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Gene information
General information
TC007891 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2279472..2282346
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007891
    iBeetle screen iB_01280
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007891 RA
      MSKINDVTT LYKQLKSEW NNPKPNLAK VEQLLSELK 
      LELTHLMFL PTSNASASK QELLLARDV LEIGVQWSI 
      ASSNIPAFE RYMAQLKCY YFDYKDQLP ESSFKYQLL 
      GLNLLFLLS QNRVAEFHT ELELLPAEH IQNDVYIRH 
      PLSIEQYLM EGSYNKIFL AKGNVPAKN YNFFMDILL 
      DTIRGEIAV CVEKAYEKI SLKDAARML YLANEEAAK 
      TFGKNKKWK LGRDNFFHF APEVKKITE PIPSCELAE 
      QAIEYAKEL EMIV
    CDS sequence
    • >TC007891 RA
      atgtcgaaa attaacgac gttacgacc ctttacaag 
      caactcaag tcagagtgg aacaacccg aagccaaat 
      ctggcaaaa gtcgagcag ttattgtca gaattgaag 
      cttgaatta actcatttg atgtttttg ccgacttcg 
      aatgcgtcc gcttcaaag caagaattg ttactagca 
      cgggacgtt ctagaaatc ggggtacaa tggagcata 
      gcttcgagt aatatcccc gcttttgag cgatatatg 
      gcacaatta aagtgctac tactttgat tacaaagac 
      caactccct gaatcttcc tttaagtat caattgttg 
      ggcttaaat ttgctattt ttgttgtcg caaaaccgc 
      gtcgcggaa tttcacact gagctggaa ctgttgcct 
      gcggagcac attcagaat gatgtttac ataaggcat 
      cctctgtca attgaacag tatttaatg gagggcagt 
      tacaacaag atattttta gccaaaggt aacgtgccg 
      gcaaaaaat tacaacttc ttcatggat attttgctt 
      gacacaatt cgcggcgaa atcgccgtt tgtgtggaa 
      aaagcgtac gaaaaaatc tcgctcaag gatgccgca 
      agaatgtta tatttggca aacgaagaa gccgctaaa 
      actttcggc aaaaataag aagtggaag ttgggccgc 
      gacaacttc ttccacttc gcgcctgag gtcaagaaa 
      atcactgag cccatccca tcgtgcgaa ttggccgag 
      caagcgatt gaatacgcc aaggaatta gaaatgatt 
      gtgtaa
    mRNA sequence
    • >TC007891 RA
      tcgtaaaca tgttttcct ggtggtgtt taatttgga 
      gcgaatcca aggaaaatt ctaacttta ggtttccac 
      caacagcct gacatctac tgacgaaac gcgggacaa 
      gaattgaca gaacaattg tcaaaaatt tgtataaat 
      catccaact atttgttgc aaaaatgtc gaaaattaa 
      cgacgttac gacccttta caagcaact caagtcaga 
      gtggaacaa cccgaagcc aaatctggc aaaagtcga 
      gcagttatt gtcagaatt gaagcttga attaactca 
      tttgatgtt tttgccgac ttcgaatgc gtccgcttc 
      aaagcaaga attgttact agcacggga cgttctaga 
      aatcggggt acaatggag catagcttc gagtaatat 
      ccccgcttt tgagcgata tatggcaca attaaagtg 
      ctactactt tgattacaa agaccaact ccctgaatc 
      ttcctttaa gtatcaatt gttgggctt aaatttgct 
      atttttgtt gtcgcaaaa ccgcgtcgc ggaatttca 
      cactgagct ggaactgtt gcctgcgga gcacattca 
      gaatgatgt ttacataag gcatcctct gtcaattga 
      acagtattt aatggaggg cagttacaa caagatatt 
      tttagccaa aggtaacgt gccggcaaa aaattacaa 
      cttcttcat ggatatttt gcttgacac aattcgcgg 
      cgaaatcgc cgtttgtgt ggaaaaagc gtacgaaaa 
      aatctcgct caaggatgc cgcaagaat gttatattt 
      ggcaaacga agaagccgc taaaacttt cggcaaaaa 
      taagaagtg gaagttggg ccgcgacaa cttcttcca 
      cttcgcgcc tgaggtcaa gaaaatcac tgagcccat 
      cccatcgtg cgaattggc cgagcaagc gattgaata 
      cgccaagga attagaaat gattgtgta atttttgta 
      atttttttt tattgaata aaatagatt ttaagcgat 
      tttattttt ttgtgtaat caaagtcgc cccattcct 
      ttgaaaatt ccactacgc gtttgcttt gctcgtcaa 
      aactttggt atttaggtt tccgccatt tactgtggt 
      gcagatcga gcattccgc cgatgactt cttgcgcaa 
      aattattgg ggaaagttt tctgatcct cgataacgt 
      gagcaaacc cttcttcca ttccaaacg aaacaaaaa 
      aatacggga ggccttttg gaacagccc tgcgcacgt 
      ctttgcctt tcaaagaca ccaattttt tattttggg 
      cccattcgg cctcgcatt cgtctatcg cttttttga 
      aatagatgg gggccaagt cgccctctt ccttaccca 
      aggggatac actcaatgt acatgtggg gatacctat 
      ccagatttt tggccgttt cgaaaaaaa taacgtcca 
      aatttttcg cactaaaca tccttgtta aagcctttc 
      ggaagtcca ttatgtcgc tccattcgt tttcgtcaa 
      gtagcgtgg aacacgtga cgtgtctta tgggggcta 
      tcaagcagt ggccttcgt tcaccggct cgtacggag 
      gcaaactca aaaaggccg tctcgccta acgaaacca 
      tcaaatgtt tggccatgg agtcggact gaagacaca 
      atttacaat tatctatgc tttgagcca ctctttgat 
      gctcgttaa tcgcccggt cacgattcc tcctatcag 
      ttttagccc catcctctt ttttccgaa tgttatcgg 
      aaaatattt catccaaat cgtcgtttt tccggactt 
      tgctcgcca ttcgcataa actcgctat tctggtctt 
      ccactgaat taaatttct cgttttcaa acatttgtt 
      ggagggaat gtttatcat catcggcga aataacgca 
      ctctttgtc catcctgat gagtctcaa ctttgcgtt 
      tccttttac ggctacctg acggttcat aatcggctt 
      gttgtctca cgggtctcg tgtgaccct gagagtcag 
      tttgagtca agagaactt cttccgtag gtttagacg 
      agtctttcc gttgccttg cagcttcaa gtttcgctt 
      tcagttcct tcaccagtt ccgtcttgc ccattattt 
      cggctttta ccagtttag ccgcgaggg cgtttaatt 
      ctttgtccg ataaaattt cgccctgat cttgcgctt 
      ctgggctag tttcacgtt ctttgggct gttctgatg 
      gtttctttt tccagtttg aagatgact gtggcctat 
      tggttgatt tatacgaga aattgtgtt cgtcggacg 
      gttttttaa atttcggta aaccatcac cgccgtcct 
      tccagtacg gatttagtt ctcttgagt gtgatctcg 
      gattgtact gctcacgtt ccctctccc accgtttgt 
      tttcttccc gatttcgcc tcctgtcca aatgcgatt 
      cggttagaa aactggacg gcaaagaca tccattctt 
      ctcgatcag ttgacgatt tttctttag gtttatttt 
      tactggttt cgtcctcag aagacgaat atttgtctt 
      tttcaaccc attcgtctg aagatgagt cagagcccg 
      actcgcgcg cccactccc gttttttgt cttttttat 
      gtttttttg atttctctt tatgtttac cctgtaaa