Status
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Gene information
General information
TC032761
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007884,TC007886,TC007885
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2167972..2205687
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032761
    iBeetle screen iB_01279
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032761 RA
      MASKEKYCS KSDRGAVKI SVIIPRVVM TQVECNRRA 
      VSGLRRKTK APSAGGMRS HSTQLRLFT SSTRHRVFD 
      ARRGHFDPA KASCLRDKF HWGSVLLLQ VNSTGDALE 
      AARNGPDSL NELALVNGA RERTRRSEL SSGKKSPEP 
      FLPRFAEIS AALSWAQQV TVSSPIDVL TPSMDYVSS 
      LCCLLAFVL YYNTLDAGF VYDDRRAIL SNPDLLPKT 
      PWARLLEND FWGTPLSDS GSHGSYRPL CVLTFRLNY 
      LLGGFQPWG YHLVNVLLH CLATALLVK LARQVLPKS 
      KSSVGPAIT GLIFATHPI HTEAVAGVV GRADLAACN 
      FYLLSFLAY VTHTKHRDS FCCYGGNKE DNMKQLRTL 
      KYQKFVYSL QKNVTSCNW PKRLGVSEA CNKNVVFRM 
      HLKEKNEAC CLKSRVRQW MYLGLCILL ALAAMLSKE 
      TGITVLGMC MIYDFIYCP SLKKRQWRS LLVLGAASV 
      ILLAIRLQS RVPQFSTAD NPTAREPKL LTRLLTFIY 
      LPVFNFWLL LVPNTLSFD WGMDAIPRI TTLGDSRNI 
      VTFCFYSLL FLLVKKSVS SMRSPPKTC RCCHLDLSD 
      VHTVNCRNT NNNNTAHST CVCLSKRST SNSCCVILL 
      ALAFLTLPF LPATNLLFY VGFVVAERV LYLPSAGLC 
      LMVGLGGAA LYRKYRTPF TVGFIGVLL TFSAKTVLR 
      NKDWTNEEA LYRAAVNVN PPKAFGNLG SVLSSQGRI 
      TEAEWAFRK ALQFRPNMA DVHYNLGIL LQARQQLGE 
      AIESYQRAI HFRPSLALA YVNLGAALI SAGRCQEAV 
      SVLRQGSRL DGTGLRDRR EHETARVSA LLQLGALYS 
      EQGRLQRAL AAYREAAYS LPEHYPPQS VFNVLGETL 
      ARLQQDEEA ERWYQAALN AQPDHVPAH ITYGKLLAK 
      NVSRTAEAE QWFRKAQRL APQEPSVYH HYGEFLASR 
      RRYKEASVM YEKAAELRP HDYELAVAA ATAMRQAGR 
      HQEAERWYR TAVDLKPSD ARSHTNLGA ILHLNGKYS 
      EAASSYKEA LRLQPDDIT TLTNLHKLH SVMT
    • >TC032761 RB
      MASKEKYCS KSDRGAVKI SVIIPRVVM TQVECNRRA 
      VSGLRRKTK APSAGGMRS HSTQLRLFT SSTRHRVFD 
      ARRGHFDPA KASCLRDKF HWGSVLLLQ VNSTGDALE 
      AARNGPDSL NELALVNGA RERTRRSEL SSGKKSPEP 
      FLPRFAEIS AALSWAQQV TVSSPIDVL TPSMDYVSS 
      LCCLLAFVL YYNTLDAGF VYDDRRAIL SNPDLLPKT 
      PWARLLEND FWGTPLSDS GSHGSYRPL CVLTFRLNY 
      LLGGFQPWG YHLVNVLLH CLATALLVK LARQVLPKS 
      KSSVGPAIT GLIFATHPI HTEAVAGVV GRADLAACN 
      FYLLSFLAY VTHTKHRDS FCCYGGNKE DNMKQLRTL 
      KYQKFVYSL QKNVTSCNW PKRLGVSEA CNKNVVFRM 
      HLKEKNEAC CLKSRVRQW MYLGLCILL ALAAMLSKE 
      TGITVLGMC MIYDFIYCP SLKKRQWRS LLVLGAASV 
      ILLAIRLQS RVPQFSTAD NPTAREPKL LTRLLTFIY 
      LPVFNFWLL LVPNTLSFD WGMDAIPRI TTLGDSRNI 
      VTFCFYSLL FLLVKKSVS SMRSPPKTC RCCHLDLSD 
      VHTVNCRNT NNNNTAHST CVCLSKRST SNSCCVILL 
      ALAFLTLPF LPATNLLFY VGFVVAERV LYLPSAGLC 
      LMVGLGGAA LYRKYRTPF TVGFIGVLL TFSAKTVLR 
      NKDWTNEEA LYRAAVNVN PPKAFGNLG SVLSSQGRI 
      TEAEWAFRK ALQFRPNMA DVHYNLGIL LQARQQLGE 
      AIESYQRAI HFRPSLALA YVNLGAALI SAGRCQEAV 
      SVLRQGSRL DGTGLRDRR EHETARVSA LLQLGALYS 
      EQGRLQRAL AAYREAAYS LPEHYPPQS VFNVLGETL 
      ARLQQDEEA ERWYQAALN AQPDHVPAH ITYGKLLAK 
      NVSRTAEAE QWFRKAQRL APQEPSVYH HYGEFLASR 
      RRYKEASVM YEKAAELRP HDYELAVAA ATAMRQAGR 
      HQEAERWYR TAVDLKPSV SRLNNFIIG KLSMHLCEL 
      LFGFDRARR SKRLD
    CDS sequence
    • >TC032761 RA
      atggcatcg aaagaaaag tactgctca aaatccgac 
      cggggggca gtaaaaatc tctgtaatc attccccgg 
      gtggttatg actcaagta gagtgcaat aggagggct 
      gtgagtgga ctgcgtcgt aaaacaaag gctccttct 
      gccggaggc atgcgttcg cactctaca cagttacgg 
      ctcttcaca tcgtcaaca cggcaccgg gtttttgac 
      gcccgaaga ggccacttt gaccctgcg aaagcttcg 
      tgtttgcga gataaattt cactggggt tccgtcttg 
      ctcctccag gtgaactcc acaggtgac gctctggag 
      gcggcacgc aatggaccc gactcgctt aatgaactc 
      gctctcgtg aacggcgcc cgcgaacgc acccgaaga 
      tcggagctt tcctccggc aaaaagtcg cccgaaccc 
      tttttgccg cgctttgcc gagattagc gcagctttg 
      agttgggcg caacaagtc acggtatct tcgcccatc 
      gatgtgctg acccccagc atggattat gttagctct 
      ctctgctgt cttctcgcc ttcgtcctc tactacaac 
      acgctggat gccggattc gtttacgac gaccggcgg 
      gcgattctg agcaacccc gacctgctg ccgaagacg 
      ccatgggcg cgcctcttg gaaaacgac ttctggggg 
      actcctctc agcgacagc ggctctcac ggctcgtat 
      cgtccgctg tgcgttcta accttccgc ctcaattac 
      cttctggga ggtttccaa ccgtggggc taccacctc 
      gtcaacgtg cttttacac tgtcttgct acggccctt 
      ttggtcaaa ctggcaaga caagtgctt ccgaaatca 
      aagtcttca gtcgggcct gccataacc gggctgatt 
      tttgccacg catcccata cacacggaa gcggtggct 
      ggggttgtg ggccgagct gatttggca gcatgcaat 
      ttttatcta ttatcgttc ctagcctac gttacgcat 
      actaagcat agagattcg ttttgttgt tatggtggt 
      aataaagaa gacaatatg aagcagctt agaactttg 
      aagtaccag aagtttgtt tatagtctg caaaaaaac 
      gttacgagt tgtaattgg ccaaagagg ttgggagtg 
      agcgaggct tgtaataaa aacgtcgtc ttccgaatg 
      catttgaag gagaagaat gaagcgtgt tgtttgaaa 
      agtagagtg aggcaatgg atgtacttg ggtctgtgc 
      attctcctg gctttggct gcgatgctg agcaaggag 
      acggggatc acagtgctg gggatgtgt atgatttac 
      gacttcatt tactgccct tcgttgaaa aagagacaa 
      tggcgcagt ttgctcgtc ctgggagca gcttccgtt 
      atcctttta gccatccgc ttgcaatcc cgcgtgccc 
      cagttctcc accgctgac aatccgacg gctcgcgag 
      ccgaaactc ctcacccgc ttgctcacc ttcatctac 
      cttcccgtc ttcaacttc tggctgctc ctcgtcccc 
      aacaccctg agttttgac tggggcatg gacgccatc 
      ccccgaatc accactctg ggcgacagc cgcaacatc 
      gtcaccttc tgcttctac tcgctgctc ttcctcctg 
      gtgaaaaag agcgtctcg tcgatgaga tcgccgccg 
      aagacgtgc cgctgctgc cacttggac ttgtccgat 
      gtacatacg gttaattgt cggaacacg aacaataat 
      aatacggcg cattcgacg tgcgtctgt ctttcgaaa 
      agatcgaca tcgaattcc tgttgtgtt attttgttg 
      gcgctggcg tttctgact cttcctttt ttgccggcg 
      accaactta ctgttttat gtcgggttt gtggtggcc 
      gagagggtt ctttatcta cccagtgcg ggactgtgt 
      ttgatggtg ggactcggc ggggctgcg ctttatcgg 
      aagtatcgc acgccgttt actgtgggc tttattgga 
      gttttgcta acgttcagt gctaaaacc gtgctgaga 
      aacaaagac tggaccaac gaggaagcg ctgtatcgg 
      gccgccgtc aacgtcaac ccgcccaaa gcgttcggg 
      aatttaggc agcgttttg agcagtcag ggccgaatc 
      acggaagcc gagtgggca ttccgcaag gcgctacaa 
      ttccgaccc aacatggcc gatgtgcac tataatctg 
      ggcattctg ctccaggcg aggcagcaa ttgggcgaa 
      gccatcgag agttaccaa cgagccatt cattttcga 
      ccatcgctg gcactggcc tatgtcaac ttgggcgcc 
      gcgctgata tctgcaggc aggtgtcaa gaagcagtg 
      tcagttctt agacaagga tctcgttta gacgggacg 
      ggtctgagg gaccgacgc gagcacgaa acggccaga 
      gtttcggcc ctcctgcag ctgggagct ttgtattcc 
      gagcaggga cgactgcaa agggcttta gcggcctac 
      agagaagcg gcctactcg ctcccagag cactatcca 
      ccacagagc gttttcaac gtgttggga gagacactc 
      gcccgactc cagcaggac gaggaagcc gaaaggtgg 
      taccaagct gcgctgaat gcacagcct gaccatgtg 
      cctgcccac ataacttac ggcaaactc cttgccaaa 
      aatgtgagc cgaacggcg gaagccgaa cagtggttt 
      cgcaaagcc caaaggttg gccccccag gagcccagc 
      gtctaccac cattacggt gagttcctg gcgtcgcgg 
      cgccgctat aaagaagcg tcggttatg tacgaaaag 
      gcggcagaa ttgcggcca catgactac gagttggcg 
      gtggcggcg gcgacggcg atgaggcag gcgggaagg 
      caccaggag gctgaacgc tggtacaga acggcggta 
      gacctcaag ccttccgat gctcgcagt catacgaat 
      ttgggcgcg atcctccac ctgaacggc aaatatagc 
      gaagctgcc agcagctac aaggaggca ttaaggctt 
      cagccagac gacatcacc acgctgacg aatctgcat 
      aagctgcac agcgtaatg acctga
    • >TC032761 RB
      atggcatcg aaagaaaag tactgctca aaatccgac 
      cggggggca gtaaaaatc tctgtaatc attccccgg 
      gtggttatg actcaagta gagtgcaat aggagggct 
      gtgagtgga ctgcgtcgt aaaacaaag gctccttct 
      gccggaggc atgcgttcg cactctaca cagttacgg 
      ctcttcaca tcgtcaaca cggcaccgg gtttttgac 
      gcccgaaga ggccacttt gaccctgcg aaagcttcg 
      tgtttgcga gataaattt cactggggt tccgtcttg 
      ctcctccag gtgaactcc acaggtgac gctctggag 
      gcggcacgc aatggaccc gactcgctt aatgaactc 
      gctctcgtg aacggcgcc cgcgaacgc acccgaaga 
      tcggagctt tcctccggc aaaaagtcg cccgaaccc 
      tttttgccg cgctttgcc gagattagc gcagctttg 
      agttgggcg caacaagtc acggtatct tcgcccatc 
      gatgtgctg acccccagc atggattat gttagctct 
      ctctgctgt cttctcgcc ttcgtcctc tactacaac 
      acgctggat gccggattc gtttacgac gaccggcgg 
      gcgattctg agcaacccc gacctgctg ccgaagacg 
      ccatgggcg cgcctcttg gaaaacgac ttctggggg 
      actcctctc agcgacagc ggctctcac ggctcgtat 
      cgtccgctg tgcgttcta accttccgc ctcaattac 
      cttctggga ggtttccaa ccgtggggc taccacctc 
      gtcaacgtg cttttacac tgtcttgct acggccctt 
      ttggtcaaa ctggcaaga caagtgctt ccgaaatca 
      aagtcttca gtcgggcct gccataacc gggctgatt 
      tttgccacg catcccata cacacggaa gcggtggct 
      ggggttgtg ggccgagct gatttggca gcatgcaat 
      ttttatcta ttatcgttc ctagcctac gttacgcat 
      actaagcat agagattcg ttttgttgt tatggtggt 
      aataaagaa gacaatatg aagcagctt agaactttg 
      aagtaccag aagtttgtt tatagtctg caaaaaaac 
      gttacgagt tgtaattgg ccaaagagg ttgggagtg 
      agcgaggct tgtaataaa aacgtcgtc ttccgaatg 
      catttgaag gagaagaat gaagcgtgt tgtttgaaa 
      agtagagtg aggcaatgg atgtacttg ggtctgtgc 
      attctcctg gctttggct gcgatgctg agcaaggag 
      acggggatc acagtgctg gggatgtgt atgatttac 
      gacttcatt tactgccct tcgttgaaa aagagacaa 
      tggcgcagt ttgctcgtc ctgggagca gcttccgtt 
      atcctttta gccatccgc ttgcaatcc cgcgtgccc 
      cagttctcc accgctgac aatccgacg gctcgcgag 
      ccgaaactc ctcacccgc ttgctcacc ttcatctac 
      cttcccgtc ttcaacttc tggctgctc ctcgtcccc 
      aacaccctg agttttgac tggggcatg gacgccatc 
      ccccgaatc accactctg ggcgacagc cgcaacatc 
      gtcaccttc tgcttctac tcgctgctc ttcctcctg 
      gtgaaaaag agcgtctcg tcgatgaga tcgccgccg 
      aagacgtgc cgctgctgc cacttggac ttgtccgat 
      gtacatacg gttaattgt cggaacacg aacaataat 
      aatacggcg cattcgacg tgcgtctgt ctttcgaaa 
      agatcgaca tcgaattcc tgttgtgtt attttgttg 
      gcgctggcg tttctgact cttcctttt ttgccggcg 
      accaactta ctgttttat gtcgggttt gtggtggcc 
      gagagggtt ctttatcta cccagtgcg ggactgtgt 
      ttgatggtg ggactcggc ggggctgcg ctttatcgg 
      aagtatcgc acgccgttt actgtgggc tttattgga 
      gttttgcta acgttcagt gctaaaacc gtgctgaga 
      aacaaagac tggaccaac gaggaagcg ctgtatcgg 
      gccgccgtc aacgtcaac ccgcccaaa gcgttcggg 
      aatttaggc agcgttttg agcagtcag ggccgaatc 
      acggaagcc gagtgggca ttccgcaag gcgctacaa 
      ttccgaccc aacatggcc gatgtgcac tataatctg 
      ggcattctg ctccaggcg aggcagcaa ttgggcgaa 
      gccatcgag agttaccaa cgagccatt cattttcga 
      ccatcgctg gcactggcc tatgtcaac ttgggcgcc 
      gcgctgata tctgcaggc aggtgtcaa gaagcagtg 
      tcagttctt agacaagga tctcgttta gacgggacg 
      ggtctgagg gaccgacgc gagcacgaa acggccaga 
      gtttcggcc ctcctgcag ctgggagct ttgtattcc 
      gagcaggga cgactgcaa agggcttta gcggcctac 
      agagaagcg gcctactcg ctcccagag cactatcca 
      ccacagagc gttttcaac gtgttggga gagacactc 
      gcccgactc cagcaggac gaggaagcc gaaaggtgg 
      taccaagct gcgctgaat gcacagcct gaccatgtg 
      cctgcccac ataacttac ggcaaactc cttgccaaa 
      aatgtgagc cgaacggcg gaagccgaa cagtggttt 
      cgcaaagcc caaaggttg gccccccag gagcccagc 
      gtctaccac cattacggt gagttcctg gcgtcgcgg 
      cgccgctat aaagaagcg tcggttatg tacgaaaag 
      gcggcagaa ttgcggcca catgactac gagttggcg 
      gtggcggcg gcgacggcg atgaggcag gcgggaagg 
      caccaggag gctgaacgc tggtacaga acggcggta 
      gacctcaag ccttccgtg agtagatta aacaacttt 
      attataggg aagctttcg atgcacctc tgtgaactt 
      ttattcgga tttgataga gcgcgccga tcgaagcgg 
      cttgactaa
    mRNA sequence
    • >TC032761 RA
      gcatcgaca taaaaccac gtgtaaaat ggcatcgaa 
      agaaaagta ctgctcaaa atccgaccg gggggcagt 
      aaaaatctc tgtaatcat tccccgggt ggttatgac 
      tcaagtaga gtgcaatag gagggctgt gagtggact 
      gcgtcgtaa aacaaaggc tccttctgc cggaggcat 
      gcgttcgca ctctacaca gttacggct cttcacatc 
      gtcaacacg gcaccgggt ttttgacgc ccgaagagg 
      ccactttga ccctgcgaa agcttcgtg tttgcgaga 
      taaatttca ctggggttc cgtcttgct cctccaggt 
      gaactccac aggtgacgc tctggaggc ggcacgcaa 
      tggacccga ctcgcttaa tgaactcgc tctcgtgaa 
      cggcgcccg cgaacgcac ccgaagatc ggagctttc 
      ctccggcaa aaagtcgcc cgaaccctt tttgccgcg 
      ctttgccga gattagcgc agctttgag ttgggcgca 
      acaagtcac ggtatcttc gcccatcga tgtgctgac 
      ccccagcat ggattatgt tagctctct ctgctgtct 
      tctcgcctt cgtcctcta ctacaacac gctggatgc 
      cggattcgt ttacgacga ccggcgggc gattctgag 
      caaccccga cctgctgcc gaagacgcc atgggcgcg 
      cctcttgga aaacgactt ctgggggac tcctctcag 
      cgacagcgg ctctcacgg ctcgtatcg tccgctgtg 
      cgttctaac cttccgcct caattacct tctgggagg 
      tttccaacc gtggggcta ccacctcgt caacgtgct 
      tttacactg tcttgctac ggccctttt ggtcaaact 
      ggcaagaca agtgcttcc gaaatcaaa gtcttcagt 
      cgggcctgc cataaccgg gctgatttt tgccacgca 
      tcccataca cacggaagc ggtggctgg ggttgtggg 
      ccgagctga tttggcagc atgcaattt ttatctatt 
      atcgttcct agcctacgt tacgcatac taagcatag 
      agattcgtt ttgttgtta tggtggtaa taaagaaga 
      caatatgaa gcagcttag aactttgaa gtaccagaa 
      gtttgttta tagtctgca aaaaaacgt tacgagttg 
      taattggcc aaagaggtt gggagtgag cgaggcttg 
      taataaaaa cgtcgtctt ccgaatgca tttgaagga 
      gaagaatga agcgtgttg tttgaaaag tagagtgag 
      gcaatggat gtacttggg tctgtgcat tctcctggc 
      tttggctgc gatgctgag caaggagac ggggatcac 
      agtgctggg gatgtgtat gatttacga cttcattta 
      ctgcccttc gttgaaaaa gagacaatg gcgcagttt 
      gctcgtcct gggagcagc ttccgttat ccttttagc 
      catccgctt gcaatcccg cgtgcccca gttctccac 
      cgctgacaa tccgacggc tcgcgagcc gaaactcct 
      cacccgctt gctcacctt catctacct tcccgtctt 
      caacttctg gctgctcct cgtccccaa caccctgag 
      ttttgactg gggcatgga cgccatccc ccgaatcac 
      cactctggg cgacagccg caacatcgt caccttctg 
      cttctactc gctgctctt cctcctggt gaaaaagag 
      cgtctcgtc gatgagatc gccgccgaa gacgtgccg 
      ctgctgcca cttggactt gtccgatgt acatacggt 
      taattgtcg gaacacgaa caataataa tacggcgca 
      ttcgacgtg cgtctgtct ttcgaaaag atcgacatc 
      gaattcctg ttgtgttat tttgttggc gctggcgtt 
      tctgactct tcctttttt gccggcgac caacttact 
      gttttatgt cgggtttgt ggtggccga gagggttct 
      ttatctacc cagtgcggg actgtgttt gatggtggg 
      actcggcgg ggctgcgct ttatcggaa gtatcgcac 
      gccgtttac tgtgggctt tattggagt tttgctaac 
      gttcagtgc taaaaccgt gctgagaaa caaagactg 
      gaccaacga ggaagcgct gtatcgggc cgccgtcaa 
      cgtcaaccc gcccaaagc gttcgggaa tttaggcag 
      cgttttgag cagtcaggg ccgaatcac ggaagccga 
      gtgggcatt ccgcaaggc gctacaatt ccgacccaa 
      catggccga tgtgcacta taatctggg cattctgct 
      ccaggcgag gcagcaatt gggcgaagc catcgagag 
      ttaccaacg agccattca ttttcgacc atcgctggc 
      actggccta tgtcaactt gggcgccgc gctgatatc 
      tgcaggcag gtgtcaaga agcagtgtc agttcttag 
      acaaggatc tcgtttaga cgggacggg tctgaggga 
      ccgacgcga gcacgaaac ggccagagt ttcggccct 
      cctgcagct gggagcttt gtattccga gcagggacg 
      actgcaaag ggctttagc ggcctacag agaagcggc 
      ctactcgct cccagagca ctatccacc acagagcgt 
      tttcaacgt gttgggaga gacactcgc ccgactcca 
      gcaggacga ggaagccga aaggtggta ccaagctgc 
      gctgaatgc acagcctga ccatgtgcc tgcccacat 
      aacttacgg caaactcct tgccaaaaa tgtgagccg 
      aacggcgga agccgaaca gtggtttcg caaagccca 
      aaggttggc cccccagga gcccagcgt ctaccacca 
      ttacggtga gttcctggc gtcgcggcg ccgctataa 
      agaagcgtc ggttatgta cgaaaaggc ggcagaatt 
      gcggccaca tgactacga gttggcggt ggcggcggc 
      gacggcgat gaggcaggc gggaaggca ccaggaggc 
      tgaacgctg gtacagaac ggcggtaga cctcaagcc 
      ttccgatgc tcgcagtca tacgaattt gggcgcgat 
      cctccacct gaacggcaa atatagcga agctgccag 
      cagctacaa ggaggcatt aaggcttca gccagacga 
      catcaccac gctgacgaa tctgcataa gctgcacag 
      cgtaatgac ctgacactg aaagcgttg aaaacgctc 
      cgggtttta ttatcaacg aagtaagga gaaattatc 
      tatgtgcta cgtgtgctt ctctacgcc gacgttttg 
      ggaccaccc cgtattgca acactcggc aaccacgag 
      actctgaca atatgaatg acgatcgat agtgatatt 
      tttgttaag aactgtatt ttttcagac gtcactttc 
      agcgtgata ttctgtaca taaggtacg ttgagtttc 
      ctcgatttt cattcggcg ttacgtttt tcttatgga 
      aaataaaat gtttacata tctttttat cagtgtaat 
      gtaattttt gaattggat aaaagcttc tcgactatt 
      attcccttg gaattgtaa gctttcaaa ataatatta 
      ttaccatat tgttggctt ttaatgggt cgtatattg 
      taattaaat ctgtattta attaaattg taaaataaa 
      gctttaact tattt
    • >TC032761 RB
      gcatcgaca taaaaccac gtgtaaaat ggcatcgaa 
      agaaaagta ctgctcaaa atccgaccg gggggcagt 
      aaaaatctc tgtaatcat tccccgggt ggttatgac 
      tcaagtaga gtgcaatag gagggctgt gagtggact 
      gcgtcgtaa aacaaaggc tccttctgc cggaggcat 
      gcgttcgca ctctacaca gttacggct cttcacatc 
      gtcaacacg gcaccgggt ttttgacgc ccgaagagg 
      ccactttga ccctgcgaa agcttcgtg tttgcgaga 
      taaatttca ctggggttc cgtcttgct cctccaggt 
      gaactccac aggtgacgc tctggaggc ggcacgcaa 
      tggacccga ctcgcttaa tgaactcgc tctcgtgaa 
      cggcgcccg cgaacgcac ccgaagatc ggagctttc 
      ctccggcaa aaagtcgcc cgaaccctt tttgccgcg 
      ctttgccga gattagcgc agctttgag ttgggcgca 
      acaagtcac ggtatcttc gcccatcga tgtgctgac 
      ccccagcat ggattatgt tagctctct ctgctgtct 
      tctcgcctt cgtcctcta ctacaacac gctggatgc 
      cggattcgt ttacgacga ccggcgggc gattctgag 
      caaccccga cctgctgcc gaagacgcc atgggcgcg 
      cctcttgga aaacgactt ctgggggac tcctctcag 
      cgacagcgg ctctcacgg ctcgtatcg tccgctgtg 
      cgttctaac cttccgcct caattacct tctgggagg 
      tttccaacc gtggggcta ccacctcgt caacgtgct 
      tttacactg tcttgctac ggccctttt ggtcaaact 
      ggcaagaca agtgcttcc gaaatcaaa gtcttcagt 
      cgggcctgc cataaccgg gctgatttt tgccacgca 
      tcccataca cacggaagc ggtggctgg ggttgtggg 
      ccgagctga tttggcagc atgcaattt ttatctatt 
      atcgttcct agcctacgt tacgcatac taagcatag 
      agattcgtt ttgttgtta tggtggtaa taaagaaga 
      caatatgaa gcagcttag aactttgaa gtaccagaa 
      gtttgttta tagtctgca aaaaaacgt tacgagttg 
      taattggcc aaagaggtt gggagtgag cgaggcttg 
      taataaaaa cgtcgtctt ccgaatgca tttgaagga 
      gaagaatga agcgtgttg tttgaaaag tagagtgag 
      gcaatggat gtacttggg tctgtgcat tctcctggc 
      tttggctgc gatgctgag caaggagac ggggatcac 
      agtgctggg gatgtgtat gatttacga cttcattta 
      ctgcccttc gttgaaaaa gagacaatg gcgcagttt 
      gctcgtcct gggagcagc ttccgttat ccttttagc 
      catccgctt gcaatcccg cgtgcccca gttctccac 
      cgctgacaa tccgacggc tcgcgagcc gaaactcct 
      cacccgctt gctcacctt catctacct tcccgtctt 
      caacttctg gctgctcct cgtccccaa caccctgag 
      ttttgactg gggcatgga cgccatccc ccgaatcac 
      cactctggg cgacagccg caacatcgt caccttctg 
      cttctactc gctgctctt cctcctggt gaaaaagag 
      cgtctcgtc gatgagatc gccgccgaa gacgtgccg 
      ctgctgcca cttggactt gtccgatgt acatacggt 
      taattgtcg gaacacgaa caataataa tacggcgca 
      ttcgacgtg cgtctgtct ttcgaaaag atcgacatc 
      gaattcctg ttgtgttat tttgttggc gctggcgtt 
      tctgactct tcctttttt gccggcgac caacttact 
      gttttatgt cgggtttgt ggtggccga gagggttct 
      ttatctacc cagtgcggg actgtgttt gatggtggg 
      actcggcgg ggctgcgct ttatcggaa gtatcgcac 
      gccgtttac tgtgggctt tattggagt tttgctaac 
      gttcagtgc taaaaccgt gctgagaaa caaagactg 
      gaccaacga ggaagcgct gtatcgggc cgccgtcaa 
      cgtcaaccc gcccaaagc gttcgggaa tttaggcag 
      cgttttgag cagtcaggg ccgaatcac ggaagccga 
      gtgggcatt ccgcaaggc gctacaatt ccgacccaa 
      catggccga tgtgcacta taatctggg cattctgct 
      ccaggcgag gcagcaatt gggcgaagc catcgagag 
      ttaccaacg agccattca ttttcgacc atcgctggc 
      actggccta tgtcaactt gggcgccgc gctgatatc 
      tgcaggcag gtgtcaaga agcagtgtc agttcttag 
      acaaggatc tcgtttaga cgggacggg tctgaggga 
      ccgacgcga gcacgaaac ggccagagt ttcggccct 
      cctgcagct gggagcttt gtattccga gcagggacg 
      actgcaaag ggctttagc ggcctacag agaagcggc 
      ctactcgct cccagagca ctatccacc acagagcgt 
      tttcaacgt gttgggaga gacactcgc ccgactcca 
      gcaggacga ggaagccga aaggtggta ccaagctgc 
      gctgaatgc acagcctga ccatgtgcc tgcccacat 
      aacttacgg caaactcct tgccaaaaa tgtgagccg 
      aacggcgga agccgaaca gtggtttcg caaagccca 
      aaggttggc cccccagga gcccagcgt ctaccacca 
      ttacggtga gttcctggc gtcgcggcg ccgctataa 
      agaagcgtc ggttatgta cgaaaaggc ggcagaatt 
      gcggccaca tgactacga gttggcggt ggcggcggc 
      gacggcgat gaggcaggc gggaaggca ccaggaggc 
      tgaacgctg gtacagaac ggcggtaga cctcaagcc 
      ttccgtgag tagattaaa caactttat tatagggaa 
      gctttcgat gcacctctg tgaactttt attcggatt 
      tgatagagc gcgccgatc gaagcggct tgactaaag 
      aaagaaatc tcttagaag gatatgata aaatggaaa 
      tgaagctct gcagtgatt ctcgtgact tagtcgtgg 
      cagcttgtt aagttggcg gaggatttc aacgcttcg 
      cctaaaaaa cgcgccttt ttctcctaa atcgcctta 
      attacgatt atgacattt ccagttggg gcaaaggaa 
      aattggagg caattttgc caaaagtct gttctgtta 
      attgtatat ttaatgagt tttcatctt catatcgtt 
      cagaattta attatacgc gaataaagg gttaagagg 
      ttcttgtaa ctgaaacgg cattaagac atttgccga 
      aaaaatcat ctcggcgta ttacgagag tactttgtc 
      gatattagc agcgtaaag tccttgtaa tcccggtag 
      aactttctc tttcaggat gctcgcagt catacgaat 
      ttgggcgcg atcctccac ctgaacggc aaatatagc 
      gaagctgcc agcagctac aaggaggca ttaaggctt 
      cagccagac gacatcacc acgctgacg aatctgcat 
      aagctgcac agcgtaatg acctgacac tgaaagcgt 
      tgaaaacgc tccgggttt tattatcaa cgaagtaag 
      gagaaatta tctatgtgc tacgtgtgc ttctctacg 
      ccgacgttt tgggaccac cccgtattg caacactcg 
      gcaaccacg agactctga caatatgaa tgacgatcg 
      atagtgata tttttgtta agaactgta ttttttcag 
      acgtcactt tcagcgtga tattctgta cataaggta 
      cgttgagtt tcctcgatt ttcattcgg cgttacgtt 
      tttcttatg gaaaataaa atgtttaca tatcttttt 
      atcagtgta atgtaattt ttgaattgg ataaaagct 
      tctcgacta ttattccct tggaattgt aagctttca 
      aaataatat tattaccat attgttggc ttttaatgg 
      gtcgtatat tgtaattaa atctgtatt taattaaat 
      tgtaaaata aagctttaa cttattt