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Gene information
General information
TC007958 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3210988..3213287
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007958
      iBeetle screen iB_01291

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007958 RA
        MFSKTLKRF FAAFLLLQV CKADLADDA 
        EAALNVLRQ ATSDNLNGG FLEHIKMSV 
        EVDTVSLAT KGDALNNHQ SPKVVNDIV 
        DCVNKQAEE AVNLIYSKS SGLTDRVLK 
        TIYDEEKEI VFCGRADDA CLTRVISDI 
        KQKTEAVPT VVHEEIEID RKAVEEEVV 
        AIKACADKV
      CDS sequence
      • >TC007958 RA
        atgttcagc aagacactg aaacgtttt tttgctgct 
        ttcctcctc cttcaggtt tgcaaagcc gatcttgcc 
        gacgatgca gaagcagca ctgaacgtt ttacgacag 
        gcaacaagc gacaatcta aacgggggc tttctggaa 
        cacatcaaa atgtccgtg gaagtggac accgtttcg 
        ctagcgacg aaaggagac gccttgaac aatcaccaa 
        agtccaaaa gttgttaac gacattgta gactgcgtc 
        aacaaacaa gcagaagaa gctgtcaat ctcatttac 
        agcaaaagc tctggcttg accgatcga gtcctcaaa 
        acaatttac gatgaagaa aaagaaatc gttttttgt 
        ggtcgcgca gacgacgct tgtctcact cgcgtcatt 
        tcggacatc aagcagaaa acggaagct gtaccgact 
        gtcgtccac gaagaaatt gaaattgac cggaaggcc 
        gtcgaggaa gaagtggtt gcgattaaa gcgtgcgct 
        gacaaagtt taa
      mRNA sequence
      • >TC007958 RA
        taccccaca agtcgtatt caagacaat tgacaagat 
        gttcagcaa gacactgaa acgtttttt tgctgcttt 
        cctcctcct tcaggtttg caaagccga tcttgccga 
        cgatgcaga agcagcact gaacgtttt acgacaggc 
        aacaagcga caatctaaa cgggggctt tctggaaca 
        catcaaaat gtccgtgga agtggacac cgtttcgct 
        agcgacgaa aggagacgc cttgaacaa tcaccaaag 
        tccaaaagt tgttaacga cattgtaga ctgcgtcaa 
        caaacaagc agaagaagc tgtcaatct catttacag 
        caaaagctc tggcttgac cgatcgagt cctcaaaac 
        aatttacga tgaagaaaa agaaatcgt tttttgtgg 
        tcgcgcaga cgacgcttg tctcactcg cgtcatttc 
        ggacatcaa gcagaaaac ggaagctgt accgactgt 
        cgtccacga agaaattga aattgaccg gaaggccgt 
        cgaggaaga agtggttgc gattaaagc gtgcgctga 
        caaagttta attgccttt cacaaggtt ttgtagtca 
        tttattaga ttttgttac aaataaaca caaggtttt 
        taattaatt ttgatttgc ttcaaatat tacgtaaca 
        tagtaaatg cagtgaata accggttca taattatgc 
        aaataaaaa gttgcatgt cctgtttag ctaaaccat 
        gattgggtt tattaaaat gaggaacaa aatattttt 
        gttcagtgt ttttcttaa ccattgctg aagcaacaa 
        cattttgtt tgtcagaac tgtaggtac gtaattacg 
        ttctcagtt aatttctct ctctccctt tcattttaa 
        tttattaga agcatcatg gttgcacaa agaaataaa 
        atcctcggc aaagagtca acagcgtta ctacttagt 
        ataatgggg ttcctgata tacacattg acgttgtgt 
        gccgtttac tttcaagag aaacccata aaccttgga 
        aactttcgt tcaattttc attgattat cttgccttt 
        ttctactac tcggtgtat ttaattatg atggccttg 
        atttttaaa gaaaaatat gacatcatg tcgggaact 
        ttttcatta atagtgtct ctgttggcc tttttcggt 
        ttggaaatt cttggtttt aattccgtt aattatctt 
        cttcaacat ttggttcac atggctgtg gccagtcta 
        gtgctagga aatgtactt gctttttgg tgttacatt 
        cgtggcttg agcttaccc cccaatgaa ataaaccca 
        aagacaata ggaaaagac acactgtat tgctatatt 
        attggcaga gaagtcagg ccacgtctt ttcggttac 
        ctcgatttg aagatgtat gttaacaga atttgtgtt 
        gtctctgcg gtaagttta ctttctgtt ttaaagctt 
        ttaatagtt gatttaagg ttttgctgg attgtgcct 
        tcttgtatc aagaattga tacgaagtt tgacattac 
        aatcatacg atttgaaca aaagtttta ttgttatgg 
        aagtaattc atttaattt attgcttcg acggcgtgg 
        ttgccgaat acacatatg aatattccc tggaaatcc 
        aaaaagacg gtttcggat acgtgtcag cagttggat 
        atttcatat acccgttta taataagca cagttcctt 
        atcactttc ttcaagcca aaaccgagc tagcagctt 
        ggaagttag taacaataa tatgtttat ttaccgttg 
        gttttctac tgtttagaa tcgcaaata atcagaaat 
        acaacttca aagtaaatg cccagaaac aaggtaaat 
        catatatta atttaatta acgatatat ctaagtaag 
        ttacattta aggggcgaa atttccgac aaactcaaa 
        aaataaaaa attactgtg ttcagggct gtggggatt 
        tgttcgcca tcctaatta cctgggaga cattttaat 
        gaacatttc gtatactgg cttgactat aagtacaac 
        tgtgatatt acctttgtt ggaagtacc tttcttgat 
        tcatcgttc aatcagaga caataaact ttgcaggga 
        aaagtatgg tgacttgtg gcacgaata ttgcgaaaa 
        agttaaata tgttttaat ccctcggat ctattgaca 
        caatatcat ttctgtttt tttatatat tttaacgtt 
        gtttagtta tttttatta gcgaatgta attaagtat 
        ggcaataaa tcaatcttc attat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY