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Gene information
General information
TC007993 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3548230..3552198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007993
    iBeetle screen iB_01299

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007993 RA
      MLDLFTIFS KGGIVLWCF QSTSQAFTS SVNALIRNV 
      ILQERTGVH NFDHDGLTL QYKLDNEFD LVFVVAYQK 
      ILQLSYVDK FLNDVHLEF RDKYKNDLA DGRYFQEFD 
      FVDRFNATL QAAEQWGKT QAKAPKAMR TFEQSNKSK 
      KTVASMIER KGEEKKQKK KEVNFSEEP PKTPPSPKE 
      NNHVIEEDV IAANRAKFA QKMNKKKSE PKKSPKPAA 
      EKAGKKPRI WDLGGTNKD LETLERTTD KPEDIKSHF 
      TPDTEIVGK MKGVIKDLE VESSSDEEY EEEEAPSQQ 
      TKPASRGGM FSIFKGLVG SKNLTAADM QPALDKMKD 
      HLIAKNVAA DIAIKLCES VALKLEGKV LGTFESVAS 
      TVKSTLTES LVQILSPKR RVDILRDCM EAQRSGRPY 
      VMTFCGVNG VGKSTNLAK ICFWLIENN MRVLIAACD 
      TFRAGAVEQ LRTHMRHLN ALHPPERHG GRQMVQLYE 
      KGYGKDAAG IAMEAIKYA TDSRFDVVL IDTAGRMQD 
      NEPLMRALT KLIKVNEPD LVLFVGEAL VGNEAVDQL 
      VKFNQALAD YSSSSNPHI IDGIVLTKF DTIDDKVGA 
      AISMTYITG QPIVFVGTG QTYTDLKAL NAKAVVHSL 
      MK
    CDS sequence
    • >TC007993 RA
      atgttggac ttatttact atttttagt aaaggaggc 
      attgttttg tggtgcttc caaagcacg agtcaagcg 
      tttacatcc tcggtaaac gctttgatc cgcaatgtg 
      atcctccag gaacgtaca ggggtccac aatttcgac 
      cacgacggt ctgaccctc caatacaaa ctagacaac 
      gagttcgat ttagttttc gttgtcgcg tatcaaaaa 
      atcctgcag ctgtcgtac gtggacaag tttttaaac 
      gacgtccat ttggagttt agggataag tacaaaaat 
      gacctcgcg gatggaaga tatttccag gagtttgat 
      ttcgtggat aggtttaac gcgacgcta caggcggcc 
      gagcaatgg ggcaaaacc caagccaag gcccctaaa 
      gctatgcgt acttttgag cagtcgaat aagtcgaaa 
      aaaaccgta gcttctatg attgagcgg aagggagag 
      gagaaaaaa cagaagaaa aaagaagtc aatttcagc 
      gaggaaccc ccgaaaacg ccgccctcg cccaaagaa 
      aataaccac gtgattgag gaggacgta attgcggct 
      aacagggcc aagttcgca caaaaaatg aacaagaag 
      aaaagcgaa ccaaaaaag agtccgaaa cccgccgcc 
      gaaaaagcg ggtaagaaa cccagaatt tgggatttg 
      gggggcact aacaaagat ctggaaact ctggaacgg 
      acaactgat aaaccggaa gacatcaaa agccacttt 
      acgccagac actgaaatt gtagggaaa atgaagggc 
      gtaattaaa gatctagaa gtggagtcc tcttcggac 
      gaggaatac gaagaagag gaggcccca agtcaacaa 
      accaagccc gcatccaga ggcggcatg ttctcaatt 
      ttcaagggt cttgttggc tctaaaaac ctgaccgct 
      gcggacatg cagccggcc ctggacaaa atgaaagac 
      cacttaatc gcaaagaac gttgctgct gacattgcc 
      atcaaactc tgcgaatcc gtggcttta aaactcgaa 
      ggtaaagtc ttgggtact tttgaaagt gtcgcctcc 
      acggtcaaa tccaccctg acggaatca ctggtccag 
      attttgagt ccgaaacgt cgtgtcgac atccttcgg 
      gattgtatg gaagcccaa cgttcggga cgtccgtac 
      gtcatgact ttctgtggc gtaaacggc gtcggaaag 
      tccacgaat ttggcaaaa atatgcttc tggcttatt 
      gagaataat atgagagtg ctgatcgca gcttgtgat 
      acgttccgt gccggagca gttgaacaa ttgcgcacg 
      catatgcga catttgaac gctctgcat ccaccggaa 
      cggcacgga ggacgtcag atggtccag ttgtatgaa 
      aaagggtac gggaaggat gcggcaggg attgccatg 
      gaggcgatc aagtatgcc acggattcg agatttgat 
      gttgttttg attgatacg gcggggcgt atgcaggat 
      aatgagccc ctgatgagg gctctaact aagttgatt 
      aaggttaat gagcccgat cttgttttg ttcgtgggg 
      gaagcactg gttgggaat gaagctgtg gaccaactt 
      gtcaaattt aaccaagct ctggccgat tactcgtcg 
      agtagtaac ccgcatatc atcgacggg attgtcttg 
      acgaaattc gacactatc gacgataag gttggggct 
      gccatttcc atgacttac ataacgggc cagccgatc 
      gtgttcgtg ggcaccggc cagacatac accgacctt 
      aaagcattg aacgcaaag gccgtcgta cattctctt 
      atgaaataa
    mRNA sequence
    • >TC007993 RA
      tatgttctt atttaatgg agttgataa agcttggca 
      ctaatttgt ttcgctggt gcttttgtt gtcgctttt 
      tacccttag ttcctcgtt tttccttca cttttattg 
      agttaattc ttttgcact gtaaatgtg agtctagtg 
      gcgacacca acggtccga atcgctggg attggtcaa 
      atccttgag cagatccat cacttcttt gaccaaaat 
      attgacctc ggatagcga ctcgaaacg gaataaacc 
      gctgtgaag gccgctttt caacatgta acatcatta 
      ttaacactt ttgcttcag aatattagg ctcgcatga 
      agtgttaat aaaccactt tccgtgcat gtgcaataa 
      ccgaaacat tccataatc aaattaaga ctttccgtt 
      cattatcac tagttgtag ggtttattg tcgggtgat 
      ttgctaagc tatttttac tgtgctttt gctacacat 
      gtaatgtga caaatgacc aataaataa tcaataact 
      gtttaatcc tttatttaa ataaaaaat atcacaaac 
      aaaatttgg ctgcttaaa gcggagtcg ttgataata 
      ctgataagg tgtaaatac acccagcgc accctaaaa 
      gtagggtca caattgaca ccaaaggat aatcgtcga 
      ggtccccca cagggacaa gcaactcga cttcatcaa 
      acaaagcct gataactga catttctcc aactgcaca 
      aaaccccat tacttatat cacaggggg ctttaattt 
      cgctttggg ggctcaacg ccaaaacgc ttggtttat 
      tcacaataa ctttgccat agccccctg aaatttgcg 
      cccgctggt accgcaaat gaaacggga cgtttagag 
      acactcttt ctgcaaaag ggggcacgt aaatgtaag 
      taatgtgcc ccttggata aatgagcag gtgcctcaa 
      catccacat cgccgacta taagcaccg acaagtggc 
      ccaaacgat tcaaatcgg cgatttgat ccggattta 
      agtacattt cgggcccca catcgagtc gaattgcta 
      gtttgcact gaagggagg tggggcccc gctcaagga 
      gccacttga gctcccggt ggaagccct cattatcga 
      tcttttggg ttagagaga cgttcaggc tttggcaag 
      gggagtctt ttgatgaat ttgggctga ttagtagta 
      aaataatga gctgaaatg acaggggtt aacacggaa 
      gggaggtta ggacgtgtt accatttga aacggcctt 
      ggagccaat gcgaactgc tttaaatgc ataataact 
      gggtttttt aaatgtcag tcacttcat tatcatagt 
      tctggaaat ttggggcca gttattcaa ttgtattga 
      aatttcgag ttgacattg acatttggc acgttcgac 
      acttgctca caaatgaat taaaaatat cgagaaatt 
      attgctaaa attgatgcc agaatccgg atttgggcc 
      ttagttagc cggataagt cacttttgc atcgaaatt 
      ttgcctgaa agtatcaaa aatcgtgat taaaagtgt 
      ggcggtaac gtagatttc tgtaatttt gacagtgac 
      agttcccaa ctgacgtgt atcgtgctt gtgagattt 
      ttgaataat gttggactt atttactat ttttagtaa 
      aggaggcat tgttttgtg gtgcttcca aagcacgag 
      tcaagcgtt tacatcctc ggtaaacgc tttgatccg 
      caatgtgat cctccagga acgtacagg ggtccacaa 
      tttcgacca cgacggtct gaccctcca atacaaact 
      agacaacga gttcgattt agttttcgt tgtcgcgta 
      tcaaaaaat cctgcagct gtcgtacgt ggacaagtt 
      tttaaacga cgtccattt ggagtttag ggataagta 
      caaaaatga cctcgcgga tggaagata tttccagga 
      gtttgattt cgtggatag gtttaacgc gacgctaca 
      ggcggccga gcaatgggg caaaaccca agccaaggc 
      ccctaaagc tatgcgtac ttttgagca gtcgaataa 
      gtcgaaaaa aaccgtagc ttctatgat tgagcggaa 
      gggagagga gaaaaaaca gaagaaaaa agaagtcaa 
      tttcagcga ggaaccccc gaaaacgcc gccctcgcc 
      caaagaaaa taaccacgt gattgagga ggacgtaat 
      tgcggctaa cagggccaa gttcgcaca aaaaatgaa 
      caagaagaa aagcgaacc aaaaaagag tccgaaacc 
      cgccgccga aaaagcggg taagaaacc cagaatttg 
      ggatttggg gggcactaa caaagatct ggaaactct 
      ggaacggac aactgataa accggaaga catcaaaag 
      ccactttac gccagacac tgaaattgt agggaaaat 
      gaagggcgt aattaaaga tctagaagt ggagtcctc 
      ttcggacga ggaatacga agaagagga ggccccaag 
      tcaacaaac caagcccgc atccagagg cggcatgtt 
      ctcaatttt caagggtct tgttggctc taaaaacct 
      gaccgctgc ggacatgca gccggccct ggacaaaat 
      gaaagacca cttaatcgc aaagaacgt tgctgctga 
      cattgccat caaactctg cgaatccgt ggctttaaa 
      actcgaagg taaagtctt gggtacttt tgaaagtgt 
      cgcctccac ggtcaaatc caccctgac ggaatcact 
      ggtccagat tttgagtcc gaaacgtcg tgtcgacat 
      ccttcggga ttgtatgga agcccaacg ttcgggacg 
      tccgtacgt catgacttt ctgtggcgt aaacggcgt 
      cggaaagtc cacgaattt ggcaaaaat atgcttctg 
      gcttattga gaataatat gagagtgct gatcgcagc 
      ttgtgatac gttccgtgc cggagcagt tgaacaatt 
      gcgcacgca tatgcgaca tttgaacgc tctgcatcc 
      accggaacg gcacggagg acgtcagat ggtccagtt 
      gtatgaaaa agggtacgg gaaggatgc ggcagggat 
      tgccatgga ggcgatcaa gtatgccac ggattcgag 
      atttgatgt tgttttgat tgatacggc ggggcgtat 
      gcaggataa tgagcccct gatgagggc tctaactaa 
      gttgattaa ggttaatga gcccgatct tgttttgtt 
      cgtggggga agcactggt tgggaatga agctgtgga 
      ccaacttgt caaatttaa ccaagctct ggccgatta 
      ctcgtcgag tagtaaccc gcatatcat cgacgggat 
      tgtcttgac gaaattcga cactatcga cgataaggt 
      tggggctgc catttccat gacttacat aacgggcca 
      gccgatcgt gttcgtggg caccggcca gacatacac 
      cgaccttaa agcattgaa cgcaaaggc cgtcgtaca 
      ttctcttat gaaataagc ctcttccgc aaaacgcaa 
      ttgtaaata atttacctg aaactgtat atttattta 
      ataacggag gtttaacac agattttgt aattctaag 
      gtgaaaagt gaataaatt tttgtctgt tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY