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Gene information
General information
TC007896 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2353382..2378212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007896
    iBeetle screen iB_01281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 10

    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 8


    prothoracic leg tarsus shortened - Number of animals showing the phenotype: 8


    mesothoracic leg tarsus shortened - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007896 RA
      MALFWRTVL GLASITSSM AIFIGGEQD VGSPLRVAQ 
      CRATCLERF SNDGPNGES MCLQGPDCF MCWENCELL 
      QSNFQVWGV MCEEKEICF PGCQEACRF HAEASTQIQ 
      QTQPVIHTK GEGVIQVSE ALARWPRPS SVDQKTPLV 
      YVVMIKSQG GVWRQIIQT LDLTTRVPL NTDKKGAML 
      RVLVVDPQG LVTIYSPDE ASFRENRKR KKTINTEIK 
      ATTSKSLTT DSDAPWTLR EVSLIHQKV LVIGEIAWE 
      PKASRGVYL VTWEVDGGG LKGNLFTDS TCVTLSLWP 
      DTVYHIQVE LVSRIPGVE NLKSEMMDL DTGRAQKVS 
      TESQQDLPE DIDYKSPLL SVLVSAYQG ESTKAKWYS 
      NDAMWGAAL AVVLFAVLL GVVLWRCRR RYGNAIDNM 
      ITGASAIRS QKLVEDFTG YTGFQPIIS VMSTDETKY 
      GKCETPEVT FQGAHTSNF LSESEKTAR HEVQV
    CDS sequence
    • >TC007896 RA
      atggcgttg ttttggcgt acggtcctt ggtctggct 
      tcgatcacg tcgtcgatg gcgattttt attggcgga 
      gagcaggat gtaggaagt cccctaagg gtcgcacaa 
      tgcagagct acttgtctt gaaaggttt tcgaacgac 
      ggtcctaat ggagagtcc atgtgtttg caaggtccc 
      gattgtttc atgtgttgg gaaaactgc gagctgttg 
      cagtccaac ttccaagtg tggggggtc atgtgcgaa 
      gagaaagag atctgtttc cctggctgt caagaagct 
      tgccgattc catgcggaa gcctccacg caaatccag 
      caaacccag ccggtcatc cacaccaaa ggcgaggga 
      gtcatccaa gtgtccgag gcgctggcc cgctggccg 
      cggccgtcg tcagtcgac cagaaaacc cctctagtc 
      tacgtagtg atgatcaag agccaaggc ggcgtctgg 
      cgacaaatc atccagact ctggatcta accacgcgc 
      gttcccctc aacaccgac aagaagggg gcgatgctg 
      cgggtgctc gtggtggac ccccaaggc ctggtcaca 
      atttacagc cccgacgag gccagtttc cgcgagaat 
      cgcaagcgg aagaagacg ataaacacg gaaattaag 
      gcaacgacg agcaagagt ctgacgact gattcggac 
      gctccctgg acgttgcgc gaggtctcc ctgatccat 
      cagaaagtt ttagtaatc ggggagatc gcctgggag 
      ccgaaagcc agccgcggt gtttacttg gtgacttgg 
      gaagtggac gggggcggg ctgaaaggc aacttgttc 
      accgattcg acttgcgtc actctgtct ctctggccc 
      gatacggtc tatcacatc caagtcgaa ctcgtttcg 
      aggatcccc ggagtcgaa aacctcaaa tcggaaatg 
      atggacttg gacacggga cgagcgcag aaggtctcc 
      actgagagt cagcaagat ctgcccgaa gatatcgat 
      tataaatcg ccccttttg tccgtttta gtgagtgcg 
      tatcagggg gagagcacc aaagcgaag tggtacagc 
      aacgatgcg atgtggggg gcggcgctc gctgtggtg 
      ctcttcgcg gtactcttg ggggtggtg ttgtggcgg 
      tgtaggagg cggtacggc aacgccatc gataacatg 
      atcacgggg gcttccgcc atcaggagc cagaagctg 
      gtcgaggac ttcaccggg tacacaggc ttccaacca 
      ataatatct gtgatgtcg actgacgag accaaatac 
      ggcaaatgt gagacgccg gaagtaaca ttccaaggt 
      gcccacact agcaatttt ttaagtgaa tccgaaaag 
      actgcacga cacgaagtt caagtgtga
    mRNA sequence
    • >TC007896 RA
      ccgcagttc ggattcaac actcagttg agttcagtt 
      cagttttcg cgtcggaga tggtcgcat gaaagctcg 
      gatgaaagc tgccctcaa agctccacc gcgcgaagt 
      tacccctga aaaagccca aagtgtccg tgctgtggt 
      tgagtttcc tgcatgatc gtccagtgc tttctttat 
      ttttctgtg gatgtgtct agtgcttga attttgatt 
      tgtgctttt ttgattttt ttttgaaaa atggcgttg 
      ttttggcgt acggtcctt ggtctggct tcgatcacg 
      tcgtcgatg gcgattttt attggcgga gagcaggat 
      gtaggaagt cccctaagg gtcgcacaa tgcagagct 
      acttgtctt gaaaggttt tcgaacgac ggtcctaat 
      ggagagtcc atgtgtttg caaggtccc gattgtttc 
      atgtgttgg gaaaactgc gagctgttg cagtccaac 
      ttccaagtg tggggggtc atgtgcgaa gagaaagag 
      atctgtttc cctggctgt caagaagct tgccgattc 
      catgcggaa gcctccacg caaatccag caaacccag 
      ccggtcatc cacaccaaa ggcgaggga gtcatccaa 
      gtgtccgag gcgctggcc cgctggccg cggccgtcg 
      tcagtcgac cagaaaacc cctctagtc tacgtagtg 
      atgatcaag agccaaggc ggcgtctgg cgacaaatc 
      atccagact ctggatcta accacgcgc gttcccctc 
      aacaccgac aagaagggg gcgatgctg cgggtgctc 
      gtggtggac ccccaaggc ctggtcaca atttacagc 
      cccgacgag gccagtttc cgcgagaat cgcaagcgg 
      aagaagacg ataaacacg gaaattaag gcaacgacg 
      agcaagagt ctgacgact gattcggac gctccctgg 
      acgttgcgc gaggtctcc ctgatccat cagaaagtt 
      ttagtaatc ggggagatc gcctgggag ccgaaagcc 
      agccgcggt gtttacttg gtgacttgg gaagtggac 
      gggggcggg ctgaaaggc aacttgttc accgattcg 
      acttgcgtc actctgtct ctctggccc gatacggtc 
      tatcacatc caagtcgaa ctcgtttcg aggatcccc 
      ggagtcgaa aacctcaaa tcggaaatg atggacttg 
      gacacggga cgagcgcag aaggtctcc actgagagt 
      cagcaagat ctgcccgaa gatatcgat tataaatcg 
      ccccttttg tccgtttta gtgagtgcg tatcagggg 
      gagagcacc aaagcgaag tggtacagc aacgatgcg 
      atgtggggg gcggcgctc gctgtggtg ctcttcgcg 
      gtactcttg ggggtggtg ttgtggcgg tgtaggagg 
      cggtacggc aacgccatc gataacatg atcacgggg 
      gcttccgcc atcaggagc cagaagctg gtcgaggac 
      ttcaccggg tacacaggc ttccaacca ataatatct 
      gtgatgtcg actgacgag accaaatac ggcaaatgt 
      gagacgccg gaagtaaca ttccaaggt gcccacact 
      agcaatttt ttaagtgaa tccgaaaag actgcacga 
      cacgaagtt caagtgtga cgacatagt tgtgatgca 
      atcaaaacg actcgcgcc aaactggat gattaaaaa 
      agttcctaa gttttatgt gaaaaaatt gagtgaaaa 
      gtgtcgcga gtgagacaa tttttaaaa tgatcctaa 
      gtgtatatg tgtataaaa actagtcta aattcgaac 
      attttcaac cgctgataa tgtcgaagt tgcttgaga 
      ctgtctaaa ctgattttg ttgcgttgt gtacggaga 
      cgaagcaat tcttcaatc gatgtagat tcgaaatga 
      ctgtgttat tattttgtc aatttttat ttatttttg 
      acgcttaga cttttgtac ataaattta tcccagttt 
      taggacgtc caaaaaccg tgaaaactg taaattgct 
      gataatctt attggaaac tcaataatg tatttgtca 
      atatttgga cagtagctg agtagttat tgttggtta 
      gttagcttc atacatcag atgtgctct ttgtctcgt 
      attttacga ccgggcatc gtcgtgctc gagattttc 
      cttcgaata aatccggcc ctgcttgat tgacctatt 
      ccacaccta ggaaattat aatttttat gaatatttt 
      aagctcgag ccatcgacg tccgcatct aaccgatca 
      ttaaattaa tttcgttgt aaatacttt attgtatta 
      taattatct attatatat catataagt tatttttct 
      attaaacat ggtcttagt cagttccat ggctaaatg 
      taaatataa ttattataa ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY