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Gene information
General information
TC007936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2931494..2933607
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007936
    iBeetle screen iB_01287

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 25.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 25.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007936 RA
      MPPTKNPDL QSVSAFLMY RKTSTNKEI TKKRKNLKK 
      NKKSQNIKK KIEEEITKT LPLKPSRRH SHSIKKTRD 
      NQPEQIKKP LPSKKVNVR HSPERDFLP SRKLNGNHN 
      QDSCDESIT QSGFLPVDD PTEDAQKLF EWLIQPLSP 
      ASFFKTYWE QKPLYIKRG NRSYYTHIL DSSSLDKIL 
      RNNSLFFTR NVDVVTYEN GEKQVFNQE GRATPSALW 
      DYYGNGCSI RVLNPQTYN HKVHLLLAT LQEYFGTMV 
      GANVYLTPP GSQGFAPHY DDIEAFVVQ LEGRKHWKL 
      YQPKSEDVL ARFSSPNFK REDLGEPFM ELTLNAGEL 
      LYFPRGTIH EGRTDEDSH SLHITVSVY QQTSYVDLL 
      EHILPKALK KAADSDVEF RKGLPLNYL KDFGLAAGS 
      KNRKFVTSK IKDLMNSLI NYVDIDSAA DQLGKKFMH 
      DSMPPVLTK SEAERSSKA DGPVLKDGV VKNRVEIDL 
      DTRVRLLRY YCIRLVCEE NSSPKLYYN TQNATVYHG 
      EEEQWLELE PSMVPLIQM LQNTYPRYI EVEKLPLDD 
      MATKMQLVL DLWEHGLLV TEYPLDVIE Y
    CDS sequence
    • >TC007936 RA
      atgcctccc acaaaaaat cctgatttg caatcggtg 
      tctgcgttc ttgatgtac aggaagaca tcgaccaat 
      aaagaaatc accaagaaa cgcaaaaat ttaaagaaa 
      aataagaaa tcacaaaat attaagaag aaaatcgaa 
      gaagaaatt acaaaaacc cttcctcta aaaccaagt 
      agaagacac tctcacagt attaaaaaa actcgtgac 
      aatcaacca gagcaaatt aaaaaacct cttccttca 
      aaaaaagtt aatgtgagg cactctcct gaacgagat 
      tttcttcct tcaagaaaa ttaaatggc aatcacaat 
      caagactct tgtgatgaa agcataact caaagtggg 
      tttttgcca gttgatgat ccaacggaa gatgcacaa 
      aagttgttc gagtggctg atacagccc ctttcccct 
      gcctctttc ttcaaaacg tattgggaa cagaaaccc 
      ctttacata aaacggggg aacaggtcg tactacacc 
      cacatttta gattcaagc agtttggac aaaatcctt 
      cgaaacaat agtttattt tttacacga aacgttgac 
      gtcgtgacg tatgaaaac ggcgaaaag caagtattc 
      aaccaggag ggccgagcg actccgtcc gccctttgg 
      gactactac ggtaacggc tgcagtatt cgcgtatta 
      aaccctcaa acttacaac cacaaagtc cacttactt 
      ctggcgact cttcaagaa tattttggc acaatggtc 
      ggggccaac gtatattta acaccgccg ggaagtcaa 
      ggcttcgcc ccgcattac gacgacatt gaggccttt 
      gttgtacag ttggaaggc cgaaagcac tggaagctg 
      taccaacca aaaagtgaa gatgtttta gccagattt 
      tcaagtcct aatttcaaa cgtgaggac ctaggggag 
      cctttcatg gaactgact ttaaatgcc ggcgaactg 
      ctgtatttc ccaaggggc acaatacat gagggtcgc 
      actgatgaa gactcacat tcgttgcac atcacagtc 
      tccgtttac caacaaacg tcttatgtg gatttgttg 
      gaacacatt ttgcccaaa gcgttgaaa aaagccgcc 
      gatagtgac gttgaattt cgtaaagga ctcccgctt 
      aattattta aaagatttc ggattggcc gctgggtca 
      aaaaatagg aaattcgtc acttcaaaa attaaagat 
      ttgatgaat tctttaatc aactacgtg gatattgat 
      agtgcagct gatcagttg ggcaagaaa tttatgcat 
      gactccatg cccccagtt ttgacaaaa agcgaagcg 
      gaaagatcc tctaaagct gatgggcca gttttaaaa 
      gatggtgtt gttaaaaac agagtggaa attgactta 
      gacacgaga gtacgtttg ttgcggtat tattgcatc 
      cgtttagtt tgtgaagaa aactcgtcg ccaaaactc 
      tattacaac actcaaaac gctacagtt taccacggt 
      gaagaggaa cagtggttg gaattggag ccttcgatg 
      gtgccctta attcagatg cttcaaaac acgtatcct 
      cgctacatt gaagtggaa aaattacca ctggatgat 
      atggccacc aaaatgcag ctagtttta gatttatgg 
      gaacacggg ctcttagtc accgagtat cccttagat 
      gtgatagag tattaa
    mRNA sequence
    • >TC007936 RA
      gcagagtaa aaatgagaa aaattaacg accaaaaaa 
      ttatttaaa atttaaaat ttgttaata cgattaaag 
      gtccaagaa aaatgtttt tgtaacagt gagtgccaa 
      cattttacg tgtggggaa tttctcgtg agtgagctt 
      tctcgtcta agaattttc gcgcgatca ggtttcacg 
      tggtgaatt tttagtgtt gttattttg caataaata 
      aaatgcctc ccacaaaaa atcctgatt tgcaatcgg 
      tgtctgcgt tcttgatgt acaggaaga catcgacca 
      ataaagaaa tcaccaaga aacgcaaaa atttaaaga 
      aaaataaga aatcacaaa atattaaga agaaaatcg 
      aagaagaaa ttacaaaaa cccttcctc taaaaccaa 
      gtagaagac actctcaca gtattaaaa aaactcgtg 
      acaatcaac cagagcaaa ttaaaaaac ctcttcctt 
      caaaaaaag ttaatgtga ggcactctc ctgaacgag 
      attttcttc cttcaagaa aattaaatg gcaatcaca 
      atcaagact cttgtgatg aaagcataa ctcaaagtg 
      ggtttttgc cagttgatg atccaacgg aagatgcac 
      aaaagttgt tcgagtggc tgatacagc ccctttccc 
      ctgcctctt tcttcaaaa cgtattggg aacagaaac 
      ccctttaca taaaacggg ggaacaggt cgtactaca 
      cccacattt tagattcaa gcagtttgg acaaaatcc 
      ttcgaaaca atagtttat tttttacac gaaacgttg 
      acgtcgtga cgtatgaaa acggcgaaa agcaagtat 
      tcaaccagg agggccgag cgactccgt ccgcccttt 
      gggactact acggtaacg gctgcagta ttcgcgtat 
      taaaccctc aaacttaca accacaaag tccacttac 
      ttctggcga ctcttcaag aatattttg gcacaatgg 
      tcggggcca acgtatatt taacaccgc cgggaagtc 
      aaggcttcg ccccgcatt acgacgaca ttgaggcct 
      ttgttgtac agttggaag gccgaaagc actggaagc 
      tgtaccaac caaaaagtg aagatgttt tagccagat 
      tttcaagtc ctaatttca aacgtgagg acctagggg 
      agcctttca tggaactga ctttaaatg ccggcgaac 
      tgctgtatt tcccaaggg gcacaatac atgagggtc 
      gcactgatg aagactcac attcgttgc acatcacag 
      tctccgttt accaacaaa cgtcttatg tggatttgt 
      tggaacaca ttttgccca aagcgttga aaaaagccg 
      ccgatagtg acgttgaat ttcgtaaag gactcccgc 
      ttaattatt taaaagatt tcggattgg ccgctgggt 
      caaaaaata ggaaattcg tcacttcaa aaattaaag 
      atttgatga attctttaa tcaactacg tggatattg 
      atagtgcag ctgatcagt tgggcaaga aatttatgc 
      atgactcca tgcccccag ttttgacaa aaagcgaag 
      cggaaagat cctctaaag ctgatgggc cagttttaa 
      aagatggtg ttgttaaaa acagagtgg aaattgact 
      tagacacga gagtacgtt tgttgcggt attattgca 
      tccgtttag tttgtgaag aaaactcgt cgccaaaac 
      tctattaca acactcaaa acgctacag tttaccacg 
      gtgaagagg aacagtggt tggaattgg agccttcga 
      tggtgccct taattcaga tgcttcaaa acacgtatc 
      ctcgctaca ttgaagtgg aaaaattac cactggatg 
      atatggcca ccaaaatgc agctagttt tagatttat 
      gggaacacg ggctcttag tcaccgagt atcccttag 
      atgtgatag agtattaag ttttgcaga gttacataa 
      taaattttt accaaggct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY