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Gene information
General information
TC007979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3370860..3371995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007979
      iBeetle screen iB_01296

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007979 RA
        MEEEEENAN PVEEDNPPD EPPPPEPEP EPEPVIEET 
        PKEVTINEP DAPKPPVDA PTTNQDALS MEFLYYGSV 
        LKVIAPTLS SDRDKEMAL PWIRKLFRP EYQTSKLRE 
        KRNRYLISL TINLLNDEI SGVFEKSPP DDALADLRS 
        ILPDPSTAA EWEADRMWQ ETLLSLSDD FEMMGCCVH 
        GSKEECQQD HNLDKVLDQ EFQYLLYLT RPYAALLPA 
        EDKTRTAAW LQTLCTIHG EACSAMKAI RNDYIMALV 
        GYLHDLRIV GPFTDYPPD FLEPLNKAA KKSARINPV 
        IDPTGPEAN QFLLDQPVP DDGAFCYVA LTGDLVASN 
        L
      CDS sequence
      • >TC007979 RA
        atggaagaa gaagaagaa aacgctaat cccgtagaa 
        gaagataat cctcccgat gaaccaccg ccgccggaa 
        ccggaaccg gaaccggaa cccgtcatt gaagaaaca 
        ccaaaagaa gttacaata aacgaaccg gacgcacca 
        aaaccccct gtggacgct cccacaacg aaccaagac 
        gccctcagt atggaattc ctttattac ggatctgtc 
        ctcaaagtc atagcaccc actttgagc agcgaccgg 
        gataaagaa atggcgctt ccttggatc cggaaattg 
        ttccggccc gagtatcag acctcaaaa ctgcgagaa 
        aaacgaaac cgatattta atttcattg actattaat 
        ttattgaac gatgagatt agcggtgtc ttcgagaaa 
        agtcccccg gacgacgct ttggccgac ttgaggtct 
        atactgccg gatccttca acggcggca gaatgggaa 
        gccgaccgc atgtggcag gaaacactc ttgtctctg 
        tcggacgac ttcgaaatg atgggttgt tgcgtgcac 
        gggtcgaaa gaagagtgc cagcaggac cacaacttg 
        gataaagtt ttggatcag gaattccag tatttgtta 
        tacttgacg cgtccttac gccgccctg ctccctgcc 
        gaggacaag acgcgcacg gcggcttgg ctccagact 
        ctgtgtacg attcacggc gaggcttgt tccgcaatg 
        aaagccata agaaatgac tacattatg gcccttgtt 
        ggctatttg cacgatttg aggatcgtg ggcccgttc 
        acagactac ccgcccgac tttttggaa cctttgaat 
        aaagccgcg aaaaagtcc gcccggatt aatccggtg 
        attgatccg actggtccc gaagcgaat caattttta 
        ttggatcaa ccggttccg gatgatggc gcgttctgt 
        tatgtggct ctgacggga gatttggta gccagcaat 
        ttgtaa
      mRNA sequence
      • >TC007979 RA
        agtcagggg aaaatttaa cgtttcggt tgtcacgtc 
        aaaattcgc aatggaaga agaagaaga aaacgctaa 
        tcccgtaga agaagataa tcctcccga tgaaccacc 
        gccgccgga accggaacc ggaaccgga acccgtcat 
        tgaagaaac accaaaaga agttacaat aaacgaacc 
        ggacgcacc aaaaccccc tgtggacgc tcccacaac 
        gaaccaaga cgccctcag tatggaatt cctttatta 
        cggatctgt cctcaaagt catagcacc cactttgag 
        cagcgaccg ggataaaga aatggcgct tccttggat 
        ccggaaatt gttccggcc cgagtatca gacctcaaa 
        actgcgaga aaaacgaaa ccgatattt aatttcatt 
        gactattaa tttattgaa cgatgagat tagcggtgt 
        cttcgagaa aagtccccc ggacgacgc tttggccga 
        cttgaggtc tatactgcc ggatccttc aacggcggc 
        agaatggga agccgaccg catgtggca ggaaacact 
        cttgtctct gtcggacga cttcgaaat gatgggttg 
        ttgcgtgca cgggtcgaa agaagagtg ccagcagga 
        ccacaactt ggataaagt tttggatca ggaattcca 
        gtatttgtt atacttgac gcgtcctta cgccgccct 
        gctccctgc cgaggacaa gacgcgcac ggcggcttg 
        gctccagac tctgtgtac gattcacgg cgaggcttg 
        ttccgcaat gaaagccat aagaaatga ctacattat 
        ggcccttgt tggctattt gcacgattt gaggatcgt 
        gggcccgtt cacagacta cccgcccga ctttttgga 
        acctttgaa taaagccgc gaaaaagtc cgcccggat 
        taatccggt gattgatcc gactggtcc cgaagcgaa 
        tcaattttt attggatca accggttcc ggatgatgg 
        cgcgttctg ttatgtggc tctgacggg agatttggt 
        agccagcaa tttgtaacg aataaatga cttgaagga 
        ct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY