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Gene information
General information
TC007995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3665645..3667448
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007995
    iBeetle screen iB_01300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007995 RA
      MEPKKKCSH IIRRSKYER NAAEVTNWW HRLFSLLES 
      EAAIPPDRR LDVVNEIFP EKLLNRILS ILSFVRSCK 
      RPTTDDESP KPPDDQKTD DSIPTEDHQ RKEVGPHNP 
      YTRFFRRRP DRAAIWHTL PPLSLEEMS LTQKAEAIT 
      TLIATEFVE WMENISDED SPTTLTVPL VLKMFEIGF 
      DTHAARSLQ VRVKEMASV PDGVAILKA MPDMAKRSQ 
      LHRQLLRDL KASQQKKRI FAFGTKIDK EHQVIPPKM 
      ADIDKWLVC KRVPEHLES MAAVWQGIT HLRSTRAYC 
      EYLLEHPEI TPPKYLVQM RMLDKETLK GQIHLQADE 
      KETGPTIIP KRTMETEAH HAEQEAIML GN
    CDS sequence
    • >TC007995 RA
      atggagccc aaaaagaag tgctcgcac attatccgc 
      cggagtaaa tatgaacgg aacgcagcg gaagttaca 
      aactggtgg catagattg tttagcttg ctggaatcc 
      gaggcggcc atcccgccc gatcgacga ctcgacgtc 
      gtaaacgag atattccca gagaaactt ctcaaccgg 
      atccttagc attttgagc ttcgtcagg agctgcaaa 
      cgcccaacc accgatgac gaatccccc aaacccccc 
      gacgaccaa aaaaccgac gactcaatc cccaccgaa 
      gaccaccaa cgaaaagag gtcggacct cacaacccc 
      tacacgcgc ttcttccgg cggcggccc gaccgagca 
      gctatctgg cacacctta ccgccctta tcactggaa 
      gagatgagt ctgactcaa aaggcagag gcgattacg 
      acactgata gcgaccgag tttgtcgaa tggatggag 
      aacataagc gacgaggat tcgcccacg accttgacc 
      gtgcccctg gtactgaag atgttcgaa atcgggttc 
      gatacgcac gctgcaagg tcgctgcaa gtccgcgtc 
      aaggagatg gcctcggtg ccggacggc gtcgccata 
      ctcaaagcc atgccagac atggcgaag cgaagccaa 
      ctgcaccgg caactactc cgggatttg aaagcgtcg 
      cagcagaag aagaggatt ttcgctttc gggacgaaa 
      atcgacaag gagcatcaa gtgatccct ccaaagatg 
      gcagatata gacaaatgg ttggtttgc aagagagtt 
      ccggagcat ctcgaatca atggcggcg gtgtggcag 
      ggaatcacc cacttgaga tcgaccaga gcctactgc 
      gagtatctt ctcgagcat cccgaaatt acgccgccc 
      aaatatttg gtgcaaatg cgaatgctg gataaagaa 
      acgctcaaa ggacaaatc catcttcag gcggatgag 
      aaagaaacg ggtcctact ataataccg aaaaggaca 
      atggaaaca gaagcgcac cacgcggag caggaagct 
      attatgtta gggaattag
    mRNA sequence
    • >TC007995 RA
      tgtttcaaa agggaattg cataacgtt attttttta 
      ttaagcaaa aatacaaaa tattaaata aaacctcga 
      cgaaatttc gatcgaagc tcaaatcga ccaaacttg 
      ttaccaagc aaaagtgta cctgccagc tgtcataac 
      tcgaaatgg agcccaaaa agaagtgct cgcacatta 
      tccgccgga gtaaatatg aacggaacg cagcggaag 
      ttacaaact ggtggcata gattgttta gcttgctgg 
      aatccgagg cggccatcc cgcccgatc gacgactcg 
      acgtcgtaa acgagatat tcccagaga aacttctca 
      accggatcc ttagcattt tgagcttcg tcaggagct 
      gcaaacgcc caaccaccg atgacgaat cccccaaac 
      cccccgacg accaaaaaa ccgacgact caatcccca 
      ccgaagacc accaacgaa aagaggtcg gacctcaca 
      acccctaca cgcgcttct tccggcggc ggcccgacc 
      gagcagcta tctggcaca ccttaccgc ccttatcac 
      tggaagaga tgagtctga ctcaaaagg cagaggcga 
      ttacgacac tgatagcga ccgagtttg tcgaatgga 
      tggagaaca taagcgacg aggattcgc ccacgacct 
      tgaccgtgc ccctggtac tgaagatgt tcgaaatcg 
      ggttcgata cgcacgctg caaggtcgc tgcaagtcc 
      gcgtcaagg agatggcct cggtgccgg acggcgtcg 
      ccatactca aagccatgc cagacatgg cgaagcgaa 
      gccaactgc accggcaac tactccggg atttgaaag 
      cgtcgcagc agaagaaga ggattttcg ctttcggga 
      cgaaaatcg acaaggagc atcaagtga tccctccaa 
      agatggcag atatagaca aatggttgg tttgcaaga 
      gagttccgg agcatctcg aatcaatgg cggcggtgt 
      ggcagggaa tcacccact tgagatcga ccagagcct 
      actgcgagt atcttctcg agcatcccg aaattacgc 
      cgcccaaat atttggtgc aaatgcgaa tgctggata 
      aagaaacgc tcaaaggac aaatccatc ttcaggcgg 
      atgagaaag aaacgggtc ctactataa taccgaaaa 
      ggacaatgg aaacagaag cgcaccacg cggagcagg 
      aagctatta tgttaggga attagcgta gagaactgt 
      aatagggga ctggctgaa gcgccaccg aaccagtca 
      ataaataaa gttggatgt ttccgttta tttgcgaac 
      ttggcccta atttttagg aaatgcgcc tgtttgaga 
      cggctgttc cggattgaa aattaattc aattgcctt 
      caaaccgaa ttaaccaaa tttctagtg gcacactta 
      aattttttc ttcactgaa tctaaaaat aaatatttt 
      tttatctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY