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Gene information
General information
TC008043 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4253547..4258119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008043
    iBeetle screen iB_01306

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature spotted - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: dorsal cuticle seems to be slightly invaginated, causing loss of some bristles.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: dorsal cuticle seems to be slightly invaginated, causing loss of some bristles.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008043 RA
      MKLLRLFSC CLFFFTGAT ALPVGLLYS HNVQEARKL 
      PQENDVSSP EIKLKVPVV FYGTKFNSI FVNSNGFVS 
      FQTEIPNFF NIEFPLDYP LIAPFYTNV DTRSAGTIS 
      YYETSDSAL IHRATENVR EYFSDQENF QAKSLFIVT 
      WFRVGYFNR GSDKTNTYQ LVIITDGAS SFVELLYPK 
      EGIQWIQGT GDESGLPDA RAQAGFISA EGKMYTLPG 
      SGTEQVRNL EVWSNIDLP GQFMYRVDG NDVIGPDLI 
      TDDLKSDEN PQTCAEATT YCHVQANCV DYEEGFCCQ 
      CKKQYYGNG RFCVKKDVP LRVNGKING KINGERLEN 
      LDLQSYIVM VDGRAYTAI SKIPESIGF DIQSLQILG 
      GVIGYIFAK PIRNAQNGY QFTGGVFNH TAILKFLNT 
      SQTVTIKQK YLGLDVFDQ LRLETNIQG EIPTLPFDS 
      KIEIDEYQE QYTLTSPGV VQMSSTRFF KYLDENKEE 
      TTVSYDIEQ VYTFDYCKH ENTSLGESW KLKVGKNFI 
      SYESKEQII RFGLTNKIM PLGEIDPCQ EGRSQCAPF 
      SACVVDGDS FRCVCNPGF QQMFAENET VCADIDECQ 
      TGLHSCDQN AACVNQVGS YSCSCNPGF TGNGQVCEN 
      EFSCHNVIC PPNAECVEG DNVAMCRCM PGFTGDGQV 
      CSPLVDRSC HTANNCDPF AVCTIDPKT NSYYCVCLP 
      GYEGDGYSC SKVEVQNVT EAAVEKEIQ RCVLGVCWC 
      PEGYTFEKG TTYCIPKEE TTVLPPTTP LADNVTCDI 
      LNNCNANAQ CIYNSENET FSCICNEGY DGDGYTCDA 
      VTVSCAQEY NCGPNANCY YDETVGKSK CVCNPGYFG 
      DGFNCTITA ICTSNADCT QTEECLLSS SQRYECVCK 
      EGYVRDSQN QCIKPSTCG GGLCVENAE CLYDDTYQL 
      HYCSCKSGY MGDGITECK PRPIGCNIE NKCGLHATC 
      EYDPSTSLY ECQCERGYY GDGYVCYTE INCHIEPTL 
      CDPQATCVT DSNRRYVCQ CNTGYVGNG TICRKNPTH 
      EGNFLLLNQ GMSTLKIPF EPTKQNSGK PIQVKYFQT 
      AVGLDIDCY EGRVYWGDI SGRAIRSSS YNGSNKSDF 
      IVEGIGSPE GLAVDWVSR NIYWTDSTK DTVEVASIE 
      TRRRRVLFD FNLVNPRGI AVHPQRGKI FWTDWDRKN 
      PKIEWANGD GSGRGIFLQ GEAVSLPNS LTIDYDTEQ 
      LCYADAGTK KIECVDIDS KIRRTIATN CTYPFGITV 
      TDKHIYWSD WITKKIERA EKNTLTRLP PLQVPLGGS 
      GNKLFGLVA VPQSCPQLT NVCQYYKDQ CPVDHICLP 
      NGAGSRTCV CTFKADSSE TPSCKL
    CDS sequence
    • >TC008043 RA
      atgaagttg cttcgactt ttctcttgt tgcctattt 
      tttttcacg ggcgcgacc gccttacca gtcggactc 
      ttatacagt cataatgtg caagaagcc cgcaaattg 
      ccacaggaa aatgacgtc tccagcccg gaaatcaag 
      ctcaaagtg cccgtggtt ttctacgga acgaaattc 
      aactcgatt tttgtgaac tcaaacggc ttcgtctcc 
      ttccaaacc gaaatcccc aacttcttc aacatcgag 
      ttccccctc gactacccc ctcatcgcc cccttttac 
      acgaacgtg gacaccagg agcgctggg acgatttcc 
      tactacgaa acctccgac tcggccctc attcaccgc 
      gccacagaa aacgtccgt gaatacttc tccgaccag 
      gaaaatttc caggccaaa agtctattt atcgtgact 
      tggttccgt gtcggttat ttcaacaga ggcagcgac 
      aaaaccaac acttatcaa ctggtgatc atcaccgac 
      ggcgccagc tcattcgtt gaactcctc taccccaaa 
      gagggcatc cagtggatc caaggcacc ggggacgag 
      tccgggctc ccggacgcc cgcgcccaa gccggattc 
      atctccgcc gagggcaaa atgtacacg cttccgggc 
      tccggcacg gagcaagtc cggaatttg gaagtgtgg 
      tccaatatt gacctcccg gggcagttc atgtacagg 
      gttgatggg aatgacgtg atcgggccc gatttgatc 
      accgatgat ttgaaaagc gacgagaat ccgcaaacg 
      tgtgccgaa gctacgact tattgtcac gtccaagcc 
      aactgtgtg gactacgag gaggggttc tgctgccag 
      tgcaagaaa cagtactac gggaatggg cggttttgt 
      gtaaagaaa gacgttccg ctgagagtt aatgggaaa 
      atcaacggg aaaattaac ggcgaacga ctggaaaat 
      ttggacttg cagtcgtac attgtcatg gtcgatggc 
      cgggcctac acggctatt agcaaaatc cccgaatct 
      ataggtttt gacatccaa tccttgcaa attctgggt 
      ggggtaatt ggttatatt ttcgccaaa cccatcaga 
      aacgcccaa aatgggtac caattcacg ggaggtgtt 
      ttcaaccac actgccatt ctcaaattc ttaaacacc 
      agtcaaacc gttacgata aaacaaaaa tatttaggt 
      ttggacgtt ttcgatcaa ttgcgactc gaaaccaac 
      attcagggg gaaatcccg actttaccg ttcgactca 
      aaaatcgaa atcgatgag tatcaggaa cagtacact 
      ttgacgagt cccggagtg gttcaaatg tcatcgact 
      cgctttttc aaatacttg gacgaaaac aaagaagag 
      accaccgtt tcgtacgac attgaacaa gtctacact 
      tttgattac tgcaagcac gagaatacc tcactcggc 
      gagagctgg aagctcaaa gtcggcaaa aacttcatc 
      agttacgag agcaaagaa cagatcatc cgtttcggc 
      ttaactaac aagatcatg cctttgggg gagatcgac 
      ccttgccag gagggccgc tcccagtgc gcccctttc 
      agtgcctgc gtcgtggac ggcgacagc ttccggtgc 
      gtttgcaac ccaggtttc cagcaaatg tttgccgaa 
      aacgagaca gtctgcgcc gacattgac gaatgccag 
      accgggctc cacagttgc gaccagaac gcagcctgt 
      gtgaaccag gtcgggtcg tactcctgc tcttgcaat 
      ccgggcttc accggaaac gggcaagtg tgcgagaac 
      gaattctcg tgccataac gtcatttgc cccccgaat 
      gccgagtgc gtggagggc gacaacgtg gcaatgtgc 
      cgctgcatg cccggattc accggagac gggcaagtt 
      tgtagcccg ctggtcgac cggagttgc cacacggcc 
      aacaactgc gaccccttc gccgtctgc accatcgac 
      cccaagacg aacagctac tactgtgtg tgtttgccg 
      gggtacgaa ggggacggc tacagttgt agcaaggtg 
      gaagtgcaa aacgtgacg gaagcggcg gtcgagaag 
      gagatccag aggtgcgtc ctgggagtg tgttggtgc 
      cccgaaggg tacactttt gagaaaggg accacgtat 
      tgcatcccc aaagaggag acgactgtt ctaccgccg 
      actacgcca cttgcggat aatgtcacg tgtgatatt 
      ttgaataat tgtaacgcc aacgctcag tgtatttac 
      aattcggaa aatgaaact ttctcgtgt atctgtaac 
      gaaggttac gacggggat ggttacacc tgcgatgct 
      gtaaccgtg tcgtgtgcg caggaatat aactgtggg 
      cccaatgca aattgttac tacgacgaa actgtcggg 
      aagtcgaaa tgtgtgtgc aacccgggg tatttcggg 
      gacggtttc aactgcaca atcacagcc atttgcacc 
      tccaatgcc gactgcacc caaaccgag gagtgcctt 
      ctgtccagc tcacagcgc tacgaatgc gtctgtaaa 
      gagggctac gtccgggat tcccaaaac cagtgcatt 
      aaacccagc acttgtggc ggcggactt tgcgtcgaa 
      aacgccgag tgtctctac gacgacacc taccagctc 
      cattattgc tcctgcaag agtggttac atgggggac 
      ggcataacc gaatgtaaa ccgcgcccc atcggttgt 
      aacattgaa aataaatgc ggcctccac gccacctgc 
      gagtacgac ccctccacc tccttgtac gagtgtcag 
      tgtgaacgt ggttactac ggcgatggt tacgtgtgt 
      tacaccgaa atcaactgt cacatcgag ccaactttg 
      tgcgaccca caagccacg tgtgttaca gactcgaac 
      cggaggtac gtctgtcag tgtaacact ggctacgtg 
      gggaacgga accatctgt cggaagaat cccacccac 
      gaaggcaac ttcctgtta ctcaaccag ggaatgtca 
      acactaaaa atcccattc gaacccacg aagcaaaat 
      tcaggaaaa cccatccag gtcaagtac ttccaaact 
      gctgtgggc ctggacatt gactgttac gaaggacgg 
      gtctattgg ggtgacatc agcgggagg gcgatcagg 
      agctcctcc tacaacggg agtaacaag agcgacttt 
      atcgtggaa gggattggg tcaccggaa ggactagca 
      gttgattgg gtttcaaga aacatttat tggaccgat 
      tcgaccaaa gatacagtc gaagtcgcg agtattgag 
      acgaggcgg aggcgagtc cttttcgat ttcaattta 
      gtcaacccg agaggaatc gccgtgcat ccgcaacga 
      ggcaaaatc ttttggacc gattgggac cgcaagaac 
      cccaagatc gagtgggcg aacggcgac ggctccggc 
      cgcggcatt ttcctgcaa ggggaagcc gtttccctt 
      cccaactct ctcaccatt gactacgac accgaacaa 
      ttgtgttac gcggacgcc ggaacgaaa aaaatcgaa 
      tgtgtcgat atcgacagc aaaatcagg cgcacaatt 
      gccaccaac tgcacatat ccattcggg ataacagtc 
      accgataaa cacatctat tggtcggac tggatcacg 
      aagaaaatt gaacgggcg gagaaaaac acgctgaca 
      cgtttgccg cccctgcag gttcctctc ggcggcagt 
      gggaacaag ttgtttggg ttggtggca gtgcctcag 
      agttgtccc cagttgaca aacgtgtgc caatattac 
      aaggaccaa tgccctgtc gatcacatt tgtctcccg 
      aacggcgca ggaagtcgg acttgtgtc tgcactttc 
      aaagccgac agttctgaa acaccttct tgcaaatta 
      taa
    mRNA sequence
    • >TC008043 RA
      atacgagcg attatttta agtcagtta tagtcagtg 
      gctttcgaa aatgaagtt gcttcgact tttctcttg 
      ttgcctatt ttttttcac gggcgcgac cgccttacc 
