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Gene information
General information
TC007911 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2620104..2621448
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007911
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01283

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages partially need term not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007911 RA
      MAESVFQTR SLGTAAEGI KDQYADGKA AKVWEIFIG 
      DKNSRTQNY KNFLVGLLK KRGCKRILD VACGTGIDS 
      IMLLEEGFE VVSVDASDK MLKYALKAR WNRRDEPAF 
      YNWVIEEAN WLTLYDDIE QIVGNGFDA VICLGNSFA 
      HLLDSFGDQ REQKQAIKN FEKCVKPGG LLLIDHRNY 
      DHIINTGET PSKCIYYNS KHMTEIKPS VLYVCGKPA 
      LVTLDYLID TSTVYGKSD EINMDHVSQ FRLSYYPHR 
      LEVFKDMLH EAFGEKSLY NIYGDFKSL EEIENPGFY 
      IHVVEKARR MF
    CDS sequence
    • >TC007911 RA
      atggctgaa agtgttttt caaactcgt tctctcgga 
      accgccgcc gaaggtata aaagatcaa tacgccgat 
      ggcaaagct gccaaagtt tgggaaata tttatcggc 
      gacaaaaac tcaagaaca caaaattac aaaaatttc 
      ttggtcggt cttttaaag aaaagggga tgcaaacgc 
      attttagac gtagcctgc gggacgggt attgactcc 
      atcatgctc cttgaggaa ggtttcgaa gtcgtctca 
      gttgacgct tcggacaaa atgctcaaa tatgcctta 
      aaggcccgc tggaatcgc agggacgaa cctgccttc 
      tacaactgg gtgattgaa gaggcaaac tggttgact 
      ctttacgac gatattgag caaattgtg ggcaatgga 
      ttcgacgct gtcatctgt ctgggaaat tcctttgcc 
      catttgttg gactcattc ggagatcag agagaacaa 
      aaacaagcc atcaaaaat ttcgaaaag tgcgtcaaa 
      cccggagga cttcttctg atcgatcac aggaattac 
      gaccacatt ataaacacg ggcgaaacg ccctcgaaa 
      tgtatttat tacaatagt aaacacatg acggaaatc 
      aaaccgtcg gttttgtac gtttgtgga aagccagct 
      ctagtcacg ttggattat ttgatcgac acttcgaca 
      gtgtatgga aagagtgac gaaattaat atggatcat 
      gtcagccaa ttccgattg tcatattat ccacaccga 
      ttagaagtt ttcaaagac atgttacat gaagctttc 
      ggcgaaaaa tcactttac aatatctat ggcgatttc 
      aagagtttg gaagaaatt gaaaaccct ggattttac 
      atccacgtg gtggaaaaa gccaggaga atgttctaa
    mRNA sequence
    • >TC007911 RA
      cactggttc aaatgaata taaaagacg tcttcttcc 
      agagaacaa aacatcacc gtttttgta ttgtattag 
      tgtttgtga cccgctagt cccgaaaat ggctgaaag 
      tgtttttca aactcgttc tctcggaac cgccgccga 
      aggtataaa agatcaata cgccgatgg caaagctgc 
      caaagtttg ggaaatatt tatcggcga caaaaactc 
      aagaacaca aaattacaa aaatttctt ggtcggtct 
      tttaaagaa aaggggatg caaacgcat tttagacgt 
      agcctgcgg gacgggtat tgactccat catgctcct 
      tgaggaagg tttcgaagt cgtctcagt tgacgcttc 
      ggacaaaat gctcaaata tgccttaaa ggcccgctg 
      gaatcgcag ggacgaacc tgccttcta caactgggt 
      gattgaaga ggcaaactg gttgactct ttacgacga 
      tattgagca aattgtggg caatggatt cgacgctgt 
      catctgtct gggaaattc ctttgccca tttgttgga 
      ctcattcgg agatcagag agaacaaaa acaagccat 
      caaaaattt cgaaaagtg cgtcaaacc cggaggact 
      tcttctgat cgatcacag gaattacga ccacattat 
      aaacacggg cgaaacgcc ctcgaaatg tatttatta 
      caatagtaa acacatgac ggaaatcaa accgtcggt 
      tttgtacgt ttgtggaaa gccagctct agtcacgtt 
      ggattattt gatcgacac ttcgacagt gtatggaaa 
      gagtgacga aattaatat ggatcatgt cagccaatt 
      ccgattgtc atattatcc acaccgatt agaagtttt 
      caaagacat gttacatga agctttcgg cgaaaaatc 
      actttacaa tatctatgg cgatttcaa gagtttgga 
      agaaattga aaaccctgg attttacat ccacgtggt 
      ggaaaaagc caggagaat gttctaaat gttttattt 
      gtactattt attaaaata aaaaaatat taataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY