Status
Please wait ...
Gene information
General information
TC008012 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 3879445..3882323
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008012
    iBeetle screen iB_01302
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    maxillary palp shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    maxillary palp orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    vitellarium size decreased - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008012 RA
      MFSKKKKKP QISPPTNFE HRVHTGFDK REGRYVGLP 
      LQWASIVGN NQILKSTNR PLPLVDPSE ITPTEILDL 
      KTIVRGDHR SDSRLGKNP PNGILLPKT SNVARSNSL 
      RSSSPPRLR HRNTNVPPA VPEEQILQY TSMRRDPSM 
      SKPQVRESS PAERSLSNH EMPHHLNGN QEAYGGLIR 
      EHPQVSPAG SMASAGLSQ LGAHSHSSH GSHQSLQQA 
      GYGVRHDQN QNLKNGGQM GQVPGAAVA QTATKHHEQ 
      RLTHEQFRA ALQMVVSSK DPRENLERF VKIGEGSTG 
      TVCIAHDRT TGRQVAVKK MDLRKQQRR ELLFNEVVI 
      MRDYHHPNI VEMFDSYLV NDELWVVME FLEGGALTD 
      IVTHSRMDE EQIATVCKQ CLKALAYLH SQGVIHRDI 
      KSDSILLAL DGRVKLSDF GFCAQVSQE LPKRKSLVG 
      TPYWMSPEV ISRLPYGPE VDIWSLGIM VIEMVDGEP 
      PFFNEPPLQ AMRRIREMP PPKLKNSHK VSARLQSFL 
      DRMLVRDPA QRATAQELL AHPFLRQAG PPALLVPLM 
      RGAKHSNY
    CDS sequence
    • >TC008012 RA
      atgttttcg aaaaagaag aagaaaccg caaatttcg 
      cccccaaca aacttcgag catcgagtc cacaccggg 
      tttgacaaa cgcgagggg cgctatgtg gggctgcca 
      ctgcagtgg gcctcgatt gtgggtaac aaccaaatc 
      ctcaaatca acaaatcgg cccctgccc ctcgtggac 
      ccctccgaa ataaccccc acggaaatc ctagacctc 
      aaaaccatc gtcagaggg gaccaccgc tccgacagc 
      cgccttggc aaaaacccc cccaacggc attctcctc 
      cccaaaacc tccaacgtc gcacgcagc aactcgctg 
      cgctcctcc agccccccg agactgcgc cacaggaac 
      accaacgtg cccccggca gtgcccgag gagcagata 
      ctacagtac acctccatg aggagggac cccagcatg 
      agcaagccc caagtgagg gaaagctcg cctgcggag 
      cgctctttg agcaaccac gagatgccc caccatttg 
      aatgggaat caggaggcc tacgggggg ctgatcagg 
      gagcacccg caagtgtct ccagccgga tccatggcc 
      tcagcgggg ctgtcgcaa ttgggggcg cactcgcat 
      tcgagtcac ggatcgcat cagagtttg caacaggcg 
      gggtacggg gtcaggcac gatcaaaat cagaatttg 
      aagaatggg gggcagatg gggcaggtg ccgggggcg 
      gcggtggca cagactgcg acgaagcat cacgaacaa 
      aggctgaca cacgaacag ttccgagct gctctccag 
      atggtcgtc tcgtcgaag gaccctcgc gagaacctg 
      gagcgtttc gtgaaaatc ggcgaaggc tccaccggc 
      accgtttgc atcgcccac gaccgcacc accgggcgt 
      caagtcgcc gtcaaaaaa atggatctc cgcaaacag 
      caacgtcgc gaactcctc ttcaacgag gtggtgata 
      atgcgcgac taccaccac cccaacatc gtcgaaatg 
      ttcgactcg tacctcgtc aacgacgag ctgtgggtc 
      gtgatggag ttcctcgaa ggtggggct ctcaccgac 
      atcgtcact cattcccgc atggacgaa gaacaaatc 
      gccacggtg tgcaaacag tgtctcaaa gctttggct 
      tatctccat tcgcaaggc gtcatccat cgcgatatc 
      aagtccgat tcgattttg ctggctctg gatggaaga 
      gtcaagctt tccgacttc gggttctgt gcccaggtc 
      tcccaggag ttgcccaaa cggaagagt ttggtcggg 
      acgccgtat tggatgtcg ccggaagtc atcagtcgg 
      ttgccgtac gggccggaa gttgatatt tggtcgctg 
      gggattatg gttatagag atggtggac ggcgagccg 
      ccgtttttc aatgagcct ccattgcag gcgatgaga 
      agaatcaga gaaatgccg ccgccgaaa ttgaaaaat 
      agtcataaa gtctcagca aggctgcaa agtttctta 
      gatagaatg cttgttaga gatcctgca cagagagcc 
      acagctcag gagttgctc gcgcatccc ttcctgaga 
      caagcagga ccgcctgcg cttctggtg ccgctgatg 
      agaggcgct aaacactct aattattga
    mRNA sequence
    • >TC008012 RA
      ctcgaatcg cttaaatcg tcgaagttg agtgttttg 
      gtgtcatgt tttcttaat ttcctcagt ttttcgtct 
      ttaatttca cttaaaatc aacaaataa tcaaaaact 
      agtgccttc cctctagtt actatacta aaaaagtgt 
      gaaaatgtc tcgtgacgt atcaaataa acaatcgag 
      ttcaggtta tgtgtgttg acactgtta ccgttggtc 
      gtaaaaaac ggtgtaaag gggcgtagc aacctactt 
      gtgattccg tctcagacc gaaatgttt tcgaaaaag 
      aagaagaaa ccgcaaatt tcgccccca acaaacttc 
      gagcatcga gtccacacc gggtttgac aaacgcgag 
      gggcgctat gtggggctg ccactgcag tgggcctcg 
      attgtgggt aacaaccaa atcctcaaa tcaacaaat 
      cggcccctg cccctcgtg gacccctcc gaaataacc 
      cccacggaa atcctagac ctcaaaacc atcgtcaga 
      ggggaccac cgctccgac agccgcctt ggcaaaaac 
      ccccccaac ggcattctc ctccccaaa acctccaac 
      gtcgcacgc agcaactcg ctgcgctcc tccagcccc 
      ccgagactg cgccacagg aacaccaac gtgcccccg 
      gcagtgccc gaggagcag atactacag tacacctcc 
      atgaggagg gaccccagc atgagcaag ccccaagtg 
      agggaaagc tcgcctgcg gagcgctct ttgagcaac 
      cacgagatg ccccaccat ttgaatggg aatcaggag 
      gcctacggg gggctgatc agggagcac ccgcaagtg 
      tctccagcc ggatccatg gcctcagcg gggctgtcg 
      caattgggg gcgcactcg cattcgagt cacggatcg 
      catcagagt ttgcaacag gcggggtac ggggtcagg 
      cacgatcaa aatcagaat ttgaagaat ggggggcag 
      atggggcag gtgccgggg gcggcggtg gcacagact 
      gcgacgaag catcacgaa caaaggctg acacacgaa 
      cagttccga gctgctctc cagatggtc gtctcgtcg 
      aaggaccct cgcgagaac ctggagcgt ttcgtgaaa 
      atcggcgaa ggctccacc ggcaccgtt tgcatcgcc 
      cacgaccgc accaccggg cgtcaagtc gccgtcaaa 
      aaaatggat ctccgcaaa cagcaacgt cgcgaactc 
      ctcttcaac gaggtggtg ataatgcgc gactaccac 
      caccccaac atcgtcgaa atgttcgac tcgtacctc 
      gtcaacgac gagctgtgg gtcgtgatg gagttcctc 
      gaaggtggg gctctcacc gacatcgtc actcattcc 
      cgcatggac gaagaacaa atcgccacg gtgtgcaaa 
      cagtgtctc aaagctttg gcttatctc cattcgcaa 
      ggcgtcatc catcgcgat atcaagtcc gattcgatt 
      ttgctggct ctggatgga agagtcaag ctttccgac 
      ttcgggttc tgtgcccag gtctcccag gagttgccc 
      aaacggaag agtttggtc gggacgccg tattggatg 
      tcgccggaa gtcatcagt cggttgccg tacgggccg 
      gaagttgat atttggtcg ctggggatt atggttata 
      gagatggtg gacggcgag ccgccgttt ttcaatgag 
      cctccattg caggcgatg agaagaatc agagaaatg 
      ccgccgccg aaattgaaa aatagtcat aaagtctca 
      gcaaggctg caaagtttc ttagataga atgcttgtt 
      agagatcct gcacagaga gccacagct caggagttg 
      ctcgcgcat cccttcctg agacaagca ggaccgcct 
      gcgcttctg gtgccgctg atgagaggc gctaaacac 
      tctaattat tgatctgta cttaatgca tatatgttg 
      atatttatg gaggtaagt cccaatctt tgcgtaatt 
      taagtcata ccaaatatt aattaagac agctctctt 
      aattcgatc tgattaaac gttattaag gtccagtat 
      tacgaaatt gcaagccgt ccatgtagt gccttgagg 
      ctcataatg gggtttttc caattattt tccgatttt 
      gttaggttt aagcttgtt gagtatttt aaggaatta 
      actttacga cgtagcgct gtaatatat acaaacaga 
      aagtgtcat aatacactt gatattttt ta