      agtcggact cttatacag tcataatgt gcaagaagc 
      ccgcaaatt gccacagga aaatgacgt ctccagccc 
      ggaaatcaa gctcaaagt gcccgtggt tttctacgg 
      aacgaaatt caactcgat ttttgtgaa ctcaaacgg 
      cttcgtctc cttccaaac cgaaatccc caacttctt 
      caacatcga gttccccct cgactaccc cctcatcgc 
      cccctttta cacgaacgt ggacaccag gagcgctgg 
      gacgatttc ctactacga aacctccga ctcggccct 
      cattcaccg cgccacaga aaacgtccg tgaatactt 
      ctccgacca ggaaaattt ccaggccaa aagtctatt 
      tatcgtgac ttggttccg tgtcggtta tttcaacag 
      aggcagcga caaaaccaa cacttatca actggtgat 
      catcaccga cggcgccag ctcattcgt tgaactcct 
      ctaccccaa agagggcat ccagtggat ccaaggcac 
      cggggacga gtccgggct cccggacgc ccgcgccca 
      agccggatt catctccgc cgagggcaa aatgtacac 
      gcttccggg ctccggcac ggagcaagt ccggaattt 
      ggaagtgtg gtccaatat tgacctccc ggggcagtt 
      catgtacag ggttgatgg gaatgacgt gatcgggcc 
      cgatttgat caccgatga tttgaaaag cgacgagaa 
      tccgcaaac gtgtgccga agctacgac ttattgtca 
      cgtccaagc caactgtgt ggactacga ggaggggtt 
      ctgctgcca gtgcaagaa acagtacta cgggaatgg 
      gcggttttg tgtaaagaa agacgttcc gctgagagt 
      taatgggaa aatcaacgg gaaaattaa cggcgaacg 
      actggaaaa tttggactt gcagtcgta cattgtcat 
      ggtcgatgg ccgggccta cacggctat tagcaaaat 
      ccccgaatc tataggttt tgacatcca atccttgca 
      aattctggg tggggtaat tggttatat tttcgccaa 
      acccatcag aaacgccca aaatgggta ccaattcac 
      gggaggtgt tttcaacca cactgccat tctcaaatt 
      cttaaacac cagtcaaac cgttacgat aaaacaaaa 
      atatttagg tttggacgt tttcgatca attgcgact 
      cgaaaccaa cattcaggg ggaaatccc gactttacc 
      gttcgactc aaaaatcga aatcgatga gtatcagga 
      acagtacac tttgacgag tcccggagt ggttcaaat 
      gtcatcgac tcgcttttt caaatactt ggacgaaaa 
      caaagaaga gaccaccgt ttcgtacga cattgaaca 
      agtctacac ttttgatta ctgcaagca cgagaatac 
      ctcactcgg cgagagctg gaagctcaa agtcggcaa 
      aaacttcat cagttacga gagcaaaga acagatcat 
      ccgtttcgg cttaactaa caagatcat gcctttggg 
      ggagatcga cccttgcca ggagggccg ctcccagtg 
      cgccccttt cagtgcctg cgtcgtgga cggcgacag 
      cttccggtg cgtttgcaa cccaggttt ccagcaaat 
      gtttgccga aaacgagac agtctgcgc cgacattga 
      cgaatgcca gaccgggct ccacagttg cgaccagaa 
      cgcagcctg tgtgaacca ggtcgggtc gtactcctg 
      ctcttgcaa tccgggctt caccggaaa cgggcaagt 
      gtgcgagaa cgaattctc gtgccataa cgtcatttg 
      ccccccgaa tgccgagtg cgtggaggg cgacaacgt 
      ggcaatgtg ccgctgcat gcccggatt caccggaga 
      cgggcaagt ttgtagccc gctggtcga ccggagttg 
      ccacacggc caacaactg cgacccctt cgccgtctg 
      caccatcga ccccaagac gaacagcta ctactgtgt 
      gtgtttgcc ggggtacga aggggacgg ctacagttg 
      tagcaaggt ggaagtgca aaacgtgac ggaagcggc 
      ggtcgagaa ggagatcca gaggtgcgt cctgggagt 
      gtgttggtg ccccgaagg gtacacttt tgagaaagg 
      gaccacgta ttgcatccc caaagagga gacgactgt 
      tctaccgcc gactacgcc acttgcgga taatgtcac 
      gtgtgatat tttgaataa ttgtaacgc caacgctca 
      gtgtattta caattcgga aaatgaaac tttctcgtg 
      tatctgtaa cgaaggtta cgacgggga tggttacac 
      ctgcgatgc tgtaaccgt gtcgtgtgc gcaggaata 
      taactgtgg gcccaatgc aaattgtta ctacgacga 
      aactgtcgg gaagtcgaa atgtgtgtg caacccggg 
      gtatttcgg ggacggttt caactgcac aatcacagc 
      catttgcac ctccaatgc cgactgcac ccaaaccga 
      ggagtgcct tctgtccag ctcacagcg ctacgaatg 
      cgtctgtaa agagggcta cgtccggga ttcccaaaa 
      ccagtgcat taaacccag cacttgtgg cggcggact 
      ttgcgtcga aaacgccga gtgtctcta cgacgacac 
      ctaccagct ccattattg ctcctgcaa gagtggtta 
      catggggga cggcataac cgaatgtaa accgcgccc 
      catcggttg taacattga aaataaatg cggcctcca 
      cgccacctg cgagtacga cccctccac ctccttgta 
      cgagtgtca gtgtgaacg tggttacta cggcgatgg 
      ttacgtgtg ttacaccga aatcaactg tcacatcga 
      gccaacttt gtgcgaccc acaagccac gtgtgttac 
      agactcgaa ccggaggta cgtctgtca gtgtaacac 
      tggctacgt ggggaacgg aaccatctg tcggaagaa 
      tcccaccca cgaaggcaa cttcctgtt actcaacca 
      gggaatgtc aacactaaa aatcccatt cgaacccac 
      gaagcaaaa ttcaggaaa acccatcca ggtcaagta 
      cttccaaac tgctgtggg cctggacat tgactgtta 
      cgaaggacg ggtctattg gggtgacat cagcgggag 
      ggcgatcag gagctcctc ctacaacgg gagtaacaa 
      gagcgactt tatcgtgga agggattgg gtcaccgga 
      aggactagc agttgattg ggtttcaag aaacattta 
      ttggaccga ttcgaccaa agatacagt cgaagtcgc 
      gagtattga gacgaggcg gaggcgagt ccttttcga 
      tttcaattt agtcaaccc gagaggaat cgccgtgca 
      tccgcaacg aggcaaaat cttttggac cgattggga 
      ccgcaagaa ccccaagat cgagtgggc gaacggcga 
      cggctccgg ccgcggcat tttcctgca aggggaagc 
      cgtttccct tcccaactc tctcaccat tgactacga 
      caccgaaca attgtgtta cgcggacgc cggaacgaa 
      aaaaatcga atgtgtcga tatcgacag caaaatcag 
      gcgcacaat tgccaccaa ctgcacata tccattcgg 
      gataacagt caccgataa acacatcta ttggtcgga 
      ctggatcac gaagaaaat tgaacgggc ggagaaaaa 
      cacgctgac acgtttgcc gcccctgca ggttcctct 
      cggcggcag tgggaacaa gttgtttgg gttggtggc 
      agtgcctca gagttgtcc ccagttgac aaacgtgtg 
      ccaatatta caaggacca atgccctgt cgatcacat 
      ttgtctccc gaacggcgc aggaagtcg gacttgtgt 
      ctgcacttt caaagccga cagttctga aacaccttc 
      ttgcaaatt ataattacg attaattaa gtttgtagt 
      ttaggtttt ctacaataa aacactttt ctaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY