagc 
catttgcac ctccaatgc cgactgcac ccaaaccga 
ggagtgcct tctgtccag ctcacagcg ctacgaatg 
cgtctgtaa agagggcta cgtccggga ttcccaaaa 
ccagtgcat taaacccag cacttgtgg cggcggact 
ttgcgtcga aaacgccga gtgtctcta cgacgacac 
ctaccagct ccattattg ctcctgcaa gagtggtta 
catggggga cggcataac cgaatgtaa accgcgccc 
catcggttg taacattga aaataaatg cggcctcca 
cgccacctg cgagtacga cccctccac ctccttgta 
cgagtgtca gtgtgaacg tggttacta cggcgatgg 
ttacgtgtg ttacaccga aatcaactg tcacatcga 
gccaacttt gtgcgaccc acaagccac gtgtgttac 
agactcgaa ccggaggta cgtctgtca gtgtaacac 
tggctacgt ggggaacgg aaccatctg tcggaagaa 
tcccaccca cgaaggcaa cttcctgtt actcaacca 
gggaatgtc aacactaaa aatcccatt cgaacccac 
gaagcaaaa ttcaggaaa acccatcca ggtcaagta 
cttccaaac tgctgtggg cctggacat tgactgtta 
cgaaggacg ggtctattg gggtgacat cagcgggag 
ggcgatcag gagctcctc ctacaacgg gagtaacaa 
gagcgactt tatcgtgga agggattgg gtcaccgga 
aggactagc agttgattg ggtttcaag aaacattta 
ttggaccga ttcgaccaa agatacagt cgaagtcgc 
gagtattga gacgaggcg gaggcgagt ccttttcga 
tttcaattt agtcaaccc gagaggaat cgccgtgca 
tccgcaacg aggcaaaat cttttggac cgattggga 
ccgcaagaa ccccaagat cgagtgggc gaacggcga 
cggctccgg ccgcggcat tttcctgca aggggaagc 
cgtttccct tcccaactc tctcaccat tgactacga 
caccgaaca attgtgtta cgcggacgc cggaacgaa 
aaaaatcga atgtgtcga tatcgacag caaaatcag 
gcgcacaat tgccaccaa ctgcacata tccattcgg 
gataacagt caccgataa acacatcta ttggtcgga 
ctggatcac gaagaaaat tgaacgggc ggagaaaaa 
cacgctgac acgtttgcc gcccctgca ggttcctct 
cggcggcag tgggaacaa gttgtttgg gttggtggc 
agtgcctca gagttgtcc ccagttgac aaacgtgtg 
ccaatatta caaggacca atgccctgt cgatcacat 
ttgtctccc gaacggcgc aggaagtcg gacttgtgt 
ctgcacttt caaagccga cagttctga aacaccttc 
ttgcaaatt ataattacg attaattaa gtttgtagt 
ttaggtttt ctacaataa aacactttt ctaatt
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