Status
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Gene information
General information
TC032786
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007961,TC007960,TC007962
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3218004..3220680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032786
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01293

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032786 RA
        MKNISLLLC LSIGIQSCI SATVEDAET QLKFLREKT 
        SNKLETAFL NQTRMSNDI DATALAAKQ YGRMEIEKK 
        TDKYNAKLQ ADKALIYRC RGSRKHIYE HTKNSNIED 
        LIRCVDQQG RQSVNVLSE QRQGLQKYV WDAIYEAER 
        QIIVCGQSS KCLEAAVAD IKDKIDEVS KVIDAELDI 
        SQHNKEVQV QVIKACASN SIAHAEQVF STIPTYIFL 
        LVVLTLQLS YCATIQEVK SALTRLRET TEDRLQNAT 
        LKHTKMSNE IDSVALWTK ELGKKQINQ KARKFLAKA 
        RAEKRTNPK CRGLRENAI NHLKKTHTE ELLKCVENH 
        STSTLKLMC EMKSALNEK IWKGVSEEE KNMIVCGQS 
        DTLCLDGVI ANIKDKIES LDEMKNTIL LLFVVYTIQ 
        LCNSATVED AETELKRLR KLATHKLQA ASFEYNKIS 
        EEVDSAAKA EQDYGHFEI HQKSNNLKT KFNQDHGIS 
        HKCRGSRHH LIHQLGESH IADLQRCID HQRAEIANV 
        LSTQKLALD EQVWSAVYE EEKHIVLCG LDRGCLDGA 
        IKNVKEKIA EVMKVINAN ISSSRQLKD AQVLLIKSC 
        ARRSVGNAG HALDNVPHC SY
      CDS sequence
      • >TC032786 RA
        atgaagaac atttcttta ctactttgc ctttcgata 
        ggaattcag tcttgcatt tctgcaacg gtagaagac 
        gcagaaacg caattaaaa ttccttcga gagaaaacc 
        tcaaataaa ttagaaacg gcgttttta aaccagaca 
        agaatgtca aatgatatt gatgcaacc gctcttgcc 
        gctaaacaa tacggaaga atggaaatt gaaaagaaa 
        acagacaaa tacaacgct aagcttcaa gcagacaaa 
        gctttaata tacagatgt cgaggctca cggaaacac 
        atctacgaa cacacgaaa aactcaaat atcgaagat 
        ttaattcgc tgcgttgat caacaggga cgtcagagt 
        gttaacgtt ttgtcagag caaagacag ggtttgcaa 
        aagtacgtc tgggacgcg atttacgaa gctgagagg 
        caaattatt gtttgcggt caatctagc aagtgtctt 
        gaagctgcc gttgccgat attaaagat aagattgac 
        gaagtttcc aaagtgatt gatgctgaa cttgacatc 
        agtcaacat aataaagag gtgcaagtc caggttatt 
        aaagcttgt gctagcaat agtatcgca catgctgag 
        caagttttc agtacaatt cctacatac atattcctg 
        ttggttgtg ttgactctt cagcttagt tattgtgcc 
        acaatacaa gaagttaaa tcagcctta actagactt 
        agagaaacc acggaagat cgcctccaa aacgccact 
        ttaaaacac acaaaaatg tcaaatgaa attgactca 
        gttgccctc tggaccaaa gaactgggt aaaaaacaa 
        ataaaccaa aaagcgaga aaattctta gctaaagca 
        cgagctgag aaaagaacg aatcccaaa tgtagaggt 
        ttacgtgaa aatgccata aaccacttg aaaaaaacc 
        cacactgaa gaactgctg aaatgtgtt gaaaatcac 
        tcaactagc actttgaaa ttaatgtgc gaaatgaag 
        tcggcacta aacgagaaa atttggaaa ggtgtttct 
        gaagaagaa aagaacatg atagtgtgt ggtcagtcg 
        gacactttg tgtctggat ggtgtgatt gcaaatatt 
        aaagataaa attgagtct ttggatgag atgaaaaat 
        acaattttg ctccttttc gtcgtatac acaatccag 
        ttgtgtaac tcagctacg gtcgaagac gccgagact 
        gagctcaaa agactccga aaactggcc actcataaa 
        ctccaagct gcatcattt gaatacaac aaaatatct 
        gaagaagtt gactcagca gcgaaagct gagcaagac 
        tatggccat ttcgaaatt caccaaaaa tccaacaac 
        ttgaagacc aagttcaat caagatcac gggatttcg 
        cacaagtgt cgtggttca agacaccac cttatccac 
        cagctgggg gaatcacac attgctgat ctgcaacgc 
        tgcattgac catcagcgt gcagaaatc gccaatgtt 
        ttgtccaca caaaaacta gcattggac gaacaggtc 
        tggagtgca gtttacgag gaggaaaag cacattgtt 
        ctgtgcggt ctggataga ggttgtctt gatggtgcg 
        attaaaaat gttaaggag aaaattgcc gaagtgatg 
        aaggtgatt aatgcaaat atcagctcc agtcggcag 
        ctgaaagac gcccaagtg cttttgatc aagtcatgt 
        gccaggaga agtgtgggc aatgctgga catgcactg 
        gataatgta ccccactgt tcctattaa
      mRNA sequence
      • >TC032786 RA
        caacatgaa gaacatttc tttactact ttgcctttc 
        gataggaat tcagtcttg catttctgc aacggtaga 
        agacgcaga aacgcaatt aaaattcct tcgagagaa 
        aacctcaaa taaattaga aacggcgtt tttaaacca 
        gacaagaat gtcaaatga tattgatgc aaccgctct 
        tgccgctaa acaatacgg aagaatgga aattgaaaa 
        gaaaacaga caaatacaa cgctaagct tcaagcaga 
        caaagcttt aatatacag atgtcgagg ctcacggaa 
        acacatcta cgaacacac gaaaaactc aaatatcga 
        agatttaat tcgctgcgt tgatcaaca gggacgtca 
        gagtgttaa cgttttgtc agagcaaag acagggttt 
        gcaaaagta cgtctggga cgcgattta cgaagctga 
        gaggcaaat tattgtttg cggtcaatc tagcaagtg 
        tcttgaagc tgccgttgc cgatattaa agataagat 
        tgacgaagt ttccaaagt gattgatgc tgaacttga 
        catcagtca acataataa agaggtgca agtccaggt 
        tattaaagc ttgtgctag caatagtat cgcacatgc 
        tgagcaagt tttcagtac aattcctac atacatatt 
        cctgttggt tgtgttgac tcttcagct tagttattg 
        tgccacaat acaagaagt taaatcagc cttaactag 
        acttagaga aaccacgga agatcgcct ccaaaacgc 
        cactttaaa acacacaaa aatgtcaaa tgaaattga 
        ctcagttgc cctctggac caaagaact gggtaaaaa 
        acaaataaa ccaaaaagc gagaaaatt cttagctaa 
        agcacgagc tgagaaaag aacgaatcc caaatgtag 
        aggtttacg tgaaaatgc cataaacca cttgaaaaa 
        aacccacac tgaagaact gctgaaatg tgttgaaaa 
        tcactcaac tagcacttt gaaattaat gtgcgaaat 
        gaagtcggc actaaacga gaaaatttg gaaaggtgt 
        ttctgaaga agaaaagaa catgatagt gtgtggtca 
        gtcggacac tttgtgtct ggatggtgt gattgcaaa 
        tattaaaga taaaattga gtctttgga tgagatgaa 
        aaatacaat tttgctcct tttcgtcgt atacacaat 
        ccagttgtg taactcagc tacggtcga agacgccga 
        gactgagct caaaagact ccgaaaact ggccactca 
        taaactcca agctgcatc atttgaata caacaaaat 
        atctgaaga agttgactc agcagcgaa agctgagca 
        agactatgg ccatttcga aattcacca aaaatccaa 
        caacttgaa gaccaagtt caatcaaga tcacgggat 
        ttcgcacaa gtgtcgtgg ttcaagaca ccaccttat 
        ccaccagct gggggaatc acacattgc tgatctgca 
        acgctgcat tgaccatca gcgtgcaga aatcgccaa 
        tgttttgtc cacacaaaa actagcatt ggacgaaca 
        ggtctggag tgcagttta cgaggagga aaagcacat 
        tgttctgtg cggtctgga tagaggttg tcttgatgg 
        tgcgattaa aaatgttaa ggagaaaat tgccgaagt 
        gatgaaggt gattaatgc aaatatcag ctccagtcg 
        gcagctgaa agacgccca agtgctttt gatcaagtc 
        atgtgccag gagaagtgt gggcaatgc tggacatgc 
        actggataa tgtacccca ctgttccta ttaaatgat 
        tattgtatt ttgattctt taaataaac gcagttttc 
        gtta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY