Status
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Gene information
General information
TC032801
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008027
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4067233..4071547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032801
    iBeetle screen iB_01305

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (13 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032801 RA
      MTKKFEFDW HVPVPEPLL TGCVVDRWS EEPNEIEWK 
      ALFRVDEYG FFIYWKSEG KEGDVIELC QVSDVRAGG 
      LPKDLKLFS QLESRHGPD LEKRSLTIC SGTDYININ 
      YQHIVCPNA ETAKVWLDG LRKITHNVK ANNFCPMTC 
      LKKHWMRLG FLTDAKGKV PVKVVARTF ASGKSTEKL 
      VYQCLADLD LPNGKNAVM EPEQFTFEK FYTLYHKIC 
      PRNDIEELF RSITQGKAD YMNLEQFIN FLNEKQRDP 
      RLNEILYPH YDAKRASEI IATYEQDEE AKAANRLTK 
      DGLIRYLMS DENAPVFLD RLDIYMDMD QPLCAYYIN 
      SSHNTYLSG RQFGGRSSV EMYRQVLLA GCRCVELDC 
      WDGRGEDEE PIITHGRAM CTDILFKDV IYAIRDTAF 
      VTSDYPVIL SFENHCCKS QQYKLAKYC DEILGDLLL 
      KEPLPDYPL EPGLPLPPP SCLKRKILI KNKRLKPEV 
      EKQELELFR QGEFVIEDE EKEDASASA DRPIPPDIS 
      AETPAPDGG AGDGDIAAP PIIHTGSTT NVHPWLSSM 
      VNYAQPVKF TSFDYAEEK NIHYNMSSF SEPVGLNLL 
      KTQAIEFVN YNKRQMSRI YPSGARANS SNYMPQVFW 
      NAGCQMVSL NFQTSDLPM QLNQGKFEY NGNCGYLLK 
      PDFMRRPDR TFDPFAESP VDGVIAAQC SVQVIAGQF 
      LSDKKVGTY VEVDMYGLP TDTIRREFR TRMVPANGL 
      NPVYNEEPF LFRKVVLPD LAVLRFGVY DENNRLLGQ 
      RILPLDGLQ AGYRHISLR TEANFPMSL PMLFCNIEL 
      KIYVPDGFE DFMAALSDP RGFSGARQK QNEQMSSMG 
      IEVTDKGTE KKEKEKEEK KEEPLVFEP ITIESIKQD 
      KAFQKTTKK QQKEYDTLK KKQTKEKLA VQKSQCSAI 
      DKLIKGKNK TDLVNDPGV RKLVTEQTL EWSALMERH 
      RKQEWEFLR NQLDEQRDA LKRHMVILQ AAQMKQLEA 
      KHDREFKEM IGQQAKVSV EIAKEVAND KNLKTKRDK 
      ERRLKEKQQ NNTKRFLEE RKTASNRQA KEKEKLKIK 
      HEKQLEMLS HDIQKLIDM YKNEELEYE MSSKSEFFA
    CDS sequence
    • >TC032801 RA
      atgacgaaa aaattcgag ttcgattgg catgtcccc 
      gtgccggag cccctgctc acgggctgc gtcgtggac 
      aggtggtcg gaggagccc aacgaaatc gaatggaaa 
      gcccttttc cgggtggac gagtacgga tttttcatc 
      tattggaag agcgaggga aaggagggc gacgtcatc 
      gaactgtgc caagtgagc gatgtgagg gccggtggg 
      ctgcccaaa gacctcaaa ctgttcagc cagctcgaa 
      agccgacac gggcccgat cttgagaag agatcgctg 
      accatctgt agcgggacc gactatatc aacattaat 
      taccagcac atcgtgtgc cccaatgcc gagacggca 
      aaggtttgg ttggacggt ttgcgaaag ataacacat 
      aacgtgaaa gccaataac ttctgtccg atgacgtgt 
      ttgaaaaaa cattggatg cgactcggt tttctgacc 
      gatgccaag ggtaaagtg cccgtcaaa gtcgtagcg 
      cgtactttc gcctccggc aaaagtaca gaaaagttg 
      gtttatcag tgtttggcc gacttggat ctaccgaac 
      ggcaaaaac gcagtcatg gagcccgag caattcacc 
      ttcgagaaa ttctacact ttgtatcac aaaatctgc 
      cctcggaac gacatcgag gagctcttc cgcagcatt 
      acgcaaggc aaagccgac tatatgaat ctcgagcag 
      tttattaat ttcctcaac gagaaacag cgagacccg 
      cgtttgaac gaaattttg tacccacat tatgacgcg 
      aaacgagcg tccgaaata attgccact tatgaacaa 
      gacgaggag gcgaaggca gcaaatagg ttgacaaaa 
      gacggtctg attaggtat ttaatgtcg gacgaaaac 
      gctccggtg tttttggac cgtttggac atctacatg 
      gatatggac cagcctcta tgcgcctac tatataaat 
      tctagtcac aatacgtat ttaagtggc cgccagttt 
      ggcggtagg tcttccgtt gaaatgtac agacaagta 
      ttgctggcg gggtgtcgc tgcgtcgaa ctcgactgt 
      tgggacggc cgaggcgaa gacgaggag ccgatcata 
      acccacgga cgcgccatg tgcacggac atcctcttc 
      aaagacgta atctacgca atccgagac accgctttc 
      gtaacatcc gactatccc gtaatcctc tcctttgag 
      aaccactgc tgcaaaagc caacagtac aagctcgcg 
      aaatattgc gacgaaatc ttgggcgat ttgctcctc 
      aaagaacca cttcctgac tatcccctt gaaccaggc 
      ctcccgtta ccgccacct tcatgcctc aagcgtaaa 
      attttaatc aagaataag agactcaaa ccagaggtg 
      gagaaacaa gaactggag ttgttcaga cagggggag 
      ttcgttatc gaagatgag gaaaaagag gatgcgagt 
      gcgtccgca gaccggcct ataccaccg gatatttcg 
      gctgaaaca ccggcgccc gatgggggg gctggtgac 
      ggggacatc gcagcgccg cctataata cacacgggg 
      tcgactact aatgtgcat ccgtggttg agttcgatg 
      gttaattat gcccaacct gtcaaattc actagtttt 
      gattatgcg gaagagaaa aacatacat tacaacatg 
      tcaagtttt tcggaacct gtcggcttg aatttgctc 
      aagactcag gcgattgag ttcgtcaat tacaacaaa 
      cgccaaatg tcgcgcatt tacccgagc ggcgctcgg 
      gccaactcg tcgaactat atgccgcag gttttctgg 
      aatgctggt tgtcaaatg gtctcttta aatttccaa 
      acttccgat cttcccatg caactcaat caaggcaaa 
      tttgagtac aacggcaac tgtggttat ttattaaaa 
      ccggatttt atgcgacgt cccgaccgg actttcgat 
      ccgttcgct gaaagtcca gtagatggc gttattgcg 
      gcccaatgt tcggtccag gtgatcgct gggcagttc 
      ttgtccgac aaaaaggtt ggtacctac gtcgaggtc 
      gatatgtac ggcttgccg accgatacg atccgacgc 
      gagttccga actcggatg gttccggcc aatggtttg 
      aatccggtg tataacgaa gaaccgttt cttttccgg 
      aaagtggtt cttccggac ttggctgtg ctacgattt 
      ggggtttac gacgagaat aacagactt ttgggtcag 
      agaattttg ccattggat ggtctccaa gccggctat 
      cggcacatt tccttaaga actgaagcc aatttcccc 
      atgtcgctt ccaatgttg ttttgtaac attgagtta 
      aagatctac gtcccggat ggttttgag gacttcatg 
      gccgcactg agtgaccct cgggggttc agcggagcc 
      cgacaaaag caaaacgaa caaatgtca tcgatggga 
      atcgaagtg accgataaa ggaacggaa aagaaggaa 
      aaagagaaa gaagagaag aaggaagaa ccgcttgtt 
      ttcgaacca atcaccata gaatcaata aaacaagac 
      aaagccttc cagaaaacc accaaaaaa cagcaaaaa 
      gaatatgac acgcttaaa aagaagcaa acgaaggag 
      aagctcgcc gtgcaaaag tcgcaatgt tcggccatc 
      gataagtta atcaagggc aaaaacaaa actgatctg 
      gttaatgac ccaggggtg cgcaagctc gtaaccgag 
      caaactctc gagtggtcg gccctgatg gagcgccac 
      cgtaaacaa gagtgggaa tttttgcgt aatcagttg 
      gacgaacaa cgtgacgcg ttaaagcgt cacatggtg 
      attttacaa gctgctcaa atgaaacaa ttagaggcc 
      aaacatgac agggaattt aaagagatg atcggtcaa 
      caagcgaaa gtttcggtc gaaattgcc aaagaagtt 
      gccaatgat aaaaacttg aaaactaag cgagacaag 
      gagcggaga ctgaaggag aagcagcaa aacaatacg 
      aaacggttt ctggaagag agaaaaact gcatcgaat 
      cgacaagcc aaagagaag gagaagttg aagatcaag 
      cacgagaag cagctggaa atgctgtcg catgacatc 
      cagaagctg atcgacatg tacaagaat gaggagttg 
      gagtatgag atgtcgagc aaaagcgag ttttttgct 
      taa
    mRNA sequence
    • >TC032801 RA
      tgtcaaata aataaacat gtcccttgc acaaagatg 
      agtttttcg tgctcgccg cttaacgtt tttactttt 
      cgttgaact agccatgaa tatcgtcta attgtcttg 
      catctagcc gatcatgac gaaaaaatt cgagttcga 
      ttggcatgt ccccgtgcc ggagcccct gctcacggg 
      ctgcgtcgt ggacaggtg gtcggagga gcccaacga 
      aatcgaatg gaaagccct tttccgggt ggacgagta 
      cggattttt catctattg gaagagcga gggaaagga 
      gggcgacgt catcgaact gtgccaagt gagcgatgt 
      gagggccgg tgggctgcc caaagacct caaactgtt 
      cagccagct cgaaagccg acacgggcc cgatcttga 
      gaagagatc gctgaccat ctgtagcgg gaccgacta 
      tatcaacat taattacca gcacatcgt gtgccccaa 
      tgccgagac ggcaaaggt ttggttgga cggtttgcg 
      aaagataac acataacgt gaaagccaa taacttctg 
      tccgatgac gtgtttgaa aaaacattg gatgcgact 
      cggttttct gaccgatgc caagggtaa agtgcccgt 
      caaagtcgt agcgcgtac tttcgcctc cggcaaaag 
      tacagaaaa gttggttta tcagtgttt ggccgactt 
      ggatctacc gaacggcaa aaacgcagt catggagcc 
      cgagcaatt caccttcga gaaattcta cactttgta 
      tcacaaaat ctgccctcg gaacgacat cgaggagct 
      cttccgcag cattacgca aggcaaagc cgactatat 
      gaatctcga gcagtttat taatttcct caacgagaa 
      acagcgaga cccgcgttt gaacgaaat tttgtaccc 
      acattatga cgcgaaacg agcgtccga aataattgc 
      cacttatga acaagacga ggaggcgaa ggcagcaaa 
      taggttgac aaaagacgg tctgattag gtatttaat 
      gtcggacga aaacgctcc ggtgttttt ggaccgttt 
      ggacatcta catggatat ggaccagcc tctatgcgc 
      ctactatat aaattctag tcacaatac gtatttaag 
      tggccgcca gtttggcgg taggtcttc cgttgaaat 
      gtacagaca agtattgct ggcggggtg tcgctgcgt 
      cgaactcga ctgttggga cggccgagg cgaagacga 
      ggagccgat cataaccca cggacgcgc catgtgcac 
      ggacatcct cttcaaaga cgtaatcta cgcaatccg 
      agacaccgc tttcgtaac atccgacta tcccgtaat 
      cctctcctt tgagaacca ctgctgcaa aagccaaca 
      gtacaagct cgcgaaata ttgcgacga aatcttggg 
      cgatttgct cctcaaaga accacttcc tgactatcc 
      ccttgaacc aggcctccc gttaccgcc accttcatg 
      cctcaagcg taaaatttt aatcaagaa taagagact 
      caaaccaga ggtggagaa acaagaact ggagttgtt 
      cagacaggg ggagttcgt tatcgaaga tgaggaaaa 
      agaggatgc gagtgcgtc cgcagaccg gcctatacc 
      accggatat ttcggctga aacaccggc gcccgatgg 
      gggggctgg tgacgggga catcgcagc gccgcctat 
      aatacacac ggggtcgac tactaatgt gcatccgtg 
      gttgagttc gatggttaa ttatgccca acctgtcaa 
      attcactag ttttgatta tgcggaaga gaaaaacat 
      acattacaa catgtcaag tttttcgga acctgtcgg 
      cttgaattt gctcaagac tcaggcgat tgagttcgt 
      caattacaa caaacgcca aatgtcgcg catttaccc 
      gagcggcgc tcgggccaa ctcgtcgaa ctatatgcc 
      gcaggtttt ctggaatgc tggttgtca aatggtctc 
      tttaaattt ccaaacttc cgatcttcc catgcaact 
      caatcaagg caaatttga gtacaacgg caactgtgg 
      ttatttatt aaaaccgga ttttatgcg acgtcccga 
      ccggacttt cgatccgtt cgctgaaag tccagtaga 
      tggcgttat tgcggccca atgttcggt ccaggtgat 
      cgctgggca gttcttgtc cgacaaaaa ggttggtac 
      ctacgtcga ggtcgatat gtacggctt gccgaccga 
      tacgatccg acgcgagtt ccgaactcg gatggttcc 
      ggccaatgg tttgaatcc ggtgtataa cgaagaacc 
      gtttctttt ccggaaagt ggttcttcc ggacttggc 
      tgtgctacg atttggggt ttacgacga gaataacag 
      acttttggg tcagagaat tttgccatt ggatggtct 
      ccaagccgg ctatcggca catttcctt aagaactga 
      agccaattt ccccatgtc gcttccaat gttgttttg 
      taacattga gttaaagat ctacgtccc ggatggttt 
      tgaggactt catggccgc actgagtga ccctcgggg 
      gttcagcgg agcccgaca aaagcaaaa cgaacaaat 
      gtcatcgat gggaatcga agtgaccga taaaggaac 
      ggaaaagaa ggaaaaaga gaaagaaga gaagaagga 
      agaaccgct tgttttcga accaatcac catagaatc 
      aataaaaca agacaaagc cttccagaa aaccaccaa 
      aaaacagca aaaagaata tgacacgct taaaaagaa 
      gcaaacgaa ggagaagct cgccgtgca aaagtcgca 
      atgttcggc catcgataa gttaatcaa gggcaaaaa 
      caaaactga tctggttaa tgacccagg ggtgcgcaa 
      gctcgtaac cgagcaaac tctcgagtg gtcggccct 
      gatggagcg ccaccgtaa acaagagtg ggaattttt 
      gcgtaatca gttggacga acaacgtga cgcgttaaa 
      gcgtcacat ggtgatttt acaagctgc tcaaatgaa 
      acaattaga ggccaaaca tgacaggga atttaaaga 
      gatgatcgg tcaacaagc gaaagtttc ggtcgaaat 
      tgccaaaga agttgccaa tgataaaaa cttgaaaac 
      taagcgaga caaggagcg gagactgaa ggagaagca 
      gcaaaacaa tacgaaacg gtttctgga agagagaaa 
      aactgcatc gaatcgaca agccaaaga gaaggagaa 
      gttgaagat caagcacga gaagcagct ggaaatgct 
      gtcgcatga catccagaa gctgatcga catgtacaa 
      gaatgagga gttggagta tgagatgtc gagcaaaag 
      cgagttttt tgcttaagg accaaatat ttctgtgtt 
      attctagtc ttgatgttt tgtttcaga tactcgcct 
      taaattgga attggtact aaatagtta caaatttct 
      gttcctagc gttctgttg tggttaaaa tgttactaa 
      atataaatt ttatgtcag ttaagttta gggaaaaat 
      ttaaaagta aagtacccc gattataaa tataaatgt 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY