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Gene information
General information
TC007927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2850614..2852676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007927
    iBeetle screen iB_01284
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature mostly centralthinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    Pupal morphology
    eyes surface irregular - Number of animals showing the phenotype: 8

    Comment: the covering cuticle seems to be more prominent
    elytra partially blistered - Number of animals showing the phenotype: 8

    legs shortened - Number of animals showing the phenotype: 8

    legs orientation irregular - Number of animals showing the phenotype: 8
    elytra proximal blistered - Number of animals showing the phenotype: 5

    pronotum shape irregular - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007927 RA
      MSDNLEKLY EKYNILSDA KDKVSQHSS EYVESFEGI 
      KGTESEKML AAQILSKFF KHFPSLQDK ALNALLDLC 
      EDEESKIRA CAMRYLVSI CKDVKEHLT KVTDILAQM 
      MQLEEQRDF TTASWCLLQ LWKQDSANV LRTMYNHIR 
      SLSSAAARV KCLQFIHLK FIKPIESQP TEIENIVVE 
      ESKKLLQDD ISSEEIVLI ISCLKNSKY AKTAAGQQE 
      LLDFISEIM ELDRDFDPL EDCIVDRVI ICTTHALPF 
      FSAKNESTK FVAYYCDQI LPQWDKIAT LEQGELFQL 
      HLLRHLAEL SIYCGKFEN PSLHVVQIF DKIKLYMPT 
      PPENADVYK MPNLEFSFV ECLLFAFHR LARQCPDFL 
      THDPQILKD FRARLVYFS RGVQGCNKV LNSKPITGL 
      DSVNASKAK IAPSLLNNI NVLIKDLFY QPPMYKCNV 
      TLSFKQEVV EKELRKPAT SGQKRHVPI TFDNGSTGT 
      KQTRPARSG DNVKLYTPP SGKFSNNFQ YDRGGSRGN 
      RSRGRGSRG RGRNWRN
    CDS sequence
    • >TC007927 RA
      atgagtgac aacttggag aagttatac gaaaagtat 
      aacattttg tcagatgcc aaagacaag gtctcccag 
      cattcaagc gaatatgtc gagagcttt gaaggtatt 
      aaaggcacc gaaagcgag aaaatgctg gcagcacaa 
      atactttca aaatttttc aaacacttc ccctcactg 
      caagataaa gcgttgaat gcgttgctg gacttgtgt 
      gaagatgag gagagcaag attagagct tgtgccatg 
      cggtatttg gtttcaatt tgcaaggac gtgaaggaa 
      catttgact aaagtcact gatatcctt gcccaaatg 
      atgcagttg gaggaacag cgagacttc acaactgcg 
      tcttggtgt cttttgcag ctgtggaag caagactct 
      gctaatgtt cttaggacc atgtacaat catattcgc 
      tctttaagc agcgctgca gctagagtt aaatgtctc 
      caatttatt cacttgaag tttatcaag cccattgaa 
      agccagccg acagaaatc gagaacatt gtagtagaa 
      gaatcgaaa aagctttta caagatgat attagctct 
      gaggaaatc gtgttgata ataagttgc ttaaaaaat 
      tcaaaatac gctaaaacg gccgcaggt caacaagaa 
      ctgttagat ttcatttcc gaaatcatg gaacttgac 
      cgggatttt gacccgctt gaagactgc attgttgac 
      agggtcatt atttgcacc acacatgct ttaccattt 
      ttttcagca aaaaacgag tcaacaaaa ttcgtggct 
      tattactgc gaccagatt ctcccccag tgggacaaa 
      atcgccact ctggagcaa ggcgaatta ttccagctt 
      catctcctg cggcacttg gccgagctt agcatctat 
      tgcggaaag ttcgaaaac ccctccttg cacgtagtc 
      caaattttc gacaaaatc aagctgtac atgccaacg 
      cctcccgaa aacgccgat gtgtacaaa atgcccaac 
      ttggagttc tcctttgta gagtgtttg ttgttcgcg 
      ttccatcgc ttggctcga cagtgcccc gattttctc 
      actcatgat ccccaaata ttgaaagat ttcagagca 
      agactggtt tatttttct agaggagtg caaggttgc 
      aacaaagtt ctgaactcg aagccgatc acaggccta 
      gattccgtg aacgcttca aaagccaaa attgcgccc 
      tcgctactc aacaacatc aacgttttg atcaaagac 
      ttgttctac cagccccca atgtacaaa tgtaacgtc 
      acgctttct ttcaagcag gaggttgtg gaaaaggaa 
      ttgaggaaa ccggccaca agtgggcaa aaaagacat 
      gtgcctatc acgttcgat aatggctct acagggact 
      aaacagacc cgaccggct cgttcgggc gacaacgtt 
      aaattgtat acaccacct agtggaaag tttagtaat 
      aacttccaa tacgatcgt gggggttca cgaggtaac 
      aggtcgaga ggccgtggc tcccgagga aggggcaga 
      aattggaga aattaa
    mRNA sequence
    • >TC007927 RA
      cggcgattc aaaatggac gatgggcac gctaaaaca 
      acgatgacc ggtttgtga tcaagtttg tgtaaatta 
      tcgaataga attacataa aaacgccac gaaattatt 
      tcatgtttt ataccacat ttcgactaa tagatttat 
      cgaaaaaac agtaagatg agtgacaac ttggagaag 
      ttatacgaa aagtataac attttgtca gatgccaaa 
      gacaaggtc tcccagcat tcaagcgaa tatgtcgag 
      agctttgaa ggtattaaa ggcaccgaa agcgagaaa 
      atgctggca gcacaaata ctttcaaaa tttttcaaa 
      cacttcccc tcactgcaa gataaagcg ttgaatgcg 
      ttgctggac ttgtgtgaa gatgaggag agcaagatt 
      agagcttgt gccatgcgg tatttggtt tcaatttgc 
      aaggacgtg aaggaacat ttgactaaa gtcactgat 
      atccttgcc caaatgatg cagttggag gaacagcga 
      gacttcaca actgcgtct tggtgtctt ttgcagctg 
      tggaagcaa gactctgct aatgttctt aggaccatg 
      tacaatcat attcgctct ttaagcagc gctgcagct 
      agagttaaa tgtctccaa tttattcac ttgaagttt 
      atcaagccc attgaaagc cagccgaca gaaatcgag 
      aacattgta gtagaagaa tcgaaaaag cttttacaa 
      gatgatatt agctctgag gaaatcgtg ttgataata 
      agttgctta aaaaattca aaatacgct aaaacggcc 
      gcaggtcaa caagaactg ttagatttc atttccgaa 
      atcatggaa cttgaccgg gattttgac ccgcttgaa 
      gactgcatt gttgacagg gtcattatt tgcaccaca 
      catgcttta ccatttttt tcagcaaaa aacgagtca 
      acaaaattc gtggcttat tactgcgac cagattctc 
      ccccagtgg gacaaaatc gccactctg gagcaaggc 
      gaattattc cagcttcat ctcctgcgg cacttggcc 
      gagcttagc atctattgc ggaaagttc gaaaacccc 
      tccttgcac gtagtccaa attttcgac aaaatcaag 
      ctgtacatg ccaacgcct cccgaaaac gccgatgtg 
      tacaaaatg cccaacttg gagttctcc tttgtagag 
      tgtttgttg ttcgcgttc catcgcttg gctcgacag 
      tgccccgat tttctcact catgatccc caaatattg 
      aaagatttc agagcaaga ctggtttat ttttctaga 
      ggagtgcaa ggttgcaac aaagttctg aactcgaag 
      ccgatcaca ggcctagat tccgtgaac gcttcaaaa 
      gccaaaatt gcgccctcg ctactcaac aacatcaac 
      gttttgatc aaagacttg ttctaccag cccccaatg 
      tacaaatgt aacgtcacg ctttctttc aagcaggag 
      gttgtggaa aaggaattg aggaaaccg gccacaagt 
      gggcaaaaa agacatgtg cctatcacg ttcgataat 
      ggctctaca gggactaaa cagacccga ccggctcgt 
      tcgggcgac aacgttaaa ttgtataca ccacctagt 
      ggaaagttt agtaataac ttccaatac gatcgtggg 
      ggttcacga ggtaacagg tcgagaggc cgtggctcc 
      cgaggaagg ggcagaaat tggagaaat taatttcaa 
      tcacccttc taacagtat tgcagaaat gcaattcaa 
      atcattatg ttcaatgct tattagtaa gttatcaaa 
      acaatacac aacccgctt gtgt