General information | |||
TC008012 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3879445..3882323 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008012 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01302 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)maxillary palp shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
maxillary palp orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
vitellarium size decreased - Number of animals showing the phenotype: 4
mature egg not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
- >TC008012 RA
MFSKKKKKP QISPPTNFE HRVHTGFDK REGRYVGLP LQWASIVGN NQILKSTNR PLPLVDPSE ITPTEILDL KTIVRGDHR SDSRLGKNP PNGILLPKT SNVARSNSL RSSSPPRLR HRNTNVPPA VPEEQILQY TSMRRDPSM SKPQVRESS PAERSLSNH EMPHHLNGN QEAYGGLIR EHPQVSPAG SMASAGLSQ LGAHSHSSH GSHQSLQQA GYGVRHDQN QNLKNGGQM GQVPGAAVA QTATKHHEQ RLTHEQFRA ALQMVVSSK DPRENLERF VKIGEGSTG TVCIAHDRT TGRQVAVKK MDLRKQQRR ELLFNEVVI MRDYHHPNI VEMFDSYLV NDELWVVME FLEGGALTD IVTHSRMDE EQIATVCKQ CLKALAYLH SQGVIHRDI KSDSILLAL DGRVKLSDF GFCAQVSQE LPKRKSLVG TPYWMSPEV ISRLPYGPE VDIWSLGIM VIEMVDGEP PFFNEPPLQ AMRRIREMP PPKLKNSHK VSARLQSFL DRMLVRDPA QRATAQELL AHPFLRQAG PPALLVPLM RGAKHSNY
- >TC008012 RA
atgttttcg aaaaagaag aagaaaccg caaatttcg cccccaaca aacttcgag catcgagtc cacaccggg tttgacaaa cgcgagggg cgctatgtg gggctgcca ctgcagtgg gcctcgatt gtgggtaac aaccaaatc ctcaaatca acaaatcgg cccctgccc ctcgtggac ccctccgaa ataaccccc acggaaatc ctagacctc aaaaccatc gtcagaggg gaccaccgc tccgacagc cgccttggc aaaaacccc cccaacggc attctcctc cccaaaacc tccaacgtc gcacgcagc aactcgctg cgctcctcc agccccccg agactgcgc cacaggaac accaacgtg cccccggca gtgcccgag gagcagata ctacagtac acctccatg aggagggac cccagcatg agcaagccc caagtgagg gaaagctcg cctgcggag cgctctttg agcaaccac gagatgccc caccatttg aatgggaat caggaggcc tacgggggg ctgatcagg gagcacccg caagtgtct ccagccgga tccatggcc tcagcgggg ctgtcgcaa ttgggggcg cactcgcat tcgagtcac ggatcgcat cagagtttg caacaggcg gggtacggg gtcaggcac gatcaaaat cagaatttg aagaatggg gggcagatg gggcaggtg ccgggggcg gcggtggca cagactgcg acgaagcat cacgaacaa aggctgaca cacgaacag ttccgagct gctctccag atggtcgtc tcgtcgaag gaccctcgc gagaacctg gagcgtttc gtgaaaatc ggcgaaggc tccaccggc accgtttgc atcgcccac gaccgcacc accgggcgt caagtcgcc gtcaaaaaa atggatctc cgcaaacag caacgtcgc gaactcctc ttcaacgag gtggtgata atgcgcgac taccaccac cccaacatc gtcgaaatg ttcgactcg tacctcgtc aacgacgag ctgtgggtc gtgatggag ttcctcgaa ggtggggct ctcaccgac atcgtcact cattcccgc atggacgaa gaacaaatc gccacggtg tgcaaacag tgtctcaaa gctttggct tatctccat tcgcaaggc gtcatccat cgcgatatc aagtccgat tcgattttg ctggctctg gatggaaga gtcaagctt tccgacttc gggttctgt gcccaggtc tcccaggag ttgcccaaa cggaagagt ttggtcggg acgccgtat tggatgtcg ccggaagtc atcagtcgg ttgccgtac gggccggaa gttgatatt tggtcgctg gggattatg gttatagag atggtggac ggcgagccg ccgtttttc aatgagcct ccattgcag gcgatgaga agaatcaga gaaatgccg ccgccgaaa ttgaaaaat agtcataaa gtctcagca aggctgcaa agtttctta gatagaatg cttgttaga gatcctgca cagagagcc acagctcag gagttgctc gcgcatccc ttcctgaga caagcagga ccgcctgcg cttctggtg ccgctgatg agaggcgct aaacactct aattattga
- >TC008012 RA
ctcgaatcg cttaaatcg tcgaagttg agtgttttg gtgtcatgt tttcttaat ttcctcagt ttttcgtct ttaatttca cttaaaatc aacaaataa tcaaaaact agtgccttc cctctagtt actatacta aaaaagtgt gaaaatgtc tcgtgacgt atcaaataa acaatcgag ttcaggtta tgtgtgttg acactgtta ccgttggtc gtaaaaaac ggtgtaaag gggcgtagc aacctactt gtgattccg tctcagacc gaaatgttt tcgaaaaag aagaagaaa ccgcaaatt tcgccccca acaaacttc gagcatcga gtccacacc gggtttgac aaacgcgag gggcgctat gtggggctg ccactgcag tgggcctcg attgtgggt aacaaccaa atcctcaaa tcaacaaat cggcccctg cccctcgtg gacccctcc gaaataacc cccacggaa atcctagac ctcaaaacc atcgtcaga ggggaccac cgctccgac agccgcctt ggcaaaaac ccccccaac ggcattctc ctccccaaa acctccaac gtcgcacgc agcaactcg ctgcgctcc tccagcccc ccgagactg cgccacagg aacaccaac gtgcccccg gcagtgccc gaggagcag atactacag tacacctcc atgaggagg gaccccagc atgagcaag ccccaagtg agggaaagc tcgcctgcg gagcgctct ttgagcaac cacgagatg ccccaccat ttgaatggg aatcaggag gcctacggg gggctgatc agggagcac ccgcaagtg tctccagcc ggatccatg gcctcagcg gggctgtcg caattgggg gcgcactcg cattcgagt cacggatcg catcagagt ttgcaacag gcggggtac ggggtcagg cacgatcaa aatcagaat ttgaagaat ggggggcag atggggcag gtgccgggg gcggcggtg gcacagact gcgacgaag catcacgaa caaaggctg acacacgaa cagttccga gctgctctc cagatggtc gtctcgtcg aaggaccct cgcgagaac ctggagcgt ttcgtgaaa atcggcgaa ggctccacc ggcaccgtt tgcatcgcc cacgaccgc accaccggg cgtcaagtc gccgtcaaa aaaatggat ctccgcaaa cagcaacgt cgcgaactc ctcttcaac gaggtggtg ataatgcgc gactaccac caccccaac atcgtcgaa atgttcgac tcgtacctc gtcaacgac gagctgtgg gtcgtgatg gagttcctc gaaggtggg gctctcacc gacatcgtc actcattcc cgcatggac gaagaacaa atcgccacg gtgtgcaaa cagtgtctc aaagctttg gcttatctc cattcgcaa ggcgtcatc catcgcgat atcaagtcc gattcgatt ttgctggct ctggatgga agagtcaag ctttccgac ttcgggttc tgtgcccag gtctcccag gagttgccc aaacggaag agtttggtc gggacgccg tattggatg tcgccggaa gtcatcagt cggttgccg tacgggccg gaagttgat atttggtcg ctggggatt atggttata gagatggtg gacggcgag ccgccgttt ttcaatgag cctccattg caggcgatg agaagaatc agagaaatg ccgccgccg aaattgaaa aatagtcat aaagtctca gcaaggctg caaagtttc ttagataga atgcttgtt agagatcct gcacagaga gccacagct caggagttg ctcgcgcat cccttcctg agacaagca ggaccgcct gcgcttctg gtgccgctg atgagaggc gctaaacac tctaattat tgatctgta cttaatgca tatatgttg atatttatg gaggtaagt cccaatctt tgcgtaatt taagtcata ccaaatatt aattaagac agctctctt aattcgatc tgattaaac gttattaag gtccagtat tacgaaatt gcaagccgt ccatgtagt gccttgagg ctcataatg gggtttttc caattattt tccgatttt gttaggttt aagcttgtt gagtatttt aaggaatta actttacga cgtagcgct gtaatatat acaaacaga aagtgtcat aatacactt gatattttt ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
