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Gene information
General information
TC007964 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3234748..3236171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007964
    iBeetle screen iB_01294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007964 RA
      MPAAVGCIN HSSNEECGI RDVWAHNLE EEFRTIRQI 
      VQKYHYVAM DTEFPGVVA RPIGEFKSS ADYQYQMLR 
      CNVDLLRII QLGLTFLDD NGKTPGGAY TTWQFNFKF 
      NLQEDMYAQ DSIDLLTNS GIQFKKHED EGIEPLDFA 
      ELLMTSGIV LMDNIKWLS FHSGYDFGY LIKLLTDNH 
      LPQDENEFF ELLKLYFPA IYDVKYLMK SCKNLKGGL 
      QEVAEQLDL ERVGPQHQA GSDSLLTGM AFFKMKEMF 
      FEDTIDDSK FSGHLYGLG TSFAVNGTS NNYASDNGE 
      NTNS
    CDS sequence
    • >TC007964 RA
      atgccagcg gctgtgggg tgcattaac cactcaagc 
      aacgaggag tgcggcatt cgggacgtc tgggctcac 
      aatttggaa gaggaattt agaacaata agacaaatt 
      gtacagaag taccattat gtggccatg gacacggaa 
      tttcccggc gtggtggcc cgacccatc ggcgaattt 
      aagagctca gcggattac cagtaccaa atgctgaga 
      tgtaacgtc gatttgctg cggattata caactgggg 
      ctcacgttt ctagacgac aatggcaaa acgcccggg 
      ggcgcatac acaacgtgg cagttcaat tttaaattt 
      aatttacag gaagatatg tacgcccaa gacagcatc 
      gatttgtta acaaattcc gggatccag ttcaaaaaa 
      cacgaagac gagggaatt gaaccttta gattttgcc 
      gaactgctc atgacgtcg ggcatcgtc cttatggac 
      aatatcaaa tggctgtcg tttcattcg ggctacgac 
      ttcggttat ttaataaaa cttctaacc gacaaccat 
      ttgcctcaa gacgaaaac gagtttttt gagttgctt 
      aagctgtat ttccccgcc atttatgac gtaaagtat 
      ttgatgaaa tcgtgtaaa aacttgaaa ggggggctg 
      caagaagtc gcagaacag ttagattta gaacgagtg 
      ggtccacaa catcaagct ggatctgat tcgctgtta 
      actggaatg gcgtttttt aaaatgaag gaaatgttt 
      tttgaggat acgattgat gattcaaaa ttttcggga 
      catctgtac gggctgggg acatcgttc gccgtcaat 
      ggaacttcc aataactat gcctctgat aatggagaa 
      aataccaat tcatga
    mRNA sequence
    • >TC007964 RA
      tgtattgcc cacaacgtc atcgatttt gattatttg 
      gatgaaaac aactcgaca aaagcatgt tggaaaagt 
      cgaattaga tatgaaatt cacaaaact gtagaacac 
      tttgttaat actcgatta agaagggcc cgctgttac 
      cgtactaaa gagcaatgc cagcggctg tggggtgca 
      ttaaccact caagcaacg aggagtgcg gcattcggg 
      acgtctggg ctcacaatt tggaagagg aatttagaa 
      caataagac aaattgtac agaagtacc attatgtgg 
      ccatggaca cggaatttc ccggcgtgg tggcccgac 
      ccatcggcg aatttaaga gctcagcgg attaccagt 
      accaaatgc tgagatgta acgtcgatt tgctgcgga 
      ttatacaac tggggctca cgtttctag acgacaatg 
      gcaaaacgc ccgggggcg catacacaa cgtggcagt 
      tcaatttta aatttaatt tacaggaag atatgtacg 
      cccaagaca gcatcgatt tgttaacaa attccggga 
      tccagttca aaaaacacg aagacgagg gaattgaac 
      ctttagatt ttgccgaac tgctcatga cgtcgggca 
      tcgtcctta tggacaata tcaaatggc tgtcgtttc 
      attcgggct acgacttcg gttatttaa taaaacttc 
      taaccgaca accatttgc ctcaagacg aaaacgagt 
      tttttgagt tgcttaagc tgtatttcc ccgccattt 
      atgacgtaa agtatttga tgaaatcgt gtaaaaact 
      tgaaagggg ggctgcaag aagtcgcag aacagttag 
      atttagaac gagtgggtc cacaacatc aagctggat 
      ctgattcgc tgttaactg gaatggcgt tttttaaaa 
      tgaaggaaa tgttttttg aggatacga ttgatgatt 
      caaaatttt cgggacatc tgtacgggc tggggacat 
      cgttcgccg tcaatggaa cttccaata actatgcct 
      ctgataatg gagaaaata ccaattcat gaaaatctg 
      tcttaattt atgttttaa ttgttcaca gtggtcaca 
      ttttgtgta aataagaac gcctctttt tgtattgtt 
      ttataattt gccacgaga ttacttaat aagaaaaac 
      catcgttta gtgactgta ctggtttta ttctccatt 
      aaataagac ttttctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY